lipofectamine stem transfection reagent Search Results


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  • 99
    Thermo Fisher lipofectamine stem transfection reagent
    Appropriateness for CRISPR-editing. <t>Transfection</t> efficiencies using <t>Lipofectamine</t> STEM and the Lonza 4D nucleofector across three different sites: JHU, ISMMS, UC.
    Lipofectamine Stem Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2318 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine stem transfection reagent/product/Thermo Fisher
    Average 99 stars, based on 2318 article reviews
    Price from $9.99 to $1999.99
    lipofectamine stem transfection reagent - by Bioz Stars, 2020-09
    99/100 stars
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    92
    GlobalStem lipofectamine stem transfection reagent
    Appropriateness for CRISPR-editing. <t>Transfection</t> efficiencies using <t>Lipofectamine</t> STEM and the Lonza 4D nucleofector across three different sites: JHU, ISMMS, UC.
    Lipofectamine Stem Transfection Reagent, supplied by GlobalStem, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine stem transfection reagent/product/GlobalStem
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    lipofectamine stem transfection reagent - by Bioz Stars, 2020-09
    92/100 stars
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    91
    promega lipofectamine stem transfection reagent
    Appropriateness for CRISPR-editing. <t>Transfection</t> efficiencies using <t>Lipofectamine</t> STEM and the Lonza 4D nucleofector across three different sites: JHU, ISMMS, UC.
    Lipofectamine Stem Transfection Reagent, supplied by promega, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine stem transfection reagent/product/promega
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipofectamine stem transfection reagent - by Bioz Stars, 2020-09
    91/100 stars
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    91
    santa cruz biotechnology lipofectamine stem transfection reagent
    Appropriateness for CRISPR-editing. <t>Transfection</t> efficiencies using <t>Lipofectamine</t> STEM and the Lonza 4D nucleofector across three different sites: JHU, ISMMS, UC.
    Lipofectamine Stem Transfection Reagent, supplied by santa cruz biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine stem transfection reagent/product/santa cruz biotechnology
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipofectamine stem transfection reagent - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    99
    Thermo Fisher sirna transfection lipofectamine stem reagent
    Appropriateness for CRISPR-editing. <t>Transfection</t> efficiencies using <t>Lipofectamine</t> STEM and the Lonza 4D nucleofector across three different sites: JHU, ISMMS, UC.
    Sirna Transfection Lipofectamine Stem Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirna transfection lipofectamine stem reagent/product/Thermo Fisher
    Average 99 stars, based on 5 article reviews
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    sirna transfection lipofectamine stem reagent - by Bioz Stars, 2020-09
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    84
    origene using lipofectamine stem transfection reagent
    Appropriateness for CRISPR-editing. <t>Transfection</t> efficiencies using <t>Lipofectamine</t> STEM and the Lonza 4D nucleofector across three different sites: JHU, ISMMS, UC.
    Using Lipofectamine Stem Transfection Reagent, supplied by origene, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Appropriateness for CRISPR-editing. Transfection efficiencies using Lipofectamine STEM and the Lonza 4D nucleofector across three different sites: JHU, ISMMS, UC.

    Journal: bioRxiv

    Article Title: Publicly available hiPSC lines with extreme polygenic risk scores for modeling schizophrenia

    doi: 10.1101/2020.07.04.185348

    Figure Lengend Snippet: Appropriateness for CRISPR-editing. Transfection efficiencies using Lipofectamine STEM and the Lonza 4D nucleofector across three different sites: JHU, ISMMS, UC.

    Article Snippet: Twenty-four hours after plating cells each well is treated with 1.2 μl Lipofectamine STEM reagent (ThermoFisher, #STEM00001) and 500 ng pmaxGFP (Lonza, PBP3-00675) combined into 50μl Opti-MEM media following Lipofectamine STEM reagent protocol.

    Techniques: CRISPR, Transfection

    Smad3 regulates neuropilin 2 (NRP2) expression in cultured smooth muscle cells (SMCs). A–B , Smad3 loss‐of‐function reduces NRP2 expression levels. C–D , Smad3 gain‐of‐function increases NRP2 expression levels. Human primary aortic SMCs were transfected with scrambled small interfering RNA, Smad3‐specific small interfering RNA, empty vector, or Smad3 overexpression plasmid for 12 hours in basal medium (no fetal bovine serum). The cells were cultured for another 12 hours in fresh basal medium (no Lipofectamine) to recover, and then treated with 10 ng/mL of transforming growth factor β for 20 hours before harvest for Western blot and quantitative real‐time polymerase chain reaction (qRT‐PCR) analyses. Quantification: densitometry of Western blots (similar enhanced chemiluminescent exposure) from independent repeat experiments was normalized (to GAPDH) and then averaged to calculate mean±SEM (n=3). Readings of triplicate qRT‐PCR reactions were normalized (to GAPDH) and averaged to calculate mean±SD (n=3). Statistics: 1‐way ANOVA followed by Bonferroni post hoc test; *** P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Smad3 Regulates Neuropilin 2 Transcription by Binding to its 5′ Untranslated Region

    doi: 10.1161/JAHA.119.015487

    Figure Lengend Snippet: Smad3 regulates neuropilin 2 (NRP2) expression in cultured smooth muscle cells (SMCs). A–B , Smad3 loss‐of‐function reduces NRP2 expression levels. C–D , Smad3 gain‐of‐function increases NRP2 expression levels. Human primary aortic SMCs were transfected with scrambled small interfering RNA, Smad3‐specific small interfering RNA, empty vector, or Smad3 overexpression plasmid for 12 hours in basal medium (no fetal bovine serum). The cells were cultured for another 12 hours in fresh basal medium (no Lipofectamine) to recover, and then treated with 10 ng/mL of transforming growth factor β for 20 hours before harvest for Western blot and quantitative real‐time polymerase chain reaction (qRT‐PCR) analyses. Quantification: densitometry of Western blots (similar enhanced chemiluminescent exposure) from independent repeat experiments was normalized (to GAPDH) and then averaged to calculate mean±SEM (n=3). Readings of triplicate qRT‐PCR reactions were normalized (to GAPDH) and averaged to calculate mean±SD (n=3). Statistics: 1‐way ANOVA followed by Bonferroni post hoc test; *** P

    Article Snippet: In brief, 24 hours after transfection with luciferase assay plasmid, 5000 AoSMC cells per well were seeded in white 96‐well plates and cultured for 12 hours (no Lipofectamine).

    Techniques: Expressing, Cell Culture, Transfection, Small Interfering RNA, Plasmid Preparation, Over Expression, Western Blot, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    Cell viability and silencing efficiency depend on transfection conditions. a Scheme of the optimal gene silencing experiment: plating of cells (4.5 × 10 5 cells/well) and start of transfection at 0 h. Transfection complexes remained in the growth medium for 24 h until the medium exchange that was repeated at 48 h. At 48 h and 72 h time points, OCT4 level was analyzed by flow cytometry. Symbols: red line—transfection, black triangle—plating cells and adding transfection complexes, gray triangle—flow cytometric analysis, white triangle—medium exchange. b Flow cytometric analysis of OCT4 expression at 72 h in untreated cells or cells treated with 30 nM siOCT4 or siCtrl presented as percentage of OCT4-positive cells. X-axis represents the number of the cells plated into a well of 6-well plate. c Cell count normalized to untreated sample at 72 h time point while using optimal protocol. Abbreviations: UT, untreated. Statistical significance with P values less than 0.05 are labeled as * (mean ± SEM, N = 3)

    Journal: Stem Cell Research & Therapy

    Article Title: Targeted gene silencing in human embryonic stem cells using cell-penetrating peptide PepFect 14

    doi: 10.1186/s13287-019-1144-x

    Figure Lengend Snippet: Cell viability and silencing efficiency depend on transfection conditions. a Scheme of the optimal gene silencing experiment: plating of cells (4.5 × 10 5 cells/well) and start of transfection at 0 h. Transfection complexes remained in the growth medium for 24 h until the medium exchange that was repeated at 48 h. At 48 h and 72 h time points, OCT4 level was analyzed by flow cytometry. Symbols: red line—transfection, black triangle—plating cells and adding transfection complexes, gray triangle—flow cytometric analysis, white triangle—medium exchange. b Flow cytometric analysis of OCT4 expression at 72 h in untreated cells or cells treated with 30 nM siOCT4 or siCtrl presented as percentage of OCT4-positive cells. X-axis represents the number of the cells plated into a well of 6-well plate. c Cell count normalized to untreated sample at 72 h time point while using optimal protocol. Abbreviations: UT, untreated. Statistical significance with P values less than 0.05 are labeled as * (mean ± SEM, N = 3)

    Article Snippet: Transfection complexes of Lipofectamine Stem reagent (ThermoFisher) and siRNA (final concentration of 20 nM) were prepared according to manufacturer’s instructions.

    Techniques: Transfection, Flow Cytometry, Cytometry, Expressing, Cell Counting, Labeling

    PF14 delivers fluorescently labeled siRNA into hES cells with high efficiency. a Scheme of experiment: plating of cells (4.5 × 10 5 cells/well) and start of transfection at 0 h. Transfection complexes remained in the growth medium for 24 h. During that period, 3 independent flow cytometry analyses were performed (at 4 h, 8 h, and 24 h). After 24 h, the medium was changed for one without complexes and flow cytometric analysis was performed at 32 h. siRNA was used at concentration of 30 nM. Symbols: red line—transfection, black triangle—plating cells and adding transfection complexes, gray triangle—flow cytometric analysis, white triangle—medium exchange. b Flow cytometric analysis of siRNA-Alexa Fluor 568 in hES cells at various time points. Cells incubated with siCtrl/PF14 complexes were used as controls. Bars and numbers on histogram indicate the percentage of transfected cells in siRNA-Alexa Fluor 568-treated sample. c Immunofluorescence analysis of siRNA-Alexa Fluor 568 (red) localization in siRNA/PF14-treated cells after 24-h incubation. Cell nuclei were stained with DAPI (blue) and actin cytoskeleton with phalloidin (green). Scale bar is 20 μm

    Journal: Stem Cell Research & Therapy

    Article Title: Targeted gene silencing in human embryonic stem cells using cell-penetrating peptide PepFect 14

    doi: 10.1186/s13287-019-1144-x

    Figure Lengend Snippet: PF14 delivers fluorescently labeled siRNA into hES cells with high efficiency. a Scheme of experiment: plating of cells (4.5 × 10 5 cells/well) and start of transfection at 0 h. Transfection complexes remained in the growth medium for 24 h. During that period, 3 independent flow cytometry analyses were performed (at 4 h, 8 h, and 24 h). After 24 h, the medium was changed for one without complexes and flow cytometric analysis was performed at 32 h. siRNA was used at concentration of 30 nM. Symbols: red line—transfection, black triangle—plating cells and adding transfection complexes, gray triangle—flow cytometric analysis, white triangle—medium exchange. b Flow cytometric analysis of siRNA-Alexa Fluor 568 in hES cells at various time points. Cells incubated with siCtrl/PF14 complexes were used as controls. Bars and numbers on histogram indicate the percentage of transfected cells in siRNA-Alexa Fluor 568-treated sample. c Immunofluorescence analysis of siRNA-Alexa Fluor 568 (red) localization in siRNA/PF14-treated cells after 24-h incubation. Cell nuclei were stained with DAPI (blue) and actin cytoskeleton with phalloidin (green). Scale bar is 20 μm

    Article Snippet: Transfection complexes of Lipofectamine Stem reagent (ThermoFisher) and siRNA (final concentration of 20 nM) were prepared according to manufacturer’s instructions.

    Techniques: Labeling, Transfection, Flow Cytometry, Cytometry, Concentration Assay, Incubation, Immunofluorescence, Staining