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  • 99
    Thermo Fisher block it fluorescent oligo
    Block It Fluorescent Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/block it fluorescent oligo/product/Thermo Fisher
    Average 99 stars, based on 1335 article reviews
    Price from $9.99 to $1999.99
    block it fluorescent oligo - by Bioz Stars, 2021-01
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    99
    Thermo Fisher cationic lipid
    The in vitro transcription and expression of TsSP1.2 in transfected BHK-21 cells. A TsSP1.2 mRNA transcription in BHK-21 cells was detected by RT-PCR. l. M: DL2000 marker; 1: pcDNA3.1-TsSP1.2 transfected cells; 2: empty pcDNA3.1 transfected cells; 3: non-transfected normal cells; 4: <t>Lipofectamine</t> 2000 control. B TsSP1.2 expression in BHK-21 cells transfected with pcDNA3.1-TsSP1.2 was observed by IFT with anti-rTsSP1.2 serum. C The pcDNA3.1-transfected BHK-21 cells were used as negative control. The scale bar is 50 μm.
    Cationic Lipid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cationic lipid/product/Thermo Fisher
    Average 99 stars, based on 279 article reviews
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    cationic lipid - by Bioz Stars, 2021-01
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    99
    Thermo Fisher lipofectamine rnaimax lipid reagent
    The in vitro transcription and expression of TsSP1.2 in transfected BHK-21 cells. A TsSP1.2 mRNA transcription in BHK-21 cells was detected by RT-PCR. l. M: DL2000 marker; 1: pcDNA3.1-TsSP1.2 transfected cells; 2: empty pcDNA3.1 transfected cells; 3: non-transfected normal cells; 4: <t>Lipofectamine</t> 2000 control. B TsSP1.2 expression in BHK-21 cells transfected with pcDNA3.1-TsSP1.2 was observed by IFT with anti-rTsSP1.2 serum. C The pcDNA3.1-transfected BHK-21 cells were used as negative control. The scale bar is 50 μm.
    Lipofectamine Rnaimax Lipid Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine rnaimax lipid reagent/product/Thermo Fisher
    Average 99 stars, based on 116 article reviews
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    99
    Thermo Fisher siport lipid transfection agent
    The in vitro transcription and expression of TsSP1.2 in transfected BHK-21 cells. A TsSP1.2 mRNA transcription in BHK-21 cells was detected by RT-PCR. l. M: DL2000 marker; 1: pcDNA3.1-TsSP1.2 transfected cells; 2: empty pcDNA3.1 transfected cells; 3: non-transfected normal cells; 4: <t>Lipofectamine</t> 2000 control. B TsSP1.2 expression in BHK-21 cells transfected with pcDNA3.1-TsSP1.2 was observed by IFT with anti-rTsSP1.2 serum. C The pcDNA3.1-transfected BHK-21 cells were used as negative control. The scale bar is 50 μm.
    Siport Lipid Transfection Agent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad transfectin transfection reagent
    The in vitro transcription and expression of TsSP1.2 in transfected BHK-21 cells. A TsSP1.2 mRNA transcription in BHK-21 cells was detected by RT-PCR. l. M: DL2000 marker; 1: pcDNA3.1-TsSP1.2 transfected cells; 2: empty pcDNA3.1 transfected cells; 3: non-transfected normal cells; 4: <t>Lipofectamine</t> 2000 control. B TsSP1.2 expression in BHK-21 cells transfected with pcDNA3.1-TsSP1.2 was observed by IFT with anti-rTsSP1.2 serum. C The pcDNA3.1-transfected BHK-21 cells were used as negative control. The scale bar is 50 μm.
    Transfectin Transfection Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 92 article reviews
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    85
    SignaGen genjet lipid transfection reagents
    The in vitro transcription and expression of TsSP1.2 in transfected BHK-21 cells. A TsSP1.2 mRNA transcription in BHK-21 cells was detected by RT-PCR. l. M: DL2000 marker; 1: pcDNA3.1-TsSP1.2 transfected cells; 2: empty pcDNA3.1 transfected cells; 3: non-transfected normal cells; 4: <t>Lipofectamine</t> 2000 control. B TsSP1.2 expression in BHK-21 cells transfected with pcDNA3.1-TsSP1.2 was observed by IFT with anti-rTsSP1.2 serum. C The pcDNA3.1-transfected BHK-21 cells were used as negative control. The scale bar is 50 μm.
    Genjet Lipid Transfection Reagents, supplied by SignaGen, used in various techniques. Bioz Stars score: 85/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    genjet lipid transfection reagents - by Bioz Stars, 2021-01
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    N/A
    0 5 ml 1 mg ml lipid transfection reagent RNAi specific lipid for siRNA delivery
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    N/A
    0 5 ml 1 5 mg ml lipid transfection reagent for a variety of applications including plasmid DNA siRNA and shRNA delivery
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    The in vitro transcription and expression of TsSP1.2 in transfected BHK-21 cells. A TsSP1.2 mRNA transcription in BHK-21 cells was detected by RT-PCR. l. M: DL2000 marker; 1: pcDNA3.1-TsSP1.2 transfected cells; 2: empty pcDNA3.1 transfected cells; 3: non-transfected normal cells; 4: Lipofectamine 2000 control. B TsSP1.2 expression in BHK-21 cells transfected with pcDNA3.1-TsSP1.2 was observed by IFT with anti-rTsSP1.2 serum. C The pcDNA3.1-transfected BHK-21 cells were used as negative control. The scale bar is 50 μm.

