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    Close your uncertainty gap with HRAM high resolution accurate mass spectrometry Today s forensic toxicology and clinical research laboratory demands more application versatility data accuracy and value from the LC
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    99
    Thermo Fisher liquid chromatography mass spectrometry
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
    Liquid Chromatography Mass Spectrometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher optima grade liquid chromatography mass spectrometry lc ms water
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    Thermo Fisher liquid chromatography mass spectrometry lc ms analysis
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    Thermo Fisher orbitrap liquid chromatography mass spectrometry lc ms
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    Thermo Fisher liquid chromatography mass spectrometry lc ms apparatus apparatus lcq advantage mass spectrometer
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
    Liquid Chromatography Mass Spectrometry Lc Ms Apparatus Apparatus Lcq Advantage Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher acetonitrile liquid chromatography mass spectrometry lc ms grade
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    Thermo Fisher q exactive high resolution liquid chromatography mass spectrometry lc ms system
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
    Q Exactive High Resolution Liquid Chromatography Mass Spectrometry Lc Ms System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher liquid chromatograph mass spectrometry lc ms system
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
    Liquid Chromatograph Mass Spectrometry Lc Ms System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tsq quantum access triple quadrupole liquid chromatography mass spectrometry lc ms system
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
    Tsq Quantum Access Triple Quadrupole Liquid Chromatography Mass Spectrometry Lc Ms System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher api 4000 liquid chromatography mass spectrometry lc ms system
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
    Api 4000 Liquid Chromatography Mass Spectrometry Lc Ms System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher liquid chromatography tandem mass spectrometry lc ms
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
    Liquid Chromatography Tandem Mass Spectrometry Lc Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher api 3000 liquid chromatography mass spectrometry lc ms
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
    Api 3000 Liquid Chromatography Mass Spectrometry Lc Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher liquid chromatography mass spectrometry lc ms grade methanol
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
    Liquid Chromatography Mass Spectrometry Lc Ms Grade Methanol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher finnigan surveyor msq plus liquid chromatography mass spectrometry lc ms system
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
    Finnigan Surveyor Msq Plus Liquid Chromatography Mass Spectrometry Lc Ms System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher liquid chromatography mass spectrometry surveyor msq plus
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
    Liquid Chromatography Mass Spectrometry Surveyor Msq Plus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher liquid chromatography mass spectrometry system
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    Thermo Fisher nanoflow liquid chromatography mass spectrometry
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    Thermo Fisher scientific liquid chromatography mass spectrometry mass spectrometry
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    Thermo Fisher ltq orbitrap velos liquid chromatography mass spectrometry lc ms
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
    Ltq Orbitrap Velos Liquid Chromatography Mass Spectrometry Lc Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high performance liquid chromatography mass spectrometry
    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to <t>liquid</t> <t>chromatography</t> coupled with tandem <t>mass</t> <t>spectrometry,</t> as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.
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    Thermo Fisher nano liquid chromatography mass spectrometry
    MS/MS spectra of the extended <t>MtCEP1</t> peptides. A , The D1 extended peptide consisting of 41 amino acids (D1–41). <t>Nano-LC-MS/MS</t> spectrum at 26.96 min ( m / z 867.61340) showed a signature ion fragment at y6 indicating hydroxylation at P36. B , Identification of the D1 extended peptide consisting of 23 amino acids (D1–23). Nano-MS/MS spectrum at 13.55 min ( m / z 799.69806) showed signature ion fragments peaks at y6, y10, and y13 indicating hydroxylation at P11, 14, 18. C , Identification of the D2 extended peptide consisting of 20 amino acids (D2–20). MS/MS spectrum at 15.73 min ( m / z 1063.48047) showed signature ion peak y6 indicating hydroxylation at P15.
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    Image Search Results


    E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to liquid chromatography coupled with tandem mass spectrometry, as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Short‐Term E‐Cigarette Exposure Increases the Risk of Thrombogenesis and Enhances Platelet Function in Mice

    doi: 10.1161/JAHA.118.009264

    Figure Lengend Snippet: E‐cigarette exposure results in systemic delivery of nicotine measured as the marker cotinine. Plasma from clean air or e‐cigarette–exposed mice was subjected to liquid chromatography coupled with tandem mass spectrometry, as described in the “Methods” section, and the levels of cotinine were determined. Briefly, plasma samples were spiked with 20 ng/mL cotinine‐ d 3 (internal standard) before organic solvent extraction of nicotine and its major metabolite, cotinine. Metabolites were separated by reversed‐phase chromatography/ reversed‐phase chromatography and detected and quantified by liquid chromatography–tandem mass spectrometry (positive‐ion mode) using selected reaction monitoring. Peaks corresponding to major product ions for cotinine‐ d 3 ( m/z 146.20) and cotinine ( m/z 80.45) were integrated for quantification as indicated. Chromatograms are representative of biological duplicates and technical triplicates. Measurements were performed on several individual animals from at least 3 different exposures.

    Article Snippet: All solvents used above were liquid chromatography–mass spectrometry grade from Thermo Fisher Scientific.

    Techniques: Marker, Mouse Assay, Liquid Chromatography, Mass Spectrometry, Reversed-phase Chromatography

    MS/MS spectra of the extended MtCEP1 peptides. A , The D1 extended peptide consisting of 41 amino acids (D1–41). Nano-LC-MS/MS spectrum at 26.96 min ( m / z 867.61340) showed a signature ion fragment at y6 indicating hydroxylation at P36. B , Identification of the D1 extended peptide consisting of 23 amino acids (D1–23). Nano-MS/MS spectrum at 13.55 min ( m / z 799.69806) showed signature ion fragments peaks at y6, y10, and y13 indicating hydroxylation at P11, 14, 18. C , Identification of the D2 extended peptide consisting of 20 amino acids (D2–20). MS/MS spectrum at 15.73 min ( m / z 1063.48047) showed signature ion peak y6 indicating hydroxylation at P15.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Diverse Peptide Hormones Affecting Root Growth Identified in the Medicago truncatula Secreted Peptidome *

    doi: 10.1074/mcp.RA117.000168

    Figure Lengend Snippet: MS/MS spectra of the extended MtCEP1 peptides. A , The D1 extended peptide consisting of 41 amino acids (D1–41). Nano-LC-MS/MS spectrum at 26.96 min ( m / z 867.61340) showed a signature ion fragment at y6 indicating hydroxylation at P36. B , Identification of the D1 extended peptide consisting of 23 amino acids (D1–23). Nano-MS/MS spectrum at 13.55 min ( m / z 799.69806) showed signature ion fragments peaks at y6, y10, and y13 indicating hydroxylation at P11, 14, 18. C , Identification of the D2 extended peptide consisting of 20 amino acids (D2–20). MS/MS spectrum at 15.73 min ( m / z 1063.48047) showed signature ion peak y6 indicating hydroxylation at P15.

    Article Snippet: The samples derived from root cultures containing vector control or 35S : MtCEP1 or xylem sap were subjected to nano-LC-MS/MS (Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer, Thermo Fisher Scientific, San Jose, CA) using 7 μl of sample per technical repeat ( ).

    Techniques: Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy