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  • 99
    Thermo Fisher hybrid linear quadrupole ion trap orbitrap mass spectrometer
    (a) CID on the <t>LTQ-Orbitrap</t> of the crosslinked homodimer synthetic peptide (Ac-QIGKGVAR) results in the same two major cleavage events observed in MALDI at the intrinsic positive charges. (b) MS 3 of the fragment without an intrinsic positive charge produces
    Hybrid Linear Quadrupole Ion Trap Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid linear quadrupole ion trap orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 114 article reviews
    Price from $9.99 to $1999.99
    hybrid linear quadrupole ion trap orbitrap mass spectrometer - by Bioz Stars, 2020-08
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    88
    Thermo Fisher linear quadrupole ion trap orbitrap ltq orbitrap mass spectrometer
    MS/MS profile of ion m / z 601.41. A tandem mass spectrum of a prevalent ion on a particular time point in the LC gradient and ionized on the <t>LTQ-Orbitrap</t> is shown. The peptide fragments randomly on each amide bond, resulting in carboxyl-terminal y ions or amino-terminal b ions. After the fragment masses were submitted to Mascot, the peptide was identified as FGDQVVAVLTR ( inset with detected y and b ions represented) from protein Rv3220c, a probable two-component sensor kinase. * Asterisk represent the parent ion.
    Linear Quadrupole Ion Trap Orbitrap Ltq Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/linear quadrupole ion trap orbitrap ltq orbitrap mass spectrometer/product/Thermo Fisher
    Average 88 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    linear quadrupole ion trap orbitrap ltq orbitrap mass spectrometer - by Bioz Stars, 2020-08
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    85
    Thermo Fisher liner ion trap mass spectrometer linear trap quadrupole ltq orbitrap
    MS/MS profile of ion m / z 601.41. A tandem mass spectrum of a prevalent ion on a particular time point in the LC gradient and ionized on the <t>LTQ-Orbitrap</t> is shown. The peptide fragments randomly on each amide bond, resulting in carboxyl-terminal y ions or amino-terminal b ions. After the fragment masses were submitted to Mascot, the peptide was identified as FGDQVVAVLTR ( inset with detected y and b ions represented) from protein Rv3220c, a probable two-component sensor kinase. * Asterisk represent the parent ion.
    Liner Ion Trap Mass Spectrometer Linear Trap Quadrupole Ltq Orbitrap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 6 article reviews
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    91
    Thermo Fisher hybrid dual cell quadrupole linear ion trap orbitrap mass spectrometer ltq orbitrap elite
    MS/MS profile of ion m / z 601.41. A tandem mass spectrum of a prevalent ion on a particular time point in the LC gradient and ionized on the <t>LTQ-Orbitrap</t> is shown. The peptide fragments randomly on each amide bond, resulting in carboxyl-terminal y ions or amino-terminal b ions. After the fragment masses were submitted to Mascot, the peptide was identified as FGDQVVAVLTR ( inset with detected y and b ions represented) from protein Rv3220c, a probable two-component sensor kinase. * Asterisk represent the parent ion.
    Hybrid Dual Cell Quadrupole Linear Ion Trap Orbitrap Mass Spectrometer Ltq Orbitrap Elite, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid dual cell quadrupole linear ion trap orbitrap mass spectrometer ltq orbitrap elite/product/Thermo Fisher
    Average 91 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    hybrid dual cell quadrupole linear ion trap orbitrap mass spectrometer ltq orbitrap elite - by Bioz Stars, 2020-08
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    99
    Thermo Fisher quadrupole ion trap mass spectrometer
    MS/MS profile of ion m / z 601.41. A tandem mass spectrum of a prevalent ion on a particular time point in the LC gradient and ionized on the <t>LTQ-Orbitrap</t> is shown. The peptide fragments randomly on each amide bond, resulting in carboxyl-terminal y ions or amino-terminal b ions. After the fragment masses were submitted to Mascot, the peptide was identified as FGDQVVAVLTR ( inset with detected y and b ions represented) from protein Rv3220c, a probable two-component sensor kinase. * Asterisk represent the parent ion.
    Quadrupole Ion Trap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quadrupole ion trap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 149 article reviews
    Price from $9.99 to $1999.99
    quadrupole ion trap mass spectrometer - by Bioz Stars, 2020-08
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    88
    Thermo Fisher linear quadrupole ion trap orbitrap
    MS/MS profile of ion m / z 601.41. A tandem mass spectrum of a prevalent ion on a particular time point in the LC gradient and ionized on the <t>LTQ-Orbitrap</t> is shown. The peptide fragments randomly on each amide bond, resulting in carboxyl-terminal y ions or amino-terminal b ions. After the fragment masses were submitted to Mascot, the peptide was identified as FGDQVVAVLTR ( inset with detected y and b ions represented) from protein Rv3220c, a probable two-component sensor kinase. * Asterisk represent the parent ion.
    Linear Quadrupole Ion Trap Orbitrap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/linear quadrupole ion trap orbitrap/product/Thermo Fisher
    Average 88 stars, based on 170 article reviews
    Price from $9.99 to $1999.99
    linear quadrupole ion trap orbitrap - by Bioz Stars, 2020-08
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    Image Search Results


