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    Phylogeny of 34 most abundant operational taxonomic units (OTUs) of ur e C (corresponding to the species level). ur e C reads obtained by <t>amplicon</t> sequencing on the Illumina MiSeq platform were clustered into species-level OTUs with ≥91% sequence identity, and a phylogenetic tree was constructed by the maximum likelihood method with the Jones-Taylor-Thornton model by means of the ur e C sequence of Canavalia ensiformis (M65260) as an outgroup. Branching points that support probability > 80% in the bootstrap analyses (based on 500 replicates) are shown as filled circles. The heatmap shows the relative abundance of species-level OTUs of ure C in soil samples 1 to 7. The scale bar represents 20% sequence divergence. Nucleotide sequence accession numbers are indicated in parentheses.
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    Phylogeny of 34 most abundant operational taxonomic units (OTUs) of ur e C (corresponding to the species level). ur e C reads obtained by <t>amplicon</t> sequencing on the Illumina MiSeq platform were clustered into species-level OTUs with ≥91% sequence identity, and a phylogenetic tree was constructed by the maximum likelihood method with the Jones-Taylor-Thornton model by means of the ur e C sequence of Canavalia ensiformis (M65260) as an outgroup. Branching points that support probability > 80% in the bootstrap analyses (based on 500 replicates) are shown as filled circles. The heatmap shows the relative abundance of species-level OTUs of ure C in soil samples 1 to 7. The scale bar represents 20% sequence divergence. Nucleotide sequence accession numbers are indicated in parentheses.
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    Phylogeny of 34 most abundant operational taxonomic units (OTUs) of ur e C (corresponding to the species level). ur e C reads obtained by <t>amplicon</t> sequencing on the Illumina MiSeq platform were clustered into species-level OTUs with ≥91% sequence identity, and a phylogenetic tree was constructed by the maximum likelihood method with the Jones-Taylor-Thornton model by means of the ur e C sequence of Canavalia ensiformis (M65260) as an outgroup. Branching points that support probability > 80% in the bootstrap analyses (based on 500 replicates) are shown as filled circles. The heatmap shows the relative abundance of species-level OTUs of ure C in soil samples 1 to 7. The scale bar represents 20% sequence divergence. Nucleotide sequence accession numbers are indicated in parentheses.
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    Phylogeny of 34 most abundant operational taxonomic units (OTUs) of ur e C (corresponding to the species level). ur e C reads obtained by <t>amplicon</t> sequencing on the Illumina MiSeq platform were clustered into species-level OTUs with ≥91% sequence identity, and a phylogenetic tree was constructed by the maximum likelihood method with the Jones-Taylor-Thornton model by means of the ur e C sequence of Canavalia ensiformis (M65260) as an outgroup. Branching points that support probability > 80% in the bootstrap analyses (based on 500 replicates) are shown as filled circles. The heatmap shows the relative abundance of species-level OTUs of ure C in soil samples 1 to 7. The scale bar represents 20% sequence divergence. Nucleotide sequence accession numbers are indicated in parentheses.
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    Phylogeny of 34 most abundant operational taxonomic units (OTUs) of ur e C (corresponding to the species level). ur e C reads obtained by <t>amplicon</t> sequencing on the Illumina MiSeq platform were clustered into species-level OTUs with ≥91% sequence identity, and a phylogenetic tree was constructed by the maximum likelihood method with the Jones-Taylor-Thornton model by means of the ur e C sequence of Canavalia ensiformis (M65260) as an outgroup. Branching points that support probability > 80% in the bootstrap analyses (based on 500 replicates) are shown as filled circles. The heatmap shows the relative abundance of species-level OTUs of ure C in soil samples 1 to 7. The scale bar represents 20% sequence divergence. Nucleotide sequence accession numbers are indicated in parentheses.
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    Phylogeny of 34 most abundant operational taxonomic units (OTUs) of ur e C (corresponding to the species level). ur e C reads obtained by <t>amplicon</t> sequencing on the Illumina MiSeq platform were clustered into species-level OTUs with ≥91% sequence identity, and a phylogenetic tree was constructed by the maximum likelihood method with the Jones-Taylor-Thornton model by means of the ur e C sequence of Canavalia ensiformis (M65260) as an outgroup. Branching points that support probability > 80% in the bootstrap analyses (based on 500 replicates) are shown as filled circles. The heatmap shows the relative abundance of species-level OTUs of ure C in soil samples 1 to 7. The scale bar represents 20% sequence divergence. Nucleotide sequence accession numbers are indicated in parentheses.
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    Image Search Results


    Phylogeny of 34 most abundant operational taxonomic units (OTUs) of ur e C (corresponding to the species level). ur e C reads obtained by amplicon sequencing on the Illumina MiSeq platform were clustered into species-level OTUs with ≥91% sequence identity, and a phylogenetic tree was constructed by the maximum likelihood method with the Jones-Taylor-Thornton model by means of the ur e C sequence of Canavalia ensiformis (M65260) as an outgroup. Branching points that support probability > 80% in the bootstrap analyses (based on 500 replicates) are shown as filled circles. The heatmap shows the relative abundance of species-level OTUs of ure C in soil samples 1 to 7. The scale bar represents 20% sequence divergence. Nucleotide sequence accession numbers are indicated in parentheses.

    Journal: Microbes and Environments

    Article Title: Ureolytic Prokaryotes in Soil: Community Abundance and Diversity

    doi: 10.1264/jsme2.ME17188

    Figure Lengend Snippet: Phylogeny of 34 most abundant operational taxonomic units (OTUs) of ur e C (corresponding to the species level). ur e C reads obtained by amplicon sequencing on the Illumina MiSeq platform were clustered into species-level OTUs with ≥91% sequence identity, and a phylogenetic tree was constructed by the maximum likelihood method with the Jones-Taylor-Thornton model by means of the ur e C sequence of Canavalia ensiformis (M65260) as an outgroup. Branching points that support probability > 80% in the bootstrap analyses (based on 500 replicates) are shown as filled circles. The heatmap shows the relative abundance of species-level OTUs of ure C in soil samples 1 to 7. The scale bar represents 20% sequence divergence. Nucleotide sequence accession numbers are indicated in parentheses.

    Article Snippet: In the present study, we aimed to investigate the abundance and diversity of ur eC sequences in various types of soils using a set of proper ur eC primers and amplicon sequencing on an Illumina MiSeq sequencer.

    Techniques: Amplification, Sequencing, Construct