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  • 99
    Thermo Fisher sybr select master mix
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    Qiagen quantifast sybr green pcr kit
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    Thermo Fisher gene exp slc22a8 mm00459534 m1
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
    Gene Exp Slc22a8 Mm00459534 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher first strand cdna synthesis
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
    First Strand Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29583 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad iscript cdna kit
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
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    Thermo Fisher powerup sybr green master mix
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
    Powerup Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa primescript rt reagent kit
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
    Primescript Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 72258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
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    Thermo Fisher taqman genotyping master mix
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
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    Thermo Fisher trizol reagent
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
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    Thermo Fisher 7900ht fast real time pcr
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
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    Thermo Fisher total rna
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
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    Thermo Fisher sybr green i nucleic acid gel stain
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
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    Toyobo kod sybr qpcr mix kit
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
    Kod Sybr Qpcr Mix Kit, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher capacity cdna reverse transcription kit
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
    Capacity Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2993 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioTek Instruments elisa
    Gene silencing by intravenous administration of <t>OAT3</t> -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P
    Elisa, supplied by BioTek Instruments, used in various techniques. Bioz Stars score: 93/100, based on 454 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Gene silencing by intravenous administration of OAT3 -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P

    Journal: Scientific Reports

    Article Title: Modulation of blood-brain barrier function by a heteroduplex oligonucleotide in vivo

    doi: 10.1038/s41598-018-22577-2

    Figure Lengend Snippet: Gene silencing by intravenous administration of OAT3 -targeting oligonucleotides. OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1, 18, or 30) at doses corresponding to 16 mg/kg of ASO; (B) 13-, 14-, or 16-mer Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (C) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to Claudin-5 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P

    Article Snippet: The primers and probes for mouse OAT3 (Mm00459534_m1), Claudin-5 (Mm00727012_s1), CD31 ( Mm01242584_m1) , U6 (001973), Interferon-β (IFN-β ; Mm00439546_s1), Interferon-γ (IFN-γ ; Mm00801778_m1), Tumor necrosis factor-α (TNF-α ; Mm00443258_m1), Interleukin-6 (IL-6 ; Mm00446190_m1), and Glyceraldehyde-3-phosphate dehydrogenase (Gapdh ; 4352932E), and each ASO strand were designed by Thermo Fisher Scientific.

    Techniques: Quantitative RT-PCR, Injection, Allele-specific Oligonucleotide

    Quantification of the parent ASO strand after intravenous administration of OAT3 -targeting oligonucleotides. Amounts of the parent ASO strand in the brain measured by quantitative RT-PCR 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (B) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to mouse U6 RNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P

    Journal: Scientific Reports

    Article Title: Modulation of blood-brain barrier function by a heteroduplex oligonucleotide in vivo

    doi: 10.1038/s41598-018-22577-2

    Figure Lengend Snippet: Quantification of the parent ASO strand after intravenous administration of OAT3 -targeting oligonucleotides. Amounts of the parent ASO strand in the brain measured by quantitative RT-PCR 72 h after an intravenous injection of (A) 13-mer ASO, HDO, or Toc-HDO (No. 1 or 18) at doses corresponding to 16 mg/kg of ASO; (B) 13-mer Toc-HDO (No. 1 or 18) at different ASO doses. Data shown are relative to mouse U6 RNA levels and are expressed as mean values ± s.e.m. ( N = 3, * P

    Article Snippet: The primers and probes for mouse OAT3 (Mm00459534_m1), Claudin-5 (Mm00727012_s1), CD31 ( Mm01242584_m1) , U6 (001973), Interferon-β (IFN-β ; Mm00439546_s1), Interferon-γ (IFN-γ ; Mm00801778_m1), Tumor necrosis factor-α (TNF-α ; Mm00443258_m1), Interleukin-6 (IL-6 ; Mm00446190_m1), and Glyceraldehyde-3-phosphate dehydrogenase (Gapdh ; 4352932E), and each ASO strand were designed by Thermo Fisher Scientific.

