lambda phage dna Promega Search Results


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  • 90
    New England Biolabs bacteriophage lambda dna
    Recovery of nucleic acids and polyphosphate (polyP) using various purification methods. Known quantities of bacteriophage <t>lambda</t> <t>DNA</t> (DNA, striped bars), polyG (RNA, white bars), or long-chain polyP (black bars) were processed for nucleic acid purification using Qiaquick, DNeasy, or TRIzol kits, following the manufacturer’s instructions, or with phenol/chloroform extraction. Nucleic acid recovery was quantified using A 260 and polyP recovery by digestion with PPX1 and PiBlue assay. Results are plotted as percent of the input amount in each experiment. For all experiments except the Qiaquick kits, the three samples were: (A) DNA alone (5 µg lambda DNA); (B) RNA alone (5 µg polyG); (C) 5 µg polyP alone; (D) a mixture of 5 µg polyP plus 5 µg of DNA; or (E) 5 µg polyP plus 5 µg of RNA (E) . Experiments with the Qiaquick kit employed 2.5 µg of polyP and/or nucleic acid. Data are mean ± SEM ( n = 3).
    Bacteriophage Lambda Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacteriophage lambda dna/product/New England Biolabs
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    93
    Promega lambda phage dna
    Recovery of nucleic acids and polyphosphate (polyP) using various purification methods. Known quantities of bacteriophage <t>lambda</t> <t>DNA</t> (DNA, striped bars), polyG (RNA, white bars), or long-chain polyP (black bars) were processed for nucleic acid purification using Qiaquick, DNeasy, or TRIzol kits, following the manufacturer’s instructions, or with phenol/chloroform extraction. Nucleic acid recovery was quantified using A 260 and polyP recovery by digestion with PPX1 and PiBlue assay. Results are plotted as percent of the input amount in each experiment. For all experiments except the Qiaquick kits, the three samples were: (A) DNA alone (5 µg lambda DNA); (B) RNA alone (5 µg polyG); (C) 5 µg polyP alone; (D) a mixture of 5 µg polyP plus 5 µg of DNA; or (E) 5 µg polyP plus 5 µg of RNA (E) . Experiments with the Qiaquick kit employed 2.5 µg of polyP and/or nucleic acid. Data are mean ± SEM ( n = 3).
    Lambda Phage Dna, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Promega unmethylated lambda phage ci857 sam7 dna
    Recovery of nucleic acids and polyphosphate (polyP) using various purification methods. Known quantities of bacteriophage <t>lambda</t> <t>DNA</t> (DNA, striped bars), polyG (RNA, white bars), or long-chain polyP (black bars) were processed for nucleic acid purification using Qiaquick, DNeasy, or TRIzol kits, following the manufacturer’s instructions, or with phenol/chloroform extraction. Nucleic acid recovery was quantified using A 260 and polyP recovery by digestion with PPX1 and PiBlue assay. Results are plotted as percent of the input amount in each experiment. For all experiments except the Qiaquick kits, the three samples were: (A) DNA alone (5 µg lambda DNA); (B) RNA alone (5 µg polyG); (C) 5 µg polyP alone; (D) a mixture of 5 µg polyP plus 5 µg of DNA; or (E) 5 µg polyP plus 5 µg of RNA (E) . Experiments with the Qiaquick kit employed 2.5 µg of polyP and/or nucleic acid. Data are mean ± SEM ( n = 3).
    Unmethylated Lambda Phage Ci857 Sam7 Dna, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Promega three dimensional nanofunnels lambda phage dna
    Residence time measurements and threshold electric field reduction. a Mean residence times measured at various nanochannel electric field strengths, E , in three different <t>nanofunnels</t> (defined by α = 0.78, α = 0.45, and α = 0; see Eq. 2 ) and in the absence of a nanofunnel. Each dataset is associated with the nanofunnel indicated directly above it in the figure and is color coded accordingly. Data were collected using stained λ-phage (48.5 kbp, open circles) and T4-phage (165.6 kbp, filled squares) <t>DNA</t> molecules. The error bars indicate the standard deviations of at least 20 independent measurements per experimental data point. The curves represent the best-fit of the theoretical model to these experimental data. b Extrapolating the experimental electric field data in ( a ) to τ 0 = 6 ms results in the characteristic threshold electric field strength, E 0 (normalized to the threshold electric field strength measured in the absence of a nanofunnel E 0 (no funnel)) for each of the three nanofunnels (red, green, and blue symbols obtained from the data in ( a ) of the same colors). τ 0 = 6 ms corresponds to the Zimm relaxation time of the leading portion (~3000 bp) of the nanofunnel-confined molecule 23 , 24 . This 3000-bp segment is the length of DNA that must enter the nanochannel to initiate threading, as determined from the theoretical monomer concentration profiles shown in Supplementary Fig. 7b . The experimental values in ( b ) for the α = 1.46 and α = 1.89 nanofunnels were determined as described in the Supplementary Methods. The solid and dashed black lines are interpolations of theoretical values calculated for T4-phage and λ-phage DNA, respectively, over a wider range of α values. The error bars indicate the 1 σ confidence level of the threshold electric field strengths determined from the experimental data and are smaller than the symbols for the results from nanofunnels where α > 0.45
    Three Dimensional Nanofunnels Lambda Phage Dna, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Promega hindiii digested bacteriophage lambda competitor dna
    Residence time measurements and threshold electric field reduction. a Mean residence times measured at various nanochannel electric field strengths, E , in three different <t>nanofunnels</t> (defined by α = 0.78, α = 0.45, and α = 0; see Eq. 2 ) and in the absence of a nanofunnel. Each dataset is associated with the nanofunnel indicated directly above it in the figure and is color coded accordingly. Data were collected using stained λ-phage (48.5 kbp, open circles) and T4-phage (165.6 kbp, filled squares) <t>DNA</t> molecules. The error bars indicate the standard deviations of at least 20 independent measurements per experimental data point. The curves represent the best-fit of the theoretical model to these experimental data. b Extrapolating the experimental electric field data in ( a ) to τ 0 = 6 ms results in the characteristic threshold electric field strength, E 0 (normalized to the threshold electric field strength measured in the absence of a nanofunnel E 0 (no funnel)) for each of the three nanofunnels (red, green, and blue symbols obtained from the data in ( a ) of the same colors). τ 0 = 6 ms corresponds to the Zimm relaxation time of the leading portion (~3000 bp) of the nanofunnel-confined molecule 23 , 24 . This 3000-bp segment is the length of DNA that must enter the nanochannel to initiate threading, as determined from the theoretical monomer concentration profiles shown in Supplementary Fig. 7b . The experimental values in ( b ) for the α = 1.46 and α = 1.89 nanofunnels were determined as described in the Supplementary Methods. The solid and dashed black lines are interpolations of theoretical values calculated for T4-phage and λ-phage DNA, respectively, over a wider range of α values. The error bars indicate the 1 σ confidence level of the threshold electric field strengths determined from the experimental data and are smaller than the symbols for the results from nanofunnels where α > 0.45
    Hindiii Digested Bacteriophage Lambda Competitor Dna, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega lambda dna
    Residence time measurements and threshold electric field reduction. a Mean residence times measured at various nanochannel electric field strengths, E , in three different <t>nanofunnels</t> (defined by α = 0.78, α = 0.45, and α = 0; see Eq. 2 ) and in the absence of a nanofunnel. Each dataset is associated with the nanofunnel indicated directly above it in the figure and is color coded accordingly. Data were collected using stained λ-phage (48.5 kbp, open circles) and T4-phage (165.6 kbp, filled squares) <t>DNA</t> molecules. The error bars indicate the standard deviations of at least 20 independent measurements per experimental data point. The curves represent the best-fit of the theoretical model to these experimental data. b Extrapolating the experimental electric field data in ( a ) to τ 0 = 6 ms results in the characteristic threshold electric field strength, E 0 (normalized to the threshold electric field strength measured in the absence of a nanofunnel E 0 (no funnel)) for each of the three nanofunnels (red, green, and blue symbols obtained from the data in ( a ) of the same colors). τ 0 = 6 ms corresponds to the Zimm relaxation time of the leading portion (~3000 bp) of the nanofunnel-confined molecule 23 , 24 . This 3000-bp segment is the length of DNA that must enter the nanochannel to initiate threading, as determined from the theoretical monomer concentration profiles shown in Supplementary Fig. 7b . The experimental values in ( b ) for the α = 1.46 and α = 1.89 nanofunnels were determined as described in the Supplementary Methods. The solid and dashed black lines are interpolations of theoretical values calculated for T4-phage and λ-phage DNA, respectively, over a wider range of α values. The error bars indicate the 1 σ confidence level of the threshold electric field strengths determined from the experimental data and are smaller than the symbols for the results from nanofunnels where α > 0.45
    Lambda Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega unmethylated lambda dna
    Residence time measurements and threshold electric field reduction. a Mean residence times measured at various nanochannel electric field strengths, E , in three different <t>nanofunnels</t> (defined by α = 0.78, α = 0.45, and α = 0; see Eq. 2 ) and in the absence of a nanofunnel. Each dataset is associated with the nanofunnel indicated directly above it in the figure and is color coded accordingly. Data were collected using stained λ-phage (48.5 kbp, open circles) and T4-phage (165.6 kbp, filled squares) <t>DNA</t> molecules. The error bars indicate the standard deviations of at least 20 independent measurements per experimental data point. The curves represent the best-fit of the theoretical model to these experimental data. b Extrapolating the experimental electric field data in ( a ) to τ 0 = 6 ms results in the characteristic threshold electric field strength, E 0 (normalized to the threshold electric field strength measured in the absence of a nanofunnel E 0 (no funnel)) for each of the three nanofunnels (red, green, and blue symbols obtained from the data in ( a ) of the same colors). τ 0 = 6 ms corresponds to the Zimm relaxation time of the leading portion (~3000 bp) of the nanofunnel-confined molecule 23 , 24 . This 3000-bp segment is the length of DNA that must enter the nanochannel to initiate threading, as determined from the theoretical monomer concentration profiles shown in Supplementary Fig. 7b . The experimental values in ( b ) for the α = 1.46 and α = 1.89 nanofunnels were determined as described in the Supplementary Methods. The solid and dashed black lines are interpolations of theoretical values calculated for T4-phage and λ-phage DNA, respectively, over a wider range of α values. The error bars indicate the 1 σ confidence level of the threshold electric field strengths determined from the experimental data and are smaller than the symbols for the results from nanofunnels where α > 0.45
    Unmethylated Lambda Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 419 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Promega lambda dna purification kit
    Residence time measurements and threshold electric field reduction. a Mean residence times measured at various nanochannel electric field strengths, E , in three different <t>nanofunnels</t> (defined by α = 0.78, α = 0.45, and α = 0; see Eq. 2 ) and in the absence of a nanofunnel. Each dataset is associated with the nanofunnel indicated directly above it in the figure and is color coded accordingly. Data were collected using stained λ-phage (48.5 kbp, open circles) and T4-phage (165.6 kbp, filled squares) <t>DNA</t> molecules. The error bars indicate the standard deviations of at least 20 independent measurements per experimental data point. The curves represent the best-fit of the theoretical model to these experimental data. b Extrapolating the experimental electric field data in ( a ) to τ 0 = 6 ms results in the characteristic threshold electric field strength, E 0 (normalized to the threshold electric field strength measured in the absence of a nanofunnel E 0 (no funnel)) for each of the three nanofunnels (red, green, and blue symbols obtained from the data in ( a ) of the same colors). τ 0 = 6 ms corresponds to the Zimm relaxation time of the leading portion (~3000 bp) of the nanofunnel-confined molecule 23 , 24 . This 3000-bp segment is the length of DNA that must enter the nanochannel to initiate threading, as determined from the theoretical monomer concentration profiles shown in Supplementary Fig. 7b . The experimental values in ( b ) for the α = 1.46 and α = 1.89 nanofunnels were determined as described in the Supplementary Methods. The solid and dashed black lines are interpolations of theoretical values calculated for T4-phage and λ-phage DNA, respectively, over a wider range of α values. The error bars indicate the 1 σ confidence level of the threshold electric field strengths determined from the experimental data and are smaller than the symbols for the results from nanofunnels where α > 0.45
    Lambda Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Recovery of nucleic acids and polyphosphate (polyP) using various purification methods. Known quantities of bacteriophage lambda DNA (DNA, striped bars), polyG (RNA, white bars), or long-chain polyP (black bars) were processed for nucleic acid purification using Qiaquick, DNeasy, or TRIzol kits, following the manufacturer’s instructions, or with phenol/chloroform extraction. Nucleic acid recovery was quantified using A 260 and polyP recovery by digestion with PPX1 and PiBlue assay. Results are plotted as percent of the input amount in each experiment. For all experiments except the Qiaquick kits, the three samples were: (A) DNA alone (5 µg lambda DNA); (B) RNA alone (5 µg polyG); (C) 5 µg polyP alone; (D) a mixture of 5 µg polyP plus 5 µg of DNA; or (E) 5 µg polyP plus 5 µg of RNA (E) . Experiments with the Qiaquick kit employed 2.5 µg of polyP and/or nucleic acid. Data are mean ± SEM ( n = 3).

