luria bertani broth Search Results


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  • 95
    Millipore luria bertani lb medium
    Luria Bertani Lb Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 725 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco luria bertani broth
    Biofilm formation by A . pleuropneumoniae serotype 1 reference strain S4074 grown in different culture media using the crystal violet staining protocol described in Materials and methods. LB: <t>Luria-Bertani;</t> TSB: tryptic soy broth; M-H: Mueller Hinton; BHI: brain heart infusion.
    Luria Bertani Broth, supplied by Difco, used in various techniques. Bioz Stars score: 97/100, based on 849 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific luria bertani broth
    Biofilm formation by A . pleuropneumoniae serotype 1 reference strain S4074 grown in different culture media using the crystal violet staining protocol described in Materials and methods. LB: <t>Luria-Bertani;</t> TSB: tryptic soy broth; M-H: Mueller Hinton; BHI: brain heart infusion.
    Luria Bertani Broth, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson luria bertani broth
    Schematic for capturing scatter pattern of colony with optical sensor and selection of optimal media. (A) Schematics of BARDOT to acquire scatter patterns of colonies. (B) Evaluation of different non-selective agar media to capture scatter pattern of Salmonella enterica serovar Typhimurium 13ENT0899 colonies. Brain–heart infusion agar (BHIA); <t>Luria–Bertani</t> agar (LBA); Nutrient agar (NA); Tryptic soy agar (TSA). Scatter patterns were captured when the colony diameter reached to 1.2 ± 0.1 mm after 10–12 h of incubation.
    Luria Bertani Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 96/100, based on 494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellgro luria bertani broth
    Schematic for capturing scatter pattern of colony with optical sensor and selection of optimal media. (A) Schematics of BARDOT to acquire scatter patterns of colonies. (B) Evaluation of different non-selective agar media to capture scatter pattern of Salmonella enterica serovar Typhimurium 13ENT0899 colonies. Brain–heart infusion agar (BHIA); <t>Luria–Bertani</t> agar (LBA); Nutrient agar (NA); Tryptic soy agar (TSA). Scatter patterns were captured when the colony diameter reached to 1.2 ± 0.1 mm after 10–12 h of incubation.
    Luria Bertani Broth, supplied by Cellgro, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher luria bertani broth
    Bacterial growth curve of ( A ) Escherichia coli and ( B ) Staphylococcus aureus in LB liquid medium in the presence of Ag-MSNs with different concentrations. These data represent three separate experiments and are presented as mean values±SD. Abbreviations: Ag-MSNs, nanosilver-decorated mesoporous silica nanoparticles; LB, <t>Luria–Bertani.</t>
    Luria Bertani Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 582 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co luria bertani broth
    Bacterial growth curve of ( A ) Escherichia coli and ( B ) Staphylococcus aureus in LB liquid medium in the presence of Ag-MSNs with different concentrations. These data represent three separate experiments and are presented as mean values±SD. Abbreviations: Ag-MSNs, nanosilver-decorated mesoporous silica nanoparticles; LB, <t>Luria–Bertani.</t>
    Luria Bertani Broth, supplied by Merck & Co, used in various techniques. Bioz Stars score: 94/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore luria bertani broth
    Growth of DH5α cells containing pET28a, pGreenII, and pMET1-03. (A) Cell morphology. Aliquots of culture were viewed using a light microscope under phase contrast at 100 × magnification with an oil immersion objective. A graticule was used to provide a measuring scale. The length of the sides of the visible squares was 50 µm. The length of a typical E. coli cell is 2 µm. (B) The growth of E. coli using overnight cultures as an inoculum. 50 ml of LB media (containing 50 µg/ml kanamycin) within a 250 ml Erlenmeyer flask was inoculated with 0.2 OD 600 units of cells from a 5 ml overnight culture (see Materials and Methods ). Growth was monitored by measuring OD 600 values. The data-points for pET28a and pGreenII are represented by diamonds and squares, respectively. (C) Colony morphology. The pictures are of primary transformants following overnight incubation. The graduations at the bottom of each image in this panel correspond to 1 mm. (D) Restriction enzyme analysis of a selection of plasmids isolated from mutants that produce large colonies. The lane labeled M contains the 1 kb Plus DNA Ladder (Life Technologies). The mutants are in lanes 1–16. Numbering on the left of the panel indicates the expected sizes of the two fragments produced by Eco RI digestion of pMET1-03. The smaller of the two fragments corresponds to the MET1 cassette destined for plants. Labels that are outlined indicate plasmids with obvious rearrangements. The gel used for electrophoresis was composed of 0.8% [w/v] agarose and stained with ethidium bromide. LB, <t>Luria</t> <t>Bertani;</t> OD, optical density.
    Luria Bertani Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 605 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai luria bertani broth
    Growth of DH5α cells containing pET28a, pGreenII, and pMET1-03. (A) Cell morphology. Aliquots of culture were viewed using a light microscope under phase contrast at 100 × magnification with an oil immersion objective. A graticule was used to provide a measuring scale. The length of the sides of the visible squares was 50 µm. The length of a typical E. coli cell is 2 µm. (B) The growth of E. coli using overnight cultures as an inoculum. 50 ml of LB media (containing 50 µg/ml kanamycin) within a 250 ml Erlenmeyer flask was inoculated with 0.2 OD 600 units of cells from a 5 ml overnight culture (see Materials and Methods ). Growth was monitored by measuring OD 600 values. The data-points for pET28a and pGreenII are represented by diamonds and squares, respectively. (C) Colony morphology. The pictures are of primary transformants following overnight incubation. The graduations at the bottom of each image in this panel correspond to 1 mm. (D) Restriction enzyme analysis of a selection of plasmids isolated from mutants that produce large colonies. The lane labeled M contains the 1 kb Plus DNA Ladder (Life Technologies). The mutants are in lanes 1–16. Numbering on the left of the panel indicates the expected sizes of the two fragments produced by Eco RI digestion of pMET1-03. The smaller of the two fragments corresponds to the MET1 cassette destined for plants. Labels that are outlined indicate plasmids with obvious rearrangements. The gel used for electrophoresis was composed of 0.8% [w/v] agarose and stained with ethidium bromide. LB, <t>Luria</t> <t>Bertani;</t> OD, optical density.
    Luria Bertani Broth, supplied by Nacalai, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories luria bertani broth
    Growth of DH5α cells containing pET28a, pGreenII, and pMET1-03. (A) Cell morphology. Aliquots of culture were viewed using a light microscope under phase contrast at 100 × magnification with an oil immersion objective. A graticule was used to provide a measuring scale. The length of the sides of the visible squares was 50 µm. The length of a typical E. coli cell is 2 µm. (B) The growth of E. coli using overnight cultures as an inoculum. 50 ml of LB media (containing 50 µg/ml kanamycin) within a 250 ml Erlenmeyer flask was inoculated with 0.2 OD 600 units of cells from a 5 ml overnight culture (see Materials and Methods ). Growth was monitored by measuring OD 600 values. The data-points for pET28a and pGreenII are represented by diamonds and squares, respectively. (C) Colony morphology. The pictures are of primary transformants following overnight incubation. The graduations at the bottom of each image in this panel correspond to 1 mm. (D) Restriction enzyme analysis of a selection of plasmids isolated from mutants that produce large colonies. The lane labeled M contains the 1 kb Plus DNA Ladder (Life Technologies). The mutants are in lanes 1–16. Numbering on the left of the panel indicates the expected sizes of the two fragments produced by Eco RI digestion of pMET1-03. The smaller of the two fragments corresponds to the MET1 cassette destined for plants. Labels that are outlined indicate plasmids with obvious rearrangements. The gel used for electrophoresis was composed of 0.8% [w/v] agarose and stained with ethidium bromide. LB, <t>Luria</t> <t>Bertani;</t> OD, optical density.
    Luria Bertani Broth, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA luria bertani broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Luria Bertani Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 94/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carl Roth GmbH luria bertani broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Luria Bertani Broth, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Teknova luria bertani broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Luria Bertani Broth, supplied by Teknova, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad luria bertani broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Luria Bertani Broth, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Amresco luria bertani broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Luria Bertani Broth, supplied by Amresco, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Formedium luria bertani broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
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    Interchim luria bertani broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
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    Bioworld Antibodies luria bertani broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Luria Bertani Broth, supplied by Bioworld Antibodies, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher luria bertoni broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
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    Alpha Biosciences luria bertania broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Luria Bertania Broth, supplied by Alpha Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco luria bertoni broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
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    Becton Dickinson difco luria bertani broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Difco Luria Bertani Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 78/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Lonza luria bertani lb broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
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    Applichem luria bertani lb broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
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    Becton Dickinson liquid luria bertani broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
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    Melford Laboratories luria bertani lb broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
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    BioShop luria bertani lb broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
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    Valiant luria bertani lb broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Luria Bertani Lb Broth, supplied by Valiant, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EM Science Inc luria bertani lb broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Luria Bertani Lb Broth, supplied by EM Science Inc, used in various techniques. Bioz Stars score: 90/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore luria bertani lennox broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
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    Alpha Biosciences luria bertani lb broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Luria Bertani Lb Broth, supplied by Alpha Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    luria bertani lb broth - by Bioz Stars, 2020-02
    90/100 stars
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    Fisher Bioreagents luria bertani lb broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Luria Bertani Lb Broth, supplied by Fisher Bioreagents, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 19 article reviews
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    luria bertani lb broth - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

