lc-ms : liquid chromatography-mass Search Results


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    Millipore liquid chromatography tandem mass spectrometry
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies liquid chromatography tandem mass spectrometry
    Wld S downregulates UCP2 expression and upregulates ATP levels through SIRT1. A : Wld S colocalized with SIRT1 in MIN6 cells. The stable cell lines expressing EGFP-Wld S were transiently transfected with pCMV-myc-SIRT1 and stained with anti-Myc antibody and DAPI. Scale bar, 5 μm. B : Wld S was coimmunoprecipitated with SIRT1 from the pancreatic lysates of Wld S mice. C : Wld S and its enzyme-dead mutant Wld S -H112A coimmunoprecipitated with SIRT1. The MIN6 cells stably expressing EGFP, EGFP-Wld S , or EGFP-Wld S -H112A were used for immunoprecipitation. UCP2 protein levels were also detected by Western blot. D and E : <t>Liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry</t> analysis of NMN, NADP, NADPH, NAD, NADH, NA, and NAM extracted from pancreas of 9-week-old wild-type (WT) ( D ) or Wld S mice ( E ). *With significant difference. F : Quantification of the small molecules corresponding to D and E showed that NAD and NMN levels were upregulated in the pancreas of Wld S mice ( n = 4 for each genotype). G : Wld S repressed UCP2 promoter activity like SIRT1. UCP2 promoter activity was measured by luciferase assay in 293T cells transfected with pGL3-UCP2-Promoter and the indicated plasmids. H : Wld S downregulated UCP2 mRNA levels dependent on its enzyme activity. UCP2 mRNA level was measured by real-time PCR with MIN6 cell lines stably expressing EGFP, EGFP-Wld S , and EGFP-Wld S -H112A. I : Wld S downregulated UCP2 mRNA levels via SIRT1. UCP2 mRNA levels were determined by real-time PCR using islets isolated from mice with the indicated genotype ( n = 4 for each genotype). J : Wld S downregulated UCP2 protein levels via SIRT1. The protein levels in brown fat tissue with the indicated genotype were detected with SIRT1, Wld S , UCP2, and tubulin antibodies ( n = 3 for each genotype). K : Quantification of the UCP2 protein levels corresponding to J . L : Wld S increased ATP levels in primary cultured islets at the indicated glucose concentration ( n = 3). M : Wld S upregulated ATP level in islets via SIRT1. ATP levels were measured in islets with indicated genotypes at 2 mmol/L or 20 mmol/L glucose ( n = 3). * P
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 693 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 693 article reviews
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    93
    Waters Corporation liquid chromatography tandem mass spectrometry
    Exhaled breath (EB) samples tested by reversed‐phase <t>liquid</t> <t>chromatography/tandem</t> <t>mass</t> <t>spectrometry</t> for 12 model compounds including anabolic agents, hormone and metabolic modulators, stimulants, and beta‐blockers: (A) blank EB specimen containing only the internal standards D 3 ‐testosterone, D 3 ‐meldonium, ( S )‐2‐aminooctane, and D 7 ‐propranolol. Y‐axes are normalized to the abundance of the corresponding spiked specimen shown under (B), which illustrates the results of an EB sample fortified with 500 pg of each target analyte plus ISTDs
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-09
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    92
    Agilent technologies liquid chromatography mass spectrometry
    Modulatory effect of XanMax ® 2002 oil on Lutein and Zeaxanthin levels in serum and macula portion of the retina from rats. Rats were treated with XanMax® 2002 oil for 42 days and lutein and zeaxanthin values in serum (a and b respectively), and macular homogenates (c and d respectively) were determined by <t>liquid</t> <t>chromatography-mass</t> <t>spectrometry</t> and expressed as ppb concentration. Values are expressed as mean ± standard deviation for each group; *** P
    Liquid Chromatography Mass Spectrometry, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Waters Corporation liquid chromatography mass spectrometry
    <t>Ultra-performance</t> <t>liquid</t> <t>chromatography–mass</t> <t>spectrometry</t> combined with electrospray ionization (UPLC–ESI–MS/MS) total ion current chromatograms. ( A ) The blank human liver microsomes sample. ( B ) The metabolism sample of imperatorin, with metabolites of M1 and M2. ( C ) The metabolism sample of isoimperatorin, with metabolites of M3 and M4.
    Liquid Chromatography Mass Spectrometry, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 381 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Shimadzu Corporation liquid chromatography tandem mass spectrometry
    BMS-754807 has limited drug delivery to the tumor in the brainstem. Nestin-Tv-a;p53 fl/fl mice were injected with DF1 cells expressing RCAS-PDGF-B, RCAS-Cre, and RCAS-H3.3-K27M. Upon appearance of symptoms, mice were treated with either BMS-754807 or vehicle for 3 doses, and sacrificed at 4 hours after the last dose. Tissue concentrations of BMS-754807 were determined by <t>liquid</t> <t>chromatography</t> <t>tandem-mass</t> <t>spectrometry</t> (LC/MS/MS). There was a significant difference between BMS-754807 treated tumor lysates compared to vehicle treated tumor lysates (p = 0.0357 by Mann-Whitney) and between BMS-754807 treated normal brain lysates as compared to vehicle treated normal brain lysates (p = 0.0357 by Mann-Whitney).
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 93/100, based on 306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-09
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    93
    Shimadzu Corporation liquid chromatography mass spectrometry
    BMS-754807 has limited drug delivery to the tumor in the brainstem. Nestin-Tv-a;p53 fl/fl mice were injected with DF1 cells expressing RCAS-PDGF-B, RCAS-Cre, and RCAS-H3.3-K27M. Upon appearance of symptoms, mice were treated with either BMS-754807 or vehicle for 3 doses, and sacrificed at 4 hours after the last dose. Tissue concentrations of BMS-754807 were determined by <t>liquid</t> <t>chromatography</t> <t>tandem-mass</t> <t>spectrometry</t> (LC/MS/MS). There was a significant difference between BMS-754807 treated tumor lysates compared to vehicle treated tumor lysates (p = 0.0357 by Mann-Whitney) and between BMS-754807 treated normal brain lysates as compared to vehicle treated normal brain lysates (p = 0.0357 by Mann-Whitney).
    Liquid Chromatography Mass Spectrometry, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 93/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography mass spectrometry/product/Shimadzu Corporation
    Average 93 stars, based on 265 article reviews
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    liquid chromatography mass spectrometry - by Bioz Stars, 2020-09
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    94
    Thermo Fisher liquid chromatography tandem mass spectrometry
    Absolute levels of tryptophan in urine samples. Tryptophan levels were measured from SLC22A2 reference ( n = 9), 808GT ( n = 6), and 808TT ( n = 6) carriers using selected reaction monitoring with <t>liquid</t> <t>chromatography-coupled</t> <t>tandem</t> <t>mass</t> <t>spectrometry.</t> The boundary of the box closest to zero indicates the 25 th percentile, a line within the box marks the median, and the boundary of the box farthest from zero indicates the 75 th percentile.
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-09
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    94
    Thermo Fisher liquid chromatography mass spectrometry
    Absolute levels of tryptophan in urine samples. Tryptophan levels were measured from SLC22A2 reference ( n = 9), 808GT ( n = 6), and 808TT ( n = 6) carriers using selected reaction monitoring with <t>liquid</t> <t>chromatography-coupled</t> <t>tandem</t> <t>mass</t> <t>spectrometry.</t> The boundary of the box closest to zero indicates the 25 th percentile, a line within the box marks the median, and the boundary of the box farthest from zero indicates the 75 th percentile.
    Liquid Chromatography Mass Spectrometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 816 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies liquid chromatography electrospray ionization mass spectrometry
    Representative of liquid <t>chromatography-electrospray</t> ionization mass spectrometry (LC-ESI-MS) selected ion chromatogram ( m / z = 353) of vTP+ vessels incubated in HEPES buffer with and without angiotensin II [0.1 μM; aorta ( A ) or mesentery artery
    Liquid Chromatography Electrospray Ionization Mass Spectrometry, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography electrospray ionization mass spectrometry/product/Agilent technologies
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    92
    Quest Diagnostics liquid chromatography tandem mass spectrometry
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Liquid Chromatography Tandem Mass Spectrometry, supplied by Quest Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography tandem mass spectrometry/product/Quest Diagnostics
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    liquid chromatography tandem mass spectrometry - by Bioz Stars, 2020-09
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    91
    Waters Corporation nano liquid chromatography tandem mass spectrometry
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Nano Liquid Chromatography Tandem Mass Spectrometry, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano liquid chromatography tandem mass spectrometry/product/Waters Corporation
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    91
    Metabolon Inc ultra high performance liquid chromatography tandem mass spectrometry
    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - <t>liquid</t> <t>chromatography-tandem</t> <t>mass</t> <t>spectrometry,</t> HPLC - high performance liquid chromatography
    Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry, supplied by Metabolon Inc, used in various techniques. Bioz Stars score: 91/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra high performance liquid chromatography tandem mass spectrometry/product/Metabolon Inc
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    Image Search Results


