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    Thermo Fisher lipofectamine ltx
    Cellular reprogramming with single lipofection and monolayer cardiac differentiation of iPSCs. (A): Outline of reprogramming of skin fibroblasts into iPSCs using lipofection with <t>Lipofectamine</t> <t>LTX</t> in 24-well plate scale. (B): The number of iPSC-like colonies
    Lipofectamine Ltx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 30728 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine ltx/product/Thermo Fisher
    Average 99 stars, based on 30728 article reviews
    Price from $9.99 to $1999.99
    lipofectamine ltx - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    90
    Fisher Scientific lipofectamine ltx
    a–d Luciferase Reporter Gene Assays with Caco-2 cells, applying Protocol #3. Caco-2 cells were transiently co-transfected with a human full-length LXRα ( a ) or LXRβ ( b ) expression plasmid, the firefly luciferase reporter plasmid ABCA1_Luc and an EGFP expression plasmid, in a ratio of 1:3:1, using <t>Lipofectamine™</t> <t>LTX.</t> In the case of the mammalian one-hybrid assays, cells were transiently transfected with an expression plasmid encoding a chimeric protein consisting of the yeast Gal4-DBD fused in frame to the hLXRα-LBD ( c ) or to the hLXRβ-LBD ( d ), a reporter plasmid containing specific upstream activating sequences (UAS)_Luc, and the EGFP expression plasmid, in a ratio of 5:3:1. Cells were then treated with GW3965 (LXR agonist, 0.1–10 μM) or the vehicle control (DMSO 0.1%). The luciferase-derived luminescence was normalized to the EGFP-derived fluorescence, and set in relation to the solvent vehicle. Bar graphs represent mean ± SD from three independent experiments each performed in quadruplicate. ns not significant, * P
    Lipofectamine Ltx, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine ltx/product/Fisher Scientific
    Average 90 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    lipofectamine ltx - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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    Cellular reprogramming with single lipofection and monolayer cardiac differentiation of iPSCs. (A): Outline of reprogramming of skin fibroblasts into iPSCs using lipofection with Lipofectamine LTX in 24-well plate scale. (B): The number of iPSC-like colonies

    Journal: Stem Cells Translational Medicine

    Article Title: Dual Optical Recordings for Action Potentials and Calcium Handling in Induced Pluripotent Stem Cell Models of Cardiac Arrhythmias Using Genetically Encoded Fluorescent Indicators

    doi: 10.5966/sctm.2014-0245

    Figure Lengend Snippet: Cellular reprogramming with single lipofection and monolayer cardiac differentiation of iPSCs. (A): Outline of reprogramming of skin fibroblasts into iPSCs using lipofection with Lipofectamine LTX in 24-well plate scale. (B): The number of iPSC-like colonies

    Article Snippet: Procedures using Xfect (Clontech, TaKaRa Bio, Shiga, Japan, ), Lipofectamine 2000, and Lipofectamine LTX (both from Life Technologies) were followed according to the manufacturer’s instructions. pGW1-YFP [ ] and pCXLE-enhanced green fluorescent protein (EGFP) (catalog no. 27082; Addgene, Cambridge, MA, ) were used to examine transfection efficiency.

    Techniques:

    Induction of apoptosis in MCF-7 cells by recombinant hemagglutinin-neuraminidase (HN) gene. MCF-7 cells were transfected with various amounts of recombinant of pDisplay/HN and cells were analyzed using flow cytometry at 48 h post transfection. Apoptosis induction was found to be dose-dependent. MCF-7 cells were also transfected with LTX reagent only and with pDisplay vector only. NTC, non-transfected MCF-7 cells. LTX, Lipofectamine ® LTX transfectant reagent. VEC, transfection with vector only. rHN, recombinant pDisplay/HN. Each bar represents the results of 3 independent experiments. Error bars indicate the standard of deviation.

