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  • 99
    Thermo Fisher cdna synthesis leaf rna
    Cdna Synthesis Leaf Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna synthesis leaf rna/product/Thermo Fisher
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    99
    Millipore leaf rna
    Leaf Rna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore leaf total rna
    NMTase <t>mRNA</t> expression in Limonium sp. Total <t>RNA</t> (10 μg) per lane was used and probed with clone 23 DNA probe (top) or a L . latifolium actin probe (middle). The bottom panel shows an ethidium bromide-stained gel as a loading control. Mature leaf RNA was from L . latifolium (L) and L . sinuatum (S). The arrow points to 1.4-kb size.
    Leaf Total Rna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher leaf rna
    Analysis of ADK transcript abundance by <t>RNA</t> blotting and reverse transcription <t>(RT)-PCR.</t> A, Total RNA from leaves and stems of the indicated lines was probed with the full-length ADK1 cDNA. The silenced lines contain reduced ADK transcript levels in leaves and a less moderate decrease in the apparently normal secondary shoots. B, RT-PCR analysis of cDNA prepared from RNA of the indicates a reduction in ADK1 and ADK2 transcript levels in the sADK lines relative to the WT, consistent with the RNA blot results. Transcripts of RNA helicase were amplified as an internal control.
    Leaf Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Pioneer Hi-Bred leaf rna
    Analysis of ADK transcript abundance by <t>RNA</t> blotting and reverse transcription <t>(RT)-PCR.</t> A, Total RNA from leaves and stems of the indicated lines was probed with the full-length ADK1 cDNA. The silenced lines contain reduced ADK transcript levels in leaves and a less moderate decrease in the apparently normal secondary shoots. B, RT-PCR analysis of cDNA prepared from RNA of the indicates a reduction in ADK1 and ADK2 transcript levels in the sADK lines relative to the WT, consistent with the RNA blot results. Transcripts of RNA helicase were amplified as an internal control.
    Leaf Rna, supplied by Pioneer Hi-Bred, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher heinong44 leaf rna
    Analysis of ADK transcript abundance by <t>RNA</t> blotting and reverse transcription <t>(RT)-PCR.</t> A, Total RNA from leaves and stems of the indicated lines was probed with the full-length ADK1 cDNA. The silenced lines contain reduced ADK transcript levels in leaves and a less moderate decrease in the apparently normal secondary shoots. B, RT-PCR analysis of cDNA prepared from RNA of the indicates a reduction in ADK1 and ADK2 transcript levels in the sADK lines relative to the WT, consistent with the RNA blot results. Transcripts of RNA helicase were amplified as an internal control.
    Heinong44 Leaf Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    MACHEREY NAGEL leaf rna extraction
    Analysis of ADK transcript abundance by <t>RNA</t> blotting and reverse transcription <t>(RT)-PCR.</t> A, Total RNA from leaves and stems of the indicated lines was probed with the full-length ADK1 cDNA. The silenced lines contain reduced ADK transcript levels in leaves and a less moderate decrease in the apparently normal secondary shoots. B, RT-PCR analysis of cDNA prepared from RNA of the indicates a reduction in ADK1 and ADK2 transcript levels in the sADK lines relative to the WT, consistent with the RNA blot results. Transcripts of RNA helicase were amplified as an internal control.
    Leaf Rna Extraction, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher arabidopsis leaf rna
    Analysis of ADK transcript abundance by <t>RNA</t> blotting and reverse transcription <t>(RT)-PCR.</t> A, Total RNA from leaves and stems of the indicated lines was probed with the full-length ADK1 cDNA. The silenced lines contain reduced ADK transcript levels in leaves and a less moderate decrease in the apparently normal secondary shoots. B, RT-PCR analysis of cDNA prepared from RNA of the indicates a reduction in ADK1 and ADK2 transcript levels in the sADK lines relative to the WT, consistent with the RNA blot results. Transcripts of RNA helicase were amplified as an internal control.
