large klenow fragment New England Biolabs Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    New England Biolabs large klenow fragment
    A flowchart showing the manipulation steps in the preparation of genomic <t>DNA</t> for IPCR. The genomic DNA was subjected to RE digestions, <t>Klenow</t> fill-in and ligation prior to IPCR as reported before [ 80 ]
    Large Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/large klenow fragment/product/New England Biolabs
    Average 90 stars, based on 1280 article reviews
    Price from $9.99 to $1999.99
    large klenow fragment - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    99
    Thermo Fisher klenow dna polymerase
    A flowchart showing the manipulation steps in the preparation of genomic <t>DNA</t> for IPCR. The genomic DNA was subjected to RE digestions, <t>Klenow</t> fill-in and ligation prior to IPCR as reported before [ 80 ]
    Klenow Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 413 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/klenow dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 413 article reviews
    Price from $9.99 to $1999.99
    klenow dna polymerase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs dna polymerase i klenow fragment
    The VEX complex associates with both the active-VSG and the Spliced Leader ( SL )-locus in a cell cycle and developmental stage-dependent manner. a-b, Immunofluorescence-based colocalisation studies of VEX1 myc / Pol I and GFP VEX2 / tSNAP myc in bloodstream form cells. tSNAP and Pol I were used as markers for the SL-RNA and VSG transcription compartments, respectively. The stacked bar graphs depict proportions of nuclei with overlapping, adjacent or separate signals and values are averages of two independent experiments (≥100 nuclei for G1 and S phase cells); detailed n and p values are provided in Data S1 sheet 3. c, VEX1 myc chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq) analysis. The circle plot represents log2 fold change of ChIP versus Input of non-overlapping 1 kbp bins of the 11 megabase chromosomes; outside track shows tandem arrays (red) and the SL-RNA locus (black). An inset zooming on the SL-RNA locus is depicted: metagene plot (left-hand side) and heat-map (right-hand side) of SL-gene loci. Bin size 300 bp. d, Immunofluorescence-based colocalisation studies of tSNAP myc and a nucleolar marker (NOG1) in bloodstream forms. e, Localisation of tSNAP GFP and colocalisation studies of VEX1 myc or myc VEX2 and Pol I in procyclic forms (insect-stage), using immunofluorescence. Procyclic forms do not express VSGs whereas procyclins are the major surface glycoprotein. Images in a-b / d-e were obtained with super resolution microscopy and correspond to maximal 3D projections of stacks of 0.1 μm slices; <t>DNA</t> was counter-stained with DAPI; scale bars 2 μm. f, Western-blot analysis of VEX1 myc , myc VEX2 and tSNAP myc before and after sinefungin treatment (5 μg ml -1 for 30 min at 37°C), which blocks trans -splicing in trypanosomes. Data in a-b and d-f are representative of at least two independent biological replicates and two independent experiments.
    Dna Polymerase I Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1004 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase i klenow fragment/product/New England Biolabs
    Average 99 stars, based on 1004 article reviews
    Price from $9.99 to $1999.99
    dna polymerase i klenow fragment - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs dna polymerase