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    Biofilm formation by A . pleuropneumoniae serotype 1 reference strain S4074 grown in different culture media using the crystal violet staining protocol described in Materials and methods. LB: Luria-Bertani; TSB: tryptic soy broth; M-H: Mueller Hinton; BHI: brain heart infusion.

    Journal: Veterinary Research

    Article Title: Effects of growth conditions on biofilm formation by Actinobacillus pleuropneumoniae

    doi: 10.1051/vetres/2009051

    Figure Lengend Snippet: Biofilm formation by A . pleuropneumoniae serotype 1 reference strain S4074 grown in different culture media using the crystal violet staining protocol described in Materials and methods. LB: Luria-Bertani; TSB: tryptic soy broth; M-H: Mueller Hinton; BHI: brain heart infusion.

    Article Snippet: A colony was transferred into 5 mL of Luria-Bertani broth (LB; Difco), tryptic soy broth (TSB; Difco), Mueller Hinton broth (MH; Difco) or BHI (BHI-A; Difco or BHI-B; Oxoid Ltd, Basingstoke, Hampshire, UK) with 5 μg/mL NAD and incubated at 37 °C overnight with agitation.

    Techniques: Staining

    Heat map of microarray analysis and the corresponding values of fold change for the 70 significantly upregulated or downregulated genes of in vitro M cells and Caco-2 cells after infection with wild-type (WT) S . Typhimurium and Δ speG or remaining uninfected for 1 h. We found 70 genes of in vitro M cells and Caco-2 cells that were significantly upregulated ( > 2-fold change, red bricks) after treatment with WT S . Typhimurium SL1344, the speG -deleted mutant (Δ speG ), and only Luria-Bertani broth (LB). These 70 genes were classified into 8 groups according to their relative or potential functions. Red bricks represent higher gene expression levels compared with average levels (black bricks).

    Journal: PLoS ONE

    Article Title: Impacts of Salmonella enterica Serovar Typhimurium and Its speG Gene on the Transcriptomes of In Vitro M Cells and Caco-2 Cells

    doi: 10.1371/journal.pone.0153444

    Figure Lengend Snippet: Heat map of microarray analysis and the corresponding values of fold change for the 70 significantly upregulated or downregulated genes of in vitro M cells and Caco-2 cells after infection with wild-type (WT) S . Typhimurium and Δ speG or remaining uninfected for 1 h. We found 70 genes of in vitro M cells and Caco-2 cells that were significantly upregulated ( > 2-fold change, red bricks) after treatment with WT S . Typhimurium SL1344, the speG -deleted mutant (Δ speG ), and only Luria-Bertani broth (LB). These 70 genes were classified into 8 groups according to their relative or potential functions. Red bricks represent higher gene expression levels compared with average levels (black bricks).

    Article Snippet: For cell infections, the single colonies of each strains were aerobically grown in 3 mL of Luria–Bertani (LB) broth (Difco) at 37°C for 18 h to reach a bacterial density of 1 × 109 CFU/mL as overnight cultures.

    Techniques: Microarray, In Vitro, Infection, Mutagenesis, Expressing

    Schematic for capturing scatter pattern of colony with optical sensor and selection of optimal media. (A) Schematics of BARDOT to acquire scatter patterns of colonies. (B) Evaluation of different non-selective agar media to capture scatter pattern of Salmonella enterica serovar Typhimurium 13ENT0899 colonies. Brain–heart infusion agar (BHIA); Luria–Bertani agar (LBA); Nutrient agar (NA); Tryptic soy agar (TSA). Scatter patterns were captured when the colony diameter reached to 1.2 ± 0.1 mm after 10–12 h of incubation.

    Journal: PLoS ONE

    Article Title: Streptomycin Induced Stress Response in Salmonella enterica Serovar Typhimurium Shows Distinct Colony Scatter Signature

    doi: 10.1371/journal.pone.0135035

    Figure Lengend Snippet: Schematic for capturing scatter pattern of colony with optical sensor and selection of optimal media. (A) Schematics of BARDOT to acquire scatter patterns of colonies. (B) Evaluation of different non-selective agar media to capture scatter pattern of Salmonella enterica serovar Typhimurium 13ENT0899 colonies. Brain–heart infusion agar (BHIA); Luria–Bertani agar (LBA); Nutrient agar (NA); Tryptic soy agar (TSA). Scatter patterns were captured when the colony diameter reached to 1.2 ± 0.1 mm after 10–12 h of incubation.

    Article Snippet: The dehydrated BHI was purchased from Acumedia (Neogen, Lansing, MI, USA); and nutrient broth (NB), Luria-Bertani (LB) broth, and tryptic soy broth (TSB) were purchased from Becton Dickinson (Sparks, MD, USA).

    Techniques: Selection, Incubation

    Bacterial growth curve of ( A ) Escherichia coli and ( B ) Staphylococcus aureus in LB liquid medium in the presence of Ag-MSNs with different concentrations. These data represent three separate experiments and are presented as mean values±SD. Abbreviations: Ag-MSNs, nanosilver-decorated mesoporous silica nanoparticles; LB, Luria–Bertani.

    Journal: International Journal of Nanomedicine

    Article Title: Antibacterial and biodegradable tissue nano-adhesives for rapid wound closure

    doi: 10.2147/IJN.S177109

    Figure Lengend Snippet: Bacterial growth curve of ( A ) Escherichia coli and ( B ) Staphylococcus aureus in LB liquid medium in the presence of Ag-MSNs with different concentrations. These data represent three separate experiments and are presented as mean values±SD. Abbreviations: Ag-MSNs, nanosilver-decorated mesoporous silica nanoparticles; LB, Luria–Bertani.

    Article Snippet: Luria–Bertani (LB) broth was purchased from Thermo Fisher Scientific.

