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  • 99
    Thermo Fisher museek library preparation kit
    Museek Library Preparation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/museek library preparation kit/product/Thermo Fisher
    Average 99 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    museek library preparation kit - by Bioz Stars, 2020-04
    99/100 stars
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    94
    Thermo Fisher claseek library preparation kit
    Claseek Library Preparation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/claseek library preparation kit/product/Thermo Fisher
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    claseek library preparation kit - by Bioz Stars, 2020-04
    94/100 stars
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    94
    Roche library preparation kit
    Library Preparation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/library preparation kit/product/Roche
    Average 94 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    library preparation kit - by Bioz Stars, 2020-04
    94/100 stars
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    94
    Diagenode library preparation kit
    Library Preparation Kit, supplied by Diagenode, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/library preparation kit/product/Diagenode
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    library preparation kit - by Bioz Stars, 2020-04
    94/100 stars
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    92
    Kapa Biosystems library preparation kit
    Library Preparation Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 92/100, based on 236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/library preparation kit/product/Kapa Biosystems
    Average 92 stars, based on 236 article reviews
    Price from $9.99 to $1999.99
    library preparation kit - by Bioz Stars, 2020-04
    92/100 stars
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    97
    Illumina Inc library preparation kits
    Library Preparation Kits, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/library preparation kits/product/Illumina Inc
    Average 97 stars, based on 163 article reviews
    Price from $9.99 to $1999.99
    library preparation kits - by Bioz Stars, 2020-04
    97/100 stars
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    99
    Illumina Inc truseq library preparation kits
    Number of HiSeq reads returned for libraries prepared using <t>Illumina</t> <t>TruSeq</t> adapters versus libraries prepared using adapters integrating edit metric sequence tags designed using EDITTAG.
    Truseq Library Preparation Kits, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq library preparation kits/product/Illumina Inc
    Average 99 stars, based on 145 article reviews
    Price from $9.99 to $1999.99
    truseq library preparation kits - by Bioz Stars, 2020-04
    99/100 stars
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    94
    Kapa Biosystems hyperprep library preparation kits
    Number of HiSeq reads returned for libraries prepared using <t>Illumina</t> <t>TruSeq</t> adapters versus libraries prepared using adapters integrating edit metric sequence tags designed using EDITTAG.
    Hyperprep Library Preparation Kits, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hyperprep library preparation kits/product/Kapa Biosystems
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hyperprep library preparation kits - by Bioz Stars, 2020-04
    94/100 stars
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    94
    Kapa Biosystems ltp library preparation kit
    Number of HiSeq reads returned for libraries prepared using <t>Illumina</t> <t>TruSeq</t> adapters versus libraries prepared using adapters integrating edit metric sequence tags designed using EDITTAG.
    Ltp Library Preparation Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltp library preparation kit/product/Kapa Biosystems
    Average 94 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    ltp library preparation kit - by Bioz Stars, 2020-04
    94/100 stars
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    99
    Diagenode microplex library preparation kit
    Length distribution of interrupted palindromes at 5′ and 3′-ends in Illumina HiSeq 2000 reads of Atlantic cod ( Gadus morhua ). Reads were generated from 11 historic samples using TruSeq library creation protocols (red lines), four historic samples using <t>Microplex</t> protocols (black lines) and one modern sample using TruSeq protocols (grey line). Terminal palindromic sequences longer than three basepair are rare in the Microplex and modern samples.
