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    The SRE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the serum response element SRE
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    Thermo Fisher pmir report luciferase vector
    Pmir Report Luciferase Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmir report luciferase vector/product/Thermo Fisher
    Average 99 stars, based on 3112 article reviews
    Price from $9.99 to $1999.99
    pmir report luciferase vector - by Bioz Stars, 2021-01
    99/100 stars
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    Promega luciferase activity
    RhoA and acto-myosin regulate the activity of hSREBP1 and dSREBP. a Screening of low density lipoprotein receptor promoter-luciferase activity in MDA-MB-231 cells transfected with constructs expressing firefly (LDLR-Luc) and <t>renilla</t> (Rluc) luciferase and either control siRNA (siCTL) or siRNAs targeting genes encoding geranylgeranyl pyrophosphate transferase I (GGTase1) protein substrates. b Western blot analysis of MDA-MB-231 and Mahlavu cells 48 h after transfection with siCTL or RhoA targeting siRNAs (siR#1 and siR#2). Hsp90 was used as loading control. mSREBP indicates mature protein. c Western blot analyses of immunoprecipitated (IP) RhoA in MCF-10A cells treated DMSO (as control), 1 μM cerivastatin (STAT), 1 μM cerivastatin and 20 μM GGPP (STAT+GGPP), 5 μM GGTI-298 or 5 μM FTI-277. IgGs were used as IP control. d LDLR-Luc assay in MCF-10A cells 12 h after transfection with pcDNA3-GFP control plasmid, pcDNA3-GFP-RhoA G14V construct with a 6 h DMSO treatment, pcDNA3-GFP-RhoA G14V construct with a 6 h Latrunculin A (G14V + Lat.A) treatment, or pcDNA3-GFP-RhoA T19N construct. e LDLR-Luc assay in MCF-10A cells treated with either DMSO as control or C3 for 24 h. f LDLR-Luc assay in MCF-10A cells treated with either DMSO as control, Y-27632, or Blebbistatin (Blebbist.) for 24 h. g BODIPY 493/503 staining of lipid droplets (in red) in Mahlavu cells treated with either DMSO, C3 or Y-27632 for 24 h. Scale bar, 15 μm. h , i BODIPY 493/503 staining of lipid droplets (in red) and immunofluorescence analysis of dSREBP (in green) and phosphorylated myosin light chain (pMLC2, in magenta) in Drosophila larval fat body from h flies expressing either Luciferase or dRhoA RNAi , or dRhoA RNAi and treated with fatostatin (FTS) and i wild-type flies treated with either DMSO or Y-27632. Scale bar, 20 μm. Graphs bars in ( c – f ) represent mean ± s.d. of n = 3 biological replicates. Values in ( d – f ) are expressed as Relative Luminometer Units (RLU). Nuclei in ( h – i ) were stained with HOECHST (in blue). Blots and images are representative of n = 3 biological replicates. P value: * P
    Luciferase Activity, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 50121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase activity/product/Promega
    Average 99 stars, based on 50121 article reviews
    Price from $9.99 to $1999.99
    luciferase activity - by Bioz Stars, 2021-01
    99/100 stars
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    Promega dual luciferase reporter assay system
    RhoA and acto-myosin regulate the activity of hSREBP1 and dSREBP. a Screening of low density lipoprotein receptor promoter-luciferase activity in MDA-MB-231 cells transfected with constructs expressing firefly (LDLR-Luc) and <t>renilla</t> (Rluc) luciferase and either control siRNA (siCTL) or siRNAs targeting genes encoding geranylgeranyl pyrophosphate transferase I (GGTase1) protein substrates. b Western blot analysis of MDA-MB-231 and Mahlavu cells 48 h after transfection with siCTL or RhoA targeting siRNAs (siR#1 and siR#2). Hsp90 was used as loading control. mSREBP indicates mature protein. c Western blot analyses of immunoprecipitated (IP) RhoA in MCF-10A cells treated DMSO (as control), 1 μM cerivastatin (STAT), 1 μM cerivastatin and 20 μM GGPP (STAT+GGPP), 5 μM GGTI-298 or 5 μM FTI-277. IgGs were used as IP control. d LDLR-Luc assay in MCF-10A cells 12 h after transfection with pcDNA3-GFP control plasmid, pcDNA3-GFP-RhoA G14V construct with a 6 h DMSO treatment, pcDNA3-GFP-RhoA G14V construct with a 6 h Latrunculin A (G14V + Lat.A) treatment, or pcDNA3-GFP-RhoA T19N construct. e LDLR-Luc assay in MCF-10A cells treated with either DMSO as control or C3 for 24 h. f LDLR-Luc assay in MCF-10A cells treated with either DMSO as control, Y-27632, or Blebbistatin (Blebbist.) for 24 h. g BODIPY 493/503 staining of lipid droplets (in red) in Mahlavu cells treated with either DMSO, C3 or Y-27632 for 24 h. Scale bar, 15 μm. h , i BODIPY 493/503 staining of lipid droplets (in red) and immunofluorescence analysis of dSREBP (in green) and phosphorylated myosin light chain (pMLC2, in magenta) in Drosophila larval fat body from h flies expressing either Luciferase or dRhoA RNAi , or dRhoA RNAi and treated with fatostatin (FTS) and i wild-type flies treated with either DMSO or Y-27632. Scale bar, 20 μm. Graphs bars in ( c – f ) represent mean ± s.d. of n = 3 biological replicates. Values in ( d – f ) are expressed as Relative Luminometer Units (RLU). Nuclei in ( h – i ) were stained with HOECHST (in blue). Blots and images are representative of n = 3 biological replicates. P value: * P
    Dual Luciferase Reporter Assay System, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 146512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual luciferase reporter assay system/product/Promega
    Average 99 stars, based on 146512 article reviews
    Price from $9.99 to $1999.99
    dual luciferase reporter assay system - by Bioz Stars, 2021-01
    99/100 stars
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    Promega luciferase reporter vector
    RhoA and acto-myosin regulate the activity of hSREBP1 and dSREBP. a Screening of low density lipoprotein receptor promoter-luciferase activity in MDA-MB-231 cells transfected with constructs expressing firefly (LDLR-Luc) and <t>renilla</t> (Rluc) luciferase and either control siRNA (siCTL) or siRNAs targeting genes encoding geranylgeranyl pyrophosphate transferase I (GGTase1) protein substrates. b Western blot analysis of MDA-MB-231 and Mahlavu cells 48 h after transfection with siCTL or RhoA targeting siRNAs (siR#1 and siR#2). Hsp90 was used as loading control. mSREBP indicates mature protein. c Western blot analyses of immunoprecipitated (IP) RhoA in MCF-10A cells treated DMSO (as control), 1 μM cerivastatin (STAT), 1 μM cerivastatin and 20 μM GGPP (STAT+GGPP), 5 μM GGTI-298 or 5 μM FTI-277. IgGs were used as IP control. d LDLR-Luc assay in MCF-10A cells 12 h after transfection with pcDNA3-GFP control plasmid, pcDNA3-GFP-RhoA G14V construct with a 6 h DMSO treatment, pcDNA3-GFP-RhoA G14V construct with a 6 h Latrunculin A (G14V + Lat.A) treatment, or pcDNA3-GFP-RhoA T19N construct. e LDLR-Luc assay in MCF-10A cells treated with either DMSO as control or C3 for 24 h. f LDLR-Luc assay in MCF-10A cells treated with either DMSO as control, Y-27632, or Blebbistatin (Blebbist.) for 24 h. g BODIPY 493/503 staining of lipid droplets (in red) in Mahlavu cells treated with either DMSO, C3 or Y-27632 for 24 h. Scale bar, 15 μm. h , i BODIPY 493/503 staining of lipid droplets (in red) and immunofluorescence analysis of dSREBP (in green) and phosphorylated myosin light chain (pMLC2, in magenta) in Drosophila larval fat body from h flies expressing either Luciferase or dRhoA RNAi , or dRhoA RNAi and treated with fatostatin (FTS) and i wild-type flies treated with either DMSO or Y-27632. Scale bar, 20 μm. Graphs bars in ( c – f ) represent mean ± s.d. of n = 3 biological replicates. Values in ( d – f ) are expressed as Relative Luminometer Units (RLU). Nuclei in ( h – i ) were stained with HOECHST (in blue). Blots and images are representative of n = 3 biological replicates. P value: * P
    Luciferase Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2761 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase reporter vector/product/Promega
    Average 99 stars, based on 2761 article reviews
    Price from $9.99 to $1999.99
    luciferase reporter vector - by Bioz Stars, 2021-01
    99/100 stars
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    The STAT5 Luciferase Reporter cell line is a stably transfected Ba F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT5 responsive promoter so
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    The SRE Leeporter Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the serum response element
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    N/A
    The CRELeeporter Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the cAMP response element CRE
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    RhoA and acto-myosin regulate the activity of hSREBP1 and dSREBP. a Screening of low density lipoprotein receptor promoter-luciferase activity in MDA-MB-231 cells transfected with constructs expressing firefly (LDLR-Luc) and renilla (Rluc) luciferase and either control siRNA (siCTL) or siRNAs targeting genes encoding geranylgeranyl pyrophosphate transferase I (GGTase1) protein substrates. b Western blot analysis of MDA-MB-231 and Mahlavu cells 48 h after transfection with siCTL or RhoA targeting siRNAs (siR#1 and siR#2). Hsp90 was used as loading control. mSREBP indicates mature protein. c Western blot analyses of immunoprecipitated (IP) RhoA in MCF-10A cells treated DMSO (as control), 1 μM cerivastatin (STAT), 1 μM cerivastatin and 20 μM GGPP (STAT+GGPP), 5 μM GGTI-298 or 5 μM FTI-277. IgGs were used as IP control. d LDLR-Luc assay in MCF-10A cells 12 h after transfection with pcDNA3-GFP control plasmid, pcDNA3-GFP-RhoA G14V construct with a 6 h DMSO treatment, pcDNA3-GFP-RhoA G14V construct with a 6 h Latrunculin A (G14V + Lat.A) treatment, or pcDNA3-GFP-RhoA T19N construct. e LDLR-Luc assay in MCF-10A cells treated with either DMSO as control or C3 for 24 h. f LDLR-Luc assay in MCF-10A cells treated with either DMSO as control, Y-27632, or Blebbistatin (Blebbist.) for 24 h. g BODIPY 493/503 staining of lipid droplets (in red) in Mahlavu cells treated with either DMSO, C3 or Y-27632 for 24 h. Scale bar, 15 μm. h , i BODIPY 493/503 staining of lipid droplets (in red) and immunofluorescence analysis of dSREBP (in green) and phosphorylated myosin light chain (pMLC2, in magenta) in Drosophila larval fat body from h flies expressing either Luciferase or dRhoA RNAi , or dRhoA RNAi and treated with fatostatin (FTS) and i wild-type flies treated with either DMSO or Y-27632. Scale bar, 20 μm. Graphs bars in ( c – f ) represent mean ± s.d. of n = 3 biological replicates. Values in ( d – f ) are expressed as Relative Luminometer Units (RLU). Nuclei in ( h – i ) were stained with HOECHST (in blue). Blots and images are representative of n = 3 biological replicates. P value: * P

    Journal: Nature Communications

    Article Title: Sterol regulatory element binding protein 1 couples mechanical cues and lipid metabolism

    doi: 10.1038/s41467-019-09152-7

    Figure Lengend Snippet: RhoA and acto-myosin regulate the activity of hSREBP1 and dSREBP. a Screening of low density lipoprotein receptor promoter-luciferase activity in MDA-MB-231 cells transfected with constructs expressing firefly (LDLR-Luc) and renilla (Rluc) luciferase and either control siRNA (siCTL) or siRNAs targeting genes encoding geranylgeranyl pyrophosphate transferase I (GGTase1) protein substrates. b Western blot analysis of MDA-MB-231 and Mahlavu cells 48 h after transfection with siCTL or RhoA targeting siRNAs (siR#1 and siR#2). Hsp90 was used as loading control. mSREBP indicates mature protein. c Western blot analyses of immunoprecipitated (IP) RhoA in MCF-10A cells treated DMSO (as control), 1 μM cerivastatin (STAT), 1 μM cerivastatin and 20 μM GGPP (STAT+GGPP), 5 μM GGTI-298 or 5 μM FTI-277. IgGs were used as IP control. d LDLR-Luc assay in MCF-10A cells 12 h after transfection with pcDNA3-GFP control plasmid, pcDNA3-GFP-RhoA G14V construct with a 6 h DMSO treatment, pcDNA3-GFP-RhoA G14V construct with a 6 h Latrunculin A (G14V + Lat.A) treatment, or pcDNA3-GFP-RhoA T19N construct. e LDLR-Luc assay in MCF-10A cells treated with either DMSO as control or C3 for 24 h. f LDLR-Luc assay in MCF-10A cells treated with either DMSO as control, Y-27632, or Blebbistatin (Blebbist.) for 24 h. g BODIPY 493/503 staining of lipid droplets (in red) in Mahlavu cells treated with either DMSO, C3 or Y-27632 for 24 h. Scale bar, 15 μm. h , i BODIPY 493/503 staining of lipid droplets (in red) and immunofluorescence analysis of dSREBP (in green) and phosphorylated myosin light chain (pMLC2, in magenta) in Drosophila larval fat body from h flies expressing either Luciferase or dRhoA RNAi , or dRhoA RNAi and treated with fatostatin (FTS) and i wild-type flies treated with either DMSO or Y-27632. Scale bar, 20 μm. Graphs bars in ( c – f ) represent mean ± s.d. of n = 3 biological replicates. Values in ( d – f ) are expressed as Relative Luminometer Units (RLU). Nuclei in ( h – i ) were stained with HOECHST (in blue). Blots and images are representative of n = 3 biological replicates. P value: * P

    Article Snippet: Forty-eight hours after transfection of the reporter (i.e. 72 h after siRNA transfection), the cells were lysed in 1 × Glo Lysis Buffer (Promega); Firefly and Renilla luciferase activities were measured using the Dual-Glo Luciferase Assay System (Promega), according to the manufacturer’s instructions, using an Envision Multimode Plate Reader (PerkinElmer).

    Techniques: Activity Assay, Luciferase, Multiple Displacement Amplification, Transfection, Construct, Expressing, Western Blot, Immunoprecipitation, Plasmid Preparation, Staining, Immunofluorescence

    Validation of DENV host factor genes (a) Plaque-forming units (PFU) assay of DENV infection. ND indicates no plaques were detected (threshold of detection of the assay is 6 PFU/ml). (b) DENV luciferase levels in HAP1 isogenic knockout cells complemented using lentiviral stable expression of corresponding genes. (c) Crystal violet of complemented Huh7 knockout cells infected with DENV. (d) DENV luciferase levels in Raji DC-SIGN cells with KO in DENV host factors (lentiCRISPRv2). Empty denotes an empty vector control (expressing Cas9 but no guideRNA) and NT a cell line expressing a non-targeting guideRNA. (e) Time course of DENV and HCV expressing Renilla luciferase in Huh7 knockout cells. (f) Schematic diagram of the STT3A and STT3B isoforms. Gene names in red indicate OST subunits identified in the DENV screens. Data depict average with s.d. for triplicate infections.

