lightcycler→ 480 sybr green i master mix Roche Search Results


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  • 82
    Roche lightcycle 480 sybr green i master mix
    Lightcycle 480 Sybr Green I Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 82/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Roche lightcycler 480 sybr green i master mix
    Lightcycler 480 Sybr Green I Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 5180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Roche lightcycler 480 sybr green i master mix kit
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Sybr Green I Master Mix Kit, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Roche lightcycler 480 sybr green i master mix reagent
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Sybr Green I Master Mix Reagent, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler 480 sybr green i master reaction mix
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Sybr Green I Master Reaction Mix, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Roche lightcycler 480 sybr green i master mix system
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Sybr Green I Master Mix System, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler 480 sybr green i master enzyme mix
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Sybr Green I Master Enzyme Mix, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler 480 sybr green i master mix chemistry
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Sybr Green I Master Mix Chemistry, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler 480 dna sybr green i master mix
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Dna Sybr Green I Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler 480 sybr green i master qpcr mix
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Sybr Green I Master Qpcr Mix, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler 480 sybr green i master pcr mix
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Sybr Green I Master Pcr Mix, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler 480 dna sybr green i master reaction mix
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Dna Sybr Green I Master Reaction Mix, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler 480 sybr green i master qpcr reaction mixture
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Sybr Green I Master Qpcr Reaction Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler 480 sybr green i master buffer
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    Lightcycler 480 Sybr Green I Master Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 sybr green i master buffer/product/Roche
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    Roche 2x lightcycler 480 sybr green i master
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    2x Lightcycler 480 Sybr Green I Master, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2x lightcycler 480 sybr green i master/product/Roche
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    84
    Roche 2×lightcycler 480 sybr green i master
    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche <t>LightCycler</t> 480 and LightCycler 480 <t>SYBR</t> <t>Green</t> I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p
    2×Lightcycler 480 Sybr Green I Master, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche LightCycler 480 and LightCycler 480 SYBR Green I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p

    Journal: The Biochemical journal

    Article Title: Regulation of differential proton-coupled folate transporter gene expression in human tumors: transactivation by KLF15 with NRF-1 and the role of Sp1

    doi: 10.1042/BCJ20180394

    Figure Lengend Snippet: Binding determinations of NRF-1, KLF15 and Sp1 transcription factors to the hPCFT minimal promoter region by ChIP and EMSA assays. ( A ) ChIP assays were performed in HepG2 and HT1080 cells according to the manufacturer’s instructions, with antibodies against NRF-1, KLF15, and Sp1. Mouse IgG2a was used as a negative control in the pull-down step. The ChIP DNA and input DNA were prepared with the ChIP-IT High Sensitivity Kit according to the manufacturer’s instructions and were then used for real-time PCR analyses with the ‘DNA Standards, Design and Analysis Template’ provided with the ChIP-IT qPCR Analysis kit (Active Motif). Real-time PCR was performed using a Roche LightCycler 480 and LightCycler 480 SYBR Green I Master kit, with primers located in the hPCFT core promoter region spanning putative binding sites for NRF-1, KLF15 or Sp1. Statistical analyses were performed using Prism 6.07. Results are presented as mean values ± standard errors from at least three independent experiments of triplicate measurements. **** p

    Article Snippet: The qPCRs were performed using a Roche LightCycler 480 and LightCycler 480 SYBR Green I Master Kit Mix (Roche Diagnostics), with primers located in the hPCFT core promoter region that includes putative binding sites for NRF-1, KLF15 or Sp1.

    Techniques: Binding Assay, Chromatin Immunoprecipitation, Negative Control, Real-time Polymerase Chain Reaction, SYBR Green Assay

    NRF-1, KLF15, and Sp1 expression in HepG2 and HT1080 cells and the impact of NRF-1, KLF15 and Sp1 on hPCFT gene expression. ( A ) Transcript levels for KLF15, NRF-1 and Sp1 were measured in HepG2 and HT1080 cell lines. ( B ) Impact of hPCFT gene expression in HepG2 cells stably transfected with KLF15, NRF-1 or Sp1. ( C ) Impact of hPCFT gene expression in HT1080 cells stably transfected with KLF15, NRF-1 or Sp1. ( D ) Impact of hPCFT gene expression in HT1080 cells transiently transfected with KLF15, NRF-1 or Sp1 singly or in combination. Transcript levels of NRF-1, KLF15, Sp1 and hPCFT were monitored by real-time RT-PCR with a LightCycler 480 Probes Master kit, or with a LightCycler 480 SYBR Green I Master kit. The transcript levels were normalized to that for GAPDH and/or β-actin. Results are presented as mean values ± standard errors from three to four different experiments. For statistics: *** p

