lightcycler 2.0 system Search Results


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  • 99
    Roche lightcycler 2 0 system
    Alteration of the VCP/p97 expression during differentiation and retrodifferentiation .  A . U937 cells were treated initially with 10 nM TPA and then cultured in the absence of TPA until retrodifferentiation. At the time points indicated, cell lysates were separated by 10% SDS-PAGE followed by Western blot analysis with anti-VCP/p97, anti-P-tyr, anti-p21 Cip1/Waf1/sdi-1  and anti-vimentin antibody. The tyrosine phosphorylation pattern (p-tyr) is demonstrated by the complete range from 250-20 kDa. Staining with β-actin was used as a loading control. Densitometric analysis and normalization to β-actin was performed using the ImageJ software (NIH, Bethesda, MD, USA).  B . Quantitative RT-PCR analysis of VCP/p97 mRNA levels during differentiation and retrodifferentiation. Following total RNA isolation of undifferentiated U937 control cells (U937), differentiated populations (3d until 18d) and retrodifferentiated U937 cells (Retro), the amplification was performed in the LightCycler 2.0 System using the LightCycler Software 3.5. Results represent means ± SD from three independent experiments.
    Lightcycler 2 0 System, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1035 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    lightcycler 2 0 system - by Bioz Stars, 2020-03
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    79
    Roche portable lightcycler 2 0 system
    Alteration of the VCP/p97 expression during differentiation and retrodifferentiation .  A . U937 cells were treated initially with 10 nM TPA and then cultured in the absence of TPA until retrodifferentiation. At the time points indicated, cell lysates were separated by 10% SDS-PAGE followed by Western blot analysis with anti-VCP/p97, anti-P-tyr, anti-p21 Cip1/Waf1/sdi-1  and anti-vimentin antibody. The tyrosine phosphorylation pattern (p-tyr) is demonstrated by the complete range from 250-20 kDa. Staining with β-actin was used as a loading control. Densitometric analysis and normalization to β-actin was performed using the ImageJ software (NIH, Bethesda, MD, USA).  B . Quantitative RT-PCR analysis of VCP/p97 mRNA levels during differentiation and retrodifferentiation. Following total RNA isolation of undifferentiated U937 control cells (U937), differentiated populations (3d until 18d) and retrodifferentiated U937 cells (Retro), the amplification was performed in the LightCycler 2.0 System using the LightCycler Software 3.5. Results represent means ± SD from three independent experiments.
    Portable Lightcycler 2 0 System, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Roche lightcycler 2 0 system software package
    Alteration of the VCP/p97 expression during differentiation and retrodifferentiation .  A . U937 cells were treated initially with 10 nM TPA and then cultured in the absence of TPA until retrodifferentiation. At the time points indicated, cell lysates were separated by 10% SDS-PAGE followed by Western blot analysis with anti-VCP/p97, anti-P-tyr, anti-p21 Cip1/Waf1/sdi-1  and anti-vimentin antibody. The tyrosine phosphorylation pattern (p-tyr) is demonstrated by the complete range from 250-20 kDa. Staining with β-actin was used as a loading control. Densitometric analysis and normalization to β-actin was performed using the ImageJ software (NIH, Bethesda, MD, USA).  B . Quantitative RT-PCR analysis of VCP/p97 mRNA levels during differentiation and retrodifferentiation. Following total RNA isolation of undifferentiated U937 control cells (U937), differentiated populations (3d until 18d) and retrodifferentiated U937 cells (Retro), the amplification was performed in the LightCycler 2.0 System using the LightCycler Software 3.5. Results represent means ± SD from three independent experiments.
    Lightcycler 2 0 System Software Package, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Roche capillary roche lightcycler 2 0 system
    Alteration of the VCP/p97 expression during differentiation and retrodifferentiation .  A . U937 cells were treated initially with 10 nM TPA and then cultured in the absence of TPA until retrodifferentiation. At the time points indicated, cell lysates were separated by 10% SDS-PAGE followed by Western blot analysis with anti-VCP/p97, anti-P-tyr, anti-p21 Cip1/Waf1/sdi-1  and anti-vimentin antibody. The tyrosine phosphorylation pattern (p-tyr) is demonstrated by the complete range from 250-20 kDa. Staining with β-actin was used as a loading control. Densitometric analysis and normalization to β-actin was performed using the ImageJ software (NIH, Bethesda, MD, USA).  B . Quantitative RT-PCR analysis of VCP/p97 mRNA levels during differentiation and retrodifferentiation. Following total RNA isolation of undifferentiated U937 control cells (U937), differentiated populations (3d until 18d) and retrodifferentiated U937 cells (Retro), the amplification was performed in the LightCycler 2.0 System using the LightCycler Software 3.5. Results represent means ± SD from three independent experiments.
    Capillary Roche Lightcycler 2 0 System, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Roche applied science lightcycler 2 0 system
    Alteration of the VCP/p97 expression during differentiation and retrodifferentiation .  A . U937 cells were treated initially with 10 nM TPA and then cultured in the absence of TPA until retrodifferentiation. At the time points indicated, cell lysates were separated by 10% SDS-PAGE followed by Western blot analysis with anti-VCP/p97, anti-P-tyr, anti-p21 Cip1/Waf1/sdi-1  and anti-vimentin antibody. The tyrosine phosphorylation pattern (p-tyr) is demonstrated by the complete range from 250-20 kDa. Staining with β-actin was used as a loading control. Densitometric analysis and normalization to β-actin was performed using the ImageJ software (NIH, Bethesda, MD, USA).  B . Quantitative RT-PCR analysis of VCP/p97 mRNA levels during differentiation and retrodifferentiation. Following total RNA isolation of undifferentiated U937 control cells (U937), differentiated populations (3d until 18d) and retrodifferentiated U937 cells (Retro), the amplification was performed in the LightCycler 2.0 System using the LightCycler Software 3.5. Results represent means ± SD from three independent experiments.
    Applied Science Lightcycler 2 0 System, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Roche lightcycler 2 0
    Bland-Altman representation of CMV DNA loads (copies/ml) measured using the Abbott CMV PCR kit in the m2000RT (ABBOTT), the LightCycler CMV Quant kit in the Light Cycler 2.0 instrument (ROCHE), and the Q-CMV complete kit in the LightCycler 2.0 instrument
    Lightcycler 2 0, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 2659 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Toyobo lightcycler 2 0
    Bland-Altman representation of CMV DNA loads (copies/ml) measured using the Abbott CMV PCR kit in the m2000RT (ABBOTT), the LightCycler CMV Quant kit in the Light Cycler 2.0 instrument (ROCHE), and the Q-CMV complete kit in the LightCycler 2.0 instrument
    Lightcycler 2 0, supplied by Toyobo, used in various techniques. Bioz Stars score: 98/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Roche 480 lightcycler 2 0
    Bland-Altman representation of CMV DNA loads (copies/ml) measured using the Abbott CMV PCR kit in the m2000RT (ABBOTT), the LightCycler CMV Quant kit in the Light Cycler 2.0 instrument (ROCHE), and the Q-CMV complete kit in the LightCycler 2.0 instrument
    480 Lightcycler 2 0, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Roche lightcycler 2 0 platform
    Bland-Altman representation of CMV DNA loads (copies/ml) measured using the Abbott CMV PCR kit in the m2000RT (ABBOTT), the LightCycler CMV Quant kit in the Light Cycler 2.0 instrument (ROCHE), and the Q-CMV complete kit in the LightCycler 2.0 instrument
    Lightcycler 2 0 Platform, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Roche lightcycler 2 0 thermocycler
    Bland-Altman representation of CMV DNA loads (copies/ml) measured using the Abbott CMV PCR kit in the m2000RT (ABBOTT), the LightCycler CMV Quant kit in the Light Cycler 2.0 instrument (ROCHE), and the Q-CMV complete kit in the LightCycler 2.0 instrument
    Lightcycler 2 0 Thermocycler, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Roche lightcycler 2 0 apparatus
    Bland-Altman representation of CMV DNA loads (copies/ml) measured using the Abbott CMV PCR kit in the m2000RT (ABBOTT), the LightCycler CMV Quant kit in the Light Cycler 2.0 instrument (ROCHE), and the Q-CMV complete kit in the LightCycler 2.0 instrument
    Lightcycler 2 0 Apparatus, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Roche lightcycler 2 0 analyzer
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Analyzer, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Roche lightcycler 2 0 software
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Software, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Roche lightcycler 2 0 instrument protocol
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Instrument Protocol, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Roche lightcycler 2 0 septifast kit
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Septifast Kit, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Roche lightcycler 2 0 methods
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Methods, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Roche roche lightcycler 2 0 machine
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Roche Lightcycler 2 0 Machine, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher lightcycler 2 0 software
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Roche lightcycler 2 0 thermal cycler
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Thermal Cycler, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Roche p3 lightcycler 2 0 instruments
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    P3 Lightcycler 2 0 Instruments, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Bio-Rad lightcycler 2 0
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Roche lightcycler 2 0 pcr instrument
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Pcr Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Roche lightcycler 2 0 pcr system
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Roche lightcycler 2 0 instrument capillary
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Instrument Capillary, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Roche lightcycler 2 0 pcr machine
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Pcr Machine, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Roche lightcycler 2 0 qpcr system
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Qpcr System, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Roche lightcycler 2 0 real timepcr system
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Real Timepcr System, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Roche laboratory based lightcycler 2 0 instrument
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Laboratory Based Lightcycler 2 0 Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Roche lightcycler 2 0 real time system
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Real Time System, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Roche real time pcr lightcycler 2 0
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Real Time Pcr Lightcycler 2 0, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Roche lightcycler 2 0 ce ivd lc instrument
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Ce Ivd Lc Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Roche lightcycler 2 0 real time instrument
    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.
    Lightcycler 2 0 Real Time Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alteration of the VCP/p97 expression during differentiation and retrodifferentiation .  A . U937 cells were treated initially with 10 nM TPA and then cultured in the absence of TPA until retrodifferentiation. At the time points indicated, cell lysates were separated by 10% SDS-PAGE followed by Western blot analysis with anti-VCP/p97, anti-P-tyr, anti-p21 Cip1/Waf1/sdi-1  and anti-vimentin antibody. The tyrosine phosphorylation pattern (p-tyr) is demonstrated by the complete range from 250-20 kDa. Staining with β-actin was used as a loading control. Densitometric analysis and normalization to β-actin was performed using the ImageJ software (NIH, Bethesda, MD, USA).  B . Quantitative RT-PCR analysis of VCP/p97 mRNA levels during differentiation and retrodifferentiation. Following total RNA isolation of undifferentiated U937 control cells (U937), differentiated populations (3d until 18d) and retrodifferentiated U937 cells (Retro), the amplification was performed in the LightCycler 2.0 System using the LightCycler Software 3.5. Results represent means ± SD from three independent experiments.

    Journal: BMC Cell Biology

    Article Title: The differentiation/retrodifferentiation program of human U937 leukemia cells is accompanied by changes of VCP/p97

    doi: 10.1186/1471-2121-9-12

    Figure Lengend Snippet: Alteration of the VCP/p97 expression during differentiation and retrodifferentiation . A . U937 cells were treated initially with 10 nM TPA and then cultured in the absence of TPA until retrodifferentiation. At the time points indicated, cell lysates were separated by 10% SDS-PAGE followed by Western blot analysis with anti-VCP/p97, anti-P-tyr, anti-p21 Cip1/Waf1/sdi-1 and anti-vimentin antibody. The tyrosine phosphorylation pattern (p-tyr) is demonstrated by the complete range from 250-20 kDa. Staining with β-actin was used as a loading control. Densitometric analysis and normalization to β-actin was performed using the ImageJ software (NIH, Bethesda, MD, USA). B . Quantitative RT-PCR analysis of VCP/p97 mRNA levels during differentiation and retrodifferentiation. Following total RNA isolation of undifferentiated U937 control cells (U937), differentiated populations (3d until 18d) and retrodifferentiated U937 cells (Retro), the amplification was performed in the LightCycler 2.0 System using the LightCycler Software 3.5. Results represent means ± SD from three independent experiments.

    Article Snippet: An aliquot of about 100 ng cDNA was amplified by quantitative RT-PCR in the LightCycler 2.0 System using the Light Cycler Software 3.5 (Roche Applied Science, Mannheim, Germany) according to the LightCycler FastStart DNA MasterPLUS Hybridisation Probes protocol (Roche).

    Techniques: Expressing, Cell Culture, SDS Page, Western Blot, Staining, Software, Quantitative RT-PCR, Isolation, Amplification

    Bland-Altman representation of CMV DNA loads (copies/ml) measured using the Abbott CMV PCR kit in the m2000RT (ABBOTT), the LightCycler CMV Quant kit in the Light Cycler 2.0 instrument (ROCHE), and the Q-CMV complete kit in the LightCycler 2.0 instrument

    Journal: Journal of Clinical Microbiology

    Article Title: Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients ▿

    doi: 10.1128/JCM.00785-11

    Figure Lengend Snippet: Bland-Altman representation of CMV DNA loads (copies/ml) measured using the Abbott CMV PCR kit in the m2000RT (ABBOTT), the LightCycler CMV Quant kit in the Light Cycler 2.0 instrument (ROCHE), and the Q-CMV complete kit in the LightCycler 2.0 instrument

    Article Snippet: The LightCycler CMV Quant kit (Roche Diagnostics GmbH, Mannheim, Germany), which targets the UL54 DNA polymerase gene of CMV, was evaluated using the LightCycler 2.0 instrument (Roche).

    Techniques: Polymerase Chain Reaction

    Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.

    Journal: World Journal of Gastroenterology

    Article Title: New real-time-PCR method to identify single point mutations in hepatitis C virus

    doi: 10.3748/wjg.v22.i43.9604

    Figure Lengend Snippet: Melting peaks of the Q and K variants analyzed by LightCycler 2.0. The variants were easily identified by their peak patterns, resulting from their characteristic melting temperatures. A: Tm for variant K approximate 59 °C; B: Tm for variant Q approximate 53 °C.

    Article Snippet: Target region, primers, and probes for real-time PCR Real-time PCR was performed in the LightCycler 2.0 analyzer (Roche Diagnostic, Mannheim, Germany).

    Techniques: Variant Assay