lipid based transfection reagent Search Results


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  • 99
    Thermo Fisher lipid based transfection reagent
    Copy number analysis. a Copy number profiles of each tumor. Differences in color intensity depend on copy number amplitude and tumor purity. b Broad copy number changes enriched in the metastases ( n = 32) compared with TCGA tumors ( n = 80). Two-tailed Fisher’s exact tests with adjustment for multiple testing using the Benjamini–Hochberg method. c Two primary tumors compared with matched metastases. d Focal deletions of CDKN2A in two samples. e RNA-seq from the UM9 metastasis and corresponding PDX showing the region with focal CDKN2A deletion. f Genes in recurrent arm-level copy number aberrations ranked by associations between gene expression and copy number that were consistent in this cohort and TCGA tumors, and further ranked by protein–protein interaction network degree from the Human Protein Reference Database (HPRD), and additionally by presence of any associations with worse survival. The top three candidates are shown in each region. Connecting lines represent protein interactions of the highest ranked gene per region. Blue represents regions of loss and red regions of gain. Summarized representations of copy number profiles per region show the relative numbers of gain and loss events, with the inner circle representing TCGA samples and the outer our cohort. For the metastatic cohort, n = 28 samples with matching DNA and RNA data were included. g Pathways enriched among the combined set of the top 10 genes per region of gain or loss. h Functional interrogation by siRNA of main candidate genes whose expression is elevated due to copy number alteration. Cells were counted or viability was measured at 72 h, 96 h and 96 h for the cell lines UM22, MP-41, and 92-1, respectively, after <t>transfection</t> of the siRNA pools. n = 3 samples were transfected independently, for each cell line. Data are presented as mean values ± standard error of the mean (SEM). Two-way ANOVA was used to estimate differences, taking into account both cell line and target gene as variables. q values were calculated using Benjamini–Hochberg correction, taking into account all genes in h as well as those in Supplementary Fig. 3c , which shows other candidates of interest investigated. Dotted lines indicate q = 0.05.
    Lipid Based Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection reagent/product/Thermo Fisher
    Average 99 stars, based on 149 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection reagent - by Bioz Stars, 2020-11
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    88
    Mirus Bio lipid based transfection reagent
    Immunophenotype of primary DCs derived from (A) BALB/c and (B) C57BL6 mice, does not change after nonviral genetic <t>transfection</t> . The 2dDC were transfected with pVR1012-Ag2/PRA plasmid DNA. After 24 h of transfection, the cells were stained with fluorochrome-conjugated antibodies specific to MHC class II, CD11c, CD40, CD80, CD86 cell-surface antigens. The cell debris was gated-out and histogram charts were plotted. The filled histogram charts are of cells stained with isotype-control antibody. The mean fluorescent intensity (MFI) values were determined for the cells under the bar (⊓) region. The values within the charts indicate the MFI values for the nontransfected (NT, black line) and transfected (T, green line) cells stained with antibodies specific to cell-surface antigens. The results are from one representative experiment conducted thrice.
    Lipid Based Transfection Reagent, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 88/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection reagent/product/Mirus Bio
    Average 88 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection reagent - by Bioz Stars, 2020-11
    88/100 stars
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    99
    InvivoGen cationic lipid based transfection reagent
    Gbp4 and Gbp4 KS/AA localize to the speck in the presence of Asc. HEK293T cells were transfected with zebrafish Gbp4-GFP, Gbp4 KS/AA -GFP or Gbp4 ▵CARD -GFP in the presence or absence of zebrafish Asc-Myc, fixed at 48 h <t>post-transfection</t> and labelled in red with specific antibodies to the Myc epitope. ( a ) Representative frontal (xy) and lateral (xz and yz) views of maximum-intensity projection images of HEK293T cells stained with anti-Myc antibodies (Asc, Red). Gbp4, Gbp4 KS/AA and Gbp4 ▵CARD are visualized in green thanks to GFP, while nuclei are labelled with DAPI (blue). ( b ) Quantitation of the percentage of Gbp4 specks in relation to the total number of Gbp4 transfected cells. ( c ) Cells were lysed and anti-GFP and anti-c-Myc antibodies were used to validate the transfection assays by detecting Gbp4 and Asc, respectively, by western blot. The mass weights for all the proteins are indicated. Scale bars, 10 μm. The sample size for each treatment is Gbp4: 1300 cells; Gbp4+Asc: 1270 cells; Gbp4 KS/AA : 369 cells; Gbp4 KS/AA +Asc: 768 cells; Gbp4 ▵CARD : 773 cells; Gbp4 ▵CARD +Asc: 961cells. NS, not significant; *** P
    Cationic Lipid Based Transfection Reagent, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cationic lipid based transfection reagent/product/InvivoGen
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    cationic lipid based transfection reagent - by Bioz Stars, 2020-11
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    99
    Qiagen lipid based hiperfect transfection reagent
    Gbp4 and Gbp4 KS/AA localize to the speck in the presence of Asc. HEK293T cells were transfected with zebrafish Gbp4-GFP, Gbp4 KS/AA -GFP or Gbp4 ▵CARD -GFP in the presence or absence of zebrafish Asc-Myc, fixed at 48 h <t>post-transfection</t> and labelled in red with specific antibodies to the Myc epitope. ( a ) Representative frontal (xy) and lateral (xz and yz) views of maximum-intensity projection images of HEK293T cells stained with anti-Myc antibodies (Asc, Red). Gbp4, Gbp4 KS/AA and Gbp4 ▵CARD are visualized in green thanks to GFP, while nuclei are labelled with DAPI (blue). ( b ) Quantitation of the percentage of Gbp4 specks in relation to the total number of Gbp4 transfected cells. ( c ) Cells were lysed and anti-GFP and anti-c-Myc antibodies were used to validate the transfection assays by detecting Gbp4 and Asc, respectively, by western blot. The mass weights for all the proteins are indicated. Scale bars, 10 μm. The sample size for each treatment is Gbp4: 1300 cells; Gbp4+Asc: 1270 cells; Gbp4 KS/AA : 369 cells; Gbp4 KS/AA +Asc: 768 cells; Gbp4 ▵CARD : 773 cells; Gbp4 ▵CARD +Asc: 961cells. NS, not significant; *** P
    Lipid Based Hiperfect Transfection Reagent, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based hiperfect transfection reagent/product/Qiagen
    Average 99 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    lipid based hiperfect transfection reagent - by Bioz Stars, 2020-11
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    99
    Qiagen lipid based reagent effectene
    Gbp4 and Gbp4 KS/AA localize to the speck in the presence of Asc. HEK293T cells were transfected with zebrafish Gbp4-GFP, Gbp4 KS/AA -GFP or Gbp4 ▵CARD -GFP in the presence or absence of zebrafish Asc-Myc, fixed at 48 h <t>post-transfection</t> and labelled in red with specific antibodies to the Myc epitope. ( a ) Representative frontal (xy) and lateral (xz and yz) views of maximum-intensity projection images of HEK293T cells stained with anti-Myc antibodies (Asc, Red). Gbp4, Gbp4 KS/AA and Gbp4 ▵CARD are visualized in green thanks to GFP, while nuclei are labelled with DAPI (blue). ( b ) Quantitation of the percentage of Gbp4 specks in relation to the total number of Gbp4 transfected cells. ( c ) Cells were lysed and anti-GFP and anti-c-Myc antibodies were used to validate the transfection assays by detecting Gbp4 and Asc, respectively, by western blot. The mass weights for all the proteins are indicated. Scale bars, 10 μm. The sample size for each treatment is Gbp4: 1300 cells; Gbp4+Asc: 1270 cells; Gbp4 KS/AA : 369 cells; Gbp4 KS/AA +Asc: 768 cells; Gbp4 ▵CARD : 773 cells; Gbp4 ▵CARD +Asc: 961cells. NS, not significant; *** P
    Lipid Based Reagent Effectene, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based reagent effectene/product/Qiagen
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    lipid based reagent effectene - by Bioz Stars, 2020-11
    99/100 stars
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    88
    Horizon Discovery lipid based transfection reagents dharmafect1
    Gbp4 and Gbp4 KS/AA localize to the speck in the presence of Asc. HEK293T cells were transfected with zebrafish Gbp4-GFP, Gbp4 KS/AA -GFP or Gbp4 ▵CARD -GFP in the presence or absence of zebrafish Asc-Myc, fixed at 48 h <t>post-transfection</t> and labelled in red with specific antibodies to the Myc epitope. ( a ) Representative frontal (xy) and lateral (xz and yz) views of maximum-intensity projection images of HEK293T cells stained with anti-Myc antibodies (Asc, Red). Gbp4, Gbp4 KS/AA and Gbp4 ▵CARD are visualized in green thanks to GFP, while nuclei are labelled with DAPI (blue). ( b ) Quantitation of the percentage of Gbp4 specks in relation to the total number of Gbp4 transfected cells. ( c ) Cells were lysed and anti-GFP and anti-c-Myc antibodies were used to validate the transfection assays by detecting Gbp4 and Asc, respectively, by western blot. The mass weights for all the proteins are indicated. Scale bars, 10 μm. The sample size for each treatment is Gbp4: 1300 cells; Gbp4+Asc: 1270 cells; Gbp4 KS/AA : 369 cells; Gbp4 KS/AA +Asc: 768 cells; Gbp4 ▵CARD : 773 cells; Gbp4 ▵CARD +Asc: 961cells. NS, not significant; *** P
    Lipid Based Transfection Reagents Dharmafect1, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection reagents dharmafect1/product/Horizon Discovery
    Average 88 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection reagents dharmafect1 - by Bioz Stars, 2020-11
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    99
    Thermo Fisher lipid based transfection reagent lipofectamine 3000
    Gbp4 and Gbp4 KS/AA localize to the speck in the presence of Asc. HEK293T cells were transfected with zebrafish Gbp4-GFP, Gbp4 KS/AA -GFP or Gbp4 ▵CARD -GFP in the presence or absence of zebrafish Asc-Myc, fixed at 48 h <t>post-transfection</t> and labelled in red with specific antibodies to the Myc epitope. ( a ) Representative frontal (xy) and lateral (xz and yz) views of maximum-intensity projection images of HEK293T cells stained with anti-Myc antibodies (Asc, Red). Gbp4, Gbp4 KS/AA and Gbp4 ▵CARD are visualized in green thanks to GFP, while nuclei are labelled with DAPI (blue). ( b ) Quantitation of the percentage of Gbp4 specks in relation to the total number of Gbp4 transfected cells. ( c ) Cells were lysed and anti-GFP and anti-c-Myc antibodies were used to validate the transfection assays by detecting Gbp4 and Asc, respectively, by western blot. The mass weights for all the proteins are indicated. Scale bars, 10 μm. The sample size for each treatment is Gbp4: 1300 cells; Gbp4+Asc: 1270 cells; Gbp4 KS/AA : 369 cells; Gbp4 KS/AA +Asc: 768 cells; Gbp4 ▵CARD : 773 cells; Gbp4 ▵CARD +Asc: 961cells. NS, not significant; *** P
    Lipid Based Transfection Reagent Lipofectamine 3000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection reagent lipofectamine 3000/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection reagent lipofectamine 3000 - by Bioz Stars, 2020-11
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    99
    Promega lipid based transfection reagent fugene 6 hd
    Gbp4 and Gbp4 KS/AA localize to the speck in the presence of Asc. HEK293T cells were transfected with zebrafish Gbp4-GFP, Gbp4 KS/AA -GFP or Gbp4 ▵CARD -GFP in the presence or absence of zebrafish Asc-Myc, fixed at 48 h <t>post-transfection</t> and labelled in red with specific antibodies to the Myc epitope. ( a ) Representative frontal (xy) and lateral (xz and yz) views of maximum-intensity projection images of HEK293T cells stained with anti-Myc antibodies (Asc, Red). Gbp4, Gbp4 KS/AA and Gbp4 ▵CARD are visualized in green thanks to GFP, while nuclei are labelled with DAPI (blue). ( b ) Quantitation of the percentage of Gbp4 specks in relation to the total number of Gbp4 transfected cells. ( c ) Cells were lysed and anti-GFP and anti-c-Myc antibodies were used to validate the transfection assays by detecting Gbp4 and Asc, respectively, by western blot. The mass weights for all the proteins are indicated. Scale bars, 10 μm. The sample size for each treatment is Gbp4: 1300 cells; Gbp4+Asc: 1270 cells; Gbp4 KS/AA : 369 cells; Gbp4 KS/AA +Asc: 768 cells; Gbp4 ▵CARD : 773 cells; Gbp4 ▵CARD +Asc: 961cells. NS, not significant; *** P
    Lipid Based Transfection Reagent Fugene 6 Hd, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection reagent fugene 6 hd/product/Promega
    Average 99 stars, based on 4 article reviews
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    99
    Bio-Rad lipid based transfection reagent
    DNA <t>transfection</t> of neuronal cultures and measured by reporter gene assay Culture cells were transfected with pGL3, BACE1- (551 bp) and hAPP (1.2 kb of upstream regulatory region) reporter plasmids at day 13. Signals were obtained by Luciferase based assay (normalization was done by raw protein OD-595). Results show that expression of both human BACE1 and human APP transfected promoter constructs are easily detectable.
    Lipid Based Transfection Reagent, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection reagent/product/Bio-Rad
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection reagent - by Bioz Stars, 2020-11
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    Image Search Results