    Microplex Library Preparation Kit, supplied by Diagenode, used in various techniques. Bioz Stars score: 99/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microplex library preparation kit/product/Diagenode
    Average 99 stars, based on 319 article reviews
    Price from $9.99 to $1999.99
    microplex library preparation kit - by Bioz Stars, 2020-04
    99/100 stars
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    94
    WaferGen Biosystems prepx library preparation kit
    Length distribution of interrupted palindromes at 5′ and 3′-ends in Illumina HiSeq 2000 reads of Atlantic cod ( Gadus morhua ). Reads were generated from 11 historic samples using TruSeq library creation protocols (red lines), four historic samples using <t>Microplex</t> protocols (black lines) and one modern sample using TruSeq protocols (grey line). Terminal palindromic sequences longer than three basepair are rare in the Microplex and modern samples.
    Prepx Library Preparation Kit, supplied by WaferGen Biosystems, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prepx library preparation kit/product/WaferGen Biosystems
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    prepx library preparation kit - by Bioz Stars, 2020-04
    94/100 stars
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    98
    Kapa Biosystems hyper library preparation kit
    Length distribution of interrupted palindromes at 5′ and 3′-ends in Illumina HiSeq 2000 reads of Atlantic cod ( Gadus morhua ). Reads were generated from 11 historic samples using TruSeq library creation protocols (red lines), four historic samples using <t>Microplex</t> protocols (black lines) and one modern sample using TruSeq protocols (grey line). Terminal palindromic sequences longer than three basepair are rare in the Microplex and modern samples.
    Hyper Library Preparation Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 98/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hyper library preparation kit/product/Kapa Biosystems
    Average 98 stars, based on 84 article reviews
    Price from $9.99 to $1999.99
    hyper library preparation kit - by Bioz Stars, 2020-04
    98/100 stars
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    92
    Roche rapid library preparation kit
    Length distribution of interrupted palindromes at 5′ and 3′-ends in Illumina HiSeq 2000 reads of Atlantic cod ( Gadus morhua ). Reads were generated from 11 historic samples using TruSeq library creation protocols (red lines), four historic samples using <t>Microplex</t> protocols (black lines) and one modern sample using TruSeq protocols (grey line). Terminal palindromic sequences longer than three basepair are rare in the Microplex and modern samples.
    Rapid Library Preparation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid library preparation kit/product/Roche
    Average 92 stars, based on 121 article reviews
    Price from $9.99 to $1999.99
    rapid library preparation kit - by Bioz Stars, 2020-04
    92/100 stars
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    96
    Genewiz metavx library preparation kit
    Length distribution of interrupted palindromes at 5′ and 3′-ends in Illumina HiSeq 2000 reads of Atlantic cod ( Gadus morhua ). Reads were generated from 11 historic samples using TruSeq library creation protocols (red lines), four historic samples using <t>Microplex</t> protocols (black lines) and one modern sample using TruSeq protocols (grey line). Terminal palindromic sequences longer than three basepair are rare in the Microplex and modern samples.
    Metavx Library Preparation Kit, supplied by Genewiz, used in various techniques. Bioz Stars score: 96/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/metavx library preparation kit/product/Genewiz
    Average 96 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    metavx library preparation kit - by Bioz Stars, 2020-04
    96/100 stars
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    99
    Illumina Inc nexteraxt library preparation kit