    Journal: Nature

    Article Title: Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens

    doi: 10.1038/nature18631

    Figure Lengend Snippet: Validation of DENV host factor genes (a) Plaque-forming units (PFU) assay of DENV infection. ND indicates no plaques were detected (threshold of detection of the assay is 6 PFU/ml). (b) DENV luciferase levels in HAP1 isogenic knockout cells complemented using lentiviral stable expression of corresponding genes. (c) Crystal violet of complemented Huh7 knockout cells infected with DENV. (d) DENV luciferase levels in Raji DC-SIGN cells with KO in DENV host factors (lentiCRISPRv2). Empty denotes an empty vector control (expressing Cas9 but no guideRNA) and NT a cell line expressing a non-targeting guideRNA. (e) Time course of DENV and HCV expressing Renilla luciferase in Huh7 knockout cells. (f) Schematic diagram of the STT3A and STT3B isoforms. Gene names in red indicate OST subunits identified in the DENV screens. Data depict average with s.d. for triplicate infections.

    Article Snippet: Luciferase expression was measured using Renilla Luciferase Assay system (Promega E2820).

    Techniques: Infection, Luciferase, Knock-Out, Expressing, Plasmid Preparation

    Physical interaction between the OST complex and the replication complex of DENV (a) APEX2, a protein tag for electron microscopy was fused to the C-terminus of STT3B enabling the imaging of subcellular protein localization by deposition of a polymer of 3,3′-diaminobenzidine (DAB). (b) Luminescence of Huh7 STT3B -KO cells complemented with STT3B-APEX2 and infected with DENV expressing Renilla luciferase. Data depict average with s.d. for triplicate infections. (c) STT3B localizes on ER membranes in the vicinity of DENV-induced vesicle packets as shown by transmission EM micrograph of DENV-infected or uninfected Huh7 cells expressing the STT3B-APEX2 construct. N represents the cell Nucleus and the arrowheads in samples transfected with STT3B-APEX2 represent APEX polymerized DAB staining in the lumen of the Endoplasmic Reticulum (ER) or around DENV-induced vesicle packets (VP). (d) Co-immunoprecipitations (IP) of STT3A-FLAG and STT3B-FLAG from DENV infected cell lysates. LE = long exposure. (e) Anti-FLAG Western blots of IP elutions of DENV infected cells stably expressing FLAG tagged STT3A, STT3B and RPS25. (f) SYPRO Ruby staining of elutions and inputs of IP of DENV infected cell lysates. (g) Co-IP elutions of DENV infected lysates were analyzed by mass spectrometry and DENV specific peptides aligned to DENV polyprotein.

    Journal: Nature

    Article Title: Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens

    doi: 10.1038/nature18631

    Figure Lengend Snippet: Physical interaction between the OST complex and the replication complex of DENV (a) APEX2, a protein tag for electron microscopy was fused to the C-terminus of STT3B enabling the imaging of subcellular protein localization by deposition of a polymer of 3,3′-diaminobenzidine (DAB). (b) Luminescence of Huh7 STT3B -KO cells complemented with STT3B-APEX2 and infected with DENV expressing Renilla luciferase. Data depict average with s.d. for triplicate infections. (c) STT3B localizes on ER membranes in the vicinity of DENV-induced vesicle packets as shown by transmission EM micrograph of DENV-infected or uninfected Huh7 cells expressing the STT3B-APEX2 construct. N represents the cell Nucleus and the arrowheads in samples transfected with STT3B-APEX2 represent APEX polymerized DAB staining in the lumen of the Endoplasmic Reticulum (ER) or around DENV-induced vesicle packets (VP). (d) Co-immunoprecipitations (IP) of STT3A-FLAG and STT3B-FLAG from DENV infected cell lysates. LE = long exposure. (e) Anti-FLAG Western blots of IP elutions of DENV infected cells stably expressing FLAG tagged STT3A, STT3B and RPS25. (f) SYPRO Ruby staining of elutions and inputs of IP of DENV infected cell lysates. (g) Co-IP elutions of DENV infected lysates were analyzed by mass spectrometry and DENV specific peptides aligned to DENV polyprotein.

    Article Snippet: Luciferase expression was measured using Renilla Luciferase Assay system (Promega E2820).

    Techniques: Electron Microscopy, Imaging, Infection, Expressing, Luciferase, Transmission Assay, Construct, Transfection, Staining, Western Blot, Stable Transfection, Co-Immunoprecipitation Assay, Mass Spectrometry