    Arabidopsis Leaf Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Illumina Inc sequencing total leaf rna
    Analysis of ADK transcript abundance by <t>RNA</t> blotting and reverse transcription <t>(RT)-PCR.</t> A, Total RNA from leaves and stems of the indicated lines was probed with the full-length ADK1 cDNA. The silenced lines contain reduced ADK transcript levels in leaves and a less moderate decrease in the apparently normal secondary shoots. B, RT-PCR analysis of cDNA prepared from RNA of the indicates a reduction in ADK1 and ADK2 transcript levels in the sADK lines relative to the WT, consistent with the RNA blot results. Transcripts of RNA helicase were amplified as an internal control.
    Sequencing Total Leaf Rna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Illumina Inc polyadenylated leaf rna library
    Analysis of ADK transcript abundance by <t>RNA</t> blotting and reverse transcription <t>(RT)-PCR.</t> A, Total RNA from leaves and stems of the indicated lines was probed with the full-length ADK1 cDNA. The silenced lines contain reduced ADK transcript levels in leaves and a less moderate decrease in the apparently normal secondary shoots. B, RT-PCR analysis of cDNA prepared from RNA of the indicates a reduction in ADK1 and ADK2 transcript levels in the sADK lines relative to the WT, consistent with the RNA blot results. Transcripts of RNA helicase were amplified as an internal control.
    Polyadenylated Leaf Rna Library, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega leaf total rna
    Analysis of ADK transcript abundance by <t>RNA</t> blotting and reverse transcription <t>(RT)-PCR.</t> A, Total RNA from leaves and stems of the indicated lines was probed with the full-length ADK1 cDNA. The silenced lines contain reduced ADK transcript levels in leaves and a less moderate decrease in the apparently normal secondary shoots. B, RT-PCR analysis of cDNA prepared from RNA of the indicates a reduction in ADK1 and ADK2 transcript levels in the sADK lines relative to the WT, consistent with the RNA blot results. Transcripts of RNA helicase were amplified as an internal control.
    Leaf Total Rna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Vernalis rna extraction rt pcr matk splicing activity leaf tissue
    Mature trnK (UUU) product from three orchid species. <t>MatK</t> activity in orchids was determined by examining whether the trnK (UUU) intron was effectively removed from precursor transcript. Total <t>RNA</t> was isolated from three orchid species, Phaius tancarvilleae , Spiranthes cernua and S. vernalis and subsequently converted to cDNA by first strand synthesis using an oligo dT (20) primer. Mature trnK (UUU) product resulting from removal of intervening group IIA intron and ligation of surrounding trnK exons resulted in a <t>RT-PCR</t> product range from 50 to 61 bp depending on species based on binding position of primers NITtrnkRev and trnK 3’end primer (this study). Precursor RNA still containing intron would result in a band of ~2800 bp based on these same primers. (a) Annotation of primers to the trnK/ matK gene region. (b) RT-PCR products using primers specific to the trnK (UUU) exons resolved on 1.7 % agarose gels. Lanes: L1, 1 Kb ladder (New England Biolabs), L2, BenchTop PCR markers (Promega), 1–3 represent products from RT-PCR of three biological replicates for each orchid species examined, neg is the negative control. Sizes of relevant DNA standards based on expected or identified PCR products are shown. Arrows indicate mature trnK (UUU) product. Primer dimer due to secondary structure of primers used in PCR is evident as a faint disperse band below the 50 bp standard in negative controls
    Rna Extraction Rt Pcr Matk Splicing Activity Leaf Tissue, supplied by Vernalis, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    NMTase mRNA expression in Limonium sp. Total RNA (10 μg) per lane was used and probed with clone 23 DNA probe (top) or a L . latifolium actin probe (middle). The bottom panel shows an ethidium bromide-stained gel as a loading control. Mature leaf RNA was from L . latifolium (L) and L . sinuatum (S). The arrow points to 1.4-kb size.

