lightcycler 480 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Roche lightcycler 480
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 32920 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480/product/Roche
    Average 99 stars, based on 32920 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    91
    Kapa Biosystems lightcycler 480
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 91/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480/product/Kapa Biosystems
    Average 91 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    91
    Roche lightcycle 480
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycle 480, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycle 480/product/Roche
    Average 91 stars, based on 122 article reviews
    Price from $9.99 to $1999.99
    lightcycle 480 - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    93
    Bio-Rad lightcycler 480
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480/product/Bio-Rad
    Average 93 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    93
    Thermo Fisher lightcycler 480
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480/product/Thermo Fisher
    Average 93 stars, based on 112 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    79
    Roche lightcycler 480 instrumentlightcycler 480
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480 Instrumentlightcycler 480, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 instrumentlightcycler 480/product/Roche
    Average 79 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 instrumentlightcycler 480 - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    87
    Roche lightcycler lightcycler 480 system
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler Lightcycler 480 System, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler lightcycler 480 system/product/Roche
    Average 87 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    lightcycler lightcycler 480 system - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    99
    Roche lightcycler 480 apparatus
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480 Apparatus, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 apparatus/product/Roche
    Average 99 stars, based on 650 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 apparatus - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    99
    Roche lightcycler 480 machine
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480 Machine, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 825 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 machine/product/Roche
    Average 99 stars, based on 825 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 machine - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    93
    BIOplastics roche lightcycler 480
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Roche Lightcycler 480, supplied by BIOplastics, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/roche lightcycler 480/product/BIOplastics
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    roche lightcycler 480 - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    99
    Roche lightcycler 480 thermocycler
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480 Thermocycler, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 548 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 thermocycler/product/Roche
    Average 99 stars, based on 548 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 thermocycler - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    87
    Roche lightcycle 480 system
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycle 480 System, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycle 480 system/product/Roche
    Average 87 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    lightcycle 480 system - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    99
    Roche lightcycler 480 platform
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480 Platform, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 platform/product/Roche
    Average 99 stars, based on 484 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 platform - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    77
    Roche lightcycler 480 protocol
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480 Protocol, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 protocol/product/Roche
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 protocol - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    83
    Roche lightcycle 480 thermocycler
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycle 480 Thermocycler, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycle 480 thermocycler/product/Roche
    Average 83 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    lightcycle 480 thermocycler - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    76
    Roche lightcycler 480 iimachine
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480 Iimachine, supplied by Roche, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 iimachine/product/Roche
    Average 76 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 iimachine - by Bioz Stars, 2020-02
    76/100 stars
      Buy from Supplier

    90
    Roche lightcycler 480 sybrgreen
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480 Sybrgreen, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 sybrgreen/product/Roche
    Average 90 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 sybrgreen - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    77
    Roche lightcycler 480 cycler
    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.
    Lightcycler 480 Cycler, supplied by Roche, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 cycler/product/Roche
    Average 77 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 cycler - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    78
    Roche lightcycler 480 taqman
    Real-time PCR analysis of microphthalmia-associated transcription factor (MITF) and Paired box 3 (PAX3) in human epidermal melanocytes treated with 3,6-dihydroxy-2,4-dimethoxy-dibenzofuran ( 9 ) and 3,4-dihydroxy-5-methoxybiphenyl-2′- O -β- d -glucopyranoside ( 13 ). Cells (1 × 10 6 ) were treated with different concentrations (100, 80, and 60 µM) of compounds 9 and 13 for 24 h. Quantification of gene transcripts was performed using a LightCycler ® 480 <t>TaqMan</t> according to the manufacturer’s instructions. Findings were normalized to the expression of GAPDH mRNA. Measurements were conducted in triplicate, and mean expression values for test samples relative to mean expression values for negative controls are indicated. C: control, A: arbutin (2.5 mM), C9: 3,6-dihydroxy-2,4-dimethoxy-dibenzofuran ( 9 ), C13: 3,4-dihydroxy-5-methoxybiphenyl-2′- O -β- d -glucopyranoside ( 13 ). Differences in data were evaluated for statistical significance (** p
    Lightcycler 480 Taqman, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 taqman/product/Roche
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 taqman - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    93
    Bio-Rad lightcycler 480 system
    Real-time PCR analysis of microphthalmia-associated transcription factor (MITF) and Paired box 3 (PAX3) in human epidermal melanocytes treated with 3,6-dihydroxy-2,4-dimethoxy-dibenzofuran ( 9 ) and 3,4-dihydroxy-5-methoxybiphenyl-2′- O -β- d -glucopyranoside ( 13 ). Cells (1 × 10 6 ) were treated with different concentrations (100, 80, and 60 µM) of compounds 9 and 13 for 24 h. Quantification of gene transcripts was performed using a LightCycler ® 480 <t>TaqMan</t> according to the manufacturer’s instructions. Findings were normalized to the expression of GAPDH mRNA. Measurements were conducted in triplicate, and mean expression values for test samples relative to mean expression values for negative controls are indicated. C: control, A: arbutin (2.5 mM), C9: 3,6-dihydroxy-2,4-dimethoxy-dibenzofuran ( 9 ), C13: 3,4-dihydroxy-5-methoxybiphenyl-2′- O -β- d -glucopyranoside ( 13 ). Differences in data were evaluated for statistical significance (** p
    Lightcycler 480 System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 system/product/Bio-Rad
    Average 93 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 system - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    88
    Roche lightcycler 480 plates
    Real-time PCR analysis of microphthalmia-associated transcription factor (MITF) and Paired box 3 (PAX3) in human epidermal melanocytes treated with 3,6-dihydroxy-2,4-dimethoxy-dibenzofuran ( 9 ) and 3,4-dihydroxy-5-methoxybiphenyl-2′- O -β- d -glucopyranoside ( 13 ). Cells (1 × 10 6 ) were treated with different concentrations (100, 80, and 60 µM) of compounds 9 and 13 for 24 h. Quantification of gene transcripts was performed using a LightCycler ® 480 <t>TaqMan</t> according to the manufacturer’s instructions. Findings were normalized to the expression of GAPDH mRNA. Measurements were conducted in triplicate, and mean expression values for test samples relative to mean expression values for negative controls are indicated. C: control, A: arbutin (2.5 mM), C9: 3,6-dihydroxy-2,4-dimethoxy-dibenzofuran ( 9 ), C13: 3,4-dihydroxy-5-methoxybiphenyl-2′- O -β- d -glucopyranoside ( 13 ). Differences in data were evaluated for statistical significance (** p
    Lightcycler 480 Plates, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 plates/product/Roche
    Average 88 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 plates - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    89
    Thermo Fisher lightcycler 480 system
    Real-time PCR analysis of microphthalmia-associated transcription factor (MITF) and Paired box 3 (PAX3) in human epidermal melanocytes treated with 3,6-dihydroxy-2,4-dimethoxy-dibenzofuran ( 9 ) and 3,4-dihydroxy-5-methoxybiphenyl-2′- O -β- d -glucopyranoside ( 13 ). Cells (1 × 10 6 ) were treated with different concentrations (100, 80, and 60 µM) of compounds 9 and 13 for 24 h. Quantification of gene transcripts was performed using a LightCycler ® 480 <t>TaqMan</t> according to the manufacturer’s instructions. Findings were normalized to the expression of GAPDH mRNA. Measurements were conducted in triplicate, and mean expression values for test samples relative to mean expression values for negative controls are indicated. C: control, A: arbutin (2.5 mM), C9: 3,6-dihydroxy-2,4-dimethoxy-dibenzofuran ( 9 ), C13: 3,4-dihydroxy-5-methoxybiphenyl-2′- O -β- d -glucopyranoside ( 13 ). Differences in data were evaluated for statistical significance (** p
    Lightcycler 480 System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 system/product/Thermo Fisher
    Average 89 stars, based on 125 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 system - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    80
    Roche lightcycler 480 probesmaster
    UL138 transcripts are polycistronic. (A) HEK-293 cells were transfected with cDNAs representing the 3.6-, 2.7- and 1.4-kb UL138 transcripts or constructs encoding individual myc-tagged ORFs and analyzed by immunoblotting using polyclonal rabbit antisera specific to pUL133, pUL135, pUL136, and pUL138 or a monoclonal antibody specific to tubulin as a loading control. (B) The intensities of the pUL138 band were quantitated with a Li-Cor Odyssey imaging system and are represented graphically. (C) RNA isolated from lysates described in panel A was analyzed by qRT-PCR. Inverse crossing points (1/Ct) were determined using the Roche <t>LightCycler</t> 480 software with the second derivative max method (graphed). Fold change compares amplifications of the 5′ and 3′ ends of experimental cDNAs isolated from transfected cells to cDNA derived from full-length iv-RNA. Transcripts with fold changes ≤2 compared to iv-RNA were considered to be full length.
    Lightcycler 480 Probesmaster, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 probesmaster/product/Roche
    Average 80 stars, based on 207 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 probesmaster - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    88
    Roche lightcycler model 480
    UL138 transcripts are polycistronic. (A) HEK-293 cells were transfected with cDNAs representing the 3.6-, 2.7- and 1.4-kb UL138 transcripts or constructs encoding individual myc-tagged ORFs and analyzed by immunoblotting using polyclonal rabbit antisera specific to pUL133, pUL135, pUL136, and pUL138 or a monoclonal antibody specific to tubulin as a loading control. (B) The intensities of the pUL138 band were quantitated with a Li-Cor Odyssey imaging system and are represented graphically. (C) RNA isolated from lysates described in panel A was analyzed by qRT-PCR. Inverse crossing points (1/Ct) were determined using the Roche <t>LightCycler</t> 480 software with the second derivative max method (graphed). Fold change compares amplifications of the 5′ and 3′ ends of experimental cDNAs isolated from transfected cells to cDNA derived from full-length iv-RNA. Transcripts with fold changes ≤2 compared to iv-RNA were considered to be full length.
