m dithiothreitol Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher m dithiothreitol
    Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) <t>dithiothreitol.</t> Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.
    M Dithiothreitol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 789 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Thermo Fisher
    Average 99 stars, based on 789 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Millipore m dl dithiothreitol
    Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) <t>dithiothreitol.</t> Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.
    M Dl Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dl dithiothreitol/product/Millipore
    Average 99 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    m dl dithiothreitol - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Bio-Rad
    Average 99 stars, based on 199 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    92
    Boehringer Mannheim m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Boehringer Mannheim
    Average 92 stars, based on 86 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    90
    Promega m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 434 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Promega
    Average 90 stars, based on 434 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    92
    Euromedex m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by Euromedex, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Euromedex
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Gold Biotechnology Inc m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Gold Biotechnology Inc
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Biosynth m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by Biosynth, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Biosynth
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    91
    Duchefa m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by Duchefa, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Duchefa
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    92
    Applichem m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by Applichem, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Applichem
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Fisher Scientific m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Fisher Scientific
    Average 92 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    TaKaRa m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/TaKaRa
    Average 92 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Roche m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 1402 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Roche
    Average 92 stars, based on 1402 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    90
    GERBU Biotechnik GmbH m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by GERBU Biotechnik GmbH, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/GERBU Biotechnik GmbH
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    92
    GE Healthcare m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 632 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/GE Healthcare
    Average 92 stars, based on 632 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Valiant m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Valiant
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    90
    Research Products International m dithiothreitol
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol, supplied by Research Products International, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Research Products International
    Average 90 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc m dithiothreitol dtt
    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m <t>DTT</t> with <t>apo-AcnB</t> (0.2
    M Dithiothreitol Dtt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol dtt/product/Cell Signaling Technology Inc
    Average 96 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol dtt - by Bioz Stars, 2020-05
    96/100 stars
      Buy from Supplier

    Image Search Results


    Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) dithiothreitol. Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Redox Regulation of Methionine Aminopeptidase 2 Activity *

    doi: 10.1074/jbc.M114.554253

    Figure Lengend Snippet: Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) dithiothreitol. Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.

    Article Snippet: Just before use, the active site disulfide bond of the oxidoreductase was reduced with 50 m m dithiothreitol and 50 m m tris-(2-carboxyethyl)phosphine (TCEP) for 1 h at 25 °C, and the reducing agents were removed by two passes through Zeba spin desalting columns equilibrated with phosphate-buffered saline (Thermo Scientific).

    Techniques: Labeling, SDS Page

    Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m DTT with apo-AcnB (0.2

    Journal: The Journal of Biological Chemistry

    Article Title: Iron-Sulfur (Fe-S) Cluster Assembly

    doi: 10.1074/jbc.M110.127449

    Figure Lengend Snippet: Iron-sulfur cluster transfer from SufBC 2 D to AcnB. Holo-SufBC 2 D complex (0.3 nmol), [Fe-S] ( gray bars ), or [Fe-S] + FADH 2 ( hatched bars ), was co-incubated in 10 μl of 50 m m Tris-HCl, pH 7.6, with ( a ) and without ( b ) 5 m m DTT with apo-AcnB (0.2

    Article Snippet: For the experiment in the absence of DTT during the FeS transfer, apo-aconitase B was first pretreated with 5 m m DTT for 30 min, before desalting the protein solution via a MicroBiospin column (Bio-Rad).

    Techniques: Incubation