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  • 99
    Thermo Fisher sybr select master mix
    Gene expression analysis of fatty acid-metabolizing enzymes in control and H-RasV12 expressing fibroblasts by qRT-PCR. ( A ) Gene expression analysis of desaturases ( SCD and FADS ), elongases ( ELOVL ) and acyl-coenzyme A synthetases ( ACSL ). ( B ) Gene expression analysis of phospholipases A2 ( PLA2 ). Ten ng of each cDNA were used as template. Reactions were performed in triplicate, using <t>SYBR</t> green technology <t>StepOne</t> RT-PCR machine to detect amplification. The GAPDH gene was used as endogenous control. The fold expression in H-RasV12 fibroblasts with respect to control is displayed, expressed as Relative Quantity (RQ). The analysis was repeated three times in triplicate and the mean ± S.D. is reported (* p
    Sybr Select Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16070 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr select master mix/product/Thermo Fisher
    Average 99 stars, based on 16070 article reviews
    Price from $9.99 to $1999.99
    sybr select master mix - by Bioz Stars, 2020-09
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    99
    Thermo Fisher powerup sybr green master mix
    Inhibition assays. Inhibition assays were set up as qPCR reactions using murine genomic DNA; a primer set specific to the mouse- HSD11β1 gene ( S1 Table ); and the <t>PowerUp</t> <t>SYBR</t> Green Master Mix. The reaction mixture was spiked with water (as a control) or extracted RNA or DNA from various kits. Shown are the Cq values for the control vs. reactions spiked with RNA (A) and DNA (B), in duplicate, with error bars representing standard deviations. (C) Fragment distribution of amplified RNA and DNA samples from select kits with corresponding positive controls (fresh PC-3 cells).
    Powerup Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9625 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/powerup sybr green master mix/product/Thermo Fisher
    Average 99 stars, based on 9625 article reviews
    Price from $9.99 to $1999.99
    powerup sybr green master mix - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad iscript cdna synthesis kit
    Inhibition assays. Inhibition assays were set up as qPCR reactions using murine genomic DNA; a primer set specific to the mouse- HSD11β1 gene ( S1 Table ); and the <t>PowerUp</t> <t>SYBR</t> Green Master Mix. The reaction mixture was spiked with water (as a control) or extracted RNA or DNA from various kits. Shown are the Cq values for the control vs. reactions spiked with RNA (A) and DNA (B), in duplicate, with error bars representing standard deviations. (C) Fragment distribution of amplified RNA and DNA samples from select kits with corresponding positive controls (fresh PC-3 cells).
    Iscript Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 95548 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iscript cdna synthesis kit/product/Bio-Rad
    Average 99 stars, based on 95548 article reviews
    Price from $9.99 to $1999.99
    iscript cdna synthesis kit - by Bioz Stars, 2020-09
    99/100 stars
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    Image Search Results


    Gene expression analysis of fatty acid-metabolizing enzymes in control and H-RasV12 expressing fibroblasts by qRT-PCR. ( A ) Gene expression analysis of desaturases ( SCD and FADS ), elongases ( ELOVL ) and acyl-coenzyme A synthetases ( ACSL ). ( B ) Gene expression analysis of phospholipases A2 ( PLA2 ). Ten ng of each cDNA were used as template. Reactions were performed in triplicate, using SYBR green technology StepOne RT-PCR machine to detect amplification. The GAPDH gene was used as endogenous control. The fold expression in H-RasV12 fibroblasts with respect to control is displayed, expressed as Relative Quantity (RQ). The analysis was repeated three times in triplicate and the mean ± S.D. is reported (* p

    Journal: International Journal of Molecular Sciences

    Article Title: Oncogenic H-Ras Expression Induces Fatty Acid Profile Changes in Human Fibroblasts and Extracellular Vesicles

    doi: 10.3390/ijms19113515

    Figure Lengend Snippet: Gene expression analysis of fatty acid-metabolizing enzymes in control and H-RasV12 expressing fibroblasts by qRT-PCR. ( A ) Gene expression analysis of desaturases ( SCD and FADS ), elongases ( ELOVL ) and acyl-coenzyme A synthetases ( ACSL ). ( B ) Gene expression analysis of phospholipases A2 ( PLA2 ). Ten ng of each cDNA were used as template. Reactions were performed in triplicate, using SYBR green technology StepOne RT-PCR machine to detect amplification. The GAPDH gene was used as endogenous control. The fold expression in H-RasV12 fibroblasts with respect to control is displayed, expressed as Relative Quantity (RQ). The analysis was repeated three times in triplicate and the mean ± S.D. is reported (* p

    Article Snippet: RNA was extracted and retro-transcribed as previously described [ ]. cDNA was used to determine the expression of genes listed in . cDNA was used to determine transcript levels by qRT-PCR in a StepOne RT-PCR machine (Applied Biosystems, Foster City, CA, USA) using SYBR® Select Master Mix (Life Technologies, Carlsbad, CA, USA).