    Lightcycler Model 480, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler model 480/product/Roche
    Average 88 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    lightcycler model 480 - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    79
    PEQLAB lightcycler 480 kit
    UL138 transcripts are polycistronic. (A) HEK-293 cells were transfected with cDNAs representing the 3.6-, 2.7- and 1.4-kb UL138 transcripts or constructs encoding individual myc-tagged ORFs and analyzed by immunoblotting using polyclonal rabbit antisera specific to pUL133, pUL135, pUL136, and pUL138 or a monoclonal antibody specific to tubulin as a loading control. (B) The intensities of the pUL138 band were quantitated with a Li-Cor Odyssey imaging system and are represented graphically. (C) RNA isolated from lysates described in panel A was analyzed by qRT-PCR. Inverse crossing points (1/Ct) were determined using the Roche <t>LightCycler</t> 480 software with the second derivative max method (graphed). Fold change compares amplifications of the 5′ and 3′ ends of experimental cDNAs isolated from transfected cells to cDNA derived from full-length iv-RNA. Transcripts with fold changes ≤2 compared to iv-RNA were considered to be full length.
    Lightcycler 480 Kit, supplied by PEQLAB, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 kit/product/PEQLAB
    Average 79 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 kit - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    80
    Roche lightcycler 480 mastermix
    UL138 transcripts are polycistronic. (A) HEK-293 cells were transfected with cDNAs representing the 3.6-, 2.7- and 1.4-kb UL138 transcripts or constructs encoding individual myc-tagged ORFs and analyzed by immunoblotting using polyclonal rabbit antisera specific to pUL133, pUL135, pUL136, and pUL138 or a monoclonal antibody specific to tubulin as a loading control. (B) The intensities of the pUL138 band were quantitated with a Li-Cor Odyssey imaging system and are represented graphically. (C) RNA isolated from lysates described in panel A was analyzed by qRT-PCR. Inverse crossing points (1/Ct) were determined using the Roche <t>LightCycler</t> 480 software with the second derivative max method (graphed). Fold change compares amplifications of the 5′ and 3′ ends of experimental cDNAs isolated from transfected cells to cDNA derived from full-length iv-RNA. Transcripts with fold changes ≤2 compared to iv-RNA were considered to be full length.
    Lightcycler 480 Mastermix, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 mastermix/product/Roche
    Average 80 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 mastermix - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    79
    Roche lightcycler 480 analyzer
    UL138 transcripts are polycistronic. (A) HEK-293 cells were transfected with cDNAs representing the 3.6-, 2.7- and 1.4-kb UL138 transcripts or constructs encoding individual myc-tagged ORFs and analyzed by immunoblotting using polyclonal rabbit antisera specific to pUL133, pUL135, pUL136, and pUL138 or a monoclonal antibody specific to tubulin as a loading control. (B) The intensities of the pUL138 band were quantitated with a Li-Cor Odyssey imaging system and are represented graphically. (C) RNA isolated from lysates described in panel A was analyzed by qRT-PCR. Inverse crossing points (1/Ct) were determined using the Roche <t>LightCycler</t> 480 software with the second derivative max method (graphed). Fold change compares amplifications of the 5′ and 3′ ends of experimental cDNAs isolated from transfected cells to cDNA derived from full-length iv-RNA. Transcripts with fold changes ≤2 compared to iv-RNA were considered to be full length.
    Lightcycler 480 Analyzer, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 analyzer/product/Roche
    Average 79 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 analyzer - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    87
    Roche 384w lightcycler 480
    UL138 transcripts are polycistronic. (A) HEK-293 cells were transfected with cDNAs representing the 3.6-, 2.7- and 1.4-kb UL138 transcripts or constructs encoding individual myc-tagged ORFs and analyzed by immunoblotting using polyclonal rabbit antisera specific to pUL133, pUL135, pUL136, and pUL138 or a monoclonal antibody specific to tubulin as a loading control. (B) The intensities of the pUL138 band were quantitated with a Li-Cor Odyssey imaging system and are represented graphically. (C) RNA isolated from lysates described in panel A was analyzed by qRT-PCR. Inverse crossing points (1/Ct) were determined using the Roche <t>LightCycler</t> 480 software with the second derivative max method (graphed). Fold change compares amplifications of the 5′ and 3′ ends of experimental cDNAs isolated from transfected cells to cDNA derived from full-length iv-RNA. Transcripts with fold changes ≤2 compared to iv-RNA were considered to be full length.
    384w Lightcycler 480, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/384w lightcycler 480/product/Roche
    Average 87 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    384w lightcycler 480 - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    93
    Stratagene roche lightcycler 480
    UL138 transcripts are polycistronic. (A) HEK-293 cells were transfected with cDNAs representing the 3.6-, 2.7- and 1.4-kb UL138 transcripts or constructs encoding individual myc-tagged ORFs and analyzed by immunoblotting using polyclonal rabbit antisera specific to pUL133, pUL135, pUL136, and pUL138 or a monoclonal antibody specific to tubulin as a loading control. (B) The intensities of the pUL138 band were quantitated with a Li-Cor Odyssey imaging system and are represented graphically. (C) RNA isolated from lysates described in panel A was analyzed by qRT-PCR. Inverse crossing points (1/Ct) were determined using the Roche <t>LightCycler</t> 480 software with the second derivative max method (graphed). Fold change compares amplifications of the 5′ and 3′ ends of experimental cDNAs isolated from transfected cells to cDNA derived from full-length iv-RNA. Transcripts with fold changes ≤2 compared to iv-RNA were considered to be full length.
    Roche Lightcycler 480, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/roche lightcycler 480/product/Stratagene
    Average 93 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    roche lightcycler 480 - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    75
    Roche lightcycler 480 kit
    UL138 transcripts are polycistronic. (A) HEK-293 cells were transfected with cDNAs representing the 3.6-, 2.7- and 1.4-kb UL138 transcripts or constructs encoding individual myc-tagged ORFs and analyzed by immunoblotting using polyclonal rabbit antisera specific to pUL133, pUL135, pUL136, and pUL138 or a monoclonal antibody specific to tubulin as a loading control. (B) The intensities of the pUL138 band were quantitated with a Li-Cor Odyssey imaging system and are represented graphically. (C) RNA isolated from lysates described in panel A was analyzed by qRT-PCR. Inverse crossing points (1/Ct) were determined using the Roche <t>LightCycler</t> 480 software with the second derivative max method (graphed). Fold change compares amplifications of the 5′ and 3′ ends of experimental cDNAs isolated from transfected cells to cDNA derived from full-length iv-RNA. Transcripts with fold changes ≤2 compared to iv-RNA were considered to be full length.
    Lightcycler 480 Kit, supplied by Roche, used in various techniques. Bioz Stars score: 75/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 kit/product/Roche
    Average 75 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 kit - by Bioz Stars, 2020-02
    75/100 stars
      Buy from Supplier