    Journal: Veterinary Research

    Article Title: Protective immunity against Trichinella spiralis in mice elicited by oral vaccination with attenuated Salmonella-delivered TsSP1.2 DNA

    doi: 10.1186/s13567-018-0582-2

    Figure Lengend Snippet: The in vitro transcription and expression of TsSP1.2 in transfected BHK-21 cells. A TsSP1.2 mRNA transcription in BHK-21 cells was detected by RT-PCR. l. M: DL2000 marker; 1: pcDNA3.1-TsSP1.2 transfected cells; 2: empty pcDNA3.1 transfected cells; 3: non-transfected normal cells; 4: Lipofectamine 2000 control. B TsSP1.2 expression in BHK-21 cells transfected with pcDNA3.1-TsSP1.2 was observed by IFT with anti-rTsSP1.2 serum. C The pcDNA3.1-transfected BHK-21 cells were used as negative control. The scale bar is 50 μm.

    Article Snippet: When the BHK 21 cells were grown to 90% confluence, the cells were collected by trypsinization and transfected with pcDNA3.1-TsSP1.2 with a cationic lipid Lipofectamine 2000 (Invitrogen, USA) at the ratio of 0.8 μg DNA: 2 μL lipid per well in serum-free DMEM media at 37 °C for 48 h. Total RNA was extracted from the BHK-21 cells 24 h after transfection, and the transcription levels of TsSP1.2 mRNA in transfected cells were assayed by RT-PCR with TsSP1.2-specific primers as listed above [ ].

    Techniques: In Vitro, Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Marker, Negative Control

    Copy number analysis. a Copy number profiles of each tumor. Differences in color intensity depend on copy number amplitude and tumor purity. b Broad copy number changes enriched in the metastases ( n = 32) compared with TCGA tumors ( n = 80). Two-tailed Fisher’s exact tests with adjustment for multiple testing using the Benjamini–Hochberg method. c Two primary tumors compared with matched metastases. d Focal deletions of CDKN2A in two samples. e RNA-seq from the UM9 metastasis and corresponding PDX showing the region with focal CDKN2A deletion. f Genes in recurrent arm-level copy number aberrations ranked by associations between gene expression and copy number that were consistent in this cohort and TCGA tumors, and further ranked by protein–protein interaction network degree from the Human Protein Reference Database (HPRD), and additionally by presence of any associations with worse survival. The top three candidates are shown in each region. Connecting lines represent protein interactions of the highest ranked gene per region. Blue represents regions of loss and red regions of gain. Summarized representations of copy number profiles per region show the relative numbers of gain and loss events, with the inner circle representing TCGA samples and the outer our cohort. For the metastatic cohort, n = 28 samples with matching DNA and RNA data were included. g Pathways enriched among the combined set of the top 10 genes per region of gain or loss. h Functional interrogation by siRNA of main candidate genes whose expression is elevated due to copy number alteration. Cells were counted or viability was measured at 72 h, 96 h and 96 h for the cell lines UM22, MP-41, and 92-1, respectively, after transfection of the siRNA pools. n = 3 samples were transfected independently, for each cell line. Data are presented as mean values ± standard error of the mean (SEM). Two-way ANOVA was used to estimate differences, taking into account both cell line and target gene as variables. q values were calculated using Benjamini–Hochberg correction, taking into account all genes in h as well as those in Supplementary Fig. 3c , which shows other candidates of interest investigated. Dotted lines indicate q = 0.05.