    Copy number analysis. a Copy number profiles of each tumor. Differences in color intensity depend on copy number amplitude and tumor purity. b Broad copy number changes enriched in the metastases ( n = 32) compared with TCGA tumors ( n = 80). Two-tailed Fisher’s exact tests with adjustment for multiple testing using the Benjamini–Hochberg method. c Two primary tumors compared with matched metastases. d Focal deletions of CDKN2A in two samples. e RNA-seq from the UM9 metastasis and corresponding PDX showing the region with focal CDKN2A deletion. f Genes in recurrent arm-level copy number aberrations ranked by associations between gene expression and copy number that were consistent in this cohort and TCGA tumors, and further ranked by protein–protein interaction network degree from the Human Protein Reference Database (HPRD), and additionally by presence of any associations with worse survival. The top three candidates are shown in each region. Connecting lines represent protein interactions of the highest ranked gene per region. Blue represents regions of loss and red regions of gain. Summarized representations of copy number profiles per region show the relative numbers of gain and loss events, with the inner circle representing TCGA samples and the outer our cohort. For the metastatic cohort, n = 28 samples with matching DNA and RNA data were included. g Pathways enriched among the combined set of the top 10 genes per region of gain or loss. h Functional interrogation by siRNA of main candidate genes whose expression is elevated due to copy number alteration. Cells were counted or viability was measured at 72 h, 96 h and 96 h for the cell lines UM22, MP-41, and 92-1, respectively, after transfection of the siRNA pools. n = 3 samples were transfected independently, for each cell line. Data are presented as mean values ± standard error of the mean (SEM). Two-way ANOVA was used to estimate differences, taking into account both cell line and target gene as variables. q values were calculated using Benjamini–Hochberg correction, taking into account all genes in h as well as those in Supplementary Fig. 3c , which shows other candidates of interest investigated. Dotted lines indicate q = 0.05.

    Journal: Nature Communications

    Article Title: Molecular profiling of driver events in metastatic uveal melanoma

    doi: 10.1038/s41467-020-15606-0

    Figure Lengend Snippet: Copy number analysis. a Copy number profiles of each tumor. Differences in color intensity depend on copy number amplitude and tumor purity. b Broad copy number changes enriched in the metastases ( n = 32) compared with TCGA tumors ( n = 80). Two-tailed Fisher’s exact tests with adjustment for multiple testing using the Benjamini–Hochberg method. c Two primary tumors compared with matched metastases. d Focal deletions of CDKN2A in two samples. e RNA-seq from the UM9 metastasis and corresponding PDX showing the region with focal CDKN2A deletion. f Genes in recurrent arm-level copy number aberrations ranked by associations between gene expression and copy number that were consistent in this cohort and TCGA tumors, and further ranked by protein–protein interaction network degree from the Human Protein Reference Database (HPRD), and additionally by presence of any associations with worse survival. The top three candidates are shown in each region. Connecting lines represent protein interactions of the highest ranked gene per region. Blue represents regions of loss and red regions of gain. Summarized representations of copy number profiles per region show the relative numbers of gain and loss events, with the inner circle representing TCGA samples and the outer our cohort. For the metastatic cohort, n = 28 samples with matching DNA and RNA data were included. g Pathways enriched among the combined set of the top 10 genes per region of gain or loss. h Functional interrogation by siRNA of main candidate genes whose expression is elevated due to copy number alteration. Cells were counted or viability was measured at 72 h, 96 h and 96 h for the cell lines UM22, MP-41, and 92-1, respectively, after transfection of the siRNA pools. n = 3 samples were transfected independently, for each cell line. Data are presented as mean values ± standard error of the mean (SEM). Two-way ANOVA was used to estimate differences, taking into account both cell line and target gene as variables. q values were calculated using Benjamini–Hochberg correction, taking into account all genes in h as well as those in Supplementary Fig. 3c , which shows other candidates of interest investigated. Dotted lines indicate q = 0.05.

    Article Snippet: The siRNA duplexes were purchased from Dharmacon (Thermo Fisher Scientific, Waltham, MA, USA) and the lipid based transfection was performed with Lipofectamine-RNAiMAX (Thermo Fisher Scientific, Waltham, MA, USA) using 1 pmol of siRNA per well of a 96-well plate as per the guidelines provided by manufacturer.

    Techniques: Two Tailed Test, RNA Sequencing Assay, Expressing, Functional Assay, Transfection

    FKCU/LTR reporter cells, transfected with the pHook-3/tas plasmid and stained by the β -galassay. Transfection efficiency approximated as 25–30%, based on the development of blue-coloured cells. (Magnification ×200).

    Journal: Journal of Feline Medicine and Surgery

    Article Title: FIP: a novel approach to vaccination

    doi: 10.1016/j.jfms.2003.08.010

    Figure Lengend Snippet: FKCU/LTR reporter cells, transfected with the pHook-3/tas plasmid and stained by the β -galassay. Transfection efficiency approximated as 25–30%, based on the development of blue-coloured cells. (Magnification ×200).

    Article Snippet: A cationic lipid-based transfection system (Lipofectamine, Invitrogen) was used to carry plasmid DNA into FKCU/LTR/zeo/β -gal reporter cells (a kind gift from Prof. A. Rethwilm, University of Wurzburg, Germany).

    Techniques: Transfection, Plasmid Preparation, Staining

    Immunophenotype of primary DCs derived from (A) BALB/c and (B) C57BL6 mice, does not change after nonviral genetic transfection . The 2dDC were transfected with pVR1012-Ag2/PRA plasmid DNA. After 24 h of transfection, the cells were stained with fluorochrome-conjugated antibodies specific to MHC class II, CD11c, CD40, CD80, CD86 cell-surface antigens. The cell debris was gated-out and histogram charts were plotted. The filled histogram charts are of cells stained with isotype-control antibody. The mean fluorescent intensity (MFI) values were determined for the cells under the bar (⊓) region. The values within the charts indicate the MFI values for the nontransfected (NT, black line) and transfected (T, green line) cells stained with antibodies specific to cell-surface antigens. The results are from one representative experiment conducted thrice.