    Wld S downregulates UCP2 expression and upregulates ATP levels through SIRT1. A : Wld S colocalized with SIRT1 in MIN6 cells. The stable cell lines expressing EGFP-Wld S were transiently transfected with pCMV-myc-SIRT1 and stained with anti-Myc antibody and DAPI. Scale bar, 5 μm. B : Wld S was coimmunoprecipitated with SIRT1 from the pancreatic lysates of Wld S mice. C : Wld S and its enzyme-dead mutant Wld S -H112A coimmunoprecipitated with SIRT1. The MIN6 cells stably expressing EGFP, EGFP-Wld S , or EGFP-Wld S -H112A were used for immunoprecipitation. UCP2 protein levels were also detected by Western blot. D and E : Liquid chromatography-tandem mass spectrometry analysis of NMN, NADP, NADPH, NAD, NADH, NA, and NAM extracted from pancreas of 9-week-old wild-type (WT) ( D ) or Wld S mice ( E ). *With significant difference. F : Quantification of the small molecules corresponding to D and E showed that NAD and NMN levels were upregulated in the pancreas of Wld S mice ( n = 4 for each genotype). G : Wld S repressed UCP2 promoter activity like SIRT1. UCP2 promoter activity was measured by luciferase assay in 293T cells transfected with pGL3-UCP2-Promoter and the indicated plasmids. H : Wld S downregulated UCP2 mRNA levels dependent on its enzyme activity. UCP2 mRNA level was measured by real-time PCR with MIN6 cell lines stably expressing EGFP, EGFP-Wld S , and EGFP-Wld S -H112A. I : Wld S downregulated UCP2 mRNA levels via SIRT1. UCP2 mRNA levels were determined by real-time PCR using islets isolated from mice with the indicated genotype ( n = 4 for each genotype). J : Wld S downregulated UCP2 protein levels via SIRT1. The protein levels in brown fat tissue with the indicated genotype were detected with SIRT1, Wld S , UCP2, and tubulin antibodies ( n = 3 for each genotype). K : Quantification of the UCP2 protein levels corresponding to J . L : Wld S increased ATP levels in primary cultured islets at the indicated glucose concentration ( n = 3). M : Wld S upregulated ATP level in islets via SIRT1. ATP levels were measured in islets with indicated genotypes at 2 mmol/L or 20 mmol/L glucose ( n = 3). * P

    Journal: Diabetes

    Article Title: WldS Enhances Insulin Transcription and Secretion via a SIRT1-Dependent Pathway and Improves Glucose Homeostasis

    doi: 10.2337/db11-0232

    Figure Lengend Snippet: Wld S downregulates UCP2 expression and upregulates ATP levels through SIRT1. A : Wld S colocalized with SIRT1 in MIN6 cells. The stable cell lines expressing EGFP-Wld S were transiently transfected with pCMV-myc-SIRT1 and stained with anti-Myc antibody and DAPI. Scale bar, 5 μm. B : Wld S was coimmunoprecipitated with SIRT1 from the pancreatic lysates of Wld S mice. C : Wld S and its enzyme-dead mutant Wld S -H112A coimmunoprecipitated with SIRT1. The MIN6 cells stably expressing EGFP, EGFP-Wld S , or EGFP-Wld S -H112A were used for immunoprecipitation. UCP2 protein levels were also detected by Western blot. D and E : Liquid chromatography-tandem mass spectrometry analysis of NMN, NADP, NADPH, NAD, NADH, NA, and NAM extracted from pancreas of 9-week-old wild-type (WT) ( D ) or Wld S mice ( E ). *With significant difference. F : Quantification of the small molecules corresponding to D and E showed that NAD and NMN levels were upregulated in the pancreas of Wld S mice ( n = 4 for each genotype). G : Wld S repressed UCP2 promoter activity like SIRT1. UCP2 promoter activity was measured by luciferase assay in 293T cells transfected with pGL3-UCP2-Promoter and the indicated plasmids. H : Wld S downregulated UCP2 mRNA levels dependent on its enzyme activity. UCP2 mRNA level was measured by real-time PCR with MIN6 cell lines stably expressing EGFP, EGFP-Wld S , and EGFP-Wld S -H112A. I : Wld S downregulated UCP2 mRNA levels via SIRT1. UCP2 mRNA levels were determined by real-time PCR using islets isolated from mice with the indicated genotype ( n = 4 for each genotype). J : Wld S downregulated UCP2 protein levels via SIRT1. The protein levels in brown fat tissue with the indicated genotype were detected with SIRT1, Wld S , UCP2, and tubulin antibodies ( n = 3 for each genotype). K : Quantification of the UCP2 protein levels corresponding to J . L : Wld S increased ATP levels in primary cultured islets at the indicated glucose concentration ( n = 3). M : Wld S upregulated ATP level in islets via SIRT1. ATP levels were measured in islets with indicated genotypes at 2 mmol/L or 20 mmol/L glucose ( n = 3). * P

