Journal: BMC gastroenterology

Article Title: The impact of probiotic supplementation on gastric motility and nutrient absorption in elderly patients with Gastrointestinal disorders.

doi: 10.1186/s12876-025-03740-2

Figure Lengend Snippet: Fig. 3 Comparison of intestinal permeability markers levels between two groups (after treatment 7d). (A) Occludin; (B) Zonulin; (C) LBP. LBP: Lipopolysac charide Binding Protein. *: P < 0.05; **: P < 0.01.Paired t-tests were used to compare pre- vs. post-treatment values within each group; independent t-tests were used for between-group comparisons at day 7

Article Snippet: The specific ELISA kits employed included: the human zonulin kit (EKC 36091, Biomatik USA, LLC; Wilmington, DE, USA), the human occludin kit (NBP2-80305, Novus Biologicals, LLC; Centennial, CO, USA), and the human LBP kit (DY870-05, R&D Systems, Inc.; Minneapolis, MN, USA).

Techniques: Comparison, Permeability, Binding Assay

Journal: Turkish journal of medical sciences

Article Title: Leaky gut and inflammatory biomarkers in a medication overuse headache model in male rats.

doi: 10.55730/1300-0144.5763

Figure Lengend Snippet: Figure 5. a) Brain IL-17 and b) HMGB1 levels were significantly higher in the NSAID group compared to its vehicle (p = 0.016 and p = 0.016, respectively). c) Brain IL-6 levels were similar between the two groups (p = 0.67). d) Significantly greater serum LBP and e) occludin levels were found in the NSAID group compared to the vehicle (p = 0.006 and p = 0.016, respectively). *p < 0.05 vs. controls.

Article Snippet: Serum LBP (E-EL-R0589) and occludin levels (E-EL-R2503) and brain tissue HMGB1 (E-EL-R0505) were measured with sandwich-type rat ELISA kits from Elabscience Biotechnology Inc., Houston, TX, USA.

Techniques:

Journal: Cell genomics

Article Title: Genetic adaptation to pathogens and increased risk of inflammatory disorders in post-Neolithic Europe.

doi: 10.1016/j.xgen.2022.100248

Figure Lengend Snippet: Figure 5. Functional impact of negatively selected variants (A–D) Functional study of the LBP D283G variant. (A) qRT-PCR for LBP on cDNA from HEK293T cells non-transfected (NT) or transfected with an empty plasmid (EV), or plasmids encoding ancestral or derived LBP. Dots indicate three independent experiments, and the height of each bar their mean values. (B) Western blot of whole-cell lysates or cell culture supernatants from HEK293T cells either left NT, transfected with an EV, or C-terminally tagged plasmids expressing ancestral or derived LBP forms. LBP was detected with a polyclonal anti-LBP antibody and an antibody against the C-terminal DDK tag. An antibody against GADPH was used as loading control. The results shown are representative of three independent experiments. (C) LBP concentration in cell culture supernatants from transfected HEK293T cells as measured by ELISA. Dots indicate three independent experiments, and the height of each bar their mean values. (D) Binding of ancestral or derived LBP collected from cell culture supernatant at increasing concentrations to LPS and Pam2CSK4 assessed by a binding assay. Each point represents the mean of two biological replicates ± SD. (E and F) Functional study of the IL23R R381Q variant. (E) T cell blasts from seven healthy controls homozygous for the ancestral variant, seven patients ho- mozygous for the derived IL23R R381Q variant, and an IL-12Rb1-deficient patient were left unstimulated or were stimulated with IL-23 (ranging from 1 to 100 ng/ mL) or IFN-a2b as a positive control. STAT3 phosphorylation was evaluated by flow cytometry. A Wilcoxon test was used to assess whether observed control and IL23R R381Q-derived values belong to the same population. *p < 0.05, **p < 0.01. (F) T cell blasts from nine healthy controls homozygous for the ancestral IL23R variant, two individuals homozygous for IL23R R381Q, two homozygous for TYK2 P1104A, one TYK2/ patient, and one IL-12Rb1/ patient were stimulated with IL-1b for 24 h and with IL-12 or IL-23 for 6 h. Then, RNA sequencing was performed. The impact of the IL23R R381Q on the IFN-g network was dissected by performing gene set enrichment analysis (GSEA: fgsea) using hallmark gene sets (http://www.gsea-msigdb.org/) and a mean log2 fold-change (log2FC) in each condition. Dot heatmaps are shown for the 10 most strongly affected gene sets in healthy controls on IL-23 plus IL-1b stimulation when compared with IL-1b stimulation. (G–I) Functional study of the TLR3 L412F variant. (G) Principal-component analysis (PCA) of RNA sequencing-quantified gene expression for two controls ho- mozygous for the ancestral TLR3 variant, four TLR3 L412F homozygotes, and one complete TLR3-deficient primary fibroblast cell left non-stimulated (NS) or

Article Snippet: Membranes were probed by incubation for 1 h at room temperature with antibodies against LBP (#AF870-SP, R&D Systems, 1:2,000), DDK (#A8592, Merck, 1:10,000) and GAPDH (#sc-47724, Santa Cruz Biotechnology, 1:5,000).