    Techniques: Expressing, Quantitative RT-PCR, SYBR Green Assay, Step One RT-PCR, Amplification

    Real-time PCR quantification of mRNA expression levels of candidate genes for plasma HDL concentrations in B6 and NZB mice. Total RNA was extracted from the livers of female mice-fed chow and from mice fed a high-fat diet for 4 weeks. It was then transcribed into cDNA. mRNA expression levels for each candidate gene was quantified with real time PCR by using fluorescent SYBR. Results were normalized to Gapd and expressed as mRNA copies of candidates per 1000 copies of Gapd . ( * P

    Journal: Genome Research

    Article Title: Using Advanced Intercross Lines for High-Resolution Mapping of HDL Cholesterol Quantitative Trait Loci

    doi: 10.1101/gr.1185803

    Figure Lengend Snippet: Real-time PCR quantification of mRNA expression levels of candidate genes for plasma HDL concentrations in B6 and NZB mice. Total RNA was extracted from the livers of female mice-fed chow and from mice fed a high-fat diet for 4 weeks. It was then transcribed into cDNA. mRNA expression levels for each candidate gene was quantified with real time PCR by using fluorescent SYBR. Results were normalized to Gapd and expressed as mRNA copies of candidates per 1000 copies of Gapd . ( * P

    Article Snippet: The primers used are shown in Supplemental . cDNA samples were mixed with primers and SYBR Master Mix (PE Applied Biosystems) in a total volume of 15 μL.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Mouse Assay

    Inhibition assays. Inhibition assays were set up as qPCR reactions using murine genomic DNA; a primer set specific to the mouse- HSD11β1 gene ( S1 Table ); and the PowerUp SYBR Green Master Mix. The reaction mixture was spiked with water (as a control) or extracted RNA or DNA from various kits. Shown are the Cq values for the control vs. reactions spiked with RNA (A) and DNA (B), in duplicate, with error bars representing standard deviations. (C) Fragment distribution of amplified RNA and DNA samples from select kits with corresponding positive controls (fresh PC-3 cells).

    Journal: PLoS ONE

    Article Title: Reliability and performance of commercial RNA and DNA extraction kits for FFPE tissue cores

    doi: 10.1371/journal.pone.0179732

    Figure Lengend Snippet: Inhibition assays. Inhibition assays were set up as qPCR reactions using murine genomic DNA; a primer set specific to the mouse- HSD11β1 gene ( S1 Table ); and the PowerUp SYBR Green Master Mix. The reaction mixture was spiked with water (as a control) or extracted RNA or DNA from various kits. Shown are the Cq values for the control vs. reactions spiked with RNA (A) and DNA (B), in duplicate, with error bars representing standard deviations. (C) Fragment distribution of amplified RNA and DNA samples from select kits with corresponding positive controls (fresh PC-3 cells).

    Article Snippet: Briefly, standard real-time PCR reactions were set up using murine genomic DNA derived from the Ep4 cell-line as the template; a primer set specific to the HSD11β1 gene ( ); and the PowerUp SYBR Green Master Mix (ThermoFisher Scientific, USA).

    Techniques: Inhibition, Real-time Polymerase Chain Reaction, SYBR Green Assay, Amplification

    Promoter methylation is involved in β4 repression during TGFβ-induced EMT. (A,B) Methylation status of the entire CpG island in the β4 promoter in the absence (A) or presence (B) of TGFβ for 11 days. Each circle represents a CpG dinucleotide from the first (1) to the last (66) CpG of randomly selected clones that were sequenced. White circles, unmethylated CpG; black circles, methylated CpG. (C) Immunoblot analysis of β4 expression in the presence of TGFβ alone or TGFβ plus 5-aza for the times indicated. (D) Analysis of β4 mRNA expression by SYBR Green qRT-PCR in the absence of TGFβ (UNT), with TGFβ alone (TGFβ) or with TGFβ and 5-aza (5aza) for 48 hours. Results are reported as the relative expression normalized to that of GAPDH. (E) Bisulfite conversion and methylation analysis of integrin α6 promoter after TGFβ treatment for 11 days as described in A.

    Journal: Journal of Cell Science

    Article Title: Regulation of ?4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition

    doi: 10.1242/jcs.049148

    Figure Lengend Snippet: Promoter methylation is involved in β4 repression during TGFβ-induced EMT. (A,B) Methylation status of the entire CpG island in the β4 promoter in the absence (A) or presence (B) of TGFβ for 11 days. Each circle represents a CpG dinucleotide from the first (1) to the last (66) CpG of randomly selected clones that were sequenced. White circles, unmethylated CpG; black circles, methylated CpG. (C) Immunoblot analysis of β4 expression in the presence of TGFβ alone or TGFβ plus 5-aza for the times indicated. (D) Analysis of β4 mRNA expression by SYBR Green qRT-PCR in the absence of TGFβ (UNT), with TGFβ alone (TGFβ) or with TGFβ and 5-aza (5aza) for 48 hours. Results are reported as the relative expression normalized to that of GAPDH. (E) Bisulfite conversion and methylation analysis of integrin α6 promoter after TGFβ treatment for 11 days as described in A.

