light chain specificity Search Results


ip jackson immunoresearch europe  (Jackson Immuno)
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Jackson Immuno ip jackson immunoresearch europe
Ip Jackson Immunoresearch Europe, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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light chain specific antibody  (Proteintech)
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Proteintech light chain specific antibody
Light Chain Specific Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti mouse igg hrp  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc goat anti mouse igg hrp
Goat Anti Mouse Igg Hrp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lc3b  (Proteintech)
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Proteintech lc3b
Fig. 3. miR-93-5p affects macrophage autophagy function by affecting Atg16L1. (A) Nine mRNAs were identifed based based on TargetScan, miRDB, miRTarBase and Human Autophage Database. (B) Schematic representation of the wild-type and mutant-type binding site between the 3′UTR of ATG16L1 and miR-93-5p. (C) Relative luciferase activity of 3′UTR-ATG16L1-luc constructs in HEK293T cells after transfection of miR-93-5p mimics/NC; n = 3 per group. (D) The protein levels of ATG16L1 in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS (Control) by Western blot. (E) The protein levels of ATG16L1 in skin tissues from normal or diabetic wound (DFUs) by Western blot. (F) Immunofluorescence staining for ATG16L1 (red) and F4/80 (green) in skin tissues from normal or diabetic wound (DFUs); scale bar, 100 μm. (G) The protein levels of <t>LC3b</t> and P62 in BMDMs transfected with miR-93-5p NC/mimics or PBS by Western blot, immunofluorescence staining for LC3b (green) in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS; scale bar, 50 μm. (H) The protein levels of ATG16L1, LC3 and P62 in fibroblast-derived exosomes treated bone marrow-derived macrophages transfected with miR-93-5p inhibitor NC/in hibitor or PBS (Control) by Western blot. Data are expressed as mean ± SD, ns P > 0.05, **P < 0.01 (unpaired t-test or ANOVA). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Lc3b, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hrp  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc hrp
Fig. 3. miR-93-5p affects macrophage autophagy function by affecting Atg16L1. (A) Nine mRNAs were identifed based based on TargetScan, miRDB, miRTarBase and Human Autophage Database. (B) Schematic representation of the wild-type and mutant-type binding site between the 3′UTR of ATG16L1 and miR-93-5p. (C) Relative luciferase activity of 3′UTR-ATG16L1-luc constructs in HEK293T cells after transfection of miR-93-5p mimics/NC; n = 3 per group. (D) The protein levels of ATG16L1 in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS (Control) by Western blot. (E) The protein levels of ATG16L1 in skin tissues from normal or diabetic wound (DFUs) by Western blot. (F) Immunofluorescence staining for ATG16L1 (red) and F4/80 (green) in skin tissues from normal or diabetic wound (DFUs); scale bar, 100 μm. (G) The protein levels of <t>LC3b</t> and P62 in BMDMs transfected with miR-93-5p NC/mimics or PBS by Western blot, immunofluorescence staining for LC3b (green) in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS; scale bar, 50 μm. (H) The protein levels of ATG16L1, LC3 and P62 in fibroblast-derived exosomes treated bone marrow-derived macrophages transfected with miR-93-5p inhibitor NC/in hibitor or PBS (Control) by Western blot. Data are expressed as mean ± SD, ns P > 0.05, **P < 0.01 (unpaired t-test or ANOVA). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Hrp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peroxidase conjugated igg fraction monoclonal mouse anti rabbit  (Jackson Immuno)
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Jackson Immuno peroxidase conjugated igg fraction monoclonal mouse anti rabbit