    Journal: Plant Physiology

    Article Title: ?-Alanine N-Methyltransferase of Limonium latifolium. cDNA Cloning and Functional Expression of a NovelN-Methyltransferase Implicated in the Synthesis of the Osmoprotectant ?-Alanine Betaine 1

    doi: 10.1104/pp.103.020453

    Figure Lengend Snippet: NMTase mRNA expression in Limonium sp. Total RNA (10 μg) per lane was used and probed with clone 23 DNA probe (top) or a L . latifolium actin probe (middle). The bottom panel shows an ethidium bromide-stained gel as a loading control. Mature leaf RNA was from L . latifolium (L) and L . sinuatum (S). The arrow points to 1.4-kb size.

    Article Snippet: Poly(A+ ) RNA was isolated from leaf total RNA using Oligo(dT)-cellulose (GenElute-mRNA miniprep kit, Sigma-Aldrich).

    Techniques: Expressing, Staining

    A, β-Ala NMTase mRNA expression in various tissues of L . latifolium . Total RNA from young leaves (Y), mature leaves (M), old leaves (O), flower (F), floral stem (S), and root (R) was separated and probed with either NMTase cDNA (clone 23) probe (top) or actin cDNA probe (middle). The bottom panel shows an ethidium bromide-stained RNA as a loading control. B, β-Ala NMTase mRNA expression in excised leaves of L . latifolium as modulated by external treatments. L, Exposure to light; D, dark; K, kinetin; I, IBA; G, GA 3 ; and N, NaCl. The middle panel shows corresponding RNA blot probed with L . latifolium actin cDNA. The bottom panel shows an ethidium bromide-stained RNA as a loading control.

    Journal: Plant Physiology

    Article Title: ?-Alanine N-Methyltransferase of Limonium latifolium. cDNA Cloning and Functional Expression of a NovelN-Methyltransferase Implicated in the Synthesis of the Osmoprotectant ?-Alanine Betaine 1

    doi: 10.1104/pp.103.020453

    Figure Lengend Snippet: A, β-Ala NMTase mRNA expression in various tissues of L . latifolium . Total RNA from young leaves (Y), mature leaves (M), old leaves (O), flower (F), floral stem (S), and root (R) was separated and probed with either NMTase cDNA (clone 23) probe (top) or actin cDNA probe (middle). The bottom panel shows an ethidium bromide-stained RNA as a loading control. B, β-Ala NMTase mRNA expression in excised leaves of L . latifolium as modulated by external treatments. L, Exposure to light; D, dark; K, kinetin; I, IBA; G, GA 3 ; and N, NaCl. The middle panel shows corresponding RNA blot probed with L . latifolium actin cDNA. The bottom panel shows an ethidium bromide-stained RNA as a loading control.

    Article Snippet: Poly(A+ ) RNA was isolated from leaf total RNA using Oligo(dT)-cellulose (GenElute-mRNA miniprep kit, Sigma-Aldrich).

    Techniques: Expressing, Staining, Northern blot

    Analysis of ADK transcript abundance by RNA blotting and reverse transcription (RT)-PCR. A, Total RNA from leaves and stems of the indicated lines was probed with the full-length ADK1 cDNA. The silenced lines contain reduced ADK transcript levels in leaves and a less moderate decrease in the apparently normal secondary shoots. B, RT-PCR analysis of cDNA prepared from RNA of the indicates a reduction in ADK1 and ADK2 transcript levels in the sADK lines relative to the WT, consistent with the RNA blot results. Transcripts of RNA helicase were amplified as an internal control.

