loopb1 primers Search Results


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  • 99
    New England Biolabs bst dna polymerase
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    Specificity of SFGR-LAMP assay using Bst 2.0 <t>WarmStart</t> <t>DNA</t> polymerase. (A). Colorimetric visual detection of hydroxynaphol blue-based SFGR-LAMP reaction products inspected by the naked eye. The color changes from light blue in the positive reactions to dark blue to purple in the negative reactions. (B). Agarose gel electrophoresis. Lane 1: 100Kbp molecular ladder; 2: water control; 3: positive control ( R . rickettsii ); 4: R . africae ; 5: R . amblyommatis ; 6: R . conorii ; 7: R . felis ; 8: R . montanensis ; 9: R . parkeri ; 10: R . rickettsii ; 11: R . typhi ; 12: E . chaffeensis ; 13: A . phagocytophilum ; 14: C . burnetii ; 15: E . coli ; 16: S . typhi .
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    Specificity of SFGR-LAMP assay using Bst 2.0 <t>WarmStart</t> <t>DNA</t> polymerase. (A). Colorimetric visual detection of hydroxynaphol blue-based SFGR-LAMP reaction products inspected by the naked eye. The color changes from light blue in the positive reactions to dark blue to purple in the negative reactions. (B). Agarose gel electrophoresis. Lane 1: 100Kbp molecular ladder; 2: water control; 3: positive control ( R . rickettsii ); 4: R . africae ; 5: R . amblyommatis ; 6: R . conorii ; 7: R . felis ; 8: R . montanensis ; 9: R . parkeri ; 10: R . rickettsii ; 11: R . typhi ; 12: E . chaffeensis ; 13: A . phagocytophilum ; 14: C . burnetii ; 15: E . coli ; 16: S . typhi .
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    Image Search Results


    Specificity of SFGR-LAMP assay using Bst 2.0 WarmStart DNA polymerase. (A). Colorimetric visual detection of hydroxynaphol blue-based SFGR-LAMP reaction products inspected by the naked eye. The color changes from light blue in the positive reactions to dark blue to purple in the negative reactions. (B). Agarose gel electrophoresis. Lane 1: 100Kbp molecular ladder; 2: water control; 3: positive control ( R . rickettsii ); 4: R . africae ; 5: R . amblyommatis ; 6: R . conorii ; 7: R . felis ; 8: R . montanensis ; 9: R . parkeri ; 10: R . rickettsii ; 11: R . typhi ; 12: E . chaffeensis ; 13: A . phagocytophilum ; 14: C . burnetii ; 15: E . coli ; 16: S . typhi .

    Journal: PLoS ONE

    Article Title: Development of a loop-mediated isothermal amplification (LAMP) assay for rapid screening of ticks and fleas for spotted fever group rickettsia

    doi: 10.1371/journal.pone.0192331

    Figure Lengend Snippet: Specificity of SFGR-LAMP assay using Bst 2.0 WarmStart DNA polymerase. (A). Colorimetric visual detection of hydroxynaphol blue-based SFGR-LAMP reaction products inspected by the naked eye. The color changes from light blue in the positive reactions to dark blue to purple in the negative reactions. (B). Agarose gel electrophoresis. Lane 1: 100Kbp molecular ladder; 2: water control; 3: positive control ( R . rickettsii ); 4: R . africae ; 5: R . amblyommatis ; 6: R . conorii ; 7: R . felis ; 8: R . montanensis ; 9: R . parkeri ; 10: R . rickettsii ; 11: R . typhi ; 12: E . chaffeensis ; 13: A . phagocytophilum ; 14: C . burnetii ; 15: E . coli ; 16: S . typhi .

    Article Snippet: Bst 2.0 LAMP Each LAMP reaction totaled 25 μl consisting of 9 μl nuclease free water, 2.5 μl 10x Isothermal Amplification Buffer Pack (contains 20mM Tris-HCl, 10mM (NH4 )2 SO4 , 50mM KCl, 2mM MgSO4 , 0.1% Tween® 20) (New England Biolabs), 3.5 μl 10 mM each dNTPs (New England Biolabs), 1 μl 100 mM MgSO4 (New England Biolabs), 3.5 μl 5M Betaine (Affymetrix), and 2.5 μl 10x primer mix (2μM F3 primer, 2μM B3 primer, 16μM FIP, 16μM BIP, 8μM LoopF and 8μM LoopB), 1.0 μl Bst 2.0 WarmStart DNA Polymerase (8 units/μl) (New England Biolabs), 1.0 μl 3mM Hydroxynaphthol blue (HNB) (Sigma-Aldrich), and 1 μl of sample DNA.

    Techniques: Lamp Assay, Agarose Gel Electrophoresis, Positive Control