    Journal: The Biochemical journal

    Article Title: Regulation of differential proton-coupled folate transporter gene expression in human tumors: transactivation by KLF15 with NRF-1 and the role of Sp1

    doi: 10.1042/BCJ20180394

    Figure Lengend Snippet: NRF-1, KLF15, and Sp1 expression in HepG2 and HT1080 cells and the impact of NRF-1, KLF15 and Sp1 on hPCFT gene expression. ( A ) Transcript levels for KLF15, NRF-1 and Sp1 were measured in HepG2 and HT1080 cell lines. ( B ) Impact of hPCFT gene expression in HepG2 cells stably transfected with KLF15, NRF-1 or Sp1. ( C ) Impact of hPCFT gene expression in HT1080 cells stably transfected with KLF15, NRF-1 or Sp1. ( D ) Impact of hPCFT gene expression in HT1080 cells transiently transfected with KLF15, NRF-1 or Sp1 singly or in combination. Transcript levels of NRF-1, KLF15, Sp1 and hPCFT were monitored by real-time RT-PCR with a LightCycler 480 Probes Master kit, or with a LightCycler 480 SYBR Green I Master kit. The transcript levels were normalized to that for GAPDH and/or β-actin. Results are presented as mean values ± standard errors from three to four different experiments. For statistics: *** p

    Article Snippet: The qPCRs were performed using a Roche LightCycler 480 and LightCycler 480 SYBR Green I Master Kit Mix (Roche Diagnostics), with primers located in the hPCFT core promoter region that includes putative binding sites for NRF-1, KLF15 or Sp1.

    Techniques: Expressing, Stable Transfection, Transfection, Quantitative RT-PCR, SYBR Green Assay

    Expression and regulatory associations between hPCFT and NRF-1, Sp1, or KLF15 in solid tumor cell lines. ) by real-time RT-PCR with LightCycler 480 SYBR Green I Master kit. The hPCFT, NRF-1, Sp1, and KLF15 transcript levels were normalized to transcript levels for GAPDH. The scatter plots show univariate associations between hPCFT and NRF-1 ( A ), hPCFT and Sp1 ( B ), and hPCFT and KLF15 ( C ) by Pearson’s correlation coefficients ( r ). The x-axis and y-axis scales are logarithmic and the solid gray lines represent the fitted regression lines. N indicates the sample size (53). ( D ]. For the schematic shown, the thickness of the edge and the corresponding numbers (which range from 0 to 1) represent the relative strength of the association. The R/Bioconductor packages minet and igraph ].

    Journal: The Biochemical journal

    Article Title: Regulation of differential proton-coupled folate transporter gene expression in human tumors: transactivation by KLF15 with NRF-1 and the role of Sp1

    doi: 10.1042/BCJ20180394

    Figure Lengend Snippet: Expression and regulatory associations between hPCFT and NRF-1, Sp1, or KLF15 in solid tumor cell lines. ) by real-time RT-PCR with LightCycler 480 SYBR Green I Master kit. The hPCFT, NRF-1, Sp1, and KLF15 transcript levels were normalized to transcript levels for GAPDH. The scatter plots show univariate associations between hPCFT and NRF-1 ( A ), hPCFT and Sp1 ( B ), and hPCFT and KLF15 ( C ) by Pearson’s correlation coefficients ( r ). The x-axis and y-axis scales are logarithmic and the solid gray lines represent the fitted regression lines. N indicates the sample size (53). ( D ]. For the schematic shown, the thickness of the edge and the corresponding numbers (which range from 0 to 1) represent the relative strength of the association. The R/Bioconductor packages minet and igraph ].

    Article Snippet: The qPCRs were performed using a Roche LightCycler 480 and LightCycler 480 SYBR Green I Master Kit Mix (Roche Diagnostics), with primers located in the hPCFT core promoter region that includes putative binding sites for NRF-1, KLF15 or Sp1.

    Techniques: Expressing, Quantitative RT-PCR, SYBR Green Assay