    Experimental scheme. ( A ) After differentiation, the cell cultures were dissociated to single-cell suspensions and loaded onto a Fluidigm C1 Single-Cell Auto Prep Array for mRNA-Seq. On the arrays, the cells were lysed, and reverse transcription of the mRNA and PCR amplification of the cDNA were performed using the C1 Single-Cell Auto Prep System (Fluidigm). Libraries were then prepared using the <t>Nextera</t> XT DNA Library Prep Kit and mosquito HTS liquid handler (TTP). For the final PCR reactions, we used a Bio-Rad 384 Thermal Cycler. Libraries were pooled and sequenced on an Illumina HiSeq 2500. ( B ) WA09 human embryonic stem cells were differentiated in vitro to the pancreatic lineage. Cells from stages 1 and 2 were collected and analyzed using the procedures outlined in A . Two independent cells from stage 1 (cell A and cell B) and two cells from stage 2 (cell C and cell D) were selected and yielded with similar cDNA concentrations (mean [SD] concentration = 0.38 [0.04] ng/µL). For library preparation, we tested 2-µL, 4-µL, and 8-µL final volume reactions, with four replicates per reaction volume.
    Nexteraxt Library Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nexteraxt library preparation kit/product/Illumina Inc
    Average 99 stars, based on 164 article reviews
    Price from $9.99 to $1999.99
    nexteraxt library preparation kit - by Bioz Stars, 2020-04
    99/100 stars
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    99
    Illumina Inc kapa library preparation kit
    Experimental scheme. ( A ) After differentiation, the cell cultures were dissociated to single-cell suspensions and loaded onto a Fluidigm C1 Single-Cell Auto Prep Array for mRNA-Seq. On the arrays, the cells were lysed, and reverse transcription of the mRNA and PCR amplification of the cDNA were performed using the C1 Single-Cell Auto Prep System (Fluidigm). Libraries were then prepared using the <t>Nextera</t> XT DNA Library Prep Kit and mosquito HTS liquid handler (TTP). For the final PCR reactions, we used a Bio-Rad 384 Thermal Cycler. Libraries were pooled and sequenced on an Illumina HiSeq 2500. ( B ) WA09 human embryonic stem cells were differentiated in vitro to the pancreatic lineage. Cells from stages 1 and 2 were collected and analyzed using the procedures outlined in A . Two independent cells from stage 1 (cell A and cell B) and two cells from stage 2 (cell C and cell D) were selected and yielded with similar cDNA concentrations (mean [SD] concentration = 0.38 [0.04] ng/µL). For library preparation, we tested 2-µL, 4-µL, and 8-µL final volume reactions, with four replicates per reaction volume.
    Kapa Library Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa library preparation kit/product/Illumina Inc
    Average 99 stars, based on 204 article reviews
    Price from $9.99 to $1999.99
    kapa library preparation kit - by Bioz Stars, 2020-04
    99/100 stars
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    98
    Roche kapa library preparation kit
    Experimental scheme. ( A ) After differentiation, the cell cultures were dissociated to single-cell suspensions and loaded onto a Fluidigm C1 Single-Cell Auto Prep Array for mRNA-Seq. On the arrays, the cells were lysed, and reverse transcription of the mRNA and PCR amplification of the cDNA were performed using the C1 Single-Cell Auto Prep System (Fluidigm). Libraries were then prepared using the <t>Nextera</t> XT DNA Library Prep Kit and mosquito HTS liquid handler (TTP). For the final PCR reactions, we used a Bio-Rad 384 Thermal Cycler. Libraries were pooled and sequenced on an Illumina HiSeq 2500. ( B ) WA09 human embryonic stem cells were differentiated in vitro to the pancreatic lineage. Cells from stages 1 and 2 were collected and analyzed using the procedures outlined in A . Two independent cells from stage 1 (cell A and cell B) and two cells from stage 2 (cell C and cell D) were selected and yielded with similar cDNA concentrations (mean [SD] concentration = 0.38 [0.04] ng/µL). For library preparation, we tested 2-µL, 4-µL, and 8-µL final volume reactions, with four replicates per reaction volume.
    Kapa Library Preparation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 98/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa library preparation kit/product/Roche
    Average 98 stars, based on 72 article reviews
    Price from $9.99 to $1999.99
    kapa library preparation kit - by Bioz Stars, 2020-04
    98/100 stars
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    95
    Genewiz metavxtm library preparation kit
