lipofectamine 2000 reagent Search Results


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  • 99
    Thermo Fisher lipofectamine 2000 reagent
    Effects of dominant negative form of monomeric GTP-binding proteins on the induction of the promoter activity of IL-6 by H. pylori infection. MKN28 cells were cotransfected with 0.5 μg of the p1168hu.IL6P-luc+ plasmid together with 0.3 μg of one of the four plasmids, pCMV- RhoN19 , pCMV- RacN17 , pCMV- RasN17 , or pCMV plus 10 ng of Renilla plasmid as an internal control using <t>LipofectAMINE.</t> The transfected cells were subsequently infected with H. pylori . MKN28 cells were also cotransfected with the p1168hu.IL6P-luc+ plasmid together with either 50 ng of pCMV or pCMV-RafS621A plus 10 ng of Renilla plasmid and subsequently infected with H. pylori . Untreated plates served as controls. Shown is the normalized luciferase activity expressed as fold increase of luciferase activity in H. pylori -infected cells relative to uninfected controls. Five independent transfections, each run in triplicate, were performed. Data are expressed as mean ± SE; ** p
    Lipofectamine 2000 Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 73517 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 2000 reagent/product/Thermo Fisher
    Average 99 stars, based on 73517 article reviews
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    92
    Millipore lipofectamine 2000 reagent
    Effects of dominant negative form of monomeric GTP-binding proteins on the induction of the promoter activity of IL-6 by H. pylori infection. MKN28 cells were cotransfected with 0.5 μg of the p1168hu.IL6P-luc+ plasmid together with 0.3 μg of one of the four plasmids, pCMV- RhoN19 , pCMV- RacN17 , pCMV- RasN17 , or pCMV plus 10 ng of Renilla plasmid as an internal control using <t>LipofectAMINE.</t> The transfected cells were subsequently infected with H. pylori . MKN28 cells were also cotransfected with the p1168hu.IL6P-luc+ plasmid together with either 50 ng of pCMV or pCMV-RafS621A plus 10 ng of Renilla plasmid and subsequently infected with H. pylori . Untreated plates served as controls. Shown is the normalized luciferase activity expressed as fold increase of luciferase activity in H. pylori -infected cells relative to uninfected controls. Five independent transfections, each run in triplicate, were performed. Data are expressed as mean ± SE; ** p
    Lipofectamine 2000 Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 2000 reagent/product/Millipore
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    lipofectamine 2000 reagent - by Bioz Stars, 2020-07
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    93
    Promega lipofectamine 2000 reagent
    Subcellular localization of SV replicative complexes and different viral and cellular proteins. Panel A. Co-localization of viral transcription complexes and nucleocapsid aggregation sites in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 7 hpi, the medium was supplemented with dactinomycin (2.5 µg/ml) for one hour. Cells were then transfected with a mixture of bromouridine (10 mM) and <t>Lipofectamine</t> 2000 reagent for 30 min. After this time, the transfection medium was replaced by 10% FCS supplemented with dactinomycin for 30 min. Immunofluorescence analysis was carried out as described in Materials and Methods . Panel B. Co-localization analyses of capsid protein and translation factors in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 8 hpi, cells were processed for immunofluorescence.
    Lipofectamine 2000 Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 2000 reagent/product/Promega
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    lipofectamine 2000 reagent - by Bioz Stars, 2020-07
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    92
    Roche lipofectamine 2000 reagent
    Subcellular localization of SV replicative complexes and different viral and cellular proteins. Panel A. Co-localization of viral transcription complexes and nucleocapsid aggregation sites in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 7 hpi, the medium was supplemented with dactinomycin (2.5 µg/ml) for one hour. Cells were then transfected with a mixture of bromouridine (10 mM) and <t>Lipofectamine</t> 2000 reagent for 30 min. After this time, the transfection medium was replaced by 10% FCS supplemented with dactinomycin for 30 min. Immunofluorescence analysis was carried out as described in Materials and Methods . Panel B. Co-localization analyses of capsid protein and translation factors in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 8 hpi, cells were processed for immunofluorescence.
    Lipofectamine 2000 Reagent, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 2000 reagent/product/Roche
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    lipofectamine 2000 reagent - by Bioz Stars, 2020-07
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    92
    tiangen biotech co lipofectamine 2000 reagent
    Subcellular localization of SV replicative complexes and different viral and cellular proteins. Panel A. Co-localization of viral transcription complexes and nucleocapsid aggregation sites in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 7 hpi, the medium was supplemented with dactinomycin (2.5 µg/ml) for one hour. Cells were then transfected with a mixture of bromouridine (10 mM) and <t>Lipofectamine</t> 2000 reagent for 30 min. After this time, the transfection medium was replaced by 10% FCS supplemented with dactinomycin for 30 min. Immunofluorescence analysis was carried out as described in Materials and Methods . Panel B. Co-localization analyses of capsid protein and translation factors in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 8 hpi, cells were processed for immunofluorescence.
    Lipofectamine 2000 Reagent, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    lipofectamine 2000 reagent - by Bioz Stars, 2020-07
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    92
    GE Healthcare lipofectamine 2000 reagent