    The VEX complex associates with both the active-VSG and the Spliced Leader ( SL )-locus in a cell cycle and developmental stage-dependent manner. a-b, Immunofluorescence-based colocalisation studies of VEX1 myc / Pol I and GFP VEX2 / tSNAP myc in bloodstream form cells. tSNAP and Pol I were used as markers for the SL-RNA and VSG transcription compartments, respectively. The stacked bar graphs depict proportions of nuclei with overlapping, adjacent or separate signals and values are averages of two independent experiments (≥100 nuclei for G1 and S phase cells); detailed n and p values are provided in Data S1 sheet 3. c, VEX1 myc chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq) analysis. The circle plot represents log2 fold change of ChIP versus Input of non-overlapping 1 kbp bins of the 11 megabase chromosomes; outside track shows tandem arrays (red) and the SL-RNA locus (black). An inset zooming on the SL-RNA locus is depicted: metagene plot (left-hand side) and heat-map (right-hand side) of SL-gene loci. Bin size 300 bp. d, Immunofluorescence-based colocalisation studies of tSNAP myc and a nucleolar marker (NOG1) in bloodstream forms. e, Localisation of tSNAP GFP and colocalisation studies of VEX1 myc or myc VEX2 and Pol I in procyclic forms (insect-stage), using immunofluorescence. Procyclic forms do not express VSGs whereas procyclins are the major surface glycoprotein. Images in a-b / d-e were obtained with super resolution microscopy and correspond to maximal 3D projections of stacks of 0.1 μm slices; <t>DNA</t> was counter-stained with DAPI; scale bars 2 μm. f, Western-blot analysis of VEX1 myc , myc VEX2 and tSNAP myc before and after sinefungin treatment (5 μg ml -1 for 30 min at 37°C), which blocks trans -splicing in trypanosomes. Data in a-b and d-f are representative of at least two independent biological replicates and two independent experiments.
    Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8088 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase/product/New England Biolabs
    Average 99 stars, based on 8088 article reviews
    Price from $9.99 to $1999.99
    dna polymerase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs end repair reaction
    The VEX complex associates with both the active-VSG and the Spliced Leader ( SL )-locus in a cell cycle and developmental stage-dependent manner. a-b, Immunofluorescence-based colocalisation studies of VEX1 myc / Pol I and GFP VEX2 / tSNAP myc in bloodstream form cells. tSNAP and Pol I were used as markers for the SL-RNA and VSG transcription compartments, respectively. The stacked bar graphs depict proportions of nuclei with overlapping, adjacent or separate signals and values are averages of two independent experiments (≥100 nuclei for G1 and S phase cells); detailed n and p values are provided in Data S1 sheet 3. c, VEX1 myc chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq) analysis. The circle plot represents log2 fold change of ChIP versus Input of non-overlapping 1 kbp bins of the 11 megabase chromosomes; outside track shows tandem arrays (red) and the SL-RNA locus (black). An inset zooming on the SL-RNA locus is depicted: metagene plot (left-hand side) and heat-map (right-hand side) of SL-gene loci. Bin size 300 bp. d, Immunofluorescence-based colocalisation studies of tSNAP myc and a nucleolar marker (NOG1) in bloodstream forms. e, Localisation of tSNAP GFP and colocalisation studies of VEX1 myc or myc VEX2 and Pol I in procyclic forms (insect-stage), using immunofluorescence. Procyclic forms do not express VSGs whereas procyclins are the major surface glycoprotein. Images in a-b / d-e were obtained with super resolution microscopy and correspond to maximal 3D projections of stacks of 0.1 μm slices; <t>DNA</t> was counter-stained with DAPI; scale bars 2 μm. f, Western-blot analysis of VEX1 myc , myc VEX2 and tSNAP myc before and after sinefungin treatment (5 μg ml -1 for 30 min at 37°C), which blocks trans -splicing in trypanosomes. Data in a-b and d-f are representative of at least two independent biological replicates and two independent experiments.
    End Repair Reaction, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/end repair reaction/product/New England Biolabs
    Average 99 stars, based on 97 article reviews
    Price from $9.99 to $1999.99
    end repair reaction - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    A flowchart showing the manipulation steps in the preparation of genomic DNA for IPCR. The genomic DNA was subjected to RE digestions, Klenow fill-in and ligation prior to IPCR as reported before [ 80 ]