    79
    Roche lightcycler 480 sybgreen
    UL138 transcripts are polycistronic. (A) HEK-293 cells were transfected with cDNAs representing the 3.6-, 2.7- and 1.4-kb UL138 transcripts or constructs encoding individual myc-tagged ORFs and analyzed by immunoblotting using polyclonal rabbit antisera specific to pUL133, pUL135, pUL136, and pUL138 or a monoclonal antibody specific to tubulin as a loading control. (B) The intensities of the pUL138 band were quantitated with a Li-Cor Odyssey imaging system and are represented graphically. (C) RNA isolated from lysates described in panel A was analyzed by qRT-PCR. Inverse crossing points (1/Ct) were determined using the Roche <t>LightCycler</t> 480 software with the second derivative max method (graphed). Fold change compares amplifications of the 5′ and 3′ ends of experimental cDNAs isolated from transfected cells to cDNA derived from full-length iv-RNA. Transcripts with fold changes ≤2 compared to iv-RNA were considered to be full length.
    Lightcycler 480 Sybgreen, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 sybgreen/product/Roche
    Average 79 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 sybgreen - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    87
    Roche lightcycler 480 real time lightcycler
    UL138 transcripts are polycistronic. (A) HEK-293 cells were transfected with cDNAs representing the 3.6-, 2.7- and 1.4-kb UL138 transcripts or constructs encoding individual myc-tagged ORFs and analyzed by immunoblotting using polyclonal rabbit antisera specific to pUL133, pUL135, pUL136, and pUL138 or a monoclonal antibody specific to tubulin as a loading control. (B) The intensities of the pUL138 band were quantitated with a Li-Cor Odyssey imaging system and are represented graphically. (C) RNA isolated from lysates described in panel A was analyzed by qRT-PCR. Inverse crossing points (1/Ct) were determined using the Roche <t>LightCycler</t> 480 software with the second derivative max method (graphed). Fold change compares amplifications of the 5′ and 3′ ends of experimental cDNAs isolated from transfected cells to cDNA derived from full-length iv-RNA. Transcripts with fold changes ≤2 compared to iv-RNA were considered to be full length.
    Lightcycler 480 Real Time Lightcycler, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lightcycler 480 real time lightcycler/product/Roche
    Average 87 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    lightcycler 480 real time lightcycler - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    Image Search Results


    Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for  M. tuberculosis , 47.5°C to 50.5°C for  M. avium  and 57.5°C to 60.5°C for  M. kansasii.

    Journal: BMC Infectious Diseases

    Article Title: Use of real time polymerase chain reaction for detection of M. tuberculosis, M. avium and M. kansasii from clinical specimens

    doi: 10.1186/s12879-015-0921-0

    Figure Lengend Snippet: Melting temperature determination using Tm calling on LightCycler 480® software determining the different mycobacteria species using temperature ranges; 54.4°C to 57.4°C for M. tuberculosis , 47.5°C to 50.5°C for M. avium and 57.5°C to 60.5°C for M. kansasii.

    Article Snippet: Mycobacterium real time PCR Real time PCR was performed on a LightCycler® 480 II (Roche diagnostics, Mannheim, Germany) according to the manufacturer’s instructions using light cycler mycobacterium detection assay.

    Techniques: Software

    Sigmoid amplification curves captured during anealing and elongation step by the Roche LightCycler 480 II indicating the detection of M. tuberculosis , M. avium or M. kansasii.

    Journal: BMC Infectious Diseases

    Article Title: Use of real time polymerase chain reaction for detection of M. tuberculosis, M. avium and M. kansasii from clinical specimens

    doi: 10.1186/s12879-015-0921-0

    Figure Lengend Snippet: Sigmoid amplification curves captured during anealing and elongation step by the Roche LightCycler 480 II indicating the detection of M. tuberculosis , M. avium or M. kansasii.

    Article Snippet: Mycobacterium real time PCR Real time PCR was performed on a LightCycler® 480 II (Roche diagnostics, Mannheim, Germany) according to the manufacturer’s instructions using light cycler mycobacterium detection assay.

    Techniques: Amplification

    Negative effect of the use of the SuperScript III Platinum One-Step quantitative RT-PCR kit on the specificity of the MeVA RT-qPCR for the vaccine genotype. (A) The use of SuperScript III on the Roche LightCycler 480 platform caused a significant rise

    Journal: Journal of Clinical Microbiology

    Article Title: Rapid Identification of Measles Virus Vaccine Genotype by Real-Time PCR

    doi: 10.1128/JCM.01879-16

    Figure Lengend Snippet: Negative effect of the use of the SuperScript III Platinum One-Step quantitative RT-PCR kit on the specificity of the MeVA RT-qPCR for the vaccine genotype. (A) The use of SuperScript III on the Roche LightCycler 480 platform caused a significant rise

    Article Snippet: The results were similar to those obtained from the Roche LightCycler 480 system ( ) in that the lower limit of detection of the MeVA RT-qPCR assay was approximately 1 Log10 higher than for the MeV RT-qPCR assay.