    Experimental scheme. ( A ) After differentiation, the cell cultures were dissociated to single-cell suspensions and loaded onto a Fluidigm C1 Single-Cell Auto Prep Array for mRNA-Seq. On the arrays, the cells were lysed, and reverse transcription of the mRNA and PCR amplification of the cDNA were performed using the C1 Single-Cell Auto Prep System (Fluidigm). Libraries were then prepared using the <t>Nextera</t> XT DNA Library Prep Kit and mosquito HTS liquid handler (TTP). For the final PCR reactions, we used a Bio-Rad 384 Thermal Cycler. Libraries were pooled and sequenced on an Illumina HiSeq 2500. ( B ) WA09 human embryonic stem cells were differentiated in vitro to the pancreatic lineage. Cells from stages 1 and 2 were collected and analyzed using the procedures outlined in A . Two independent cells from stage 1 (cell A and cell B) and two cells from stage 2 (cell C and cell D) were selected and yielded with similar cDNA concentrations (mean [SD] concentration = 0.38 [0.04] ng/µL). For library preparation, we tested 2-µL, 4-µL, and 8-µL final volume reactions, with four replicates per reaction volume.
    Metavxtm Library Preparation Kit, supplied by Genewiz, used in various techniques. Bioz Stars score: 95/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/metavxtm library preparation kit/product/Genewiz
    Average 95 stars, based on 92 article reviews
    Price from $9.99 to $1999.99
    metavxtm library preparation kit - by Bioz Stars, 2020-04
    95/100 stars
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    94
    Kapa Biosystems kapa library preparation kit
    Experimental scheme. ( A ) After differentiation, the cell cultures were dissociated to single-cell suspensions and loaded onto a Fluidigm C1 Single-Cell Auto Prep Array for mRNA-Seq. On the arrays, the cells were lysed, and reverse transcription of the mRNA and PCR amplification of the cDNA were performed using the C1 Single-Cell Auto Prep System (Fluidigm). Libraries were then prepared using the <t>Nextera</t> XT DNA Library Prep Kit and mosquito HTS liquid handler (TTP). For the final PCR reactions, we used a Bio-Rad 384 Thermal Cycler. Libraries were pooled and sequenced on an Illumina HiSeq 2500. ( B ) WA09 human embryonic stem cells were differentiated in vitro to the pancreatic lineage. Cells from stages 1 and 2 were collected and analyzed using the procedures outlined in A . Two independent cells from stage 1 (cell A and cell B) and two cells from stage 2 (cell C and cell D) were selected and yielded with similar cDNA concentrations (mean [SD] concentration = 0.38 [0.04] ng/µL). For library preparation, we tested 2-µL, 4-µL, and 8-µL final volume reactions, with four replicates per reaction volume.
    Kapa Library Preparation Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 94/100, based on 591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa library preparation kit/product/Kapa Biosystems
    Average 94 stars, based on 591 article reviews
    Price from $9.99 to $1999.99
    kapa library preparation kit - by Bioz Stars, 2020-04
    94/100 stars
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    86
    Roche general library preparation kit
    Experimental scheme. ( A ) After differentiation, the cell cultures were dissociated to single-cell suspensions and loaded onto a Fluidigm C1 Single-Cell Auto Prep Array for mRNA-Seq. On the arrays, the cells were lysed, and reverse transcription of the mRNA and PCR amplification of the cDNA were performed using the C1 Single-Cell Auto Prep System (Fluidigm). Libraries were then prepared using the <t>Nextera</t> XT DNA Library Prep Kit and mosquito HTS liquid handler (TTP). For the final PCR reactions, we used a Bio-Rad 384 Thermal Cycler. Libraries were pooled and sequenced on an Illumina HiSeq 2500. ( B ) WA09 human embryonic stem cells were differentiated in vitro to the pancreatic lineage. Cells from stages 1 and 2 were collected and analyzed using the procedures outlined in A . Two independent cells from stage 1 (cell A and cell B) and two cells from stage 2 (cell C and cell D) were selected and yielded with similar cDNA concentrations (mean [SD] concentration = 0.38 [0.04] ng/µL). For library preparation, we tested 2-µL, 4-µL, and 8-µL final volume reactions, with four replicates per reaction volume.
    General Library Preparation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/general library preparation kit/product/Roche
    Average 86 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    general library preparation kit - by Bioz Stars, 2020-04
    86/100 stars
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    93
    10X Genomics gemcode library preparation kits
    Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x <t>GemCode</t> linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.
    Gemcode Library Preparation Kits, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gemcode library preparation kits/product/10X Genomics
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    gemcode library preparation kits - by Bioz Stars, 2020-04
    93/100 stars
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    95
    Illumina Inc rnaseq library preparation kit
    Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x <t>GemCode</t> linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.
    Rnaseq Library Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaseq library preparation kit/product/Illumina Inc
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    rnaseq library preparation kit - by Bioz Stars, 2020-04
    95/100 stars
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    99
    TaKaRa library preparation kit
    Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x <t>GemCode</t> linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.
    Library Preparation Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/library preparation kit/product/TaKaRa
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    library preparation kit - by Bioz Stars, 2020-04
    99/100 stars
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    95
    Millipore kapa library preparation kit
    Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x <t>GemCode</t> linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.
    Kapa Library Preparation Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa library preparation kit/product/Millipore
    Average 95 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    kapa library preparation kit - by Bioz Stars, 2020-04
    95/100 stars
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    94
    Pacific Biosciences smrtbell library preparation kit
    Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x <t>GemCode</t> linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.
    Smrtbell Library Preparation Kit, supplied by Pacific Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smrtbell library preparation kit/product/Pacific Biosciences
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    smrtbell library preparation kit - by Bioz Stars, 2020-04
    94/100 stars
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    94
    Millipore ngs library preparation kit
    Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x <t>GemCode</t> linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.
    Ngs Library Preparation Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngs library preparation kit/product/Millipore
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    ngs library preparation kit - by Bioz Stars, 2020-04
    94/100 stars
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    94
    Agilent technologies trueseq library preparation kit
    Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x <t>GemCode</t> linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.
    Trueseq Library Preparation Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trueseq library preparation kit/product/Agilent technologies
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    trueseq library preparation kit - by Bioz Stars, 2020-04
    94/100 stars
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    87
    Thermo Fisher amplicon library preparation kit
    Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x <t>GemCode</t> linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.
    Amplicon Library Preparation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplicon library preparation kit/product/Thermo Fisher
    Average 87 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    amplicon library preparation kit - by Bioz Stars, 2020-04
    87/100 stars
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    86
    Nugen rapid library preparation kit
    Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x <t>GemCode</t> linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.
    Rapid Library Preparation Kit, supplied by Nugen, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TriLink cleantag library preparation kit
    Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x <t>GemCode</t> linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.
    Cleantag Library Preparation Kit, supplied by TriLink, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleantag library preparation kit/product/TriLink
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    Illumina Inc truseq library preparation
    Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x <t>GemCode</t> linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.
    Truseq Library Preparation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 408 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq library preparation/product/Illumina Inc
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    Image Search Results