    Article Snippet: To assess the expression of specific integrin subunits, E-cadherin, N-cadherin and Snai1 during the EMT by RT-PCR, total RNA (2 μg) was extracted by RNeasy (Qiagen) and reverse transcribed with SuperScriptIII Reverse Transcription system (Invitrogen), and then PCR amplified using either the HotStar Taq Master Mix system (Qiagen) or Power SYBR Green Master Mix (Applied Biosystems), as indicated in the figure legends.

    Techniques: Methylation, Clone Assay, Expressing, SYBR Green Assay, Quantitative RT-PCR

    TGFβ withdrawal reverses EMT and restores both E-cadherin and β4 integrin expression. (A) Phase-contrast photomicrographs of NMuMG cells either untreated (UNT), treated with TGFβ for 11 days (EMT) or treated with TGFβ for 11 days and subsequently in the absence of TGFβ for 13 days (MET). (B) Immunoblot analysis of E-cadherin (E-cad) and β4 integrin (β4) expression under conditions described in A. Tubulin or actin served as a loading control. (C) Analysis of β4 mRNA expression normalized to that of GAPDH in the cells described in A by SYBR Green qRT-PCR. (D) Extracts from the cells described in A were immunoprecipitated with either a nonspecific IgG or a monoclonal antibody against α6 (GOH3), and the immunoprecipitates were blotted with a polyclonal antibody against β4.

    Journal: Journal of Cell Science

    Article Title: Regulation of ?4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition

    doi: 10.1242/jcs.049148

    Figure Lengend Snippet: TGFβ withdrawal reverses EMT and restores both E-cadherin and β4 integrin expression. (A) Phase-contrast photomicrographs of NMuMG cells either untreated (UNT), treated with TGFβ for 11 days (EMT) or treated with TGFβ for 11 days and subsequently in the absence of TGFβ for 13 days (MET). (B) Immunoblot analysis of E-cadherin (E-cad) and β4 integrin (β4) expression under conditions described in A. Tubulin or actin served as a loading control. (C) Analysis of β4 mRNA expression normalized to that of GAPDH in the cells described in A by SYBR Green qRT-PCR. (D) Extracts from the cells described in A were immunoprecipitated with either a nonspecific IgG or a monoclonal antibody against α6 (GOH3), and the immunoprecipitates were blotted with a polyclonal antibody against β4.

    Article Snippet: To assess the expression of specific integrin subunits, E-cadherin, N-cadherin and Snai1 during the EMT by RT-PCR, total RNA (2 μg) was extracted by RNeasy (Qiagen) and reverse transcribed with SuperScriptIII Reverse Transcription system (Invitrogen), and then PCR amplified using either the HotStar Taq Master Mix system (Qiagen) or Power SYBR Green Master Mix (Applied Biosystems), as indicated in the figure legends.

    Techniques: Expressing, SYBR Green Assay, Quantitative RT-PCR, Immunoprecipitation

    PEMF stimulated expression of miR21-5p in differentiated human osteoblasts. Total RNAs from control or PEMF-treated hBMSCs of females (24 × 2, 27, 29, and 30 years old, n = 5) at day 23 of differentiation or (31, 36, 58, and 68 years old) at day 23 or 33 of differentiation were isolated and subjected to RT-qPCR using the miScript II kit with miScript HiSpec Buffer and miScript SYBR Green PCR Kit. snoR10-1 was used to normalize miR21-5p expression and the expression is shown as a percentage of the relevant control samples. ∗ indicates significant increase compared to control using one-way ANOVA.

    Journal: Stem Cells International

    Article Title: Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-β Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation

    doi: 10.1155/2017/2450327

    Figure Lengend Snippet: PEMF stimulated expression of miR21-5p in differentiated human osteoblasts. Total RNAs from control or PEMF-treated hBMSCs of females (24 × 2, 27, 29, and 30 years old, n = 5) at day 23 of differentiation or (31, 36, 58, and 68 years old) at day 23 or 33 of differentiation were isolated and subjected to RT-qPCR using the miScript II kit with miScript HiSpec Buffer and miScript SYBR Green PCR Kit. snoR10-1 was used to normalize miR21-5p expression and the expression is shown as a percentage of the relevant control samples. ∗ indicates significant increase compared to control using one-way ANOVA.

    Article Snippet: The Power SYBR green master mix kit for PCR reactions was purchased from Invitrogen.

    Techniques: Expressing, Isolation, Quantitative RT-PCR, SYBR Green Assay, Polymerase Chain Reaction