    Article Snippet: The samples were then analyzed using a liquid chromatography-tandem mass spectrometry with an Agilent 1200 series high-performance liquid chromatography system and a 4000 Q Trap tandem mass spectrometer (Applied Biosystems).

    Techniques: Expressing, Stable Transfection, Transfection, Staining, Mouse Assay, Mutagenesis, Immunoprecipitation, Western Blot, Liquid Chromatography, Mass Spectrometry, Activity Assay, Luciferase, Real-time Polymerase Chain Reaction, Isolation, Cell Culture, Concentration Assay

    Exhaled breath (EB) samples tested by reversed‐phase liquid chromatography/tandem mass spectrometry for 12 model compounds including anabolic agents, hormone and metabolic modulators, stimulants, and beta‐blockers: (A) blank EB specimen containing only the internal standards D 3 ‐testosterone, D 3 ‐meldonium, ( S )‐2‐aminooctane, and D 7 ‐propranolol. Y‐axes are normalized to the abundance of the corresponding spiked specimen shown under (B), which illustrates the results of an EB sample fortified with 500 pg of each target analyte plus ISTDs

    Journal: Rapid Communications in Mass Spectrometry

    Article Title: Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath. Expanding analytical options in sports drug testing: Mass spectrometric detection of prohibited substances in exhaled breath

    doi: 10.1002/rcm.7903

    Figure Lengend Snippet: Exhaled breath (EB) samples tested by reversed‐phase liquid chromatography/tandem mass spectrometry for 12 model compounds including anabolic agents, hormone and metabolic modulators, stimulants, and beta‐blockers: (A) blank EB specimen containing only the internal standards D 3 ‐testosterone, D 3 ‐meldonium, ( S )‐2‐aminooctane, and D 7 ‐propranolol. Y‐axes are normalized to the abundance of the corresponding spiked specimen shown under (B), which illustrates the results of an EB sample fortified with 500 pg of each target analyte plus ISTDs

    Article Snippet: 2.2 Liquid chromatography/tandem mass spectrometry (LC/MS/MS) All analyses were conducted using a Aquity I‐Class ultra‐performance liquid chromatography (UPLC) system (Waters, Eschborn, Germany) interfaced via unispray (US) ionization to a Xevo TQ‐XS tandem mass spectrometer (Waters).

    Techniques: Liquid Chromatography, Mass Spectrometry

    Ultra-high performance liquid chromatography-tandem mass spectrometry analysis of protein-bound BMAA extracts. Chromatograms of ( a ) blank lamb’s brain extract, ( b ) a matrix matched standard solution of BMAA, ( c ) control patient’s brain extract and ( d ) alzheimer’s diseased brain extract. (MRM, multiple reaction monitoring).

    Journal: Scientific Reports

    Article Title: β-methylamino-L-alanine (BMAA) is not found in the brains of patients with confirmed Alzheimer’s disease

    doi: 10.1038/srep36363

    Figure Lengend Snippet: Ultra-high performance liquid chromatography-tandem mass spectrometry analysis of protein-bound BMAA extracts. Chromatograms of ( a ) blank lamb’s brain extract, ( b ) a matrix matched standard solution of BMAA, ( c ) control patient’s brain extract and ( d ) alzheimer’s diseased brain extract. (MRM, multiple reaction monitoring).

    Article Snippet: Ultra-performance liquid chromatography tandem mass spectrometry [UPLC-MS/MS] Analysis of the brain samples was performed using an Acquity UPLC I-Class system coupled to a Xevo TQ-S (triple quadrupole MS/MS) mass spectrometer (Waters, Ireland).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    Ultra-high performance liquid chromatography-tandem mass spectrometry analysis of free BMAA extracts. Chromatograms of ( a ) blank lamb’s brain extract, ( b ) a matrix matched standard solution of BMAA, ( c ) control patient’s brain extract and ( d ) alzheimer’s diseased brain extract. (MRM, multiple reaction monitoring).