    Number of HiSeq reads returned for libraries prepared using Illumina TruSeq adapters versus libraries prepared using adapters integrating edit metric sequence tags designed using EDITTAG.

    Journal: PLoS ONE

    Article Title: Not All Sequence Tags Are Created Equal: Designing and Validating Sequence Identification Tags Robust to Indels

    doi: 10.1371/journal.pone.0042543

    Figure Lengend Snippet: Number of HiSeq reads returned for libraries prepared using Illumina TruSeq adapters versus libraries prepared using adapters integrating edit metric sequence tags designed using EDITTAG.

    Article Snippet: Pairwise Hamming distance comparisons between the 48 sequence tags provided as part of the Illumina TruSeq library preparation kits.

    Techniques: Sequencing

    Exemplary workflow of three principal methodologies for TCR library preparation. The figure depicts a simplified workflow of the library preparation procedure using multiplex PCR, targeted in-solution enrichment and 5’RACE-switch-oligo nested PCR. Multiplex PCR is suitable for both RNA and gDNA sequencing. Samples undergo cDNA synthesis and 1 or more PCR steps followed by adaptor ligation and sequencing. While the forward primers for cDNA synthesis are designed to cover all known V genes for both starting materials, the location and number of the reverse primers differs, due to introns in DNA. Target enrichment, also applicable to both gDNA and RNA, is preceded by a standard library preparation including fragmentation for gDNA or mRNA purification for RNA, followed by end-repairing, A-tailing and finally adaptor ligation. The enrichment of target sequences is then performed using RNA baits complementary to the sequence of interest. The RNA baits hybridize with molecules in the library, which are then retrieved using magnetic beads and can undergo further amplification before sequencing. Nested PCR based on the 5’RACE and switch-oligo approach (only for RNA) makes use of the incorporation of an adaptor molecule at the 5′ end of the cDNA during cDNA synthesis. The forward primer for a subsequent PCR is designed to bind to the 5′ adaptor sequence, while the reverse primer is designed to bind to the C-region of the transcript. Hence, only one primer pair is required to cover the complete spectrum of possible V genes. Subsequent nested PCRs performed in the same fashion may increase outcome specificity. Finally, adaptor ligation is performed. The procedures showed in this picture constitute only an example of the different available methods

    Journal: BMC Biotechnology

    Article Title: Overview of methodologies for T-cell receptor repertoire analysis

    doi: 10.1186/s12896-017-0379-9

    Figure Lengend Snippet: Exemplary workflow of three principal methodologies for TCR library preparation. The figure depicts a simplified workflow of the library preparation procedure using multiplex PCR, targeted in-solution enrichment and 5’RACE-switch-oligo nested PCR. Multiplex PCR is suitable for both RNA and gDNA sequencing. Samples undergo cDNA synthesis and 1 or more PCR steps followed by adaptor ligation and sequencing. While the forward primers for cDNA synthesis are designed to cover all known V genes for both starting materials, the location and number of the reverse primers differs, due to introns in DNA. Target enrichment, also applicable to both gDNA and RNA, is preceded by a standard library preparation including fragmentation for gDNA or mRNA purification for RNA, followed by end-repairing, A-tailing and finally adaptor ligation. The enrichment of target sequences is then performed using RNA baits complementary to the sequence of interest. The RNA baits hybridize with molecules in the library, which are then retrieved using magnetic beads and can undergo further amplification before sequencing. Nested PCR based on the 5’RACE and switch-oligo approach (only for RNA) makes use of the incorporation of an adaptor molecule at the 5′ end of the cDNA during cDNA synthesis. The forward primer for a subsequent PCR is designed to bind to the 5′ adaptor sequence, while the reverse primer is designed to bind to the C-region of the transcript. Hence, only one primer pair is required to cover the complete spectrum of possible V genes. Subsequent nested PCRs performed in the same fashion may increase outcome specificity. Finally, adaptor ligation is performed. The procedures showed in this picture constitute only an example of the different available methods

    Article Snippet: For starting material, gDNA or RNA is first processed with a standard sequencing library preparation kit (i.e. Illumina TruSeq or SureSelectXT from Agilent), followed by incubation of the samples with custom designed RNA baits.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Nested PCR, Sequencing, Ligation, Purification, Magnetic Beads, Amplification

    Length distribution of interrupted palindromes at 5′ and 3′-ends in Illumina HiSeq 2000 reads of Atlantic cod ( Gadus morhua ). Reads were generated from 11 historic samples using TruSeq library creation protocols (red lines), four historic samples using Microplex protocols (black lines) and one modern sample using TruSeq protocols (grey line). Terminal palindromic sequences longer than three basepair are rare in the Microplex and modern samples.