    Techniques: Quantitative RT-PCR

    Amplification curve from the MeVA RT-qPCR on the Roche LightCycler 480 system. The bundle of the flat curves includes 33 wild-type measles virus specimens comprising the following genotypes: B2, B3, C1, C2, D2, D3, D4, D5, D6, D7, D8, D9, D10, G1, G2,

    Journal: Journal of Clinical Microbiology

    Article Title: Rapid Identification of Measles Virus Vaccine Genotype by Real-Time PCR

    doi: 10.1128/JCM.01879-16

    Figure Lengend Snippet: Amplification curve from the MeVA RT-qPCR on the Roche LightCycler 480 system. The bundle of the flat curves includes 33 wild-type measles virus specimens comprising the following genotypes: B2, B3, C1, C2, D2, D3, D4, D5, D6, D7, D8, D9, D10, G1, G2,

    Article Snippet: The results were similar to those obtained from the Roche LightCycler 480 system ( ) in that the lower limit of detection of the MeVA RT-qPCR assay was approximately 1 Log10 higher than for the MeV RT-qPCR assay.

    Techniques: Amplification, Quantitative RT-PCR

    Assembly curves produced from the Roche 480 Lightcycler using SYBR green II reporter dye. (A) 3WJ RNA , (B) 3WJ DNA , (C) 3WJ 2′-F , and each of the components of the 3WJs. (D) The three 3WJ species directly compared, showing the differences in annealing temperatures ( T a ).

    Journal: Biochemistry

    Article Title: Entropy-Driven One-Step Formation of Phi29 pRNA 3WJ from Three RNA Fragments

    doi: 10.1021/bi4017022

    Figure Lengend Snippet: Assembly curves produced from the Roche 480 Lightcycler using SYBR green II reporter dye. (A) 3WJ RNA , (B) 3WJ DNA , (C) 3WJ 2′-F , and each of the components of the 3WJs. (D) The three 3WJ species directly compared, showing the differences in annealing temperatures ( T a ).

    Article Snippet: Determination of the Formation of Complex and T a of DNA, RNA, and 2′-F RNA-3WJs by rtPCR Machine The thermostability of 3WJs complexes were compared by measuring their fluorescence intensities as a function of temperature in the presence of SYBR Green II dye on a Roche 480 Lightcycler to obtain T a .

    Techniques: Produced, SYBR Green Assay

    Comparison of pRNA-3WJ hybrid structures. Assembly curves produced from the Roche 480 Lightcycler of the pRNA-3WJ (A) RNA/DNA hybrids, (B) RNA/2′-F RNA hybrids, and (C) DNA/2′-F RNA hyrbids.

    Journal: Biochemistry

    Article Title: Entropy-Driven One-Step Formation of Phi29 pRNA 3WJ from Three RNA Fragments

    doi: 10.1021/bi4017022

    Figure Lengend Snippet: Comparison of pRNA-3WJ hybrid structures. Assembly curves produced from the Roche 480 Lightcycler of the pRNA-3WJ (A) RNA/DNA hybrids, (B) RNA/2′-F RNA hybrids, and (C) DNA/2′-F RNA hyrbids.

    Article Snippet: Determination of the Formation of Complex and T a of DNA, RNA, and 2′-F RNA-3WJs by rtPCR Machine The thermostability of 3WJs complexes were compared by measuring their fluorescence intensities as a function of temperature in the presence of SYBR Green II dye on a Roche 480 Lightcycler to obtain T a .

    Techniques: Produced

    Real-time PCR analysis of microphthalmia-associated transcription factor (MITF) and Paired box 3 (PAX3) in human epidermal melanocytes treated with 3,6-dihydroxy-2,4-dimethoxy-dibenzofuran ( 9 ) and 3,4-dihydroxy-5-methoxybiphenyl-2′- O -β- d -glucopyranoside ( 13 ). Cells (1 × 10 6 ) were treated with different concentrations (100, 80, and 60 µM) of compounds 9 and 13 for 24 h. Quantification of gene transcripts was performed using a LightCycler ® 480 TaqMan according to the manufacturer’s instructions. Findings were normalized to the expression of GAPDH mRNA. Measurements were conducted in triplicate, and mean expression values for test samples relative to mean expression values for negative controls are indicated. C: control, A: arbutin (2.5 mM), C9: 3,6-dihydroxy-2,4-dimethoxy-dibenzofuran ( 9 ), C13: 3,4-dihydroxy-5-methoxybiphenyl-2′- O -β- d -glucopyranoside ( 13 ). Differences in data were evaluated for statistical significance (** p