    Journal: Oncology Reports

    Article Title: Induction of apoptosis in MCF-7 cells by the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus Malaysian strain AF2240

    doi: 10.3892/or.2013.2573

    Figure Lengend Snippet: Induction of apoptosis in MCF-7 cells by recombinant hemagglutinin-neuraminidase (HN) gene. MCF-7 cells were transfected with various amounts of recombinant of pDisplay/HN and cells were analyzed using flow cytometry at 48 h post transfection. Apoptosis induction was found to be dose-dependent. MCF-7 cells were also transfected with LTX reagent only and with pDisplay vector only. NTC, non-transfected MCF-7 cells. LTX, Lipofectamine ® LTX transfectant reagent. VEC, transfection with vector only. rHN, recombinant pDisplay/HN. Each bar represents the results of 3 independent experiments. Error bars indicate the standard of deviation.

    Article Snippet: For each transfection, 0.3–1.2 μg of the recombinant pDisplay-HN diluted in 100 μl of Opti-MEM® I reduced serum medium was transfected into MCF-7 cells using 1.25–10 μl of Lipofectamine® LTX Reagent according to the manufacturer’s instructions (both from Invitrogen Life Technologies).

    Techniques: Recombinant, Transfection, Flow Cytometry, Cytometry, Plasmid Preparation, Standard Deviation

    Characterization of commercial vectors (i.e., jetPRIME, GeneIn and Lipofectamine LTX/PLUS) in terms of size and charge. A) Size analysis of nanoparticles formed through complexation of each vector with 0.5 µg of pEGFP. The nanoparticles were formed following manufacturers’ protocols. B) Surface charge (Zeta potential) analysis of the same nanoparticles.

    Journal: Acta biomaterialia

    Article Title: Evaluation of Genotoxicity and Mutagenic Effects of Vector/DNA Nanocomplexes in Transfected Mesenchymal Stem Cells by Flow Cytometry

    doi: 10.1016/j.actbio.2018.05.029

    Figure Lengend Snippet: Characterization of commercial vectors (i.e., jetPRIME, GeneIn and Lipofectamine LTX/PLUS) in terms of size and charge. A) Size analysis of nanoparticles formed through complexation of each vector with 0.5 µg of pEGFP. The nanoparticles were formed following manufacturers’ protocols. B) Surface charge (Zeta potential) analysis of the same nanoparticles.

    Article Snippet: Gene transfer reagents, including GeneIn™ (AMSBIO, CA), Lipofectamine® LTX/PLUS™ (Thermo Fisher Scientific Inc.), and jetPRIME® (Polyplus-transfection® SA), were purchased from commercially available sources.

    Techniques: Plasmid Preparation

    CD8 + T cell response to 13 T . lestoquardi antigens measured by IFNγ ELISA. Sheep 4247 fibroblasts were transfected with expression constructs of candidate antigen genes using either Lipofectamine LTX or Fugene transfection reagents for 48 h before the addition of effector cells for 72 h and IFNγ ELISA. The antigens were tested separately and data are presented as mean ± SD of biological repeats (n = 3).

    Journal: PLoS ONE

    Article Title: Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells

    doi: 10.1371/journal.pone.0162571

    Figure Lengend Snippet: CD8 + T cell response to 13 T . lestoquardi antigens measured by IFNγ ELISA. Sheep 4247 fibroblasts were transfected with expression constructs of candidate antigen genes using either Lipofectamine LTX or Fugene transfection reagents for 48 h before the addition of effector cells for 72 h and IFNγ ELISA. The antigens were tested separately and data are presented as mean ± SD of biological repeats (n = 3).

    Article Snippet: Transfection was carried out either with Lipofectamine LTX & Plus Reagent (Invitrogen) or Fugene (Promega) at reagent (μl): DNA (μg) ratios of 5:1 and 3:1, respectively.

    Techniques: Enzyme-linked Immunosorbent Assay, Transfection, Expressing, Construct

    Knockdown efficacy evaluation of firefly luciferase by reverse transcription-polymerase chain reaction (RT-PCR). (A) Agarose gel electrophoresis of RT-PCR products. 0, no transfection control; M, Lipofectamine™ LTX mock control; B, empty vector pIRES2-EGFP control; 221, pIRES2-EGFP/miR-221-luc co-transfected sample (B) Quantitative analysis of RT-PCR result. Blank, empty vector pIRES2-EGFP control; miR-221-luc, pIRES2-EGFP/miR-221-luc co-transfected sample. Value represents the mean value of two experiments with standard deviation (SD).

