Journal: Molecular & Cellular Proteomics : MCP
Article Title: Diverse Peptide Hormones Affecting Root Growth Identified in the Medicago truncatula Secreted Peptidome *
Figure Lengend Snippet: MS/MS spectra of the extended MtCEP1 peptides. A , The D1 extended peptide consisting of 41 amino acids (D1–41). Nano-LC-MS/MS spectrum at 26.96 min ( m / z 867.61340) showed a signature ion fragment at y6 indicating hydroxylation at P36. B , Identification of the D1 extended peptide consisting of 23 amino acids (D1–23). Nano-MS/MS spectrum at 13.55 min ( m / z 799.69806) showed signature ion fragments peaks at y6, y10, and y13 indicating hydroxylation at P11, 14, 18. C , Identification of the D2 extended peptide consisting of 20 amino acids (D2–20). MS/MS spectrum at 15.73 min ( m / z 1063.48047) showed signature ion peak y6 indicating hydroxylation at P15.
Article Snippet: The samples derived from root cultures containing vector control or 35S : MtCEP1 or xylem sap were subjected to nano-LC-MS/MS (Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer, Thermo Fisher Scientific, San Jose, CA) using 7 μl of sample per technical repeat ( ).
Techniques: Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy