Journal: Yeast (Chichester, England)

Article Title: Proteolytic processing of certain CaaX motifs can occur in the absence of the Rce1p and Ste24p CaaX proteases

doi: 10.1002/yea.1678

Figure Lengend Snippet: Strains used in this study

Article Snippet: IH1793 , MATα lys1 , ATCC 204279.

Techniques:

Journal: Scientific Reports

Article Title: Asymmetry in inward- and outward-affinity constant of transport explain unidirectional lysine flux in Saccharomyces cerevisiae

doi: 10.1038/srep31443

Figure Lengend Snippet: ( a ) Confocal imaging of Lyp1-YPet and Lyp1(62–590)-YPet expressing cells and grown in the presence and absence of lysine. ( b ) Lysine transport rate of wild-type Lyp1 and Lyp1(62-590) expressing cells grown in the presence and absence of lysine. ( c,d ) Effect of FCCP (circles) and 100 mM lysine (500x fold excess of unlabeled solute, triangles) on the uptake of lysine into cells overexpressing Lyp1(62-590)-YPet; time-point of addition is indicated by arrows. ( c ) In S. cerevisiae BY4742, the initial rates of export expressed (as mM lysine*min −1 and SEM calculated) were 0.30 ± 0.02 and 0.28 ± 0.07 for FCCP treatment and lysine chase, respectively. ( d ) For S. cerevisiae BY4742 ∆vba1, ∆vba2, ∆Lys1 , the respective values were 0.25 ± 0.02 and 0.36 ± 0.04 mM lysine*min −1 . The rates of efflux and SEM are based on biological replicates (n = 3), and the observed differences are not significant.

Article Snippet: For the construction of S. cerevisiae BY4742 ∆ lys1, ∆vba1, ∆vba2 we made use of the ura3 selection marker and the ability for its counter selection on 5 fluoro-orotic acid (5FOA, Thermo Fisher Scientific, Waltham, MA, USA) as described by .

Techniques: Imaging, Expressing