    Journal: Experimental and Therapeutic Medicine

    Article Title: Construction of HCC-targeting artificial miRNAs using natural miRNA precursors

    doi: 10.3892/etm.2013.1111

    Figure Lengend Snippet: Knockdown efficacy evaluation of firefly luciferase by reverse transcription-polymerase chain reaction (RT-PCR). (A) Agarose gel electrophoresis of RT-PCR products. 0, no transfection control; M, Lipofectamine™ LTX mock control; B, empty vector pIRES2-EGFP control; 221, pIRES2-EGFP/miR-221-luc co-transfected sample (B) Quantitative analysis of RT-PCR result. Blank, empty vector pIRES2-EGFP control; miR-221-luc, pIRES2-EGFP/miR-221-luc co-transfected sample. Value represents the mean value of two experiments with standard deviation (SD).

    Article Snippet: The amiRNA precursor-expressing vectors or empty control vector (pIRES2-EGFP plasmids) and a typical 100 μ l transfection mixture was prepared with 1.5 μ g plasmid DNA (pIRES2-EGFP: pGL3.0-basic/TK: pGL4.74[hRluc /TK] in a 1:5:0.05 ratio) and 3 μ l transfection reagent Lipofectamine LTX (Invitrogen Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Techniques: Luciferase, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Transfection, Plasmid Preparation, Quantitative RT-PCR, Standard Deviation

    ) and chimera-targeting siRNA are color coded. (Top, left) MCF-7; PRKAA-TTC33 (cyan), SAMM50-PARVB (green), control (black). (Top, right) HBL-100; CHD2-CHMP1A (orange), control (black). (Bottom, left) OVCAR-3; URB1-C21ORF45 (blue), CTBS-GNG5 (red). (Bottom, right) HBL-100 treated with siRNA for chimeras from ovarian libraries; URB1-C21ORF45 (blue), CTBS-GNG5 (red). Bars, SD. Brackets, P value of two-way analysis of variance; Bonferroni t test significance: * P ≤ .05; *** P ≤ .001. (B) Real-time PCR of siRNA-transfected cells. (Top) Chimeric RNA (left to right: PRKAA-TTC33 and SAMM50-PARVB in MCF-7; URB1-C21ORF45 and CTBS-GNG5 in OVCAR-3). (Bottom) Single-partner RNA expression after the indicated siRNA treatment (left to right: PRKAA-TTC33 and SAMM50-PARVB in MCF-7). (C) Flow cytometry analysis of transfected HBL-100 and MCF7 cells; YFP was used as a transfection efficiency benchmark. (Left) HBL-100; LTX (red) or Lipo-2000 (blue). (Right) MCF-7; LTX transfection. (D) MCF-7 cell growth blockade after PRKAA-TTC33 -targeted or SAMM50-PARVB -targeted siRNA treatment (day 6 after transfection).

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Long-range Transcriptome Sequencing Reveals Cancer Cell Growth Regulatory Chimeric mRNA 1Long-range Transcriptome Sequencing Reveals Cancer Cell Growth Regulatory Chimeric mRNA 1 2

    doi:

