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  • 99
    Thermo Fisher lipid based transfection agent siport neofx
    Effect of mimitin gene silencing on proliferation of HepG2 cells . Cells were transfected with silencing RNA specific for mimitin (50 nM), or for GAPDH (100 nM, positive control), or unspecific siRNA (negative control, 50 nM) or were treated with <t>transfection</t> reagent <t>siPORT</t> <t>NeoFX</t> alone (blank). At 72 h post transfection cells were incubated with BrdU (5 μM) for 3 h and incorporation was determined with Cell Proliferation ELISA. A. Cell proliferation is shown relative to the non-sepcific siRNA (control). Values were calculated from absorbance readings at 450 nm and represent the mean of three independent experiments with standard deviation (*p
    Lipid Based Transfection Agent Siport Neofx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection agent siport neofx/product/Thermo Fisher
    Average 99 stars, based on 630 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection agent siport neofx - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    93
    Santa Cruz Biotechnology lipid based transfection agent
    Effect of mimitin gene silencing on proliferation of HepG2 cells . Cells were transfected with silencing RNA specific for mimitin (50 nM), or for GAPDH (100 nM, positive control), or unspecific siRNA (negative control, 50 nM) or were treated with <t>transfection</t> reagent <t>siPORT</t> <t>NeoFX</t> alone (blank). At 72 h post transfection cells were incubated with BrdU (5 μM) for 3 h and incorporation was determined with Cell Proliferation ELISA. A. Cell proliferation is shown relative to the non-sepcific siRNA (control). Values were calculated from absorbance readings at 450 nm and represent the mean of three independent experiments with standard deviation (*p
    Lipid Based Transfection Agent, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection agent/product/Santa Cruz Biotechnology
    Average 93 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection agent - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    92
    Thermo Fisher lipid based siportneofx agent
    Effect of mimitin gene silencing on proliferation of HepG2 cells . Cells were transfected with silencing RNA specific for mimitin (50 nM), or for GAPDH (100 nM, positive control), or unspecific siRNA (negative control, 50 nM) or were treated with <t>transfection</t> reagent <t>siPORT</t> <t>NeoFX</t> alone (blank). At 72 h post transfection cells were incubated with BrdU (5 μM) for 3 h and incorporation was determined with Cell Proliferation ELISA. A. Cell proliferation is shown relative to the non-sepcific siRNA (control). Values were calculated from absorbance readings at 450 nm and represent the mean of three independent experiments with standard deviation (*p
    Lipid Based Siportneofx Agent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based siportneofx agent/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lipid based siportneofx agent - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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    Effect of mimitin gene silencing on proliferation of HepG2 cells . Cells were transfected with silencing RNA specific for mimitin (50 nM), or for GAPDH (100 nM, positive control), or unspecific siRNA (negative control, 50 nM) or were treated with transfection reagent siPORT NeoFX alone (blank). At 72 h post transfection cells were incubated with BrdU (5 μM) for 3 h and incorporation was determined with Cell Proliferation ELISA. A. Cell proliferation is shown relative to the non-sepcific siRNA (control). Values were calculated from absorbance readings at 450 nm and represent the mean of three independent experiments with standard deviation (*p

    Journal: BMC Cell Biology

    Article Title: Mimitin - a novel cytokine-regulated mitochondrial protein

    doi: 10.1186/1471-2121-10-23

    Figure Lengend Snippet: Effect of mimitin gene silencing on proliferation of HepG2 cells . Cells were transfected with silencing RNA specific for mimitin (50 nM), or for GAPDH (100 nM, positive control), or unspecific siRNA (negative control, 50 nM) or were treated with transfection reagent siPORT NeoFX alone (blank). At 72 h post transfection cells were incubated with BrdU (5 μM) for 3 h and incorporation was determined with Cell Proliferation ELISA. A. Cell proliferation is shown relative to the non-sepcific siRNA (control). Values were calculated from absorbance readings at 450 nm and represent the mean of three independent experiments with standard deviation (*p

    Article Snippet: Lipid-based transfection reagent siPORT NeoFX (4 μl/well) from Ambion and siRNAs (50 nM final concentration) were separately diluted in 50 μl OPTIMEM and then mixed together.

    Techniques: Transfection, Positive Control, Negative Control, Incubation, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Effect of mimitin gene silencing on caspase 3/7 activities . HepG2 cells were transfected with mimitin-siRNA, GAPDH-siRNA, negative control siRNA, or transfection reagent (siPORT NeoFX) (blank). Apoptosis was induced 72 h post transfection by treatment of cells with TNF/CHX for 5 h. Caspase 3/7 activity was measured using Caspase-GloTM3/7 assay kit. A. Changes in caspase activity in cells with TNF/CHX-induced apoptosis were compared to cells treated with TNF/CHX and transfected with a negative control siRNA (assumed as equal 1). In each case the value of caspase 3/7 activity after TNF/CHX treatment was divided by the value of basal activity of non-stimulated cells. Values represent the mean of three independent experiments with error bars showing standard error (* p

    Journal: BMC Cell Biology

    Article Title: Mimitin - a novel cytokine-regulated mitochondrial protein

    doi: 10.1186/1471-2121-10-23

    Figure Lengend Snippet: Effect of mimitin gene silencing on caspase 3/7 activities . HepG2 cells were transfected with mimitin-siRNA, GAPDH-siRNA, negative control siRNA, or transfection reagent (siPORT NeoFX) (blank). Apoptosis was induced 72 h post transfection by treatment of cells with TNF/CHX for 5 h. Caspase 3/7 activity was measured using Caspase-GloTM3/7 assay kit. A. Changes in caspase activity in cells with TNF/CHX-induced apoptosis were compared to cells treated with TNF/CHX and transfected with a negative control siRNA (assumed as equal 1). In each case the value of caspase 3/7 activity after TNF/CHX treatment was divided by the value of basal activity of non-stimulated cells. Values represent the mean of three independent experiments with error bars showing standard error (* p

    Article Snippet: Lipid-based transfection reagent siPORT NeoFX (4 μl/well) from Ambion and siRNAs (50 nM final concentration) were separately diluted in 50 μl OPTIMEM and then mixed together.

    Techniques: Transfection, Negative Control, Activity Assay