    Journal: PLoS ONE

    Article Title: Palindromic Sequence Artifacts Generated during Next Generation Sequencing Library Preparation from Historic and Ancient DNA

    doi: 10.1371/journal.pone.0089676

    Figure Lengend Snippet: Length distribution of interrupted palindromes at 5′ and 3′-ends in Illumina HiSeq 2000 reads of Atlantic cod ( Gadus morhua ). Reads were generated from 11 historic samples using TruSeq library creation protocols (red lines), four historic samples using Microplex protocols (black lines) and one modern sample using TruSeq protocols (grey line). Terminal palindromic sequences longer than three basepair are rare in the Microplex and modern samples.

    Article Snippet: The Microplex Library Preparation kit ( www.diagenode.com ) differs from the TruSeq protocol in that it is a single tube protocol, uses blunt-end ligation of stem-loop adapters, and no intermediate purification procedures are used before library amplification.

    Techniques: Generated

    Proportion of reads aligning to the Atlantic cod genome for TruSeq and Microplex libraries. The proportions of reads aligning (relative to the number of untrimmed read pairs) were calculated for libraries including interrupted palindromes (light grey) and those for which these palindromes (dark gray) were removed at the 3′-end. Only reads with a minimum mapping quality (MapQ) value of 25 were considered.

    Journal: PLoS ONE

    Article Title: Palindromic Sequence Artifacts Generated during Next Generation Sequencing Library Preparation from Historic and Ancient DNA

    doi: 10.1371/journal.pone.0089676

    Figure Lengend Snippet: Proportion of reads aligning to the Atlantic cod genome for TruSeq and Microplex libraries. The proportions of reads aligning (relative to the number of untrimmed read pairs) were calculated for libraries including interrupted palindromes (light grey) and those for which these palindromes (dark gray) were removed at the 3′-end. Only reads with a minimum mapping quality (MapQ) value of 25 were considered.

    Article Snippet: The Microplex Library Preparation kit ( www.diagenode.com ) differs from the TruSeq protocol in that it is a single tube protocol, uses blunt-end ligation of stem-loop adapters, and no intermediate purification procedures are used before library amplification.

    Techniques:

    Frequency of nucleotide substitutions along historic reads of Atlantic cod. Reads were generated using the TruSeq V2 library creation protocol ( a ) or the Microplex single tube protocol (see methods) ( b ). Misalignments to the reference at the 5′ and 3′-end of sequencing reads are the result of elevated proportions of C to T substitutions (red), G to A substitutions (blue) and other possible substitutions (grey). The figure was generated using the program mapDamage V2.0.0 using 1 million randomly chosen reads for merged Illumina and Microplex libraries [13] .

    Journal: PLoS ONE

    Article Title: Palindromic Sequence Artifacts Generated during Next Generation Sequencing Library Preparation from Historic and Ancient DNA

    doi: 10.1371/journal.pone.0089676

    Figure Lengend Snippet: Frequency of nucleotide substitutions along historic reads of Atlantic cod. Reads were generated using the TruSeq V2 library creation protocol ( a ) or the Microplex single tube protocol (see methods) ( b ). Misalignments to the reference at the 5′ and 3′-end of sequencing reads are the result of elevated proportions of C to T substitutions (red), G to A substitutions (blue) and other possible substitutions (grey). The figure was generated using the program mapDamage V2.0.0 using 1 million randomly chosen reads for merged Illumina and Microplex libraries [13] .

    Article Snippet: The Microplex Library Preparation kit ( www.diagenode.com ) differs from the TruSeq protocol in that it is a single tube protocol, uses blunt-end ligation of stem-loop adapters, and no intermediate purification procedures are used before library amplification.