    Journal: International Journal of Molecular Sciences

    Article Title: New Whitening Constituents from Taiwan-Native Pyracantha koidzumii: Structures and Tyrosinase Inhibitory Analysis in Human Epidermal Melanocytes

    doi: 10.3390/ijms161226115

    Figure Lengend Snippet: Real-time PCR analysis of microphthalmia-associated transcription factor (MITF) and Paired box 3 (PAX3) in human epidermal melanocytes treated with 3,6-dihydroxy-2,4-dimethoxy-dibenzofuran ( 9 ) and 3,4-dihydroxy-5-methoxybiphenyl-2′- O -β- d -glucopyranoside ( 13 ). Cells (1 × 10 6 ) were treated with different concentrations (100, 80, and 60 µM) of compounds 9 and 13 for 24 h. Quantification of gene transcripts was performed using a LightCycler ® 480 TaqMan according to the manufacturer’s instructions. Findings were normalized to the expression of GAPDH mRNA. Measurements were conducted in triplicate, and mean expression values for test samples relative to mean expression values for negative controls are indicated. C: control, A: arbutin (2.5 mM), C9: 3,6-dihydroxy-2,4-dimethoxy-dibenzofuran ( 9 ), C13: 3,4-dihydroxy-5-methoxybiphenyl-2′- O -β- d -glucopyranoside ( 13 ). Differences in data were evaluated for statistical significance (** p

    Article Snippet: Real-Time PCR Analysis Quantification of genes transcript by real-time PCR was performed using a LightCycler® 480 TaqMan (Roche, Mannheim, Germany) according to the manufacturer’s instructions.

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    UL138 transcripts are polycistronic. (A) HEK-293 cells were transfected with cDNAs representing the 3.6-, 2.7- and 1.4-kb UL138 transcripts or constructs encoding individual myc-tagged ORFs and analyzed by immunoblotting using polyclonal rabbit antisera specific to pUL133, pUL135, pUL136, and pUL138 or a monoclonal antibody specific to tubulin as a loading control. (B) The intensities of the pUL138 band were quantitated with a Li-Cor Odyssey imaging system and are represented graphically. (C) RNA isolated from lysates described in panel A was analyzed by qRT-PCR. Inverse crossing points (1/Ct) were determined using the Roche LightCycler 480 software with the second derivative max method (graphed). Fold change compares amplifications of the 5′ and 3′ ends of experimental cDNAs isolated from transfected cells to cDNA derived from full-length iv-RNA. Transcripts with fold changes ≤2 compared to iv-RNA were considered to be full length.

    Journal: Journal of Virology

    Article Title: Stress-Inducible Alternative Translation Initiation of Human Cytomegalovirus Latency Protein pUL138 ▿

    doi: 10.1128/JVI.00855-10

    Figure Lengend Snippet: UL138 transcripts are polycistronic. (A) HEK-293 cells were transfected with cDNAs representing the 3.6-, 2.7- and 1.4-kb UL138 transcripts or constructs encoding individual myc-tagged ORFs and analyzed by immunoblotting using polyclonal rabbit antisera specific to pUL133, pUL135, pUL136, and pUL138 or a monoclonal antibody specific to tubulin as a loading control. (B) The intensities of the pUL138 band were quantitated with a Li-Cor Odyssey imaging system and are represented graphically. (C) RNA isolated from lysates described in panel A was analyzed by qRT-PCR. Inverse crossing points (1/Ct) were determined using the Roche LightCycler 480 software with the second derivative max method (graphed). Fold change compares amplifications of the 5′ and 3′ ends of experimental cDNAs isolated from transfected cells to cDNA derived from full-length iv-RNA. Transcripts with fold changes ≤2 compared to iv-RNA were considered to be full length.