    Figure Lengend Snippet: ) and chimera-targeting siRNA are color coded. (Top, left) MCF-7; PRKAA-TTC33 (cyan), SAMM50-PARVB (green), control (black). (Top, right) HBL-100; CHD2-CHMP1A (orange), control (black). (Bottom, left) OVCAR-3; URB1-C21ORF45 (blue), CTBS-GNG5 (red). (Bottom, right) HBL-100 treated with siRNA for chimeras from ovarian libraries; URB1-C21ORF45 (blue), CTBS-GNG5 (red). Bars, SD. Brackets, P value of two-way analysis of variance; Bonferroni t test significance: * P ≤ .05; *** P ≤ .001. (B) Real-time PCR of siRNA-transfected cells. (Top) Chimeric RNA (left to right: PRKAA-TTC33 and SAMM50-PARVB in MCF-7; URB1-C21ORF45 and CTBS-GNG5 in OVCAR-3). (Bottom) Single-partner RNA expression after the indicated siRNA treatment (left to right: PRKAA-TTC33 and SAMM50-PARVB in MCF-7). (C) Flow cytometry analysis of transfected HBL-100 and MCF7 cells; YFP was used as a transfection efficiency benchmark. (Left) HBL-100; LTX (red) or Lipo-2000 (blue). (Right) MCF-7; LTX transfection. (D) MCF-7 cell growth blockade after PRKAA-TTC33 -targeted or SAMM50-PARVB -targeted siRNA treatment (day 6 after transfection).

    Article Snippet: Cells were transfected with DNA in Lipofectamine 2000 (HBL-100, SKOV-3, IGROV-1, and OVCAR-3 cells) or LTX (Invitrogen, San Diego, CA), which was found to be optimal for MCF-7 cells ( ) [ ], following the manufacturer's instructions. pEYFP transfection was used to quantify transfection efficiency [ ] (EYFP expression, as measured by flow cytometry).

    Techniques: Real-time Polymerase Chain Reaction, Transfection, RNA Expression, Flow Cytometry, Cytometry

    a–d Luciferase Reporter Gene Assays with Caco-2 cells, applying Protocol #3. Caco-2 cells were transiently co-transfected with a human full-length LXRα ( a ) or LXRβ ( b ) expression plasmid, the firefly luciferase reporter plasmid ABCA1_Luc and an EGFP expression plasmid, in a ratio of 1:3:1, using Lipofectamine™ LTX. In the case of the mammalian one-hybrid assays, cells were transiently transfected with an expression plasmid encoding a chimeric protein consisting of the yeast Gal4-DBD fused in frame to the hLXRα-LBD ( c ) or to the hLXRβ-LBD ( d ), a reporter plasmid containing specific upstream activating sequences (UAS)_Luc, and the EGFP expression plasmid, in a ratio of 5:3:1. Cells were then treated with GW3965 (LXR agonist, 0.1–10 μM) or the vehicle control (DMSO 0.1%). The luciferase-derived luminescence was normalized to the EGFP-derived fluorescence, and set in relation to the solvent vehicle. Bar graphs represent mean ± SD from three independent experiments each performed in quadruplicate. ns not significant, * P

    Journal: Biological Procedures Online

    Article Title: Caco-2 Cells for Measuring Intestinal Cholesterol Transport - Possibilities and Limitations

    doi: 10.1186/s12575-020-00120-w

    Figure Lengend Snippet: a–d Luciferase Reporter Gene Assays with Caco-2 cells, applying Protocol #3. Caco-2 cells were transiently co-transfected with a human full-length LXRα ( a ) or LXRβ ( b ) expression plasmid, the firefly luciferase reporter plasmid ABCA1_Luc and an EGFP expression plasmid, in a ratio of 1:3:1, using Lipofectamine™ LTX. In the case of the mammalian one-hybrid assays, cells were transiently transfected with an expression plasmid encoding a chimeric protein consisting of the yeast Gal4-DBD fused in frame to the hLXRα-LBD ( c ) or to the hLXRβ-LBD ( d ), a reporter plasmid containing specific upstream activating sequences (UAS)_Luc, and the EGFP expression plasmid, in a ratio of 5:3:1. Cells were then treated with GW3965 (LXR agonist, 0.1–10 μM) or the vehicle control (DMSO 0.1%). The luciferase-derived luminescence was normalized to the EGFP-derived fluorescence, and set in relation to the solvent vehicle. Bar graphs represent mean ± SD from three independent experiments each performed in quadruplicate. ns not significant, * P

    Article Snippet: Lipofectamine™ LTX with PLUS™ reagent was purchased from Fisher Scientific (Vienna, Austria).

    Techniques: Luciferase, Transfection, Expressing, Plasmid Preparation, Derivative Assay, Fluorescence