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  • 99
    New England Biolabs ligation
    Ligation, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1396 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ligation/product/New England Biolabs
    Average 99 stars, based on 1396 article reviews
    Price from $9.99 to $1999.99
    ligation - by Bioz Stars, 2020-07
    99/100 stars
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    99
    New England Biolabs nebnext ultra ligation kit
    Example of yield measurements obtained with the <t>NEBNext</t> kit when comparing the amount of DNA at each step versus the initial DNA input (500 ng). Blue bars correspond to values measured using the PhiX specific primers and reflect the DNA loss at each step mainly due to pipetting and bead clean up. The orange bar corresponds to the value measured with the adaptor specific primers and reflects the amount of DNA in the sample bearing adaptor after the ligation step in comparison with the initial DNA input. The green bar corresponds to the value measured with the P5/P7 primers and reflects the amount of sequencable DNA in the sample at the end of the library preparation
    Nebnext Ultra Ligation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra ligation kit/product/New England Biolabs
    Average 99 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra ligation kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs nebnext quick ligation module
    Example of yield measurements obtained with the <t>NEBNext</t> kit when comparing the amount of DNA at each step versus the initial DNA input (500 ng). Blue bars correspond to values measured using the PhiX specific primers and reflect the DNA loss at each step mainly due to pipetting and bead clean up. The orange bar corresponds to the value measured with the adaptor specific primers and reflects the amount of DNA in the sample bearing adaptor after the ligation step in comparison with the initial DNA input. The green bar corresponds to the value measured with the P5/P7 primers and reflects the amount of sequencable DNA in the sample at the end of the library preparation
    Nebnext Quick Ligation Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext quick ligation module/product/New England Biolabs
    Average 99 stars, based on 484 article reviews
    Price from $9.99 to $1999.99
    nebnext quick ligation module - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs nebnext adaptor ligation kit
    Example of yield measurements obtained with the <t>NEBNext</t> kit when comparing the amount of DNA at each step versus the initial DNA input (500 ng). Blue bars correspond to values measured using the PhiX specific primers and reflect the DNA loss at each step mainly due to pipetting and bead clean up. The orange bar corresponds to the value measured with the adaptor specific primers and reflects the amount of DNA in the sample bearing adaptor after the ligation step in comparison with the initial DNA input. The green bar corresponds to the value measured with the P5/P7 primers and reflects the amount of sequencable DNA in the sample at the end of the library preparation
    Nebnext Adaptor Ligation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext adaptor ligation kit/product/New England Biolabs
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    nebnext adaptor ligation kit - by Bioz Stars, 2020-07
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    99
    New England Biolabs nebnext ultra 2 ligation kit
    Agarose-gel electrophoresis of gDNA fragmented by the <t>FTP</t> method. gDNA of E . coli BL21 was incubated as described in Materials and Methods: without enzymes (lane 1), with SD polymerase (lane 2), with DNase I (lane 3), and with both DNase I and SD polymerase (lane 4 and 5). M1: 1 kb <t>DNA</t> Ladder; M2: 100 bp DNA Ladder.
    Nebnext Ultra 2 Ligation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra 2 ligation kit/product/New England Biolabs
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra 2 ligation kit - by Bioz Stars, 2020-07
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      Buy from Supplier

    93
    New England Biolabs sequencing kit
    Agarose-gel electrophoresis of gDNA fragmented by the <t>FTP</t> method. gDNA of E . coli BL21 was incubated as described in Materials and Methods: without enzymes (lane 1), with SD polymerase (lane 2), with DNase I (lane 3), and with both DNase I and SD polymerase (lane 4 and 5). M1: 1 kb <t>DNA</t> Ladder; M2: 100 bp DNA Ladder.
    Sequencing Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequencing kit/product/New England Biolabs
    Average 93 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    sequencing kit - by Bioz Stars, 2020-07
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    Image Search Results


