Journal: Journal of Bacteriology
Article Title: Novel Phospholipase A Activity Secreted by Legionella Species
Figure Lengend Snippet: Apolar and polar lipids after incubation of PC with CCCS of L. pneumophila . PC was incubated with the CCCS of different L. pneumophila strains for 16 h. Apolar (A) and polar (B) lipids were characterized by TLC. A representative experiment is shown. Abbreviations: St, standard (1A, 1,2-dipalmitoylglycerol, palmitic acid; 1B, LPC, dipalmitoylphosphatidylcholine); C, negative control (concentrated BYE broth or concentrated −P broth, respectively); P, positive control (PLC from C. perfringens ); 1, Lp1-MM6; 2, Lp1-MRC; 3, Lp2-ATCC; 4, Lp6-c; 5, Lp12-ATCC.
Article Snippet: Next, we examined the hydrolysis of 3 H-PC by CCCS and by the 54-kDa AEC fraction of Lp6-c as described by Baine ( , ) at final concentrations of 20 and 18 μg of protein/ml, respectively.
Techniques: Incubation, Thin Layer Chromatography, Negative Control, Positive Control, Planar Chromatography