Fig. 3. miR-93-5p affects macrophage autophagy function by affecting Atg16L1. (A) Nine mRNAs were identifed based based on TargetScan, miRDB, miRTarBase and Human Autophage Database. (B) Schematic representation of the wild-type and mutant-type binding site between the 3′UTR of ATG16L1 and miR-93-5p. (C) Relative luciferase activity of 3′UTR-ATG16L1-luc constructs in HEK293T cells after transfection of miR-93-5p mimics/NC; n = 3 per group. (D) The protein levels of ATG16L1 in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS (Control) by Western blot. (E) The protein levels of ATG16L1 in skin tissues from normal or diabetic wound (DFUs) by Western blot. (F) Immunofluorescence staining for ATG16L1 (red) and F4/80 (green) in skin tissues from normal or diabetic wound (DFUs); scale bar, 100 μm. (G) The protein levels of <t>LC3b</t> and P62 in BMDMs transfected with miR-93-5p NC/mimics or PBS by Western blot, immunofluorescence staining for LC3b (green) in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS; scale bar, 50 μm. (H) The protein levels of ATG16L1, LC3 and P62 in fibroblast-derived exosomes treated bone marrow-derived macrophages transfected with miR-93-5p inhibitor NC/in hibitor or PBS (Control) by Western blot. Data are expressed as mean ± SD, ns P > 0.05, **P < 0.01 (unpaired t-test or ANOVA). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Peroxidase Conjugated Igg Fraction Monoclonal Mouse Anti Rabbit, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti mouse igg heavy light chain specific alkaline phosphatase conjugated antibody  (Jackson Immuno)
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Jackson Immuno goat anti mouse igg heavy light chain specific alkaline phosphatase conjugated antibody
Fig. 3. miR-93-5p affects macrophage autophagy function by affecting Atg16L1. (A) Nine mRNAs were identifed based based on TargetScan, miRDB, miRTarBase and Human Autophage Database. (B) Schematic representation of the wild-type and mutant-type binding site between the 3′UTR of ATG16L1 and miR-93-5p. (C) Relative luciferase activity of 3′UTR-ATG16L1-luc constructs in HEK293T cells after transfection of miR-93-5p mimics/NC; n = 3 per group. (D) The protein levels of ATG16L1 in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS (Control) by Western blot. (E) The protein levels of ATG16L1 in skin tissues from normal or diabetic wound (DFUs) by Western blot. (F) Immunofluorescence staining for ATG16L1 (red) and F4/80 (green) in skin tissues from normal or diabetic wound (DFUs); scale bar, 100 μm. (G) The protein levels of <t>LC3b</t> and P62 in BMDMs transfected with miR-93-5p NC/mimics or PBS by Western blot, immunofluorescence staining for LC3b (green) in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS; scale bar, 50 μm. (H) The protein levels of ATG16L1, LC3 and P62 in fibroblast-derived exosomes treated bone marrow-derived macrophages transfected with miR-93-5p inhibitor NC/in hibitor or PBS (Control) by Western blot. Data are expressed as mean ± SD, ns P > 0.05, **P < 0.01 (unpaired t-test or ANOVA). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Goat Anti Mouse Igg Heavy Light Chain Specific Alkaline Phosphatase Conjugated Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa fluor 680 affinipure goat anti mouse igg light chain  (Jackson Immuno)
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Jackson Immuno alexa fluor 680 affinipure goat anti mouse igg light chain
Fig. 3. miR-93-5p affects macrophage autophagy function by affecting Atg16L1. (A) Nine mRNAs were identifed based based on TargetScan, miRDB, miRTarBase and Human Autophage Database. (B) Schematic representation of the wild-type and mutant-type binding site between the 3′UTR of ATG16L1 and miR-93-5p. (C) Relative luciferase activity of 3′UTR-ATG16L1-luc constructs in HEK293T cells after transfection of miR-93-5p mimics/NC; n = 3 per group. (D) The protein levels of ATG16L1 in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS (Control) by Western blot. (E) The protein levels of ATG16L1 in skin tissues from normal or diabetic wound (DFUs) by Western blot. (F) Immunofluorescence staining for ATG16L1 (red) and F4/80 (green) in skin tissues from normal or diabetic wound (DFUs); scale bar, 100 μm. (G) The protein levels of <t>LC3b</t> and P62 in BMDMs transfected with miR-93-5p NC/mimics or PBS by Western blot, immunofluorescence staining for LC3b (green) in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS; scale bar, 50 μm. (H) The protein levels of ATG16L1, LC3 and P62 in fibroblast-derived exosomes treated bone marrow-derived macrophages transfected with miR-93-5p inhibitor NC/in hibitor or PBS (Control) by Western blot. Data are expressed as mean ± SD, ns P > 0.05, **P < 0.01 (unpaired t-test or ANOVA). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Alexa Fluor 680 Affinipure Goat Anti Mouse Igg Light Chain, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti rat igg  (Cell Signaling Technology Inc)
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Fig. 3. miR-93-5p affects macrophage autophagy function by affecting Atg16L1. (A) Nine mRNAs were identifed based based on TargetScan, miRDB, miRTarBase and Human Autophage Database. (B) Schematic representation of the wild-type and mutant-type binding site between the 3′UTR of ATG16L1 and miR-93-5p. (C) Relative luciferase activity of 3′UTR-ATG16L1-luc constructs in HEK293T cells after transfection of miR-93-5p mimics/NC; n = 3 per group. (D) The protein levels of ATG16L1 in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS (Control) by Western blot. (E) The protein levels of ATG16L1 in skin tissues from normal or diabetic wound (DFUs) by Western blot. (F) Immunofluorescence staining for ATG16L1 (red) and F4/80 (green) in skin tissues from normal or diabetic wound (DFUs); scale bar, 100 μm. (G) The protein levels of <t>LC3b</t> and P62 in BMDMs transfected with miR-93-5p NC/mimics or PBS by Western blot, immunofluorescence staining for LC3b (green) in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS; scale bar, 50 μm. (H) The protein levels of ATG16L1, LC3 and P62 in fibroblast-derived exosomes treated bone marrow-derived macrophages transfected with miR-93-5p inhibitor NC/in hibitor or PBS (Control) by Western blot. Data are expressed as mean ± SD, ns P > 0.05, **P < 0.01 (unpaired t-test or ANOVA). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Goat Anti Rat Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti mouse total mlc ab  (Cell Signaling Technology Inc)
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Fig. 3. miR-93-5p affects macrophage autophagy function by affecting Atg16L1. (A) Nine mRNAs were identifed based based on TargetScan, miRDB, miRTarBase and Human Autophage Database. (B) Schematic representation of the wild-type and mutant-type binding site between the 3′UTR of ATG16L1 and miR-93-5p. (C) Relative luciferase activity of 3′UTR-ATG16L1-luc constructs in HEK293T cells after transfection of miR-93-5p mimics/NC; n = 3 per group. (D) The protein levels of ATG16L1 in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS (Control) by Western blot. (E) The protein levels of ATG16L1 in skin tissues from normal or diabetic wound (DFUs) by Western blot. (F) Immunofluorescence staining for ATG16L1 (red) and F4/80 (green) in skin tissues from normal or diabetic wound (DFUs); scale bar, 100 μm. (G) The protein levels of <t>LC3b</t> and P62 in BMDMs transfected with miR-93-5p NC/mimics or PBS by Western blot, immunofluorescence staining for LC3b (green) in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS; scale bar, 50 μm. (H) The protein levels of ATG16L1, LC3 and P62 in fibroblast-derived exosomes treated bone marrow-derived macrophages transfected with miR-93-5p inhibitor NC/in hibitor or PBS (Control) by Western blot. Data are expressed as mean ± SD, ns P > 0.05, **P < 0.01 (unpaired t-test or ANOVA). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Rabbit Anti Mouse Total Mlc Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti mouse dylight 594  (Jackson Immuno)
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Fig. 3. miR-93-5p affects macrophage autophagy function by affecting Atg16L1. (A) Nine mRNAs were identifed based based on TargetScan, miRDB, miRTarBase and Human Autophage Database. (B) Schematic representation of the wild-type and mutant-type binding site between the 3′UTR of ATG16L1 and miR-93-5p. (C) Relative luciferase activity of 3′UTR-ATG16L1-luc constructs in HEK293T cells after transfection of miR-93-5p mimics/NC; n = 3 per group. (D) The protein levels of ATG16L1 in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS (Control) by Western blot. (E) The protein levels of ATG16L1 in skin tissues from normal or diabetic wound (DFUs) by Western blot. (F) Immunofluorescence staining for ATG16L1 (red) and F4/80 (green) in skin tissues from normal or diabetic wound (DFUs); scale bar, 100 μm. (G) The protein levels of <t>LC3b</t> and P62 in BMDMs transfected with miR-93-5p NC/mimics or PBS by Western blot, immunofluorescence staining for LC3b (green) in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS; scale bar, 50 μm. (H) The protein levels of ATG16L1, LC3 and P62 in fibroblast-derived exosomes treated bone marrow-derived macrophages transfected with miR-93-5p inhibitor NC/in hibitor or PBS (Control) by Western blot. Data are expressed as mean ± SD, ns P > 0.05, **P < 0.01 (unpaired t-test or ANOVA). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Goat Anti Mouse Dylight 594, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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secondary dylight 488 conjugated goat anti mouse igg antibodies  (Jackson Immuno)