    Journal: Human Genomics

    Article Title: Oxidative stress-induced chromosome breaks within the ABL gene: a model for chromosome rearrangement in nasopharyngeal carcinoma

    doi: 10.1186/s40246-018-0160-8

    Figure Lengend Snippet: A flowchart showing the manipulation steps in the preparation of genomic DNA for IPCR. The genomic DNA was subjected to RE digestions, Klenow fill-in and ligation prior to IPCR as reported before [ 80 ]

    Article Snippet: DNA Polymerase I Large (Klenow) Fragment, restriction enzymes and T4 DNA Ligase were obtained from New England Biolabs (NEB), USA. dNTP mix was purchased from Promega, USA.

    Techniques: Ligation

    The VEX complex associates with both the active-VSG and the Spliced Leader ( SL )-locus in a cell cycle and developmental stage-dependent manner. a-b, Immunofluorescence-based colocalisation studies of VEX1 myc / Pol I and GFP VEX2 / tSNAP myc in bloodstream form cells. tSNAP and Pol I were used as markers for the SL-RNA and VSG transcription compartments, respectively. The stacked bar graphs depict proportions of nuclei with overlapping, adjacent or separate signals and values are averages of two independent experiments (≥100 nuclei for G1 and S phase cells); detailed n and p values are provided in Data S1 sheet 3. c, VEX1 myc chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq) analysis. The circle plot represents log2 fold change of ChIP versus Input of non-overlapping 1 kbp bins of the 11 megabase chromosomes; outside track shows tandem arrays (red) and the SL-RNA locus (black). An inset zooming on the SL-RNA locus is depicted: metagene plot (left-hand side) and heat-map (right-hand side) of SL-gene loci. Bin size 300 bp. d, Immunofluorescence-based colocalisation studies of tSNAP myc and a nucleolar marker (NOG1) in bloodstream forms. e, Localisation of tSNAP GFP and colocalisation studies of VEX1 myc or myc VEX2 and Pol I in procyclic forms (insect-stage), using immunofluorescence. Procyclic forms do not express VSGs whereas procyclins are the major surface glycoprotein. Images in a-b / d-e were obtained with super resolution microscopy and correspond to maximal 3D projections of stacks of 0.1 μm slices; DNA was counter-stained with DAPI; scale bars 2 μm. f, Western-blot analysis of VEX1 myc , myc VEX2 and tSNAP myc before and after sinefungin treatment (5 μg ml -1 for 30 min at 37°C), which blocks trans -splicing in trypanosomes. Data in a-b and d-f are representative of at least two independent biological replicates and two independent experiments.

    Journal: bioRxiv

    Article Title: Antigenic variation by switching inter-chromosomal interactions with an RNA splicing locus in trypanosomes

    doi: 10.1101/2020.01.27.921452

    Figure Lengend Snippet: The VEX complex associates with both the active-VSG and the Spliced Leader ( SL )-locus in a cell cycle and developmental stage-dependent manner. a-b, Immunofluorescence-based colocalisation studies of VEX1 myc / Pol I and GFP VEX2 / tSNAP myc in bloodstream form cells. tSNAP and Pol I were used as markers for the SL-RNA and VSG transcription compartments, respectively. The stacked bar graphs depict proportions of nuclei with overlapping, adjacent or separate signals and values are averages of two independent experiments (≥100 nuclei for G1 and S phase cells); detailed n and p values are provided in Data S1 sheet 3. c, VEX1 myc chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq) analysis. The circle plot represents log2 fold change of ChIP versus Input of non-overlapping 1 kbp bins of the 11 megabase chromosomes; outside track shows tandem arrays (red) and the SL-RNA locus (black). An inset zooming on the SL-RNA locus is depicted: metagene plot (left-hand side) and heat-map (right-hand side) of SL-gene loci. Bin size 300 bp. d, Immunofluorescence-based colocalisation studies of tSNAP myc and a nucleolar marker (NOG1) in bloodstream forms. e, Localisation of tSNAP GFP and colocalisation studies of VEX1 myc or myc VEX2 and Pol I in procyclic forms (insect-stage), using immunofluorescence. Procyclic forms do not express VSGs whereas procyclins are the major surface glycoprotein. Images in a-b / d-e were obtained with super resolution microscopy and correspond to maximal 3D projections of stacks of 0.1 μm slices; DNA was counter-stained with DAPI; scale bars 2 μm. f, Western-blot analysis of VEX1 myc , myc VEX2 and tSNAP myc before and after sinefungin treatment (5 μg ml -1 for 30 min at 37°C), which blocks trans -splicing in trypanosomes. Data in a-b and d-f are representative of at least two independent biological replicates and two independent experiments.

    Article Snippet: For end-repair and biotin removal from un-ligated ends, 70 μl of end-repair mix was added (1× Ligation buffer (NEB), 357 μM dNTPs, 25U T4 PNK (NEB, M0201), 7.5U T4 DNA polymerase I (NEB, M0203), 2.5U DNA polymerase I, large (Klenow) fragment (NEB, M0210)) and incubated for 30 min at 20 °C and 20 min at 75 °C.

