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  • lys1  (ATCC)
    86
    ATCC lys1
    Lys1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Agilent technologies anti lys1
    Anti Lys1, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC matα lys1
    Strains used in this study
    Matα Lys1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore lys1 sf271 k lys1 femsyr 1421 26 11 01 body
    Strains used in this study
    Lys1 Sf271 K Lys1 Femsyr 1421 26 11 01 Body, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher s cerevisiae by4742 ∆ lys1
    ( a ) Confocal imaging of Lyp1-YPet and Lyp1(62–590)-YPet expressing cells and grown in the presence and absence of lysine. ( b ) Lysine transport rate of wild-type Lyp1 and Lyp1(62-590) expressing cells grown in the presence and absence of lysine. ( c,d ) Effect of FCCP (circles) and 100 mM lysine (500x fold excess of unlabeled solute, triangles) on the uptake of lysine into cells overexpressing Lyp1(62-590)-YPet; time-point of addition is indicated by arrows. ( c ) In S. cerevisiae <t>BY4742,</t> the initial rates of export expressed (as mM lysine*min −1 and SEM calculated) were 0.30 ± 0.02 and 0.28 ± 0.07 for FCCP treatment and lysine chase, respectively. ( d ) For S. cerevisiae BY4742 ∆vba1, ∆vba2, ∆Lys1 , the respective values were 0.25 ± 0.02 and 0.36 ± 0.04 mM lysine*min −1 . The rates of efflux and SEM are based on biological replicates (n = 3), and the observed differences are not significant.
    S Cerevisiae By4742 ∆ Lys1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Strains used in this study

    Journal: Yeast (Chichester, England)

    Article Title: Proteolytic processing of certain CaaX motifs can occur in the absence of the Rce1p and Ste24p CaaX proteases

    doi: 10.1002/yea.1678

    Figure Lengend Snippet: Strains used in this study

    Article Snippet: IH1793 , MATα lys1 , ATCC 204279.

    Techniques:

    ( a ) Confocal imaging of Lyp1-YPet and Lyp1(62–590)-YPet expressing cells and grown in the presence and absence of lysine. ( b ) Lysine transport rate of wild-type Lyp1 and Lyp1(62-590) expressing cells grown in the presence and absence of lysine. ( c,d ) Effect of FCCP (circles) and 100 mM lysine (500x fold excess of unlabeled solute, triangles) on the uptake of lysine into cells overexpressing Lyp1(62-590)-YPet; time-point of addition is indicated by arrows. ( c ) In S. cerevisiae BY4742, the initial rates of export expressed (as mM lysine*min −1 and SEM calculated) were 0.30 ± 0.02 and 0.28 ± 0.07 for FCCP treatment and lysine chase, respectively. ( d ) For S. cerevisiae BY4742 ∆vba1, ∆vba2, ∆Lys1 , the respective values were 0.25 ± 0.02 and 0.36 ± 0.04 mM lysine*min −1 . The rates of efflux and SEM are based on biological replicates (n = 3), and the observed differences are not significant.

    Journal: Scientific Reports

    Article Title: Asymmetry in inward- and outward-affinity constant of transport explain unidirectional lysine flux in Saccharomyces cerevisiae

    doi: 10.1038/srep31443

    Figure Lengend Snippet: ( a ) Confocal imaging of Lyp1-YPet and Lyp1(62–590)-YPet expressing cells and grown in the presence and absence of lysine. ( b ) Lysine transport rate of wild-type Lyp1 and Lyp1(62-590) expressing cells grown in the presence and absence of lysine. ( c,d ) Effect of FCCP (circles) and 100 mM lysine (500x fold excess of unlabeled solute, triangles) on the uptake of lysine into cells overexpressing Lyp1(62-590)-YPet; time-point of addition is indicated by arrows. ( c ) In S. cerevisiae BY4742, the initial rates of export expressed (as mM lysine*min −1 and SEM calculated) were 0.30 ± 0.02 and 0.28 ± 0.07 for FCCP treatment and lysine chase, respectively. ( d ) For S. cerevisiae BY4742 ∆vba1, ∆vba2, ∆Lys1 , the respective values were 0.25 ± 0.02 and 0.36 ± 0.04 mM lysine*min −1 . The rates of efflux and SEM are based on biological replicates (n = 3), and the observed differences are not significant.

    Article Snippet: For the construction of S. cerevisiae BY4742 ∆ lys1, ∆vba1, ∆vba2 we made use of the ura3 selection marker and the ability for its counter selection on 5 fluoro-orotic acid (5FOA, Thermo Fisher Scientific, Waltham, MA, USA) as described by .

    Techniques: Imaging, Expressing