    Subcellular localization of SV replicative complexes and different viral and cellular proteins. Panel A. Co-localization of viral transcription complexes and nucleocapsid aggregation sites in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 7 hpi, the medium was supplemented with dactinomycin (2.5 µg/ml) for one hour. Cells were then transfected with a mixture of bromouridine (10 mM) and <t>Lipofectamine</t> 2000 reagent for 30 min. After this time, the transfection medium was replaced by 10% FCS supplemented with dactinomycin for 30 min. Immunofluorescence analysis was carried out as described in Materials and Methods . Panel B. Co-localization analyses of capsid protein and translation factors in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 8 hpi, cells were processed for immunofluorescence.
    Lipofectamine 2000 Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 2000 reagent/product/GE Healthcare
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    91
    Becton Dickinson lipofectamine 2000 reagent
    Subcellular localization of SV replicative complexes and different viral and cellular proteins. Panel A. Co-localization of viral transcription complexes and nucleocapsid aggregation sites in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 7 hpi, the medium was supplemented with dactinomycin (2.5 µg/ml) for one hour. Cells were then transfected with a mixture of bromouridine (10 mM) and <t>Lipofectamine</t> 2000 reagent for 30 min. After this time, the transfection medium was replaced by 10% FCS supplemented with dactinomycin for 30 min. Immunofluorescence analysis was carried out as described in Materials and Methods . Panel B. Co-localization analyses of capsid protein and translation factors in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 8 hpi, cells were processed for immunofluorescence.
    Lipofectamine 2000 Reagent, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    GenePharma Company lipofectamine 2000 reagent
    Subcellular localization of SV replicative complexes and different viral and cellular proteins. Panel A. Co-localization of viral transcription complexes and nucleocapsid aggregation sites in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 7 hpi, the medium was supplemented with dactinomycin (2.5 µg/ml) for one hour. Cells were then transfected with a mixture of bromouridine (10 mM) and <t>Lipofectamine</t> 2000 reagent for 30 min. After this time, the transfection medium was replaced by 10% FCS supplemented with dactinomycin for 30 min. Immunofluorescence analysis was carried out as described in Materials and Methods . Panel B. Co-localization analyses of capsid protein and translation factors in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 8 hpi, cells were processed for immunofluorescence.
    Lipofectamine 2000 Reagent, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher plasmids lipofectamine 2000 reagent
    Subcellular localization of SV replicative complexes and different viral and cellular proteins. Panel A. Co-localization of viral transcription complexes and nucleocapsid aggregation sites in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 7 hpi, the medium was supplemented with dactinomycin (2.5 µg/ml) for one hour. Cells were then transfected with a mixture of bromouridine (10 mM) and <t>Lipofectamine</t> 2000 reagent for 30 min. After this time, the transfection medium was replaced by 10% FCS supplemented with dactinomycin for 30 min. Immunofluorescence analysis was carried out as described in Materials and Methods . Panel B. Co-localization analyses of capsid protein and translation factors in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 8 hpi, cells were processed for immunofluorescence.
    Plasmids Lipofectamine 2000 Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher materials lipofectamine 2000 reagent
    Subcellular localization of SV replicative complexes and different viral and cellular proteins. Panel A. Co-localization of viral transcription complexes and nucleocapsid aggregation sites in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 7 hpi, the medium was supplemented with dactinomycin (2.5 µg/ml) for one hour. Cells were then transfected with a mixture of bromouridine (10 mM) and <t>Lipofectamine</t> 2000 reagent for 30 min. After this time, the transfection medium was replaced by 10% FCS supplemented with dactinomycin for 30 min. Immunofluorescence analysis was carried out as described in Materials and Methods . Panel B. Co-localization analyses of capsid protein and translation factors in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 8 hpi, cells were processed for immunofluorescence.
    Materials Lipofectamine 2000 Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher transfection reagent lipofectamine 2000 reagent
    Fluorescence intensities of NCI-H441 cells transfected with ASOgreen and 5′Fam-ASOgreen. Cells were transfected with the fluorescent 5′Fam-ASOgreen as well as the non-fluorescent ASOgreen as controls. <t>Lipofectamine</t> and CS were used as <t>transfection</t> reagents. Total RGB fluorescence intensity was determined 24 h after transfection (non-significant (ns), p ≤ 0.001 (***), p ≤ 0.0001 (****); n = 12).
    Transfection Reagent Lipofectamine 2000 Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effects of dominant negative form of monomeric GTP-binding proteins on the induction of the promoter activity of IL-6 by H. pylori infection. MKN28 cells were cotransfected with 0.5 μg of the p1168hu.IL6P-luc+ plasmid together with 0.3 μg of one of the four plasmids, pCMV- RhoN19 , pCMV- RacN17 , pCMV- RasN17 , or pCMV plus 10 ng of Renilla plasmid as an internal control using LipofectAMINE. The transfected cells were subsequently infected with H. pylori . MKN28 cells were also cotransfected with the p1168hu.IL6P-luc+ plasmid together with either 50 ng of pCMV or pCMV-RafS621A plus 10 ng of Renilla plasmid and subsequently infected with H. pylori . Untreated plates served as controls. Shown is the normalized luciferase activity expressed as fold increase of luciferase activity in H. pylori -infected cells relative to uninfected controls. Five independent transfections, each run in triplicate, were performed. Data are expressed as mean ± SE; ** p