    Techniques: Immunofluorescence, Chromatin Immunoprecipitation, Next-Generation Sequencing, Marker, Microscopy, Staining, Western Blot

    The active VSG expression site (ES) stably interacts with the spliced leader RNA (SL) array. a, Hi-C (virtual 4C) analysis, viewpoints: active VSG gene in ES1 ( VSG-2 , top panel) and silent VSG gene in ES 3 ( VSG-6 , bottom panel). Relative interaction frequencies between the viewpoint and the 11 megabase chromosomes are shown. Chromosome cores, dark grey; sub-telomeric regions, light grey. The hemizygous sub-telomeric regions are displayed in the following order: 5’(haplotype A)–5’(haplotype B)–diploid chromosome core–3’(haplotype A)– 3’(haplotype B). Bin size 50 kb. b, Virtual 4C analysis, viewpoints: active VSG gene in ES 1 and inactive VSG genes in ES 3, 4, 5, 7, 11, 13 and 15. Relative interaction frequencies between the viewpoint and the SL-RNA locus on the right arm of chr. 9 is plotted. Bin size 20 kb. The analyses in a - b are based on Hi-C experiments with VSG-2 expressing cells (n=2, average interaction frequencies are shown). c, Immunofluorescence-based colocalisation studies of tSNAP myc (SL-RNA locus marker – SL-RNA transcription compartment) and a nucleolar and active- VSG transcription compartment marker (Pol I, largest subunit) using super resolution microscopy. The stacked bar graph depicts proportions of G1 or S phase nuclei with overlapping, adjacent or separate signals for the SL-RNA and VSG transcription compartments. Values are averages of three independent experiments and representative of two independent biological replicates (≥100 G1 or S phase nuclei); error bars, SD. Detailed n and p values are provided in Data S1 sheet 3. DNA was counter-stained with DAPI; the images correspond to maximal 3D projections of stacks of 0.1 μm slices; scale bars 2 μm. N, nucleus; K, kinetoplast (mitochondrial genome).

    Journal: bioRxiv

    Article Title: Antigenic variation by switching inter-chromosomal interactions with an RNA splicing locus in trypanosomes

    doi: 10.1101/2020.01.27.921452

    Figure Lengend Snippet: The active VSG expression site (ES) stably interacts with the spliced leader RNA (SL) array. a, Hi-C (virtual 4C) analysis, viewpoints: active VSG gene in ES1 ( VSG-2 , top panel) and silent VSG gene in ES 3 ( VSG-6 , bottom panel). Relative interaction frequencies between the viewpoint and the 11 megabase chromosomes are shown. Chromosome cores, dark grey; sub-telomeric regions, light grey. The hemizygous sub-telomeric regions are displayed in the following order: 5’(haplotype A)–5’(haplotype B)–diploid chromosome core–3’(haplotype A)– 3’(haplotype B). Bin size 50 kb. b, Virtual 4C analysis, viewpoints: active VSG gene in ES 1 and inactive VSG genes in ES 3, 4, 5, 7, 11, 13 and 15. Relative interaction frequencies between the viewpoint and the SL-RNA locus on the right arm of chr. 9 is plotted. Bin size 20 kb. The analyses in a - b are based on Hi-C experiments with VSG-2 expressing cells (n=2, average interaction frequencies are shown). c, Immunofluorescence-based colocalisation studies of tSNAP myc (SL-RNA locus marker – SL-RNA transcription compartment) and a nucleolar and active- VSG transcription compartment marker (Pol I, largest subunit) using super resolution microscopy. The stacked bar graph depicts proportions of G1 or S phase nuclei with overlapping, adjacent or separate signals for the SL-RNA and VSG transcription compartments. Values are averages of three independent experiments and representative of two independent biological replicates (≥100 G1 or S phase nuclei); error bars, SD. Detailed n and p values are provided in Data S1 sheet 3. DNA was counter-stained with DAPI; the images correspond to maximal 3D projections of stacks of 0.1 μm slices; scale bars 2 μm. N, nucleus; K, kinetoplast (mitochondrial genome).

    Article Snippet: For end-repair and biotin removal from un-ligated ends, 70 μl of end-repair mix was added (1× Ligation buffer (NEB), 357 μM dNTPs, 25U T4 PNK (NEB, M0201), 7.5U T4 DNA polymerase I (NEB, M0203), 2.5U DNA polymerase I, large (Klenow) fragment (NEB, M0210)) and incubated for 30 min at 20 °C and 20 min at 75 °C.