    Journal: Scientific Reports

    Article Title: β-methylamino-L-alanine (BMAA) is not found in the brains of patients with confirmed Alzheimer’s disease

    doi: 10.1038/srep36363

    Figure Lengend Snippet: Ultra-high performance liquid chromatography-tandem mass spectrometry analysis of free BMAA extracts. Chromatograms of ( a ) blank lamb’s brain extract, ( b ) a matrix matched standard solution of BMAA, ( c ) control patient’s brain extract and ( d ) alzheimer’s diseased brain extract. (MRM, multiple reaction monitoring).

    Article Snippet: Ultra-performance liquid chromatography tandem mass spectrometry [UPLC-MS/MS] Analysis of the brain samples was performed using an Acquity UPLC I-Class system coupled to a Xevo TQ-S (triple quadrupole MS/MS) mass spectrometer (Waters, Ireland).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    Ultra-high performance liquid chromatography-tandem mass spectrometry chromatograms obtained for ( a ) BMAA-DNS, ( b ) DAB-DNS and ( c ) D 3 -BMAA-DNS solvent standards at 250 ng/ml, 250 ng/ml and 100 ng/ml, respectively. (MRM, multiple reaction monitoring).

    Journal: Scientific Reports

    Article Title: β-methylamino-L-alanine (BMAA) is not found in the brains of patients with confirmed Alzheimer’s disease

    doi: 10.1038/srep36363

    Figure Lengend Snippet: Ultra-high performance liquid chromatography-tandem mass spectrometry chromatograms obtained for ( a ) BMAA-DNS, ( b ) DAB-DNS and ( c ) D 3 -BMAA-DNS solvent standards at 250 ng/ml, 250 ng/ml and 100 ng/ml, respectively. (MRM, multiple reaction monitoring).

    Article Snippet: Ultra-performance liquid chromatography tandem mass spectrometry [UPLC-MS/MS] Analysis of the brain samples was performed using an Acquity UPLC I-Class system coupled to a Xevo TQ-S (triple quadrupole MS/MS) mass spectrometer (Waters, Ireland).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    LC-MS chromatograms (collected with ultraperformance liquid chromatography coupled to a Q-Exactive Orbitrap mass spectrometer) of a series of H. canadensis extract fractions. (A) Positive-ion electrospray mass spectrometry chromatogram of the crude extract after liquid–liquid partitioning. (B) The most active fraction (GS-4) of the crude extract shown in A after separation with flash chromatography over silica gel using a hexane–chloroform–methanol gradient (stage 1 separation). GS-4 was further fractionated using a second stage of flash chromatography over silica gel with a hexane–ethyl acetate–methanol gradient with GS-4-4 being the most active (C). GS-4-4-2 generated with stage 3 fractionation (using reversed-phase preparative HPLC with a water–acetonitrile gradient) (D). (E–H) The same H. canadensis extract and fractions analyzed using LC-MS in the negative-ion mode. Arrows represent the region of the chromatogram in which a given ion corresponding to a known constituent of H. canadensis (indicated by compound numbers) could be detected. Compounds 1 – 7 , 10 , 13 , 16 , 17 , 20 – 24 , and 28 while compounds 8 , 9 , 11 , 12 , 14 , 15 , 18 , 19 , and 25 – 27 29 is a new flavonoid that was isolated and identified based on NMR and MS data as part of this report. Red dotted arrows represent alkaloids and other compounds, while green dashed arrows represent flavonoids. In cases where a specific peak is not apparent in the chromatogram, the ion was identified based on mass spectrometric data.

    Journal: Journal of natural products

    Article Title: Biochemometrics to Identify Synergists and Additives from Botanical Medicines: A Case Study with Hydrastis canadensis (Goldenseal)

    doi: 10.1021/acs.jnatprod.7b00654

    Figure Lengend Snippet: LC-MS chromatograms (collected with ultraperformance liquid chromatography coupled to a Q-Exactive Orbitrap mass spectrometer) of a series of H. canadensis extract fractions. (A) Positive-ion electrospray mass spectrometry chromatogram of the crude extract after liquid–liquid partitioning. (B) The most active fraction (GS-4) of the crude extract shown in A after separation with flash chromatography over silica gel using a hexane–chloroform–methanol gradient (stage 1 separation). GS-4 was further fractionated using a second stage of flash chromatography over silica gel with a hexane–ethyl acetate–methanol gradient with GS-4-4 being the most active (C). GS-4-4-2 generated with stage 3 fractionation (using reversed-phase preparative HPLC with a water–acetonitrile gradient) (D). (E–H) The same H. canadensis extract and fractions analyzed using LC-MS in the negative-ion mode. Arrows represent the region of the chromatogram in which a given ion corresponding to a known constituent of H. canadensis (indicated by compound numbers) could be detected. Compounds 1 – 7 , 10 , 13 , 16 , 17 , 20 – 24 , and 28 while compounds 8 , 9 , 11 , 12 , 14 , 15 , 18 , 19 , and 25 – 27 29 is a new flavonoid that was isolated and identified based on NMR and MS data as part of this report. Red dotted arrows represent alkaloids and other compounds, while green dashed arrows represent flavonoids. In cases where a specific peak is not apparent in the chromatogram, the ion was identified based on mass spectrometric data.

    Article Snippet: Extracts were suspended in 1:1 MeOH–dioxane (LC/MS grade, Fisher Scientific, Hampton, NH, USA) at either 1 or 0.1 mg/mL and subjected to ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) analysis via a Waters Acquity UPLC with an Acquity UPLC column (BEH C18 , 1.7 μ m, 2.1 × 50 mm, Waters Corporation, Milford, MA, USA) and a Thermo Q-Exactive Plus orbitrap mass spectrometer with heated electrospray ionization (Thermo Fisher Scientific, Milford, MA, USA).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Liquid Chromatography, Mass Spectrometry, Chromatography, Generated, Fractionation, High Performance Liquid Chromatography, Isolation, Nuclear Magnetic Resonance

    Modulatory effect of XanMax ® 2002 oil on Lutein and Zeaxanthin levels in serum and macula portion of the retina from rats. Rats were treated with XanMax® 2002 oil for 42 days and lutein and zeaxanthin values in serum (a and b respectively), and macular homogenates (c and d respectively) were determined by liquid chromatography-mass spectrometry and expressed as ppb concentration. Values are expressed as mean ± standard deviation for each group; *** P

    Journal: Pharmacognosy Magazine

    Article Title: Modulatory Effect of Carotenoid Supplement Constituting Lutein and Zeaxanthin (10:1) on Anti-oxidant Enzymes and Macular Pigments Level in Rats

    doi: 10.4103/pm.pm_340_17

    Figure Lengend Snippet: Modulatory effect of XanMax ® 2002 oil on Lutein and Zeaxanthin levels in serum and macula portion of the retina from rats. Rats were treated with XanMax® 2002 oil for 42 days and lutein and zeaxanthin values in serum (a and b respectively), and macular homogenates (c and d respectively) were determined by liquid chromatography-mass spectrometry and expressed as ppb concentration. Values are expressed as mean ± standard deviation for each group; *** P

    Article Snippet: Equipments All the instruments, Weighing Balance (Citizen, Singapore), Microplate Reader (Biotech, USA), UV-Vis spectrophotometer (Systronics, India), Liquid chromatography-mass spectrometry (LC-MS) (Agilent 6130), and other laboratory equipment used for the experiments were calibrated and validated regularly.

    Techniques: Liquid Chromatography, Mass Spectrometry, Concentration Assay, Standard Deviation

    Liquid chromatography mass-spectrometry-based analysis of mycolic acid extracts. Abbreviation: MTB, Mycobacterium tuberculosis.

    Journal: International Journal of Nanomedicine

    Article Title: Identification of mycolic acid forms using surface-enhanced Raman scattering as a fast detection method for tuberculosis

    doi: 10.2147/IJN.S171400

    Figure Lengend Snippet: Liquid chromatography mass-spectrometry-based analysis of mycolic acid extracts. Abbreviation: MTB, Mycobacterium tuberculosis.

    Article Snippet: Liquid chromatography mass-spectrometry (LC-MS) analysis of MA LC-MS/MS analysis was conducted on an Agilent 6490 mass spectrometer coupled to an Agilent 1290 Infinity LC.

    Techniques: Liquid Chromatography, Mass Spectrometry

    Ultra-performance liquid chromatography–mass spectrometry combined with electrospray ionization (UPLC–ESI–MS/MS) total ion current chromatograms. ( A ) The blank human liver microsomes sample. ( B ) The metabolism sample of imperatorin, with metabolites of M1 and M2. ( C ) The metabolism sample of isoimperatorin, with metabolites of M3 and M4.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: A Metabolism-Based Synergy for Total Coumarin Extract of Radix Angelicae Dahuricae and Ligustrazine on Migraine Treatment in Rats

    doi: 10.3390/molecules23051004

    Figure Lengend Snippet: Ultra-performance liquid chromatography–mass spectrometry combined with electrospray ionization (UPLC–ESI–MS/MS) total ion current chromatograms. ( A ) The blank human liver microsomes sample. ( B ) The metabolism sample of imperatorin, with metabolites of M1 and M2. ( C ) The metabolism sample of isoimperatorin, with metabolites of M3 and M4.

    Article Snippet: Analysis of Imperatorin and Isoimperatorin Metabolite Using Ultra-Performance Liquid Chromatography–Mass Spectrometry (UPLC–MS/MS) The UPLC/MS/MS method was conducted using a Waters Acquity UPLC Sample Manager and a Waters Acquity UPLC Binary Solvent Manager connected to a Waters Quattro Premier XE triple-quadrupole mass spectrometer equipped with a combined ESI probe and Mass Lynx 1.4 software (Waters, MA, USA).

    Techniques: Liquid Chromatography, Mass Spectrometry

    BMS-754807 has limited drug delivery to the tumor in the brainstem. Nestin-Tv-a;p53 fl/fl mice were injected with DF1 cells expressing RCAS-PDGF-B, RCAS-Cre, and RCAS-H3.3-K27M. Upon appearance of symptoms, mice were treated with either BMS-754807 or vehicle for 3 doses, and sacrificed at 4 hours after the last dose. Tissue concentrations of BMS-754807 were determined by liquid chromatography tandem-mass spectrometry (LC/MS/MS). There was a significant difference between BMS-754807 treated tumor lysates compared to vehicle treated tumor lysates (p = 0.0357 by Mann-Whitney) and between BMS-754807 treated normal brain lysates as compared to vehicle treated normal brain lysates (p = 0.0357 by Mann-Whitney).

    Journal: PLoS ONE

    Article Title: A High-Throughput In Vitro Drug Screen in a Genetically Engineered Mouse Model of Diffuse Intrinsic Pontine Glioma Identifies BMS-754807 as a Promising Therapeutic Agent

    doi: 10.1371/journal.pone.0118926

    Figure Lengend Snippet: BMS-754807 has limited drug delivery to the tumor in the brainstem. Nestin-Tv-a;p53 fl/fl mice were injected with DF1 cells expressing RCAS-PDGF-B, RCAS-Cre, and RCAS-H3.3-K27M. Upon appearance of symptoms, mice were treated with either BMS-754807 or vehicle for 3 doses, and sacrificed at 4 hours after the last dose. Tissue concentrations of BMS-754807 were determined by liquid chromatography tandem-mass spectrometry (LC/MS/MS). There was a significant difference between BMS-754807 treated tumor lysates compared to vehicle treated tumor lysates (p = 0.0357 by Mann-Whitney) and between BMS-754807 treated normal brain lysates as compared to vehicle treated normal brain lysates (p = 0.0357 by Mann-Whitney).

    Article Snippet: Liquid chromatography tandem-mass spectrometry (LC/MS/MS) assay The analysis was performed on Shimadzu 20A series LC system coupled with Applied Biosciences/SCIEX API 4000 QTrap MS/MS.

    Techniques: Mouse Assay, Injection, Expressing, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, MANN-WHITNEY

    Absolute levels of tryptophan in urine samples. Tryptophan levels were measured from SLC22A2 reference ( n = 9), 808GT ( n = 6), and 808TT ( n = 6) carriers using selected reaction monitoring with liquid chromatography-coupled tandem mass spectrometry. The boundary of the box closest to zero indicates the 25 th percentile, a line within the box marks the median, and the boundary of the box farthest from zero indicates the 75 th percentile.

    Journal: PLoS ONE

    Article Title: Pharmacogenetics Meets Metabolomics: Discovery of Tryptophan as a New Endogenous OCT2 Substrate Related to Metformin Disposition

    doi: 10.1371/journal.pone.0036637

    Figure Lengend Snippet: Absolute levels of tryptophan in urine samples. Tryptophan levels were measured from SLC22A2 reference ( n = 9), 808GT ( n = 6), and 808TT ( n = 6) carriers using selected reaction monitoring with liquid chromatography-coupled tandem mass spectrometry. The boundary of the box closest to zero indicates the 25 th percentile, a line within the box marks the median, and the boundary of the box farthest from zero indicates the 75 th percentile.