    Techniques:

    Growth of DH5α cells containing pET28a, pGreenII, and pMET1-03. (A) Cell morphology. Aliquots of culture were viewed using a light microscope under phase contrast at 100 × magnification with an oil immersion objective. A graticule was used to provide a measuring scale. The length of the sides of the visible squares was 50 µm. The length of a typical E. coli cell is 2 µm. (B) The growth of E. coli using overnight cultures as an inoculum. 50 ml of LB media (containing 50 µg/ml kanamycin) within a 250 ml Erlenmeyer flask was inoculated with 0.2 OD 600 units of cells from a 5 ml overnight culture (see Materials and Methods ). Growth was monitored by measuring OD 600 values. The data-points for pET28a and pGreenII are represented by diamonds and squares, respectively. (C) Colony morphology. The pictures are of primary transformants following overnight incubation. The graduations at the bottom of each image in this panel correspond to 1 mm. (D) Restriction enzyme analysis of a selection of plasmids isolated from mutants that produce large colonies. The lane labeled M contains the 1 kb Plus DNA Ladder (Life Technologies). The mutants are in lanes 1–16. Numbering on the left of the panel indicates the expected sizes of the two fragments produced by Eco RI digestion of pMET1-03. The smaller of the two fragments corresponds to the MET1 cassette destined for plants. Labels that are outlined indicate plasmids with obvious rearrangements. The gel used for electrophoresis was composed of 0.8% [w/v] agarose and stained with ethidium bromide. LB, Luria Bertani; OD, optical density.

    Journal: G3: Genes|Genomes|Genetics

    Article Title: An Improved Binary Vector and Escherichia coli Strain for Agrobacterium tumefaciens-Mediated Plant Transformation

    doi: 10.1534/g3.116.029405

    Figure Lengend Snippet: Growth of DH5α cells containing pET28a, pGreenII, and pMET1-03. (A) Cell morphology. Aliquots of culture were viewed using a light microscope under phase contrast at 100 × magnification with an oil immersion objective. A graticule was used to provide a measuring scale. The length of the sides of the visible squares was 50 µm. The length of a typical E. coli cell is 2 µm. (B) The growth of E. coli using overnight cultures as an inoculum. 50 ml of LB media (containing 50 µg/ml kanamycin) within a 250 ml Erlenmeyer flask was inoculated with 0.2 OD 600 units of cells from a 5 ml overnight culture (see Materials and Methods ). Growth was monitored by measuring OD 600 values. The data-points for pET28a and pGreenII are represented by diamonds and squares, respectively. (C) Colony morphology. The pictures are of primary transformants following overnight incubation. The graduations at the bottom of each image in this panel correspond to 1 mm. (D) Restriction enzyme analysis of a selection of plasmids isolated from mutants that produce large colonies. The lane labeled M contains the 1 kb Plus DNA Ladder (Life Technologies). The mutants are in lanes 1–16. Numbering on the left of the panel indicates the expected sizes of the two fragments produced by Eco RI digestion of pMET1-03. The smaller of the two fragments corresponds to the MET1 cassette destined for plants. Labels that are outlined indicate plasmids with obvious rearrangements. The gel used for electrophoresis was composed of 0.8% [w/v] agarose and stained with ethidium bromide. LB, Luria Bertani; OD, optical density.

    Article Snippet: 0179) as described were grown in Luria Bertani broth (Sigma) with kanamycin selection (50 µg/ml) and shaking (200 rpm) at 37°.