    Techniques: Expressing, Stable Transfection, Hi-C, Immunofluorescence, Marker, Microscopy, Staining

    Pol I and tSNAP expression and localisation following knockdown of the VEX complex. a, Immunofluorescence-based analysis of VSG expression following tetracycline (Tet) inducible VEX1 knockdown, VEX2 knockdown or VEX1/VEX2 knockdown. VSG-2 (magenta) is the active- VSG and VSG-6 (green) is a silent- VSG in this strain. The stacked bar graph depicts percentages of VSG-2 single positive cells and VSG-2/VSG-6 double positive cells; values are averages of two independent experiments and two biological replicates. DNA was counter-stained with DAPI; scale bar 2 μm. b, Western-blot analysis of VEX2, Pol I, tSNAP myc , VSG-6 and VSG-2 expression following VEX1, VEX2 or VEX1/VEX2 knockdown. EF1α was used as a loading control. The data is representative of two independent experiments and two biological replicates. c-d , Immunofluorescence-based colocalisation studies of tSNAP myc and a nucleolar and active- VSG marker (Pol I, large subunit). The stacked bar graph in c depicts proportions of G1 nuclei with tSNAP myc / Pol I overlapping, adjacent or separate signals following tetracycline (Tet) inducible VEX1 (48 h), VEX2 (12 h) or VEX1/VEX2 knockdown (12 h). tSNAP myc / active- VSG localisation were not monitored beyond 12 h following VEX2 and VEX1/2 knockdown as Pol I signal drops below detection at later time-points. The values are averages of two independent experiments and two biological replicates (≥100 G1 nuclei). In the box plot in d , the distance between the edges of the ESB and tSNAP foci was measured in > 81 G1 nuclei. The centre lines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend from maximal to the minimal values; all data points are shown. In a/c , error bars, SD. In c-d , knockdown conditions were compared to parental cells using two-tailed paired ( c ) or unpaired ( d ) Student’s t -tests: *, p

    Journal: bioRxiv

    Article Title: Antigenic variation by switching inter-chromosomal interactions with an RNA splicing locus in trypanosomes

    doi: 10.1101/2020.01.27.921452

    Figure Lengend Snippet: Pol I and tSNAP expression and localisation following knockdown of the VEX complex. a, Immunofluorescence-based analysis of VSG expression following tetracycline (Tet) inducible VEX1 knockdown, VEX2 knockdown or VEX1/VEX2 knockdown. VSG-2 (magenta) is the active- VSG and VSG-6 (green) is a silent- VSG in this strain. The stacked bar graph depicts percentages of VSG-2 single positive cells and VSG-2/VSG-6 double positive cells; values are averages of two independent experiments and two biological replicates. DNA was counter-stained with DAPI; scale bar 2 μm. b, Western-blot analysis of VEX2, Pol I, tSNAP myc , VSG-6 and VSG-2 expression following VEX1, VEX2 or VEX1/VEX2 knockdown. EF1α was used as a loading control. The data is representative of two independent experiments and two biological replicates. c-d , Immunofluorescence-based colocalisation studies of tSNAP myc and a nucleolar and active- VSG marker (Pol I, large subunit). The stacked bar graph in c depicts proportions of G1 nuclei with tSNAP myc / Pol I overlapping, adjacent or separate signals following tetracycline (Tet) inducible VEX1 (48 h), VEX2 (12 h) or VEX1/VEX2 knockdown (12 h). tSNAP myc / active- VSG localisation were not monitored beyond 12 h following VEX2 and VEX1/2 knockdown as Pol I signal drops below detection at later time-points. The values are averages of two independent experiments and two biological replicates (≥100 G1 nuclei). In the box plot in d , the distance between the edges of the ESB and tSNAP foci was measured in > 81 G1 nuclei. The centre lines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend from maximal to the minimal values; all data points are shown. In a/c , error bars, SD. In c-d , knockdown conditions were compared to parental cells using two-tailed paired ( c ) or unpaired ( d ) Student’s t -tests: *, p

    Article Snippet: For end-repair and biotin removal from un-ligated ends, 70 μl of end-repair mix was added (1× Ligation buffer (NEB), 357 μM dNTPs, 25U T4 PNK (NEB, M0201), 7.5U T4 DNA polymerase I (NEB, M0203), 2.5U DNA polymerase I, large (Klenow) fragment (NEB, M0210)) and incubated for 30 min at 20 °C and 20 min at 75 °C.

    Techniques: Expressing, Immunofluorescence, Staining, Western Blot, Marker, Two Tailed Test