    (a) CID on the LTQ-Orbitrap of the crosslinked homodimer synthetic peptide (Ac-QIGKGVAR) results in the same two major cleavage events observed in MALDI at the intrinsic positive charges. (b) MS 3 of the fragment without an intrinsic positive charge produces

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Quaternary Diamines as Mass Spectrometry Cleavable Crosslinkers for Protein Interactions

    doi: 10.1007/s13361-011-0288-4

    Figure Lengend Snippet: (a) CID on the LTQ-Orbitrap of the crosslinked homodimer synthetic peptide (Ac-QIGKGVAR) results in the same two major cleavage events observed in MALDI at the intrinsic positive charges. (b) MS 3 of the fragment without an intrinsic positive charge produces

    Article Snippet: The crosslinked standard peptides were analyzed with a hybrid linear quadrupole ion trap-Orbitrap mass spectrometer (ThermoFisher Scientific, Inc. model LTQ-Orbitrap XL; San Jose, CA, USA).

    Techniques: Mass Spectrometry

    Stem cell therapy-specific subproteome. ( A ): Comparison of stem cell-treated [ES(+)] versus untreated [ES(−)] left ventricular tissue extracts by two-dimensional (2D) electrophoresis. Differentially expressed spots isolated for identification by LTQ-Orbitrap mass spectrometric analysis are circled, and numbered on the ES(+) gel. Inset: Gel-to-gel reproducibility indicated by correlation of scatter plot for average normalized densitometric intensities of matching protein spots from ES(+) versus ES(−) gels. ( B ): Identities of the 61 proteins significantly altered by cell therapy are listed with their symbol (Swiss-Prot gene abbreviation) and spot numbers to locate corresponding 2D gel position(s) in panel ( A ). Mascot score, number of unique identified peptides, % sequence cov. (coverage), predicted M r and p I for each protein (following expected post-translational processing, for example, removal of a mitochondrial signal peptide), and fold change in ES(+) versus ES(−) are indicated. For proteins detected in more than one spot, maximum score and corresponding number of unique peptides are reported. Fold change was calculated as described in experimental procedures, and for proteins detected in both increasing and decreasing spots (*), both values are indicated. Abbreviations: ES, embryonic stem cells; Ox., oxidative; TCA cycle, tricarboxylic acid cycle; SAPK, stress activated protein kinase.

    Journal: Stem cells (Dayton, Ohio)

    Article Title: ATP-Sensitive K+ Channel-Deficient Dilated Cardiomyopathy Proteome Remodeled by Embryonic Stem Cell Therapy

    doi: 10.1002/stem.465

    Figure Lengend Snippet: Stem cell therapy-specific subproteome. ( A ): Comparison of stem cell-treated [ES(+)] versus untreated [ES(−)] left ventricular tissue extracts by two-dimensional (2D) electrophoresis. Differentially expressed spots isolated for identification by LTQ-Orbitrap mass spectrometric analysis are circled, and numbered on the ES(+) gel. Inset: Gel-to-gel reproducibility indicated by correlation of scatter plot for average normalized densitometric intensities of matching protein spots from ES(+) versus ES(−) gels. ( B ): Identities of the 61 proteins significantly altered by cell therapy are listed with their symbol (Swiss-Prot gene abbreviation) and spot numbers to locate corresponding 2D gel position(s) in panel ( A ). Mascot score, number of unique identified peptides, % sequence cov. (coverage), predicted M r and p I for each protein (following expected post-translational processing, for example, removal of a mitochondrial signal peptide), and fold change in ES(+) versus ES(−) are indicated. For proteins detected in more than one spot, maximum score and corresponding number of unique peptides are reported. Fold change was calculated as described in experimental procedures, and for proteins detected in both increasing and decreasing spots (*), both values are indicated. Abbreviations: ES, embryonic stem cells; Ox., oxidative; TCA cycle, tricarboxylic acid cycle; SAPK, stress activated protein kinase.