    Journal: Molecular Biology of the Cell

    Article Title: Regulation of Interleukin-6 Promoter Activation in Gastric Epithelial Cells Infected with Helicobacter pylori

    doi: 10.1091/mbc.E05-05-0426

    Figure Lengend Snippet: Effects of dominant negative form of monomeric GTP-binding proteins on the induction of the promoter activity of IL-6 by H. pylori infection. MKN28 cells were cotransfected with 0.5 μg of the p1168hu.IL6P-luc+ plasmid together with 0.3 μg of one of the four plasmids, pCMV- RhoN19 , pCMV- RacN17 , pCMV- RasN17 , or pCMV plus 10 ng of Renilla plasmid as an internal control using LipofectAMINE. The transfected cells were subsequently infected with H. pylori . MKN28 cells were also cotransfected with the p1168hu.IL6P-luc+ plasmid together with either 50 ng of pCMV or pCMV-RafS621A plus 10 ng of Renilla plasmid and subsequently infected with H. pylori . Untreated plates served as controls. Shown is the normalized luciferase activity expressed as fold increase of luciferase activity in H. pylori -infected cells relative to uninfected controls. Five independent transfections, each run in triplicate, were performed. Data are expressed as mean ± SE; ** p

    Article Snippet: MKN28 cells were transfected with TranSilent shRNA Vectors for NF-κB p50 and NF-κB p65 (0.75 μg for each) or 1.5 μg of empty vectors (Panomics, Redwood City, CA) using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer's instructions.

    Techniques: Dominant Negative Mutation, Binding Assay, Activity Assay, Infection, Plasmid Preparation, Transfection, Luciferase

    LC3 puncta formation is increased in the HeLa cells at 24 h.p.i. HeLa cells were transfected with plasmid pEGFP-LC3 or the empty vector (pEGFP-C3) using Lipofectamine 2000 (Invitrogen Life Technologies). Twenty-four hours later, fluorescence microscopy demonstrated the successful transduction of cells with pEGFP-LC3 and pEGFP-C3. Then, the cells were seeded in 6-well plates. (B-D) Twenty-four hours later, the cells were infected with CVB3, and (A) mock-infected cells served as the control group. LC3, light chain 3; h.p.i., hours post-inoculation; CVB3, coxsackievirus B3.