    Journal: BMC Immunology

    Article Title: In vivo trafficking and immunostimulatory potential of an intranasally-administered primary dendritic cell-based vaccine

    doi: 10.1186/1471-2172-11-60

    Figure Lengend Snippet: Immunophenotype of primary DCs derived from (A) BALB/c and (B) C57BL6 mice, does not change after nonviral genetic transfection . The 2dDC were transfected with pVR1012-Ag2/PRA plasmid DNA. After 24 h of transfection, the cells were stained with fluorochrome-conjugated antibodies specific to MHC class II, CD11c, CD40, CD80, CD86 cell-surface antigens. The cell debris was gated-out and histogram charts were plotted. The filled histogram charts are of cells stained with isotype-control antibody. The mean fluorescent intensity (MFI) values were determined for the cells under the bar (⊓) region. The values within the charts indicate the MFI values for the nontransfected (NT, black line) and transfected (T, green line) cells stained with antibodies specific to cell-surface antigens. The results are from one representative experiment conducted thrice.

    Article Snippet: A non-viral, lipid-based transfection reagent: TransIT-TKO (Mirus Bio, WI) was used.

    Techniques: Derivative Assay, Mouse Assay, Transfection, Plasmid Preparation, Staining

    Cytokine (TNF-α, IL-12p70, IL-6 and IL10) levels (pg/ml) in cell-free supernatants of JAWS II and BALB/c mice derived primary DCs after 24 h of genetic transfection with pVR1012-Ag2/PRA plasmid DNA . Results are from two experiments performed in triplicate.

    Journal: BMC Immunology

    Article Title: In vivo trafficking and immunostimulatory potential of an intranasally-administered primary dendritic cell-based vaccine

    doi: 10.1186/1471-2172-11-60

    Figure Lengend Snippet: Cytokine (TNF-α, IL-12p70, IL-6 and IL10) levels (pg/ml) in cell-free supernatants of JAWS II and BALB/c mice derived primary DCs after 24 h of genetic transfection with pVR1012-Ag2/PRA plasmid DNA . Results are from two experiments performed in triplicate.

    Article Snippet: A non-viral, lipid-based transfection reagent: TransIT-TKO (Mirus Bio, WI) was used.

    Techniques: Mouse Assay, Derivative Assay, Transfection, Plasmid Preparation

    (A) Photomicrographs of primary DCs after nonviral genetic transfection . The top panel is a fluorescence photomicrograph of primary DCs showing GFP-expression (in green) after 24 h of transfection. The bottom panel is a photomicrograph of cells in the same field under bright-field filter. (B) Flow cytometric histograms of transfected primary DCs showing the effect of transfection on their viability. The transfected (dotted line) and nontransfected (solid line) primary DCs after two days of culture were stained with propidium iodide. The values indicate % viable nontransfected (NT) and transfected (T) cells under the marked region. The results are from one representative experiment conducted more than three times.

    Journal: BMC Immunology

    Article Title: In vivo trafficking and immunostimulatory potential of an intranasally-administered primary dendritic cell-based vaccine

    doi: 10.1186/1471-2172-11-60

    Figure Lengend Snippet: (A) Photomicrographs of primary DCs after nonviral genetic transfection . The top panel is a fluorescence photomicrograph of primary DCs showing GFP-expression (in green) after 24 h of transfection. The bottom panel is a photomicrograph of cells in the same field under bright-field filter. (B) Flow cytometric histograms of transfected primary DCs showing the effect of transfection on their viability. The transfected (dotted line) and nontransfected (solid line) primary DCs after two days of culture were stained with propidium iodide. The values indicate % viable nontransfected (NT) and transfected (T) cells under the marked region. The results are from one representative experiment conducted more than three times.