    Techniques: Allele-specific Oligonucleotide, Quantitative RT-PCR, Injection

    Retention of an OAT3 substrate in the brain after intravenous administration of Toc-HDO. ( A ) Schematic representation of the transport of 99m Tc-ECD and its metabolite in the brain. (B ) Radioactivity of the 99m Tc-ECD metabolite in the brain measured 2 or 30 min after an intravenous injection of 99m Tc-ECD into mice that were injected 72 h earlier with PBS (open squares) or 13-mer Toc-HDO (No. 18) at a dose corresponding to 50 mg/kg of ASO (closed squares). Data are expressed as mean values ± s.e.m. ( N = 5, * P

    Journal: Scientific Reports

    Article Title: Modulation of blood-brain barrier function by a heteroduplex oligonucleotide in vivo

    doi: 10.1038/s41598-018-22577-2

    Figure Lengend Snippet: Retention of an OAT3 substrate in the brain after intravenous administration of Toc-HDO. ( A ) Schematic representation of the transport of 99m Tc-ECD and its metabolite in the brain. (B ) Radioactivity of the 99m Tc-ECD metabolite in the brain measured 2 or 30 min after an intravenous injection of 99m Tc-ECD into mice that were injected 72 h earlier with PBS (open squares) or 13-mer Toc-HDO (No. 18) at a dose corresponding to 50 mg/kg of ASO (closed squares). Data are expressed as mean values ± s.e.m. ( N = 5, * P

    Article Snippet: The primers and probes for mouse OAT3 (Mm00459534_m1), Claudin-5 (Mm00727012_s1), CD31 ( Mm01242584_m1) , U6 (001973), Interferon-β (IFN-β ; Mm00439546_s1), Interferon-γ (IFN-γ ; Mm00801778_m1), Tumor necrosis factor-α (TNF-α ; Mm00443258_m1), Interleukin-6 (IL-6 ; Mm00446190_m1), and Glyceraldehyde-3-phosphate dehydrogenase (Gapdh ; 4352932E), and each ASO strand were designed by Thermo Fisher Scientific.

    Techniques: Radioactivity, Injection, Mouse Assay, Allele-specific Oligonucleotide

    Cumulative gene silencing effect by repeated administration of Toc-HDO. ( A ) OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates after four intravenous injections (at 1-week intervals) of 13-mer ASO, HDO, or Toc-HDO (No. 18) at doses corresponding to 50 mg/kg of ASO. Brains were obtained 72 h after the last injection. Data shown are relative to CD31 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, ** P

    Journal: Scientific Reports

    Article Title: Modulation of blood-brain barrier function by a heteroduplex oligonucleotide in vivo

    doi: 10.1038/s41598-018-22577-2

    Figure Lengend Snippet: Cumulative gene silencing effect by repeated administration of Toc-HDO. ( A ) OAT3 mRNA levels measured by quantitative RT-PCR in brain homogenates after four intravenous injections (at 1-week intervals) of 13-mer ASO, HDO, or Toc-HDO (No. 18) at doses corresponding to 50 mg/kg of ASO. Brains were obtained 72 h after the last injection. Data shown are relative to CD31 mRNA levels and are expressed as mean values ± s.e.m. ( N = 3, ** P

    Article Snippet: The primers and probes for mouse OAT3 (Mm00459534_m1), Claudin-5 (Mm00727012_s1), CD31 ( Mm01242584_m1) , U6 (001973), Interferon-β (IFN-β ; Mm00439546_s1), Interferon-γ (IFN-γ ; Mm00801778_m1), Tumor necrosis factor-α (TNF-α ; Mm00443258_m1), Interleukin-6 (IL-6 ; Mm00446190_m1), and Glyceraldehyde-3-phosphate dehydrogenase (Gapdh ; 4352932E), and each ASO strand were designed by Thermo Fisher Scientific.

    Techniques: Quantitative RT-PCR, Allele-specific Oligonucleotide, Injection