    Article Snippet: Chromatography was performed using 0.2% formic acid in solvent A (99% water, 1% acetonitrile) and B (80% acetonitrile, 5% isopropanol, 15% water), with peptides eluted over 30 minutes with a 5%–45% solvent B gradient using an Eksigent nanoHPLC system (MDS Sciex, Toronto, Canada) coupled to a linear ion trap quadrupole (LTQ)-Orbitrap mass spectrometer (Thermo Fisher Scientific, Barrington, IL).

    Techniques: Two-Dimensional Gel Electrophoresis, Isolation, Sequencing

    MS/MS profile of ion m / z 601.41. A tandem mass spectrum of a prevalent ion on a particular time point in the LC gradient and ionized on the LTQ-Orbitrap is shown. The peptide fragments randomly on each amide bond, resulting in carboxyl-terminal y ions or amino-terminal b ions. After the fragment masses were submitted to Mascot, the peptide was identified as FGDQVVAVLTR ( inset with detected y and b ions represented) from protein Rv3220c, a probable two-component sensor kinase. * Asterisk represent the parent ion.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Using a Label-free Proteomics Method to Identify Differentially Abundant Proteins in Closely Related Hypo- and Hypervirulent Clinical Mycobacterium tuberculosis Beijing Isolates *

    doi: 10.1074/mcp.M900422-MCP200

    Figure Lengend Snippet: MS/MS profile of ion m / z 601.41. A tandem mass spectrum of a prevalent ion on a particular time point in the LC gradient and ionized on the LTQ-Orbitrap is shown. The peptide fragments randomly on each amide bond, resulting in carboxyl-terminal y ions or amino-terminal b ions. After the fragment masses were submitted to Mascot, the peptide was identified as FGDQVVAVLTR ( inset with detected y and b ions represented) from protein Rv3220c, a probable two-component sensor kinase. * Asterisk represent the parent ion.

    Article Snippet: Mass Spectrometry All experiments were performed on a Dionex Ultimate 3000 nano-LC system (Dionex, Sunnyvale, CA) connected to a linear quadrupole ion trap-Orbitrap (LTQ-Orbitrap) mass spectrometer (Thermo Electron, Bremen, Germany) equipped with a nanoelectrospray ion source.

    Techniques: Mass Spectrometry

    Workflow to identify lipid raft-associated proteins. Stable isotopic labeling of amino acids in cell culture (SILAC) labeled SH-SY5Y cells left untreated or exposed to 400 nM okadaic acid for 50 min before mixed in a ratio 1:1 in 0.7% Triton X-100/MES buffer. After centrifugation in a sucrose gradient, 10 fractions of 0.5 mL were collected. The lipid raft fractions were identified by Western blotting using flotillin as a lipid raft marker. Fractions containing flotillin were pooled prior to removal of lipids by chloroform/methanol extraction, followed by acetone precipitation. Proteins were separated by SDS-PAGE. Proteins were in-gel digested with trypsin, and peptides were purified either with (1) C18 or (2) IMAC/TiO 2 and analyzed on LTQ-Orbitrap. Proteins were identified with Mascot (ver. 2.2) and quantified using MaxQuant (ver. 1.13).

    Journal: Marine Drugs

    Article Title: Identification of Dynamic Changes in Proteins Associated with the Cellular Cytoskeleton after Exposure to Okadaic Acid

    doi: 10.3390/md11061763

    Figure Lengend Snippet: Workflow to identify lipid raft-associated proteins. Stable isotopic labeling of amino acids in cell culture (SILAC) labeled SH-SY5Y cells left untreated or exposed to 400 nM okadaic acid for 50 min before mixed in a ratio 1:1 in 0.7% Triton X-100/MES buffer. After centrifugation in a sucrose gradient, 10 fractions of 0.5 mL were collected. The lipid raft fractions were identified by Western blotting using flotillin as a lipid raft marker. Fractions containing flotillin were pooled prior to removal of lipids by chloroform/methanol extraction, followed by acetone precipitation. Proteins were separated by SDS-PAGE. Proteins were in-gel digested with trypsin, and peptides were purified either with (1) C18 or (2) IMAC/TiO 2 and analyzed on LTQ-Orbitrap. Proteins were identified with Mascot (ver. 2.2) and quantified using MaxQuant (ver. 1.13).

    Article Snippet: For general lipid raft proteome mapping, the peptides were separated on a Dionex Ultimate 3000 nano-LC system (Sunnyvale, CA, USA) coupled to a linear quadrupole ion trap (LTQ-Orbitrap) mass spectrometer (Thermo Scientific).

    Techniques: Isotopic Labeling, Cell Culture, Labeling, Centrifugation, Western Blot, Marker, SDS Page, Purification