Journal: BMC Infectious Diseases
Article Title: Validation of a simplex PCR assay enabling reliable identification of clinically relevant Candida species
Figure Lengend Snippet: Discriminatory property of the CanTub-simplex PCR. Species level identification was achieved by using CanTub-simplex PCR primer pair to accurately identify Candida species based on HRM analysis (P1) by using the LightCycler®96 HRM analysis Software (Roche Diagnostics, Risch-Rotkreuz, Switzerland). a Representation of the normalized CanTub-simplex PCR melting curves. Each colour represents a different species: Candida albicans (blue) ID1–7; Candida glabrata (red) ID8–11; Candida parapsilosis (yellow) ID12–17; Candida tropicalis (orange) ID18–24; Candida krusei (green) ID25–30; Candida guilliermondii (brown) ID31–34; Candida dubliniensis (pink) ID35–38. b Representation of the normalized difference plots. Normalized difference plots against Candida albicans
Article Snippet: Temperature gradient assay was performed from 55 to 72 °C for assessing the performance of the primer pair during amplification with a temperature gradient program using the LightCycler® 96 thermal cycler Instrument (Roche Applied Science, Penzberg, Germany).
Techniques: Polymerase Chain Reaction, Software