    Journal: International Journal of Molecular Medicine

    Article Title: The PI3K/Akt/mTOR pathway is involved in CVB3-induced autophagy of HeLa cells

    doi: 10.3892/ijmm.2017.3008

    Figure Lengend Snippet: LC3 puncta formation is increased in the HeLa cells at 24 h.p.i. HeLa cells were transfected with plasmid pEGFP-LC3 or the empty vector (pEGFP-C3) using Lipofectamine 2000 (Invitrogen Life Technologies). Twenty-four hours later, fluorescence microscopy demonstrated the successful transduction of cells with pEGFP-LC3 and pEGFP-C3. Then, the cells were seeded in 6-well plates. (B-D) Twenty-four hours later, the cells were infected with CVB3, and (A) mock-infected cells served as the control group. LC3, light chain 3; h.p.i., hours post-inoculation; CVB3, coxsackievirus B3.

    Article Snippet: Four milligrams of expression plasmid combined with 10 µ l Lipofectamine 2000 reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) was added to the cells according to the manufacturer's instructions.

    Techniques: Transfection, Plasmid Preparation, Fluorescence, Microscopy, Transduction, Infection

    siIQGAP1 decreases the stability of Nrf2 and attenuates HO-1 expression and ARE-luciferase activity. (A) HeLa cells were transfected with siIQGAP1 for 72 h using Lipofectamin 2000 reagent to silence or knockdown the endogenous IQGAP1 gene expression.

    Journal: Antioxidants & Redox Signaling

    Article Title: Identification and Functional Studies of a New Nrf2 Partner IQGAP1: A Critical Role in the Stability and Transactivation of Nrf2

    doi: 10.1089/ars.2012.4586

    Figure Lengend Snippet: siIQGAP1 decreases the stability of Nrf2 and attenuates HO-1 expression and ARE-luciferase activity. (A) HeLa cells were transfected with siIQGAP1 for 72 h using Lipofectamin 2000 reagent to silence or knockdown the endogenous IQGAP1 gene expression.

    Article Snippet: Minimum essential medium (MEM), fetal bovine serum (FBS), penicillin/streptomycin antibiotics mixture, lipofectamin 2000 reagent, and Dynabead G were obtained from Invitrogen.

    Techniques: Expressing, Luciferase, Activity Assay, Transfection

    Subcellular localization of SV replicative complexes and different viral and cellular proteins. Panel A. Co-localization of viral transcription complexes and nucleocapsid aggregation sites in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 7 hpi, the medium was supplemented with dactinomycin (2.5 µg/ml) for one hour. Cells were then transfected with a mixture of bromouridine (10 mM) and Lipofectamine 2000 reagent for 30 min. After this time, the transfection medium was replaced by 10% FCS supplemented with dactinomycin for 30 min. Immunofluorescence analysis was carried out as described in Materials and Methods . Panel B. Co-localization analyses of capsid protein and translation factors in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 8 hpi, cells were processed for immunofluorescence.

    Journal: PLoS ONE

    Article Title: Dual Mechanism for the Translation of Subgenomic mRNA from Sindbis Virus in Infected and Uninfected Cells

    doi: 10.1371/journal.pone.0004772

    Figure Lengend Snippet: Subcellular localization of SV replicative complexes and different viral and cellular proteins. Panel A. Co-localization of viral transcription complexes and nucleocapsid aggregation sites in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 7 hpi, the medium was supplemented with dactinomycin (2.5 µg/ml) for one hour. Cells were then transfected with a mixture of bromouridine (10 mM) and Lipofectamine 2000 reagent for 30 min. After this time, the transfection medium was replaced by 10% FCS supplemented with dactinomycin for 30 min. Immunofluorescence analysis was carried out as described in Materials and Methods . Panel B. Co-localization analyses of capsid protein and translation factors in SV-infected cells. BHK cells were infected with SV (100 pfu/cell) and, at 8 hpi, cells were processed for immunofluorescence.

    Article Snippet: Plasmids were transfected using Lipofectamine 2000 reagent (Promega) as recommended by the supplier.

    Techniques: Infection, Transfection, Immunofluorescence

    Fluorescence intensities of NCI-H441 cells transfected with ASOgreen and 5′Fam-ASOgreen. Cells were transfected with the fluorescent 5′Fam-ASOgreen as well as the non-fluorescent ASOgreen as controls. Lipofectamine and CS were used as transfection reagents. Total RGB fluorescence intensity was determined 24 h after transfection (non-significant (ns), p ≤ 0.001 (***), p ≤ 0.0001 (****); n = 12).

    Journal: Biomolecules

    Article Title: Chitosan Nanocomplexes for the Delivery of ENaC Antisense Oligonucleotides to Airway Epithelial Cells

    doi: 10.3390/biom10040553

    Figure Lengend Snippet: Fluorescence intensities of NCI-H441 cells transfected with ASOgreen and 5′Fam-ASOgreen. Cells were transfected with the fluorescent 5′Fam-ASOgreen as well as the non-fluorescent ASOgreen as controls. Lipofectamine and CS were used as transfection reagents. Total RGB fluorescence intensity was determined 24 h after transfection (non-significant (ns), p ≤ 0.001 (***), p ≤ 0.0001 (****); n = 12).

    Article Snippet: As a control, cells were transfected using the commercially available transfection reagent Lipofectamine® 2000 Reagent (Lipofectamine; Invitrogen, Karlsruhe, Germany).

    Techniques: Fluorescence, Transfection

    Representative confocal laser scanning microscope (CLSM) images of NCI-H441 cells transfected with 5′Fam-ASOgreen. Cells were transfected using ( a – c ) Lipofectamine and ( d – f ) CS. Images were taken 24 h after transfection. ( a , d ) DAPI; ( b , e ) 5′Fam-ASOgreen; ( c , f ) Merge (scale bar = 20 µm).

    Journal: Biomolecules

    Article Title: Chitosan Nanocomplexes for the Delivery of ENaC Antisense Oligonucleotides to Airway Epithelial Cells

    doi: 10.3390/biom10040553

    Figure Lengend Snippet: Representative confocal laser scanning microscope (CLSM) images of NCI-H441 cells transfected with 5′Fam-ASOgreen. Cells were transfected using ( a – c ) Lipofectamine and ( d – f ) CS. Images were taken 24 h after transfection. ( a , d ) DAPI; ( b , e ) 5′Fam-ASOgreen; ( c , f ) Merge (scale bar = 20 µm).

    Article Snippet: As a control, cells were transfected using the commercially available transfection reagent Lipofectamine® 2000 Reagent (Lipofectamine; Invitrogen, Karlsruhe, Germany).

    Techniques: Laser-Scanning Microscopy, Confocal Laser Scanning Microscopy, Transfection

    Transepithelial Ussing chamber measurements of NCI-H441 cells. ( a ) Representative time course of non-transfected cells, showing a slight decrease in short-circuit current (I sc ) after application of Na + -free ringer solution. ( b ) Representative time course of cells transfected with ASOgreen using CS (0.45 μg/cm² ASOgreen), showing a strongly decreased I sc after withdrawal of Na + . Please note the different scales for I sc . ( c ) Statistical evaluation of amiloride-sensitive short-circuit current (ΔI sc ) in NCI-H441 cells after ASO transfection with Lipofectamine and CS. Cells were transfected with 0.45 μg/cm² ASO, respectively; control cells were not transfected. Measurements were conducted 24 h after transfection. The amiloride-sensitive current decreased after transfection with ASOgreen. Cells transfected with ASOgreen_sense only showed a slight decrease in the amiloride-sensitive current ( p ≤ 0.01 (**); control, n = 8; ASOgreen, n = 7; ASOgreen_sense, n = 5).

    Journal: Biomolecules

    Article Title: Chitosan Nanocomplexes for the Delivery of ENaC Antisense Oligonucleotides to Airway Epithelial Cells

    doi: 10.3390/biom10040553

    Figure Lengend Snippet: Transepithelial Ussing chamber measurements of NCI-H441 cells. ( a ) Representative time course of non-transfected cells, showing a slight decrease in short-circuit current (I sc ) after application of Na + -free ringer solution. ( b ) Representative time course of cells transfected with ASOgreen using CS (0.45 μg/cm² ASOgreen), showing a strongly decreased I sc after withdrawal of Na + . Please note the different scales for I sc . ( c ) Statistical evaluation of amiloride-sensitive short-circuit current (ΔI sc ) in NCI-H441 cells after ASO transfection with Lipofectamine and CS. Cells were transfected with 0.45 μg/cm² ASO, respectively; control cells were not transfected. Measurements were conducted 24 h after transfection. The amiloride-sensitive current decreased after transfection with ASOgreen. Cells transfected with ASOgreen_sense only showed a slight decrease in the amiloride-sensitive current ( p ≤ 0.01 (**); control, n = 8; ASOgreen, n = 7; ASOgreen_sense, n = 5).

    Article Snippet: As a control, cells were transfected using the commercially available transfection reagent Lipofectamine® 2000 Reagent (Lipofectamine; Invitrogen, Karlsruhe, Germany).

    Techniques: Transfection, Allele-specific Oligonucleotide