    Article Snippet: A non-viral, lipid-based transfection reagent: TransIT-TKO (Mirus Bio, WI) was used.

    Techniques: Transfection, Fluorescence, Expressing, Flow Cytometry, Staining

    The expression of transgenes-encoded Coccidioides -Ag2/PRA and TK antigens by genetically-transfected primary DCs . The cell-lysates were prepared after 24 h of transfection, and dot-immunoblotting was performed with Ag2/PRA and TK-specific antibodies. Left Panel : An Ag2/PRA dot-immunoblot of 5 μg cell-lysate protein from nontransfected (A), transfected primary DCs (B), and 0.2 μg recombinant Ag2/PRA protein (C). Right panel : A TK Dot-immunoblot of 1 μg cell-lysate protein from nontransfected (A) and transfected DCs (B). Results are from one experiment representative of more than three experiments.

    Journal: BMC Immunology

    Article Title: In vivo trafficking and immunostimulatory potential of an intranasally-administered primary dendritic cell-based vaccine

    doi: 10.1186/1471-2172-11-60

    Figure Lengend Snippet: The expression of transgenes-encoded Coccidioides -Ag2/PRA and TK antigens by genetically-transfected primary DCs . The cell-lysates were prepared after 24 h of transfection, and dot-immunoblotting was performed with Ag2/PRA and TK-specific antibodies. Left Panel : An Ag2/PRA dot-immunoblot of 5 μg cell-lysate protein from nontransfected (A), transfected primary DCs (B), and 0.2 μg recombinant Ag2/PRA protein (C). Right panel : A TK Dot-immunoblot of 1 μg cell-lysate protein from nontransfected (A) and transfected DCs (B). Results are from one experiment representative of more than three experiments.

    Article Snippet: A non-viral, lipid-based transfection reagent: TransIT-TKO (Mirus Bio, WI) was used.

    Techniques: Expressing, Transfection, Recombinant

    Gbp4 and Gbp4 KS/AA localize to the speck in the presence of Asc. HEK293T cells were transfected with zebrafish Gbp4-GFP, Gbp4 KS/AA -GFP or Gbp4 ▵CARD -GFP in the presence or absence of zebrafish Asc-Myc, fixed at 48 h post-transfection and labelled in red with specific antibodies to the Myc epitope. ( a ) Representative frontal (xy) and lateral (xz and yz) views of maximum-intensity projection images of HEK293T cells stained with anti-Myc antibodies (Asc, Red). Gbp4, Gbp4 KS/AA and Gbp4 ▵CARD are visualized in green thanks to GFP, while nuclei are labelled with DAPI (blue). ( b ) Quantitation of the percentage of Gbp4 specks in relation to the total number of Gbp4 transfected cells. ( c ) Cells were lysed and anti-GFP and anti-c-Myc antibodies were used to validate the transfection assays by detecting Gbp4 and Asc, respectively, by western blot. The mass weights for all the proteins are indicated. Scale bars, 10 μm. The sample size for each treatment is Gbp4: 1300 cells; Gbp4+Asc: 1270 cells; Gbp4 KS/AA : 369 cells; Gbp4 KS/AA +Asc: 768 cells; Gbp4 ▵CARD : 773 cells; Gbp4 ▵CARD +Asc: 961cells. NS, not significant; *** P