    Article Snippet: Assay of tryptophan concentrations using liquid chromatography-tandem mass spectrometry Tryptophan concentrations in urine samples were quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS; API 3000; Applied Biosystems, Foster City, CA).

    Techniques: Liquid Chromatography, Mass Spectrometry

    Representative of liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) selected ion chromatogram ( m / z = 353) of vTP+ vessels incubated in HEPES buffer with and without angiotensin II [0.1 μM; aorta ( A ) or mesentery artery

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Role of superoxide and thromboxane receptors in acute angiotensin II-induced vasoconstriction of rabbit vessels

    doi: 10.1152/ajpheart.01135.2010

    Figure Lengend Snippet: Representative of liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) selected ion chromatogram ( m / z = 353) of vTP+ vessels incubated in HEPES buffer with and without angiotensin II [0.1 μM; aorta ( A ) or mesentery artery

    Article Snippet: Samples were analyzed by using liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS, Agilent 1100 LC/MSD, SL model) using a modification of a method previously described by Nithipatikom et al. ( ).

    Techniques: Liquid Chromatography, Mass Spectrometry, Incubation

    Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - liquid chromatography-tandem mass spectrometry, HPLC - high performance liquid chromatography

    Journal: Saudi Medical Journal

    Article Title: Assessment of low vitamin D among Saudi Arabians

    doi:

    Figure Lengend Snippet: Bland-Altman plot for CLIA-LCMS/MS. CLIA - chemiluminescence immunoassay, LC-MS/MS - liquid chromatography-tandem mass spectrometry, HPLC - high performance liquid chromatography

    Article Snippet: Liquid chromatography-tandem mass spectrometry The third method used to measure 25-OHD levels in this study was LC-MS/MS, which was carried out at the Quest Diagnostics, Lab One, Ohio, USA using an API 3000 triple-quadrupole mass spectrometer (Applied Biosystems/MDS Sciex, AB Framingham, MA, USA) following the guidelines by the US National Institute of Standards and Technology (NIST).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Chemiluminescence Immunoassay, Liquid Chromatography, High Performance Liquid Chromatography

    Bland-Altman plot for RIA-LCMS/MS. RIA - radio-immuno assay, LC-MS/MS - liquid chromatography-tandem mass spectrometry

    Journal: Saudi Medical Journal

    Article Title: Assessment of low vitamin D among Saudi Arabians

    doi:

    Figure Lengend Snippet: Bland-Altman plot for RIA-LCMS/MS. RIA - radio-immuno assay, LC-MS/MS - liquid chromatography-tandem mass spectrometry

    Article Snippet: Liquid chromatography-tandem mass spectrometry The third method used to measure 25-OHD levels in this study was LC-MS/MS, which was carried out at the Quest Diagnostics, Lab One, Ohio, USA using an API 3000 triple-quadrupole mass spectrometer (Applied Biosystems/MDS Sciex, AB Framingham, MA, USA) following the guidelines by the US National Institute of Standards and Technology (NIST).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, RIA Assay, Liquid Chromatography

    Inter assay relationship between CLIA, RIA, and LCMS/MS. CLIA - chemiluminescence immunoassay, RIA - radio-immuno assay, LC-MS/MS - liquid chromatography-tandem mass spectrometry, HPLC - high-pressure liquid chromatography

    Journal: Saudi Medical Journal

    Article Title: Assessment of low vitamin D among Saudi Arabians

    doi:

    Figure Lengend Snippet: Inter assay relationship between CLIA, RIA, and LCMS/MS. CLIA - chemiluminescence immunoassay, RIA - radio-immuno assay, LC-MS/MS - liquid chromatography-tandem mass spectrometry, HPLC - high-pressure liquid chromatography

    Article Snippet: Liquid chromatography-tandem mass spectrometry The third method used to measure 25-OHD levels in this study was LC-MS/MS, which was carried out at the Quest Diagnostics, Lab One, Ohio, USA using an API 3000 triple-quadrupole mass spectrometer (Applied Biosystems/MDS Sciex, AB Framingham, MA, USA) following the guidelines by the US National Institute of Standards and Technology (NIST).

    Techniques: Inter Assay, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Chemiluminescence Immunoassay, RIA Assay, Liquid Chromatography, High Performance Liquid Chromatography