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Fig. 3. miR-93-5p affects macrophage autophagy function by affecting Atg16L1. (A) Nine mRNAs were identifed based based on TargetScan, miRDB, miRTarBase and Human Autophage Database. (B) Schematic representation of the wild-type and mutant-type binding site between the 3′UTR of ATG16L1 and miR-93-5p. (C) Relative luciferase activity of 3′UTR-ATG16L1-luc constructs in HEK293T cells after transfection of miR-93-5p mimics/NC; n = 3 per group. (D) The protein levels of ATG16L1 in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS (Control) by Western blot. (E) The protein levels of ATG16L1 in skin tissues from normal or diabetic wound (DFUs) by Western blot. (F) Immunofluorescence staining for ATG16L1 (red) and F4/80 (green) in skin tissues from normal or diabetic wound (DFUs); scale bar, 100 μm. (G) The protein levels of <t>LC3b</t> and P62 in BMDMs transfected with miR-93-5p NC/mimics or PBS by Western blot, immunofluorescence staining for LC3b (green) in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS; scale bar, 50 μm. (H) The protein levels of ATG16L1, LC3 and P62 in fibroblast-derived exosomes treated bone marrow-derived macrophages transfected with miR-93-5p inhibitor NC/in hibitor or PBS (Control) by Western blot. Data are expressed as mean ± SD, ns P > 0.05, **P < 0.01 (unpaired t-test or ANOVA). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Secondary Dylight 488 Conjugated Goat Anti Mouse Igg Antibodies, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 3. miR-93-5p affects macrophage autophagy function by affecting Atg16L1. (A) Nine mRNAs were identifed based based on TargetScan, miRDB, miRTarBase and Human Autophage Database. (B) Schematic representation of the wild-type and mutant-type binding site between the 3′UTR of ATG16L1 and miR-93-5p. (C) Relative luciferase activity of 3′UTR-ATG16L1-luc constructs in HEK293T cells after transfection of miR-93-5p mimics/NC; n = 3 per group. (D) The protein levels of ATG16L1 in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS (Control) by Western blot. (E) The protein levels of ATG16L1 in skin tissues from normal or diabetic wound (DFUs) by Western blot. (F) Immunofluorescence staining for ATG16L1 (red) and F4/80 (green) in skin tissues from normal or diabetic wound (DFUs); scale bar, 100 μm. (G) The protein levels of LC3b and P62 in BMDMs transfected with miR-93-5p NC/mimics or PBS by Western blot, immunofluorescence staining for LC3b (green) in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS; scale bar, 50 μm. (H) The protein levels of ATG16L1, LC3 and P62 in fibroblast-derived exosomes treated bone marrow-derived macrophages transfected with miR-93-5p inhibitor NC/in hibitor or PBS (Control) by Western blot. Data are expressed as mean ± SD, ns P > 0.05, **P < 0.01 (unpaired t-test or ANOVA). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Exosomes derived from fibroblasts in DFUs delay wound healing by delivering miR-93-5p to target macrophage ATG16L1.

doi: 10.1016/j.bbadis.2024.167640

Figure Lengend Snippet: Fig. 3. miR-93-5p affects macrophage autophagy function by affecting Atg16L1. (A) Nine mRNAs were identifed based based on TargetScan, miRDB, miRTarBase and Human Autophage Database. (B) Schematic representation of the wild-type and mutant-type binding site between the 3′UTR of ATG16L1 and miR-93-5p. (C) Relative luciferase activity of 3′UTR-ATG16L1-luc constructs in HEK293T cells after transfection of miR-93-5p mimics/NC; n = 3 per group. (D) The protein levels of ATG16L1 in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS (Control) by Western blot. (E) The protein levels of ATG16L1 in skin tissues from normal or diabetic wound (DFUs) by Western blot. (F) Immunofluorescence staining for ATG16L1 (red) and F4/80 (green) in skin tissues from normal or diabetic wound (DFUs); scale bar, 100 μm. (G) The protein levels of LC3b and P62 in BMDMs transfected with miR-93-5p NC/mimics or PBS by Western blot, immunofluorescence staining for LC3b (green) in bone marrow-derived macrophages transfected with miR-93-5p NC/mimics or PBS; scale bar, 50 μm. (H) The protein levels of ATG16L1, LC3 and P62 in fibroblast-derived exosomes treated bone marrow-derived macrophages transfected with miR-93-5p inhibitor NC/in hibitor or PBS (Control) by Western blot. Data are expressed as mean ± SD, ns P > 0.05, **P < 0.01 (unpaired t-test or ANOVA). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Next, sections were incubated overnight at 4 ◦C with primary antibodies against F4/80 (Servicebio, GB11027), Atg16L1 (CST, #8089), LC3B (Proteintech, 81004-1-RR), α-SMA (Proteintech,14395-1AP) and Collagen Type I (Proteintech, 14695-1-AP).

Techniques: Mutagenesis, Binding Assay, Luciferase, Activity Assay, Construct, Transfection, Derivative Assay, Control, Western Blot, Immunofluorescence, Staining