Techniques: Functional Assay, Variant Assay, Quantitative RT-PCR, Transfection, Plasmid Preparation, Derivative Assay, Western Blot, Cell Culture, Expressing, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, Binding Assay, Positive Control, Phospho-proteomics, Cytometry, RNA Sequencing, Gene Expression

Journal: Shock

Article Title: Lipopolysaccharide-Induced Cytokine Secretion from In Vitro Mouse Slow and Fast Limb Muscle

doi: 10.1097/shk.0000000000001891

Figure Lengend Snippet: FIG. 1. Representative cytokines secreted from isolated SOL in response to 1 mg/mL LPS dissolved in Krebs buffer (n ¼ 8 per group), Stats are Wilcoxon Signed ranks for matched pairs *P < 0.05, ** P < 0.01. T0 ¼ the beginning of LPS exposure, T1 ¼ 1 h after LPS exposure, T2 ¼ 2 h after LPS.

Article Snippet: Tests of SOL muscles were repeated in response to 1 mg/mL LPS in the presence of 1% C57BL/6 cytokine-free plasma (IGMS-C57-N, Innovative Research, Novi, Mich), 1 mg/mL LPS þ 1 mg/mL LPS-binding protein (LBP; 6635-LP/CF, R&D Systems, Minneapolis, Minn) or Krebs buffer alone.

Techniques: Isolation

Journal: Shock

Article Title: Lipopolysaccharide-Induced Cytokine Secretion from In Vitro Mouse Slow and Fast Limb Muscle

doi: 10.1097/shk.0000000000001891

Figure Lengend Snippet: FIG. 2. Representative cytokine secretory responses in isolated mouse SOL and mouse extensor digitorum longus (EDL) in response to 1 mg/mL LPS in Krebs buffer þ 1% serum. Stats used Wilcoxon- Signed ranks for matched pairs from time 0 to T1, and T1 to T2. ** ¼ P < 0.01, *** P < 0.001, n ¼ 10 for SOL, n ¼ 17 for EDL. T0 ¼ the beginning of LPS exposure, T1 ¼ 1 h after LPS exposure, T2 ¼ 2 h after LPS.

Article Snippet: Tests of SOL muscles were repeated in response to 1 mg/mL LPS in the presence of 1% C57BL/6 cytokine-free plasma (IGMS-C57-N, Innovative Research, Novi, Mich), 1 mg/mL LPS þ 1 mg/mL LPS-binding protein (LBP; 6635-LP/CF, R&D Systems, Minneapolis, Minn) or Krebs buffer alone.

Techniques: Isolation

Journal: Shock

Article Title: Lipopolysaccharide-Induced Cytokine Secretion from In Vitro Mouse Slow and Fast Limb Muscle

doi: 10.1097/shk.0000000000001891

Figure Lengend Snippet: FIG. 4. Comparisons of total cytokine secretion (log scale) of SOL vs. EDL at T2. Muscles were incubated in Krebs þ 1% plasma. T2 ¼ accumulated cytokines at the end of the 2 h exposure to LPS. Means SD, n ¼ 11 for SOL, n ¼ 16 for EDL. Comparisons are two sample t for parametric and Mann–Whitney for nonparametric samples. * ¼ P < 0.05, ** P < 0.01

Article Snippet: Tests of SOL muscles were repeated in response to 1 mg/mL LPS in the presence of 1% C57BL/6 cytokine-free plasma (IGMS-C57-N, Innovative Research, Novi, Mich), 1 mg/mL LPS þ 1 mg/mL LPS-binding protein (LBP; 6635-LP/CF, R&D Systems, Minneapolis, Minn) or Krebs buffer alone.

Techniques: Muscles, Incubation, Clinical Proteomics, MANN-WHITNEY

Journal: Innate immunity

Article Title: Monitoring of endothelial cell activation in experimental sepsis with a two-step cell culture model.

doi: 10.1177/1753425909341885

Figure Lengend Snippet: Fig. 1. Secretion of TNF-a from THP-1 after stimulation with 10 ng/ml LPS in different media. Cultures of THP-1 were stimulated in medium M199 containing 10% FBS or 10% human plasma with or without addition of sCD14 (1 mg/ml) and LBP (0.5 mg/ml). The concentrations of TNF-a are expressed as mean SD (n ¼ 3).

Article Snippet: Recombinant human LPS-binding protein (LBP) and recombinant human sCD14 were from R&D Systems (Minneapolis, MN, USA).

Techniques: Clinical Proteomics