Journal: Journal of Toxicology
Article Title: Protective Effect of Liposome-Encapsulated Glutathione in a Human Epidermal Model Exposed to a Mustard Gas Analog
Figure Lengend Snippet: Protective effects of antioxidant liposomes in the EpiDerm model . EpiDerm tissues were exposed topically to 2.5 mM CEES or vehicle (1% DMSO) in the absence or presence of Blank liposomes (B-liposomes), NAC-liposomes, and GSH-liposomes (as indicated) applied simultaneously with CEES or vehicle, but into the bottom media; the tissues were incubated at 37°C for 18 hours. Final “calculated” concentration of GSH or NAC was 10 mM (see Section 2 ). Cell viability was monitored by the MTS assay (a). Cellular ATP was assayed in the tissue homogenates by the ATP assay (b). Means not sharing a common letter are significantly different ( P
Article Snippet: Materials and Reagents Stemline Keratinocyte Medium II (without L-glutamine, calcium-free), fluorimetric Caspase-3 Assay Kit, Sigma Luciferase ATP Determination kit, CEES, NAC, GSH, cholesterol, RRR-alpha-tocopherol, RRR-gamma-tocopherol, R-alpha-lipoic acid, DMSO, EDTA, and all organic solvents were obtained from Sigma Chemical Company (Sigma-Aldrich Inc., St. Louis, Mo).
Techniques: Incubation, Concentration Assay, MTS Assay, ATP Assay