    Journal: Nature Communications

    Article Title: Molecular profiling of driver events in metastatic uveal melanoma

    doi: 10.1038/s41467-020-15606-0

    Figure Lengend Snippet: Copy number analysis. a Copy number profiles of each tumor. Differences in color intensity depend on copy number amplitude and tumor purity. b Broad copy number changes enriched in the metastases ( n = 32) compared with TCGA tumors ( n = 80). Two-tailed Fisher’s exact tests with adjustment for multiple testing using the Benjamini–Hochberg method. c Two primary tumors compared with matched metastases. d Focal deletions of CDKN2A in two samples. e RNA-seq from the UM9 metastasis and corresponding PDX showing the region with focal CDKN2A deletion. f Genes in recurrent arm-level copy number aberrations ranked by associations between gene expression and copy number that were consistent in this cohort and TCGA tumors, and further ranked by protein–protein interaction network degree from the Human Protein Reference Database (HPRD), and additionally by presence of any associations with worse survival. The top three candidates are shown in each region. Connecting lines represent protein interactions of the highest ranked gene per region. Blue represents regions of loss and red regions of gain. Summarized representations of copy number profiles per region show the relative numbers of gain and loss events, with the inner circle representing TCGA samples and the outer our cohort. For the metastatic cohort, n = 28 samples with matching DNA and RNA data were included. g Pathways enriched among the combined set of the top 10 genes per region of gain or loss. h Functional interrogation by siRNA of main candidate genes whose expression is elevated due to copy number alteration. Cells were counted or viability was measured at 72 h, 96 h and 96 h for the cell lines UM22, MP-41, and 92-1, respectively, after transfection of the siRNA pools. n = 3 samples were transfected independently, for each cell line. Data are presented as mean values ± standard error of the mean (SEM). Two-way ANOVA was used to estimate differences, taking into account both cell line and target gene as variables. q values were calculated using Benjamini–Hochberg correction, taking into account all genes in h as well as those in Supplementary Fig. 3c , which shows other candidates of interest investigated. Dotted lines indicate q = 0.05.

    Article Snippet: The siRNA duplexes were purchased from Dharmacon (Thermo Fisher Scientific, Waltham, MA, USA) and the lipid based transfection was performed with Lipofectamine-RNAiMAX (Thermo Fisher Scientific, Waltham, MA, USA) using 1 pmol of siRNA per well of a 96-well plate as per the guidelines provided by manufacturer.

    Techniques: Two Tailed Test, RNA Sequencing Assay, Expressing, Functional Assay, Transfection

    MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Journal: The Prostate

    Article Title: Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer. Overexpression of MCM10 promotes cell proliferation and predicts poor prognosis in prostate cancer

    doi: 10.1002/pros.23703

    Figure Lengend Snippet: MCM10 knockdown inhibits its expression levels in DU145 and PC‐3 cells. A‐C, Real‐time reverse transcription PCR analysis showed expression of MCM10 mRNA was reduced after transfection with the indicated shRNAs in DU145 (A) PC‐3 (B) and LNCaP (C) cells. D‐F, Western blot analysis showed protein expression of MCM10 was reduced after transfection with the indicated shRNAs in DU145 (D), PC‐3 (E), and LNCaP (F) cells. Data are represented as the mean ± SD (* P

    Article Snippet: Opti‐modified Eagle's medium (Opti‐MEM) which was ideal for use during cationic lipid transfections especially Lipofectamine™ transfection reagents was purchased from Thermo Fisher Scientific, Inc. (Catalog number: 31985062).

    Techniques: Expressing, Polymerase Chain Reaction, Transfection, Western Blot