    Techniques: Light Microscopy, Incubation, Selection, Isolation, Labeling, Produced, Electrophoresis, Staining

    SDS-PAGE gels stained with Coomassie Blue for recombinant Rv1733c expressed in E. coli BL21 (DE3) in different media, IPTG concentrations and time points. A: 2x Yeast extract and Tryptone broth was used as culture medium; B: Terrific Broth medium; C: Super Broth medium; D: Luria-Bertani broth with 0.5% NaCl. lane 1: time 0 (non-induced), lane 2: IPTG = 0.1 mM Time = 7 h, lane 3: IPTG = 0.1 mM Time = 8 h, lane 4: IPTG = 0.1 mM Time = 9 h, lane 5: IPTG = 0.1 mM Time = 10 h, lane 6: IPTG = 0.2 mM Time = 7 h, lane 7: IPTG = 0.2 mM Time = 8 h, lane 8: IPTG = 0.2 mM Time = 9 h, lane 9: IPTG = 0.2 mM Time = 10 h, lane 10: full-range Rainbow molecular weight marker, lane 11: IPTG = 0.3 mM Time = 7 h, lane 12: IPTG = 0.3 mM Time = 8 h, lane 13: IPTG = 0.3 mM Time = 9 h, lane 14: IPTG = 0.3 mM Time = 10 h, lane 15: IPTG = 0.4 mM Time = 7 h, lane 16: IPTG = 0.4 mM Time = 8 h, lane 17: IPTG = 0.4 mM Time = 9 h, lane 18: IPTG = 0.4 mM Time = 10 h. The protein band is indicated with arrow.

    Journal: Iranian Journal of Microbiology

    Article Title: Cloning, optimization of induction conditions and purification of Mycobacterium tuberculosis Rv1733c protein expressed in Escherichia coli

    doi:

    Figure Lengend Snippet: SDS-PAGE gels stained with Coomassie Blue for recombinant Rv1733c expressed in E. coli BL21 (DE3) in different media, IPTG concentrations and time points. A: 2x Yeast extract and Tryptone broth was used as culture medium; B: Terrific Broth medium; C: Super Broth medium; D: Luria-Bertani broth with 0.5% NaCl. lane 1: time 0 (non-induced), lane 2: IPTG = 0.1 mM Time = 7 h, lane 3: IPTG = 0.1 mM Time = 8 h, lane 4: IPTG = 0.1 mM Time = 9 h, lane 5: IPTG = 0.1 mM Time = 10 h, lane 6: IPTG = 0.2 mM Time = 7 h, lane 7: IPTG = 0.2 mM Time = 8 h, lane 8: IPTG = 0.2 mM Time = 9 h, lane 9: IPTG = 0.2 mM Time = 10 h, lane 10: full-range Rainbow molecular weight marker, lane 11: IPTG = 0.3 mM Time = 7 h, lane 12: IPTG = 0.3 mM Time = 8 h, lane 13: IPTG = 0.3 mM Time = 9 h, lane 14: IPTG = 0.3 mM Time = 10 h, lane 15: IPTG = 0.4 mM Time = 7 h, lane 16: IPTG = 0.4 mM Time = 8 h, lane 17: IPTG = 0.4 mM Time = 9 h, lane 18: IPTG = 0.4 mM Time = 10 h. The protein band is indicated with arrow.

    Article Snippet: A single colony of the recombinant E. coli BL21 (DE3) was inoculated into 5 ml Luria-Bertani (LB) broth (Sigma-Aldrich, MO, USA) supplemented with 100 μg/ml ampicillin (Sigma-Aldrich) and grown overnight.

    Techniques: SDS Page, Staining, Recombinant, Molecular Weight, Marker

    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, Luria-Bertani; BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.

    Journal: PLoS ONE

    Article Title: A novel bacteriocin from Enterococcus faecalis 478 exhibits a potent activity against vancomycin-resistant enterococci

    doi: 10.1371/journal.pone.0186415

    Figure Lengend Snippet: Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, Luria-Bertani; BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.

    Article Snippet: The membranes were then cut and immersed in Luria-Bertani broth (LB) (Merck, KGaA, Darmstadt, Germany) supplemented with 10% fetal bovine serum and incubated with shaking at 37°C for 4 hours (200 rpm, Innova 4300 incubator shaker, New Brunswick Scientific, Edison, NJ).

    Techniques: Incubation