liquid chromatography-tandem mass spectrometry Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore chromatography tandem mass spectrometry
    Flowchart for pilot neonatal congenital adrenal hyperplasia screening initiated by the Turkish Directorate of Public Health CAH: congenital adrenal hyperplasia, FIA: fluoroimmunoassay, 17-OHP: 17-hydroxyprogesterone, LC-MS/MS: <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> F: cortisol
    Chromatography Tandem Mass Spectrometry, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chromatography tandem mass spectrometry/product/Millipore
    Average 99 stars, based on 85 article reviews
    Price from $9.99 to $1999.99
    chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    93
    Waters Corporation liquid chromatography tandem mass spectrometry
    Exhaled breath (EB) samples tested by reversed‐phase <t>liquid</t> <t>chromatography/tandem</t> <t>mass</t> <t>spectrometry</t> for 12 model compounds including anabolic agents, hormone and metabolic modulators, stimulants, and beta‐blockers: (A) blank EB specimen containing only the internal standards D 3 ‐testosterone, D 3 ‐meldonium, ( S )‐2‐aminooctane, and D 7 ‐propranolol. Y‐axes are normalized to the abundance of the corresponding spiked specimen shown under (B), which illustrates the results of an EB sample fortified with 500 pg of each target analyte plus ISTDs
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 610 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/Waters Corporation
    Average 93 stars, based on 610 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    93
    SCIEX liquid chromatography tandem mass spectrometry
    Proteins that interact with RRAD. Notes: ( A ) Proteins that predicted to interact with RRAD on STRING (Szklarczyk D, Franceschini A, Wyder S, et al. STRING v10: protein-protein interaction networks, integrated over the tree of life. Nucleic Acids Res . 2015;43(Database issue):D447–D452). 35 ( B ) Expression of P53 after overexpression of RRAD and expression of RRAD after overexpression of P53 by Western blotting. ( C ) The Venn diagram of the number of proteins after Co-IP assays and LC-MS/MS analysis. ( D ) GST (glutathione-S-transferase) pull-down assay for ACTG1/EF1A1 with RRAD. Abbreviations: RRAD, Ras-related associated with diabetes; Co-IP, co-immunoprecipitation; LC-MS/MS, <t>liquid</t> <t>chromatography–tandem</t> <t>mass</t> <t>spectrometry;</t> ACTG1, actin gamma 1; NC, negative control.
    Liquid Chromatography Tandem Mass Spectrometry, supplied by SCIEX, used in various techniques. Bioz Stars score: 93/100, based on 594 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/SCIEX
    Average 93 stars, based on 594 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    ARUP Laboratories liquid chromatography tandem mass spectrometry
    Proteins that interact with RRAD. Notes: ( A ) Proteins that predicted to interact with RRAD on STRING (Szklarczyk D, Franceschini A, Wyder S, et al. STRING v10: protein-protein interaction networks, integrated over the tree of life. Nucleic Acids Res . 2015;43(Database issue):D447–D452). 35 ( B ) Expression of P53 after overexpression of RRAD and expression of RRAD after overexpression of P53 by Western blotting. ( C ) The Venn diagram of the number of proteins after Co-IP assays and LC-MS/MS analysis. ( D ) GST (glutathione-S-transferase) pull-down assay for ACTG1/EF1A1 with RRAD. Abbreviations: RRAD, Ras-related associated with diabetes; Co-IP, co-immunoprecipitation; LC-MS/MS, <t>liquid</t> <t>chromatography–tandem</t> <t>mass</t> <t>spectrometry;</t> ACTG1, actin gamma 1; NC, negative control.
    Liquid Chromatography Tandem Mass Spectrometry, supplied by ARUP Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/ARUP Laboratories
    Average 92 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    93
    Agilent technologies liquid chromatography tandem mass spectrometry
    Wld S downregulates UCP2 expression and upregulates ATP levels through SIRT1. A : Wld S colocalized with SIRT1 in MIN6 cells. The stable cell lines expressing EGFP-Wld S were transiently transfected with pCMV-myc-SIRT1 and stained with anti-Myc antibody and DAPI. Scale bar, 5 μm. B : Wld S was coimmunoprecipitated with SIRT1 from the pancreatic lysates of Wld S mice. C : Wld S and its enzyme-dead mutant Wld S -H112A coimmunoprecipitated with SIRT1. The MIN6 cells stably expressing EGFP, EGFP-Wld S , or EGFP-Wld S -H112A were used for immunoprecipitation. UCP2 protein levels were also detected by Western blot. D and E : <t>Liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry</t> analysis of NMN, NADP, NADPH, NAD, NADH, NA, and NAM extracted from pancreas of 9-week-old wild-type (WT) ( D ) or Wld S mice ( E ). *With significant difference. F : Quantification of the small molecules corresponding to D and E showed that NAD and NMN levels were upregulated in the pancreas of Wld S mice ( n = 4 for each genotype). G : Wld S repressed UCP2 promoter activity like SIRT1. UCP2 promoter activity was measured by luciferase assay in 293T cells transfected with pGL3-UCP2-Promoter and the indicated plasmids. H : Wld S downregulated UCP2 mRNA levels dependent on its enzyme activity. UCP2 mRNA level was measured by real-time PCR with MIN6 cell lines stably expressing EGFP, EGFP-Wld S , and EGFP-Wld S -H112A. I : Wld S downregulated UCP2 mRNA levels via SIRT1. UCP2 mRNA levels were determined by real-time PCR using islets isolated from mice with the indicated genotype ( n = 4 for each genotype). J : Wld S downregulated UCP2 protein levels via SIRT1. The protein levels in brown fat tissue with the indicated genotype were detected with SIRT1, Wld S , UCP2, and tubulin antibodies ( n = 3 for each genotype). K : Quantification of the UCP2 protein levels corresponding to J . L : Wld S increased ATP levels in primary cultured islets at the indicated glucose concentration ( n = 3). M : Wld S upregulated ATP level in islets via SIRT1. ATP levels were measured in islets with indicated genotypes at 2 mmol/L or 20 mmol/L glucose ( n = 3). * P
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 689 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/Agilent technologies
    Average 93 stars, based on 689 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    94
    Thermo Fisher liquid chromatography tandem mass spectrometry
    Absolute levels of tryptophan in urine samples. Tryptophan levels were measured from SLC22A2 reference ( n = 9), 808GT ( n = 6), and 808TT ( n = 6) carriers using selected reaction monitoring with <t>liquid</t> <t>chromatography-coupled</t> <t>tandem</t> <t>mass</t> <t>spectrometry.</t> The boundary of the box closest to zero indicates the 25 th percentile, a line within the box marks the median, and the boundary of the box farthest from zero indicates the 75 th percentile.
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/Thermo Fisher
    Average 94 stars, based on 2484 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    92
    Mayo Medical Laboratories liquid chromatography tandem mass spectrometry
    Absolute levels of tryptophan in urine samples. Tryptophan levels were measured from SLC22A2 reference ( n = 9), 808GT ( n = 6), and 808TT ( n = 6) carriers using selected reaction monitoring with <t>liquid</t> <t>chromatography-coupled</t> <t>tandem</t> <t>mass</t> <t>spectrometry.</t> The boundary of the box closest to zero indicates the 25 th percentile, a line within the box marks the median, and the boundary of the box farthest from zero indicates the 75 th percentile.
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Mayo Medical Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/Mayo Medical Laboratories
    Average 92 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Quest Diagnostics liquid chromatography tandem mass spectrometry
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Quest Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/Quest Diagnostics
    Average 92 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Maxxam Analytics liquid chromatography tandem mass spectrometry
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Maxxam Analytics, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/Maxxam Analytics
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Celerion liquid chromatography tandem mass spectrometry assay
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Liquid Chromatography Tandem Mass Spectrometry Assay, supplied by Celerion, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry assay/product/Celerion
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry assay - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    Thermo Fisher turboionspray liquid chromatography tandem mass spectrometry
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Turboionspray Liquid Chromatography Tandem Mass Spectrometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/turboionspray liquid chromatography tandem mass spectrometry/product/Thermo Fisher
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    turboionspray liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    93
    Cergentis tandem liquid chromatography mass spectrometry
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Tandem Liquid Chromatography Mass Spectrometry, supplied by Cergentis, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tandem liquid chromatography mass spectrometry/product/Cergentis
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    tandem liquid chromatography mass spectrometry - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    93
    Agilent technologies tandem liquid chromatography mass spectrometry
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Tandem Liquid Chromatography Mass Spectrometry, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tandem liquid chromatography mass spectrometry/product/Agilent technologies
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    tandem liquid chromatography mass spectrometry - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    90
    Biocrates liquid chromatography lc tandem mass spectrometry tandem mass spectrometry ms ms assay
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Liquid Chromatography Lc Tandem Mass Spectrometry Tandem Mass Spectrometry Ms Ms Assay, supplied by Biocrates, used in various techniques. Bioz Stars score: 90/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography lc tandem mass spectrometry tandem mass spectrometry ms ms assay/product/Biocrates
    Average 90 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography lc tandem mass spectrometry tandem mass spectrometry ms ms assay - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    93
    Abbott Laboratories liquid chromatography tandem mass spectrometry
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/Abbott Laboratories
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Quotient Bioresearch liquid chromatography tandem mass spectrometry
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Quotient Bioresearch, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/Quotient Bioresearch
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Thermo Fisher nano liquid chromatography tandem mass spectrometry
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Nano Liquid Chromatography Tandem Mass Spectrometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano liquid chromatography tandem mass spectrometry/product/Thermo Fisher
    Average 92 stars, based on 591 article reviews
    Price from $9.99 to $1999.99
    nano liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    ARUP Laboratories quantitative liquid chromatography tandem mass spectrometry
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Quantitative Liquid Chromatography Tandem Mass Spectrometry, supplied by ARUP Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative liquid chromatography tandem mass spectrometry/product/ARUP Laboratories
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    quantitative liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    92
    Shimadzu Corporation liquid chromatography tandem mass spectrometry analysis liquid chromatography
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Liquid Chromatography Tandem Mass Spectrometry Analysis Liquid Chromatography, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry analysis liquid chromatography/product/Shimadzu Corporation
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography tandem mass spectrometry analysis liquid chromatography - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Flowchart for pilot neonatal congenital adrenal hyperplasia screening initiated by the Turkish Directorate of Public Health CAH: congenital adrenal hyperplasia, FIA: fluoroimmunoassay, 17-OHP: 17-hydroxyprogesterone, LC-MS/MS: liquid chromatography-tandem mass spectrometry, F: cortisol

    Journal: Journal of Clinical Research in Pediatric Endocrinology

    Article Title: Neonatal Screening for Congenital Adrenal Hyperplasia in Turkey: A Pilot Study with 38,935 Infants

    doi: 10.4274/jcrpe.galenos.2018.2018.0117

    Figure Lengend Snippet: Flowchart for pilot neonatal congenital adrenal hyperplasia screening initiated by the Turkish Directorate of Public Health CAH: congenital adrenal hyperplasia, FIA: fluoroimmunoassay, 17-OHP: 17-hydroxyprogesterone, LC-MS/MS: liquid chromatography-tandem mass spectrometry, F: cortisol

    Article Snippet: Reagent, Instruments, and Analytical Conditions for Liquid Chromatography-tandem Mass Spectrometry The steroid standards for F, 17-OHP, 21-S, 11-S, 4AS and deuterated steroid standards for d4-F, d8-17-OHP, d3-testosterone were purchased from Sigma-Aldrich (MO, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Liquid Chromatography

    Exhaled breath (EB) samples tested by reversed‐phase liquid chromatography/tandem mass spectrometry for 12 model compounds including anabolic agents, hormone and metabolic modulators, stimulants, and beta‐blockers: (A) blank EB specimen containing only the internal standards D 3 ‐testosterone, D 3 ‐meldonium, ( S )‐2‐aminooctane, and D 7 ‐propranolol. Y‐axes are normalized to the abundance of the corresponding spiked specimen shown under (B), which illustrates the results of an EB sample fortified with 500 pg of each target analyte plus ISTDs

    Journal: Rapid Communications in Mass Spectrometry

    Article Title: Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath. Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath

    doi: 10.1002/rcm.7903

    Figure Lengend Snippet: Exhaled breath (EB) samples tested by reversed‐phase liquid chromatography/tandem mass spectrometry for 12 model compounds including anabolic agents, hormone and metabolic modulators, stimulants, and beta‐blockers: (A) blank EB specimen containing only the internal standards D 3 ‐testosterone, D 3 ‐meldonium, ( S )‐2‐aminooctane, and D 7 ‐propranolol. Y‐axes are normalized to the abundance of the corresponding spiked specimen shown under (B), which illustrates the results of an EB sample fortified with 500 pg of each target analyte plus ISTDs

    Article Snippet: 2.2 Liquid chromatography/tandem mass spectrometry (LC/MS/MS) All analyses were conducted using a Aquity I‐Class ultra‐performance liquid chromatography (UPLC) system (Waters, Eschborn, Germany) interfaced via unispray (US) ionization to a Xevo TQ‐XS tandem mass spectrometer (Waters).

    Techniques: Liquid Chromatography, Mass Spectrometry

    Proteins that interact with RRAD. Notes: ( A ) Proteins that predicted to interact with RRAD on STRING (Szklarczyk D, Franceschini A, Wyder S, et al. STRING v10: protein-protein interaction networks, integrated over the tree of life. Nucleic Acids Res . 2015;43(Database issue):D447–D452). 35 ( B ) Expression of P53 after overexpression of RRAD and expression of RRAD after overexpression of P53 by Western blotting. ( C ) The Venn diagram of the number of proteins after Co-IP assays and LC-MS/MS analysis. ( D ) GST (glutathione-S-transferase) pull-down assay for ACTG1/EF1A1 with RRAD. Abbreviations: RRAD, Ras-related associated with diabetes; Co-IP, co-immunoprecipitation; LC-MS/MS, liquid chromatography–tandem mass spectrometry; ACTG1, actin gamma 1; NC, negative control.

    Journal: OncoTargets and therapy

    Article Title: RRAD suppresses the Warburg effect by downregulating ACTG1 in hepatocellular carcinoma

    doi: 10.2147/OTT.S197844

    Figure Lengend Snippet: Proteins that interact with RRAD. Notes: ( A ) Proteins that predicted to interact with RRAD on STRING (Szklarczyk D, Franceschini A, Wyder S, et al. STRING v10: protein-protein interaction networks, integrated over the tree of life. Nucleic Acids Res . 2015;43(Database issue):D447–D452). 35 ( B ) Expression of P53 after overexpression of RRAD and expression of RRAD after overexpression of P53 by Western blotting. ( C ) The Venn diagram of the number of proteins after Co-IP assays and LC-MS/MS analysis. ( D ) GST (glutathione-S-transferase) pull-down assay for ACTG1/EF1A1 with RRAD. Abbreviations: RRAD, Ras-related associated with diabetes; Co-IP, co-immunoprecipitation; LC-MS/MS, liquid chromatography–tandem mass spectrometry; ACTG1, actin gamma 1; NC, negative control.

    Article Snippet: Analysis was performed using liquid chromatography–tandem mass spectrometry (LC-MS/MS; Ekspert NanoLC and TripleTOF 5600+; Sciex, Framingham, MA, USA).

    Techniques: Expressing, Over Expression, Western Blot, Co-Immunoprecipitation Assay, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Pull Down Assay, Immunoprecipitation, Liquid Chromatography, Negative Control

    Wld S downregulates UCP2 expression and upregulates ATP levels through SIRT1. A : Wld S colocalized with SIRT1 in MIN6 cells. The stable cell lines expressing EGFP-Wld S were transiently transfected with pCMV-myc-SIRT1 and stained with anti-Myc antibody and DAPI. Scale bar, 5 μm. B : Wld S was coimmunoprecipitated with SIRT1 from the pancreatic lysates of Wld S mice. C : Wld S and its enzyme-dead mutant Wld S -H112A coimmunoprecipitated with SIRT1. The MIN6 cells stably expressing EGFP, EGFP-Wld S , or EGFP-Wld S -H112A were used for immunoprecipitation. UCP2 protein levels were also detected by Western blot. D and E : Liquid chromatography-tandem mass spectrometry analysis of NMN, NADP, NADPH, NAD, NADH, NA, and NAM extracted from pancreas of 9-week-old wild-type (WT) ( D ) or Wld S mice ( E ). *With significant difference. F : Quantification of the small molecules corresponding to D and E showed that NAD and NMN levels were upregulated in the pancreas of Wld S mice ( n = 4 for each genotype). G : Wld S repressed UCP2 promoter activity like SIRT1. UCP2 promoter activity was measured by luciferase assay in 293T cells transfected with pGL3-UCP2-Promoter and the indicated plasmids. H : Wld S downregulated UCP2 mRNA levels dependent on its enzyme activity. UCP2 mRNA level was measured by real-time PCR with MIN6 cell lines stably expressing EGFP, EGFP-Wld S , and EGFP-Wld S -H112A. I : Wld S downregulated UCP2 mRNA levels via SIRT1. UCP2 mRNA levels were determined by real-time PCR using islets isolated from mice with the indicated genotype ( n = 4 for each genotype). J : Wld S downregulated UCP2 protein levels via SIRT1. The protein levels in brown fat tissue with the indicated genotype were detected with SIRT1, Wld S , UCP2, and tubulin antibodies ( n = 3 for each genotype). K : Quantification of the UCP2 protein levels corresponding to J . L : Wld S increased ATP levels in primary cultured islets at the indicated glucose concentration ( n = 3). M : Wld S upregulated ATP level in islets via SIRT1. ATP levels were measured in islets with indicated genotypes at 2 mmol/L or 20 mmol/L glucose ( n = 3). * P

    Journal: Diabetes

    Article Title: WldS Enhances Insulin Transcription and Secretion via a SIRT1-Dependent Pathway and Improves Glucose Homeostasis

    doi: 10.2337/db11-0232

    Figure Lengend Snippet: Wld S downregulates UCP2 expression and upregulates ATP levels through SIRT1. A : Wld S colocalized with SIRT1 in MIN6 cells. The stable cell lines expressing EGFP-Wld S were transiently transfected with pCMV-myc-SIRT1 and stained with anti-Myc antibody and DAPI. Scale bar, 5 μm. B : Wld S was coimmunoprecipitated with SIRT1 from the pancreatic lysates of Wld S mice. C : Wld S and its enzyme-dead mutant Wld S -H112A coimmunoprecipitated with SIRT1. The MIN6 cells stably expressing EGFP, EGFP-Wld S , or EGFP-Wld S -H112A were used for immunoprecipitation. UCP2 protein levels were also detected by Western blot. D and E : Liquid chromatography-tandem mass spectrometry analysis of NMN, NADP, NADPH, NAD, NADH, NA, and NAM extracted from pancreas of 9-week-old wild-type (WT) ( D ) or Wld S mice ( E ). *With significant difference. F : Quantification of the small molecules corresponding to D and E showed that NAD and NMN levels were upregulated in the pancreas of Wld S mice ( n = 4 for each genotype). G : Wld S repressed UCP2 promoter activity like SIRT1. UCP2 promoter activity was measured by luciferase assay in 293T cells transfected with pGL3-UCP2-Promoter and the indicated plasmids. H : Wld S downregulated UCP2 mRNA levels dependent on its enzyme activity. UCP2 mRNA level was measured by real-time PCR with MIN6 cell lines stably expressing EGFP, EGFP-Wld S , and EGFP-Wld S -H112A. I : Wld S downregulated UCP2 mRNA levels via SIRT1. UCP2 mRNA levels were determined by real-time PCR using islets isolated from mice with the indicated genotype ( n = 4 for each genotype). J : Wld S downregulated UCP2 protein levels via SIRT1. The protein levels in brown fat tissue with the indicated genotype were detected with SIRT1, Wld S , UCP2, and tubulin antibodies ( n = 3 for each genotype). K : Quantification of the UCP2 protein levels corresponding to J . L : Wld S increased ATP levels in primary cultured islets at the indicated glucose concentration ( n = 3). M : Wld S upregulated ATP level in islets via SIRT1. ATP levels were measured in islets with indicated genotypes at 2 mmol/L or 20 mmol/L glucose ( n = 3). * P

    Article Snippet: The samples were then analyzed using a liquid chromatography-tandem mass spectrometry with an Agilent 1200 series high-performance liquid chromatography system and a 4000 Q Trap tandem mass spectrometer (Applied Biosystems).

    Techniques: Expressing, Stable Transfection, Transfection, Staining, Mouse Assay, Mutagenesis, Immunoprecipitation, Western Blot, Liquid Chromatography, Mass Spectrometry, Activity Assay, Luciferase, Real-time Polymerase Chain Reaction, Isolation, Cell Culture, Concentration Assay

    Absolute levels of tryptophan in urine samples. Tryptophan levels were measured from SLC22A2 reference ( n = 9), 808GT ( n = 6), and 808TT ( n = 6) carriers using selected reaction monitoring with liquid chromatography-coupled tandem mass spectrometry. The boundary of the box closest to zero indicates the 25 th percentile, a line within the box marks the median, and the boundary of the box farthest from zero indicates the 75 th percentile.

    Journal: PLoS ONE

    Article Title: Pharmacogenetics Meets Metabolomics: Discovery of Tryptophan as a New Endogenous OCT2 Substrate Related to Metformin Disposition

    doi: 10.1371/journal.pone.0036637

    Figure Lengend Snippet: Absolute levels of tryptophan in urine samples. Tryptophan levels were measured from SLC22A2 reference ( n = 9), 808GT ( n = 6), and 808TT ( n = 6) carriers using selected reaction monitoring with liquid chromatography-coupled tandem mass spectrometry. The boundary of the box closest to zero indicates the 25 th percentile, a line within the box marks the median, and the boundary of the box farthest from zero indicates the 75 th percentile.

    Article Snippet: Assay of tryptophan concentrations using liquid chromatography-tandem mass spectrometry Tryptophan concentrations in urine samples were quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS; API 3000; Applied Biosystems, Foster City, CA).

    Techniques: Liquid Chromatography, Mass Spectrometry

    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - liquid chromatography-tandem mass spectrometry, HPLC - high performance liquid chromatography

    Journal: Saudi Medical Journal

    Article Title: Assessment of low vitamin D among Saudi Arabians

    doi:

    Figure Lengend Snippet: Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - liquid chromatography-tandem mass spectrometry, HPLC - high performance liquid chromatography

    Article Snippet: Liquid chromatography-tandem mass spectrometry The third method used to measure 25-OHD levels in this study was LC-MS/MS, which was carried out at the Quest Diagnostics, Lab One, Ohio, USA using an API 3000 triple-quadrupole mass spectrometer (Applied Biosystems/MDS Sciex, AB Framingham, MA, USA) following the guidelines by the US National Institute of Standards and Technology (NIST).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Chemiluminescence Immunoassay, Liquid Chromatography, High Performance Liquid Chromatography

    Bland-Altman plot for RIA-LCMS/MS. RIA - radio-immuno assay, LC-MS/MS - liquid chromatography-tandem mass spectrometry

    Journal: Saudi Medical Journal

    Article Title: Assessment of low vitamin D among Saudi Arabians

    doi:

    Figure Lengend Snippet: Bland-Altman plot for RIA-LCMS/MS. RIA - radio-immuno assay, LC-MS/MS - liquid chromatography-tandem mass spectrometry

    Article Snippet: Liquid chromatography-tandem mass spectrometry The third method used to measure 25-OHD levels in this study was LC-MS/MS, which was carried out at the Quest Diagnostics, Lab One, Ohio, USA using an API 3000 triple-quadrupole mass spectrometer (Applied Biosystems/MDS Sciex, AB Framingham, MA, USA) following the guidelines by the US National Institute of Standards and Technology (NIST).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, RIA Assay, Liquid Chromatography

    Inter assay relationship between CLIA, RIA, and LCMS/MS. CLIA - chemiluminescence immunoassay, RIA - radio-immuno assay, LC-MS/MS - liquid chromatography-tandem mass spectrometry, HPLC - high-pressure liquid chromatography

    Journal: Saudi Medical Journal

    Article Title: Assessment of low vitamin D among Saudi Arabians

    doi:

    Figure Lengend Snippet: Inter assay relationship between CLIA, RIA, and LCMS/MS. CLIA - chemiluminescence immunoassay, RIA - radio-immuno assay, LC-MS/MS - liquid chromatography-tandem mass spectrometry, HPLC - high-pressure liquid chromatography

    Article Snippet: Liquid chromatography-tandem mass spectrometry The third method used to measure 25-OHD levels in this study was LC-MS/MS, which was carried out at the Quest Diagnostics, Lab One, Ohio, USA using an API 3000 triple-quadrupole mass spectrometer (Applied Biosystems/MDS Sciex, AB Framingham, MA, USA) following the guidelines by the US National Institute of Standards and Technology (NIST).

    Techniques: Inter Assay, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Chemiluminescence Immunoassay, RIA Assay, Liquid Chromatography, High Performance Liquid Chromatography