m dtt Search Results


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  • 99
    Thermo Fisher m dtt
    Polysulfides inhibit PTEN by sulfane sulfur addition. (A) Recombinant PTEN-WT was <t>prereduced</t> with 20 m M <t>DTT</t> for 10 min, followed by buffer exchange into a nonreducing PTEN assay buffer and exposure to 100 μ M H 2 O 2 or NaHS
    M Dtt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore m dtt
    Effect of <t>TCA</t> on β-actin after <t>DTT</t> exposure. ELISA plates were coated with β-actin. Wells were exposed to either 5 m M DTT in PBSET or PBSET alone and thereafter to TCA or PBSET. Subsequently, the wells were exposed to biotin-maleimide,
    M Dtt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad m dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 340 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore m dtt dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dtt Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m dtt  (Roche)
    92
    Roche m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 2226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Boehringer Mannheim m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 628 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Research Products International m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Research Products International, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Carl Roth GmbH m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 92/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 2052 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    INDOFINE m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by INDOFINE, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Nacalai m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m dtt  (Abcam)
    97
    Abcam m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    BioVision m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by BioVision, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Trion Research m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Trion Research, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m dtt  (ATUM)
    90
    ATUM m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by ATUM, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 6 article reviews
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    92
    PerkinElmer m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 270 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m dtt  (Sanyo)
    92
    Sanyo m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Sanyo, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Duchefa m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Duchefa, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Gold Biotechnology Inc m dtt
    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m <t>Tricine-NaOH,</t> pH 7.5, 5 m m <t>DTT,</t> 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ
    M Dtt, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Polysulfides inhibit PTEN by sulfane sulfur addition. (A) Recombinant PTEN-WT was prereduced with 20 m M DTT for 10 min, followed by buffer exchange into a nonreducing PTEN assay buffer and exposure to 100 μ M H 2 O 2 or NaHS

    Journal: Antioxidants & Redox Signaling

    Article Title: Polysulfides Link H2S to Protein Thiol Oxidation

    doi: 10.1089/ars.2012.5041

    Figure Lengend Snippet: Polysulfides inhibit PTEN by sulfane sulfur addition. (A) Recombinant PTEN-WT was prereduced with 20 m M DTT for 10 min, followed by buffer exchange into a nonreducing PTEN assay buffer and exposure to 100 μ M H 2 O 2 or NaHS

    Article Snippet: Typically, roGFP2-His was prereduced with 20 m M DTT for 10 min before the experiment, and passed through gel filtration columns (Thermo Scientific) pre-equilibrated with the roGFP2 assay buffer to remove DTT immediately before the start of the assay.

    Techniques: Recombinant, Buffer Exchange

    Effect of TCA on β-actin after DTT exposure. ELISA plates were coated with β-actin. Wells were exposed to either 5 m M DTT in PBSET or PBSET alone and thereafter to TCA or PBSET. Subsequently, the wells were exposed to biotin-maleimide,

    Journal: Antioxidants & Redox Signaling

    Article Title: Is Trichloroacetic Acid an Insufficient Sample Quencher of Redox Reactions?

    doi: 10.1089/ars.2012.4949

    Figure Lengend Snippet: Effect of TCA on β-actin after DTT exposure. ELISA plates were coated with β-actin. Wells were exposed to either 5 m M DTT in PBSET or PBSET alone and thereafter to TCA or PBSET. Subsequently, the wells were exposed to biotin-maleimide,

    Article Snippet: One or 5 m M DTT (Sigma-Aldrich) diluted in PBST or 100% TCA, respectively, was added to the wells and incubated for 15 min at room temperature.

    Techniques: Enzyme-linked Immunosorbent Assay

    The activity of TCA as a quencher of β-actin oxidation by diamide. ELISA plates were coated with β-actin. The wells were then reduced by 5 m M DTT and exposed to 2 or 10 m M diamide diluted to in PBST or 100% TCA. Wells that

    Journal: Antioxidants & Redox Signaling

    Article Title: Is Trichloroacetic Acid an Insufficient Sample Quencher of Redox Reactions?

    doi: 10.1089/ars.2012.4949

    Figure Lengend Snippet: The activity of TCA as a quencher of β-actin oxidation by diamide. ELISA plates were coated with β-actin. The wells were then reduced by 5 m M DTT and exposed to 2 or 10 m M diamide diluted to in PBST or 100% TCA. Wells that

    Article Snippet: One or 5 m M DTT (Sigma-Aldrich) diluted in PBST or 100% TCA, respectively, was added to the wells and incubated for 15 min at room temperature.

    Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay

    The activity of TCA as quencher of β-actin reduction by DTT. ELISA plates were coated with β-actin. The wells were then reduced by 1 m M or 5 m M DTT in PBST or TCA, respectively. Subsequently, the wells were exposed to biotin-maleimide,

    Journal: Antioxidants & Redox Signaling

    Article Title: Is Trichloroacetic Acid an Insufficient Sample Quencher of Redox Reactions?

    doi: 10.1089/ars.2012.4949

    Figure Lengend Snippet: The activity of TCA as quencher of β-actin reduction by DTT. ELISA plates were coated with β-actin. The wells were then reduced by 1 m M or 5 m M DTT in PBST or TCA, respectively. Subsequently, the wells were exposed to biotin-maleimide,

    Article Snippet: One or 5 m M DTT (Sigma-Aldrich) diluted in PBST or 100% TCA, respectively, was added to the wells and incubated for 15 min at room temperature.

    Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay

    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m DTT with apo-AcnB (0.2

    Journal: The Journal of Biological Chemistry

    Article Title: Iron-Sulfur (Fe-S) Cluster Assembly

    doi: 10.1074/jbc.M110.127449

    Figure Lengend Snippet: Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m DTT with apo-AcnB (0.2

    Article Snippet: For the experiment in the absence of DTT during the FeS transfer, apo-aconitase B was first pretreated with 5 m m DTT for 30 min, before desalting the protein solution via a MicroBiospin column (Bio-Rad).

    Techniques: Incubation

    Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m Tricine-NaOH, pH 7.5, 5 m m DTT, 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ

    Journal:

    Article Title: Iron-Sulfur Cluster Biogenesis in Chloroplasts. Involvement of the Scaffold Protein CpIscA 1

    doi: 10.1104/pp.104.058602

    Figure Lengend Snippet: Purified CpIscA can acquire an Fe-S cluster. A, Gel filtration analysis. CpIscA (300 μ g) was incubated with CpNifS (75 μ g) in reconstitution buffer (50 m m Tricine-NaOH, pH 7.5, 5 m m DTT, 1 m m l -Cys, 1 m m ferrous ammonium sulfate, 20 μ

    Article Snippet: The role of IscA in Fe-S cluster assembly was assayed by incubating IscA (30 μ g) with NifS (7.5 μ g) for 30 min in reconstitution buffer containing 50 m m Tricine-NaOH, pH 7.5, 5 m m DTT (Roche Diagnostics, Palo Alto, CA), 1 m m l -Cys (Sigma, St. Louis), 1 m m ferrous ammonium sulfate, and 20 μ m PLP.

    Techniques: Purification, Filtration, Incubation