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  • 97
    Kapa Biosystems library quantification kit illumina lightcycler 480
    Library Quantification Kit Illumina Lightcycler 480, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 97/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad lightcycler
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Lightcycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher lightcycler
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Lightcycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Kapa Biosystems kapa sybr fast roche lightcycler 480 2x qpcr master mix
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Kapa Sybr Fast Roche Lightcycler 480 2x Qpcr Master Mix, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher lightcycler 480 system
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Lightcycler 480 System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad lightcycler 480
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Lightcycler 480, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Roche roche lightcycler 480
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Roche Lightcycler 480, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 12167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Kapa Biosystems lightcycler 480
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Lightcycler 480, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 94/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Kapa Biosystems kapa library quantification kit
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Kapa Library Quantification Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 94/100, based on 7233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    thermo fisher lightcycler 480 sybr green i master mix
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Lightcycler 480 Sybr Green I Master Mix, supplied by thermo fisher, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher lightcycler 480 real time pcr system
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Lightcycler 480 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen quantitect sybr green pcr lightcycler kit
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Quantitect Sybr Green Pcr Lightcycler Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Stratagene lightcycler quantitative pcr apparatus
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Lightcycler Quantitative Pcr Apparatus, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Bio-Rad bio rad iq5 lightcycler
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Bio Rad Iq5 Lightcycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Bio-Rad lightcycler cfx96
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Lightcycler Cfx96, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Bio-Rad lightcycler iq 5
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Lightcycler Iq 5, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies lightcycler 1536 multiwell plates
    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a <t>LightCycler.</t> Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.
    Lightcycler 1536 Multiwell Plates, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a LightCycler. Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Targeting of mTOR catalytic site inhibits multiple steps of the HIV-1 lifecycle and suppresses HIV-1 viremia in humanized mice

    doi: 10.1073/pnas.1511144112

    Figure Lengend Snippet: INK128 inhibits HIV transcription in U1 cells. U1 cells were cultured in the presence of 10 nM PMA and the indicated concentrations of INK128. After 2 d, cells were collected, mRNA isolated, quantified, reverse transcribed and amplified by real time PCR using unspliced HIV cDNA primer pair US.1a/US.2a and housekeeping [beta]-actin primers. For quantification, standard curves for unspliced HIV cDNA and [beta]-actin sequences were generated by performing 10-fold serial dilutions of mRNA isolated from PBLs acutely infected with HIV BaL. PCR amplification was performed using Quantitect SYBR Green PCR Kit in a LightCycler. Negative controls consisted of mixture reactions without the reverse transcription step. Data are means ± SD of two experiments, expressed as relative HIV mRNA expression compared with cultures containing no INK128 after normalization to [beta]-actin levels.

    Article Snippet: PCR amplification was performed using Quantitect SYBR Green PCR Kit (Qiagen) in a LightCycler (Biorad).

    Techniques: Cell Culture, Isolation, Amplification, Real-time Polymerase Chain Reaction, Generated, Infection, Polymerase Chain Reaction, SYBR Green Assay, Expressing