    Techniques: Generated, Sequencing

    Experimental scheme. ( A ) After differentiation, the cell cultures were dissociated to single-cell suspensions and loaded onto a Fluidigm C1 Single-Cell Auto Prep Array for mRNA-Seq. On the arrays, the cells were lysed, and reverse transcription of the mRNA and PCR amplification of the cDNA were performed using the C1 Single-Cell Auto Prep System (Fluidigm). Libraries were then prepared using the Nextera XT DNA Library Prep Kit and mosquito HTS liquid handler (TTP). For the final PCR reactions, we used a Bio-Rad 384 Thermal Cycler. Libraries were pooled and sequenced on an Illumina HiSeq 2500. ( B ) WA09 human embryonic stem cells were differentiated in vitro to the pancreatic lineage. Cells from stages 1 and 2 were collected and analyzed using the procedures outlined in A . Two independent cells from stage 1 (cell A and cell B) and two cells from stage 2 (cell C and cell D) were selected and yielded with similar cDNA concentrations (mean [SD] concentration = 0.38 [0.04] ng/µL). For library preparation, we tested 2-µL, 4-µL, and 8-µL final volume reactions, with four replicates per reaction volume.

    Journal: Jala (Charlottesville, Va.)

    Article Title: Miniaturization Technologies for Efficient Single-Cell Library Preparation for Next-Generation Sequencing

    doi: 10.1177/2211068216630741

    Figure Lengend Snippet: Experimental scheme. ( A ) After differentiation, the cell cultures were dissociated to single-cell suspensions and loaded onto a Fluidigm C1 Single-Cell Auto Prep Array for mRNA-Seq. On the arrays, the cells were lysed, and reverse transcription of the mRNA and PCR amplification of the cDNA were performed using the C1 Single-Cell Auto Prep System (Fluidigm). Libraries were then prepared using the Nextera XT DNA Library Prep Kit and mosquito HTS liquid handler (TTP). For the final PCR reactions, we used a Bio-Rad 384 Thermal Cycler. Libraries were pooled and sequenced on an Illumina HiSeq 2500. ( B ) WA09 human embryonic stem cells were differentiated in vitro to the pancreatic lineage. Cells from stages 1 and 2 were collected and analyzed using the procedures outlined in A . Two independent cells from stage 1 (cell A and cell B) and two cells from stage 2 (cell C and cell D) were selected and yielded with similar cDNA concentrations (mean [SD] concentration = 0.38 [0.04] ng/µL). For library preparation, we tested 2-µL, 4-µL, and 8-µL final volume reactions, with four replicates per reaction volume.

    Article Snippet: We then used the mosquito HTS liquid handler (TTP Labtech) to complete the NGS library preparation process using the Nextera XT Library Preparation Kit (Illumina).

    Techniques: Polymerase Chain Reaction, Amplification, In Vitro, Concentration Assay

    Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x GemCode linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.

    Journal: Scientific Reports

    Article Title: Identification of potential regulatory mutations using multi-omics analysis and haplotyping of lung adenocarcinoma cell lines

    doi: 10.1038/s41598-018-23342-1

    Figure Lengend Snippet: Phasing SNVs in the EGFR gene region. ( A ) The general premise of the phasing scheme. Haplotypes were exhaustively constructed to circumvent polyploidy/aneuploidy. ( B ) A simple example of successfully phased diploid haplotypes with connecting black lines indicating the original 10x GemCode linked read (MIs; molecular indexes). ( C ) Number of linked reads/molecular indexes supporting phased block partially covering the EGFR gene region in the H1975 cell line. The dots represent 22 heterozygous SNV/indels that could be completely phased in the both alleles. Somatic SNVs T790M and L858R are shown in light green and dark green, respectively. WT: wildtype allele; MUT: T790M/L858R mutant allele. ( D ) Details of each variant covered by phase block in ( C ). Upper panel is determined by direct connection via linked read/MIs. Lower panel is inferred by gaps and content of each redundant grouping of the linked read/MIs.

    Article Snippet: End repair and A-tailing were performed by thermal cycling the fragmented DNA with the End Repair and A-Tailing Buffer and Enzyme Mix supplied in the GemCode library preparation kits (10x Genomics).

    Techniques: Construct, Blocking Assay, Mutagenesis, Variant Assay