    Example of yield measurements obtained with the NEBNext kit when comparing the amount of DNA at each step versus the initial DNA input (500 ng). Blue bars correspond to values measured using the PhiX specific primers and reflect the DNA loss at each step mainly due to pipetting and bead clean up. The orange bar corresponds to the value measured with the adaptor specific primers and reflects the amount of DNA in the sample bearing adaptor after the ligation step in comparison with the initial DNA input. The green bar corresponds to the value measured with the P5/P7 primers and reflects the amount of sequencable DNA in the sample at the end of the library preparation

    Journal: BMC Genomics

    Article Title: Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing

    doi: 10.1186/s12864-016-2757-4

    Figure Lengend Snippet: Example of yield measurements obtained with the NEBNext kit when comparing the amount of DNA at each step versus the initial DNA input (500 ng). Blue bars correspond to values measured using the PhiX specific primers and reflect the DNA loss at each step mainly due to pipetting and bead clean up. The orange bar corresponds to the value measured with the adaptor specific primers and reflects the amount of DNA in the sample bearing adaptor after the ligation step in comparison with the initial DNA input. The green bar corresponds to the value measured with the P5/P7 primers and reflects the amount of sequencable DNA in the sample at the end of the library preparation

    Article Snippet: DNA library preparation kits for Illumina sequencing In this study, the following kits were tested and compared: NEBNext and NEBNext Ultra from NEB, SureSelectXT from Agilent, Truseq Nano and Truseq DNA PCR-free from Illumina, Accel-NGS 1S and 2S from Swift Biosciences, and KAPA Hyper and KAPA HyperPlus from KAPA Biosystems (see Table ).

    Techniques: Ligation

    Agarose-gel electrophoresis of gDNA fragmented by the FTP method. gDNA of E . coli BL21 was incubated as described in Materials and Methods: without enzymes (lane 1), with SD polymerase (lane 2), with DNase I (lane 3), and with both DNase I and SD polymerase (lane 4 and 5). M1: 1 kb DNA Ladder; M2: 100 bp DNA Ladder.

    Journal: PLoS ONE

    Article Title: Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing

    doi: 10.1371/journal.pone.0210374

    Figure Lengend Snippet: Agarose-gel electrophoresis of gDNA fragmented by the FTP method. gDNA of E . coli BL21 was incubated as described in Materials and Methods: without enzymes (lane 1), with SD polymerase (lane 2), with DNase I (lane 3), and with both DNase I and SD polymerase (lane 4 and 5). M1: 1 kb DNA Ladder; M2: 100 bp DNA Ladder.

    Article Snippet: NGS libraries from FTP digested gDNA were constructed using the NEBNext Ultra II DNA Library Prep Kit procedure, excluding the DNA end repair stage.

    Techniques: Agarose Gel Electrophoresis, Incubation

    A general overview of the dsDNA Fragmentation Through Polymerization (FTP) method. The FTP method is based on two enzymatic reactions: a DNA nicking reaction with DNase I and a strand-displacement DNA polymerization with SD DNA polymerase. As a result, multiple double-stranded DNA fragments with overlapping sequences are generated. De novo synthesized DNA is indicated in grey, and SD polymerase is indicated in red.

    Journal: PLoS ONE

    Article Title: Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing

    doi: 10.1371/journal.pone.0210374

    Figure Lengend Snippet: A general overview of the dsDNA Fragmentation Through Polymerization (FTP) method. The FTP method is based on two enzymatic reactions: a DNA nicking reaction with DNase I and a strand-displacement DNA polymerization with SD DNA polymerase. As a result, multiple double-stranded DNA fragments with overlapping sequences are generated. De novo synthesized DNA is indicated in grey, and SD polymerase is indicated in red.

    Article Snippet: NGS libraries from FTP digested gDNA were constructed using the NEBNext Ultra II DNA Library Prep Kit procedure, excluding the DNA end repair stage.

    Techniques: Generated, Synthesized