    Journal: Nature Communications

    Article Title: Neutrophils mediate Salmonella Typhimurium clearance through the GBP4 inflammasome-dependent production of prostaglandins

    doi: 10.1038/ncomms12077

    Figure Lengend Snippet: Gbp4 and Gbp4 KS/AA localize to the speck in the presence of Asc. HEK293T cells were transfected with zebrafish Gbp4-GFP, Gbp4 KS/AA -GFP or Gbp4 ▵CARD -GFP in the presence or absence of zebrafish Asc-Myc, fixed at 48 h post-transfection and labelled in red with specific antibodies to the Myc epitope. ( a ) Representative frontal (xy) and lateral (xz and yz) views of maximum-intensity projection images of HEK293T cells stained with anti-Myc antibodies (Asc, Red). Gbp4, Gbp4 KS/AA and Gbp4 ▵CARD are visualized in green thanks to GFP, while nuclei are labelled with DAPI (blue). ( b ) Quantitation of the percentage of Gbp4 specks in relation to the total number of Gbp4 transfected cells. ( c ) Cells were lysed and anti-GFP and anti-c-Myc antibodies were used to validate the transfection assays by detecting Gbp4 and Asc, respectively, by western blot. The mass weights for all the proteins are indicated. Scale bars, 10 μm. The sample size for each treatment is Gbp4: 1300 cells; Gbp4+Asc: 1270 cells; Gbp4 KS/AA : 369 cells; Gbp4 KS/AA +Asc: 768 cells; Gbp4 ▵CARD : 773 cells; Gbp4 ▵CARD +Asc: 961cells. NS, not significant; *** P

    Article Snippet: Transfections were performed with a cationic lipid-based transfection reagent (LyoVec, Invivogen) according to the manufacturer's instructions.

    Techniques: Transfection, Staining, Quantitation Assay, Western Blot

    DNA transfection of neuronal cultures and measured by reporter gene assay Culture cells were transfected with pGL3, BACE1- (551 bp) and hAPP (1.2 kb of upstream regulatory region) reporter plasmids at day 13. Signals were obtained by Luciferase based assay (normalization was done by raw protein OD-595). Results show that expression of both human BACE1 and human APP transfected promoter constructs are easily detectable.

    Journal: Journal of neuroscience methods

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers

    doi: 10.1016/j.jneumeth.2009.08.018

    Figure Lengend Snippet: DNA transfection of neuronal cultures and measured by reporter gene assay Culture cells were transfected with pGL3, BACE1- (551 bp) and hAPP (1.2 kb of upstream regulatory region) reporter plasmids at day 13. Signals were obtained by Luciferase based assay (normalization was done by raw protein OD-595). Results show that expression of both human BACE1 and human APP transfected promoter constructs are easily detectable.

    Article Snippet: A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad).

    Techniques: Transfection, Reporter Gene Assay, Luciferase, Expressing, Construct

    DNA transfection of neuronal culture and visualization by fluorescence microscopy Cells were transfected with pmaxGFP at day 12. Transfection was carried out by lipid based transfection reagent (Transfectin ® ; Bio-Rad). Transfected cells started expressing ‘green fluorescent protein’ approximately after 36 hours of transfection and maximum expression was noticed after 48 hours. Percentage calculation after manual counting of green transfected cells and non-transfected cells revealed an approximate transfection efficiency of 25-30%.

    Journal: Journal of neuroscience methods

    Article Title: Molecular and immunocytochemical characterization of primary neuronal cultures from adult rat brain: differential expression of neuronal and glial protein markers

    doi: 10.1016/j.jneumeth.2009.08.018

    Figure Lengend Snippet: DNA transfection of neuronal culture and visualization by fluorescence microscopy Cells were transfected with pmaxGFP at day 12. Transfection was carried out by lipid based transfection reagent (Transfectin ® ; Bio-Rad). Transfected cells started expressing ‘green fluorescent protein’ approximately after 36 hours of transfection and maximum expression was noticed after 48 hours. Percentage calculation after manual counting of green transfected cells and non-transfected cells revealed an approximate transfection efficiency of 25-30%.

    Article Snippet: A plasmid containing a green fluorescent protein gene under the control of the cytomegalovirus promoter (pmaxGFP, Amaxa, Walkersville, MD, USA) was transfected into the cells at day 12 using lipid-based transfection reagent (Transfectin® , Bio-Rad).

    Techniques: Transfection, Fluorescence, Microscopy, Expressing