    Journal: Plant Physiology

    Article Title: Adenosine Kinase Deficiency Is Associated with Developmental Abnormalities and Reduced Transmethylation 1

    doi: 10.1104/pp.010880

    Figure Lengend Snippet: Analysis of ADK transcript abundance by RNA blotting and reverse transcription (RT)-PCR. A, Total RNA from leaves and stems of the indicated lines was probed with the full-length ADK1 cDNA. The silenced lines contain reduced ADK transcript levels in leaves and a less moderate decrease in the apparently normal secondary shoots. B, RT-PCR analysis of cDNA prepared from RNA of the indicates a reduction in ADK1 and ADK2 transcript levels in the sADK lines relative to the WT, consistent with the RNA blot results. Transcripts of RNA helicase were amplified as an internal control.

    Article Snippet: The same primers were used for RT-PCR analysis of cDNA obtained from 2 μg of total leaf RNA (MBI Fermentas, Burlington, ON).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Northern blot, Amplification

    Mature trnK (UUU) product from three orchid species. MatK activity in orchids was determined by examining whether the trnK (UUU) intron was effectively removed from precursor transcript. Total RNA was isolated from three orchid species, Phaius tancarvilleae , Spiranthes cernua and S. vernalis and subsequently converted to cDNA by first strand synthesis using an oligo dT (20) primer. Mature trnK (UUU) product resulting from removal of intervening group IIA intron and ligation of surrounding trnK exons resulted in a RT-PCR product range from 50 to 61 bp depending on species based on binding position of primers NITtrnkRev and trnK 3’end primer (this study). Precursor RNA still containing intron would result in a band of ~2800 bp based on these same primers. (a) Annotation of primers to the trnK/ matK gene region. (b) RT-PCR products using primers specific to the trnK (UUU) exons resolved on 1.7 % agarose gels. Lanes: L1, 1 Kb ladder (New England Biolabs), L2, BenchTop PCR markers (Promega), 1–3 represent products from RT-PCR of three biological replicates for each orchid species examined, neg is the negative control. Sizes of relevant DNA standards based on expected or identified PCR products are shown. Arrows indicate mature trnK (UUU) product. Primer dimer due to secondary structure of primers used in PCR is evident as a faint disperse band below the 50 bp standard in negative controls

    Journal: BMC Evolutionary Biology

    Article Title: Alternative translation initiation codons for the plastid maturase MatK: unraveling the pseudogene misconception in the Orchidaceae

    doi: 10.1186/s12862-015-0491-1

    Figure Lengend Snippet: Mature trnK (UUU) product from three orchid species. MatK activity in orchids was determined by examining whether the trnK (UUU) intron was effectively removed from precursor transcript. Total RNA was isolated from three orchid species, Phaius tancarvilleae , Spiranthes cernua and S. vernalis and subsequently converted to cDNA by first strand synthesis using an oligo dT (20) primer. Mature trnK (UUU) product resulting from removal of intervening group IIA intron and ligation of surrounding trnK exons resulted in a RT-PCR product range from 50 to 61 bp depending on species based on binding position of primers NITtrnkRev and trnK 3’end primer (this study). Precursor RNA still containing intron would result in a band of ~2800 bp based on these same primers. (a) Annotation of primers to the trnK/ matK gene region. (b) RT-PCR products using primers specific to the trnK (UUU) exons resolved on 1.7 % agarose gels. Lanes: L1, 1 Kb ladder (New England Biolabs), L2, BenchTop PCR markers (Promega), 1–3 represent products from RT-PCR of three biological replicates for each orchid species examined, neg is the negative control. Sizes of relevant DNA standards based on expected or identified PCR products are shown. Arrows indicate mature trnK (UUU) product. Primer dimer due to secondary structure of primers used in PCR is evident as a faint disperse band below the 50 bp standard in negative controls

    Article Snippet: RNA Extraction/ RT-PCR/ MatK Splicing Activity Leaf tissue was harvested from three biological replicates each of S. vernalis, S. cernua and P. tancarvilleae .

    Techniques: Activity Assay, Isolation, Ligation, Reverse Transcription Polymerase Chain Reaction, Binding Assay, Polymerase Chain Reaction, Negative Control