    Article Snippet: Quantitative reverse transcription PCR (qRT-PCR) was performed with the LightCycler 480 Probes Master (Roche) according to the manufacturer's instructions along with Universal Probe Library (Roche) probes and primers specific for UL133 (forward, 5′-TTTCTGGACCTGGCATCG-3′; reverse, 5′-TACGCGCAGAGGAAAATCAT-3′; probe number 114, 5′-CTGTGGTC-3′), UL135 (forward, 5′-GCGGTGTACGTCGCTCTAC-3′; reverse, 5′-GGAAACTCGGGTTTATCTATCG-3′; probe number 55, 5′-GGCAGACCATCCTCTCCTATG-3′), UL136 (forward, 5′-GCGGCTGTCATTATCCTGAG-3′; reverse, 5′-TAGTACATGGCCCCGTTCA-3′; probe number 152, 5′-TCGCCGTC-3′), UL138 (forward, 5′-GGTTCATCGTCTTCGTCGTC-3′; reverse, 5′-CACGGGTTTCAACAGATCG-3′; probe number 126, 5′-TCTCTGGT-3′), and firefly luciferase (FL) (forward, 5′-TGAGTACTTCGAAATGTCCGTTC-3′; reverse, 5′-GTATTCAGCCCATATCGTTTCAT-3′; probe number 29, 5′-GAAGACGG-3′) and the prevalidated UPL human beta-actin gene assay (Roche).

    Techniques: Transfection, Construct, Imaging, Isolation, Quantitative RT-PCR, Software, Derivative Assay

    Principle of high-resolution amplicon melting-curve analysis to produce fluorescence difference plot curves. Melting-curve acquisitions and analyses were performed on the LightCycler 480 instrument (Roche). PCR products were denatured at 95°C for 60 s, renatured at 40°C for 60 s, and then melted at 60°C to 97°C with 25 signal acquisitions per degree. Melting-curve analysis was then performed using the LightCycler 480 gene-scanning software module. This software analyzes the HRM curve data to identify changes in the shape of the curve (difference plot curve) that indicate sequence polymorphisms. The amplicon melting curve is automatically calculated by the software in a three-step process, shown in the three panels. (A) Normalization. The raw data (left) are normalized to both 100% (at the beginning of the melting) and 0% (at the end of the melting). (Left) Raw data; (right) the same curve after normalization. (B) Temperature shift. (Left) The point (ellipse) at which the entire double-stranded DNA was completely denatured; (right) the same curve after the temperature has been shifted along the x axis. The temperature shifting with the curve allows comparisons between samples. (C) Fluorescence difference plot. (Left) Three points that show the difference between the base curve and the samples; (right) fluorescence difference plot curve obtained by subtracting each curve from the base curve. Because of the shift in the temperature of the curve, the temperature axis no longer reflects absolute temperature but rather reflects temperature differences relative to the superimposed segment of the curves. Data for three samples (a, b, and c) are shown.

    Journal: Journal of Clinical Microbiology

    Article Title: Development of a Rapid High-Throughput Method for High-Resolution Melting Analysis for Routine Detection and Genotyping of Noroviruses ▿

    doi: 10.1128/JCM.01247-08

    Figure Lengend Snippet: Principle of high-resolution amplicon melting-curve analysis to produce fluorescence difference plot curves. Melting-curve acquisitions and analyses were performed on the LightCycler 480 instrument (Roche). PCR products were denatured at 95°C for 60 s, renatured at 40°C for 60 s, and then melted at 60°C to 97°C with 25 signal acquisitions per degree. Melting-curve analysis was then performed using the LightCycler 480 gene-scanning software module. This software analyzes the HRM curve data to identify changes in the shape of the curve (difference plot curve) that indicate sequence polymorphisms. The amplicon melting curve is automatically calculated by the software in a three-step process, shown in the three panels. (A) Normalization. The raw data (left) are normalized to both 100% (at the beginning of the melting) and 0% (at the end of the melting). (Left) Raw data; (right) the same curve after normalization. (B) Temperature shift. (Left) The point (ellipse) at which the entire double-stranded DNA was completely denatured; (right) the same curve after the temperature has been shifted along the x axis. The temperature shifting with the curve allows comparisons between samples. (C) Fluorescence difference plot. (Left) Three points that show the difference between the base curve and the samples; (right) fluorescence difference plot curve obtained by subtracting each curve from the base curve. Because of the shift in the temperature of the curve, the temperature axis no longer reflects absolute temperature but rather reflects temperature differences relative to the superimposed segment of the curves. Data for three samples (a, b, and c) are shown.

    Article Snippet: PCR was performed with 2 μl of cDNA in a 20-μl reaction mixture containing 500 nM each sense and antisense primer, 200 nM TaqMan probes, and LightCycler 480 Probe Master (Roche).

    Techniques: Amplification, Fluorescence, Polymerase Chain Reaction, Software, Sequencing