    Journal: Frontiers in Medicine

    Article Title: Ability of Polyphosphate and Nucleic Acids to Trigger Blood Clotting: Some Observations and Caveats

    doi: 10.3389/fmed.2018.00107

    Figure Lengend Snippet: Recovery of nucleic acids and polyphosphate (polyP) using various purification methods. Known quantities of bacteriophage lambda DNA (DNA, striped bars), polyG (RNA, white bars), or long-chain polyP (black bars) were processed for nucleic acid purification using Qiaquick, DNeasy, or TRIzol kits, following the manufacturer’s instructions, or with phenol/chloroform extraction. Nucleic acid recovery was quantified using A 260 and polyP recovery by digestion with PPX1 and PiBlue assay. Results are plotted as percent of the input amount in each experiment. For all experiments except the Qiaquick kits, the three samples were: (A) DNA alone (5 µg lambda DNA); (B) RNA alone (5 µg polyG); (C) 5 µg polyP alone; (D) a mixture of 5 µg polyP plus 5 µg of DNA; or (E) 5 µg polyP plus 5 µg of RNA (E) . Experiments with the Qiaquick kit employed 2.5 µg of polyP and/or nucleic acid. Data are mean ± SEM ( n = 3).

    Article Snippet: Calf intestinal alkaline phosphatase (CIAP) was from Promega and bacteriophage lambda DNA, and DNA ladder were from New England Biolabs.

    Techniques: Purification, Lambda DNA Preparation, Nucleic Acid Purification

    Procoagulant activities of polyphosphate (polyP), DNA, and RNA. Panels display plasma clot times versus concentration, with horizontal dashed lines showing the mean clot time without activator (±SEM as horizontal dotted lines). Symbols represent the mean, and error bars the SE, of three separate experiments evaluating clot times versus concentration. (A) Clot times with short-chain polyP (74 mer, Δ); medium-chain polyP (385 mer, □); and long-chain polyP (1195 mer, ○). (B) Clot times with HEK 293 cell DNA isolated with phenol/chloroform (□, 3 distinct purifications); NET-derived DNA isolated with phenol/chloroform (■, 3 distinct purifications); or lambda DNA ( ). Note that for comparison purposes, the clotting data for HEK 293 DNA and NET DNA in this panel are replotted from those in Supplemental Figure S2 in Supplementary Material of Smith et al. ( 26 ). (C) Clot times with HEK 293 cell RNA (◆); baker’s yeast transfer RNA (○); or the RNA homopolymers, polyA ( ), polyC ( ), polyI ( ), or polyG ( ). PolyU was also tested, but had no procoagulant activity (data not shown).

    Journal: Frontiers in Medicine

    Article Title: Ability of Polyphosphate and Nucleic Acids to Trigger Blood Clotting: Some Observations and Caveats

    doi: 10.3389/fmed.2018.00107

    Figure Lengend Snippet: Procoagulant activities of polyphosphate (polyP), DNA, and RNA. Panels display plasma clot times versus concentration, with horizontal dashed lines showing the mean clot time without activator (±SEM as horizontal dotted lines). Symbols represent the mean, and error bars the SE, of three separate experiments evaluating clot times versus concentration. (A) Clot times with short-chain polyP (74 mer, Δ); medium-chain polyP (385 mer, □); and long-chain polyP (1195 mer, ○). (B) Clot times with HEK 293 cell DNA isolated with phenol/chloroform (□, 3 distinct purifications); NET-derived DNA isolated with phenol/chloroform (■, 3 distinct purifications); or lambda DNA ( ). Note that for comparison purposes, the clotting data for HEK 293 DNA and NET DNA in this panel are replotted from those in Supplemental Figure S2 in Supplementary Material of Smith et al. ( 26 ). (C) Clot times with HEK 293 cell RNA (◆); baker’s yeast transfer RNA (○); or the RNA homopolymers, polyA ( ), polyC ( ), polyI ( ), or polyG ( ). PolyU was also tested, but had no procoagulant activity (data not shown).

    Article Snippet: Calf intestinal alkaline phosphatase (CIAP) was from Promega and bacteriophage lambda DNA, and DNA ladder were from New England Biolabs.

    Techniques: Concentration Assay, Isolation, Derivative Assay, Lambda DNA Preparation, Coagulation, Activity Assay

    Residence time measurements and threshold electric field reduction. a Mean residence times measured at various nanochannel electric field strengths, E , in three different nanofunnels (defined by α = 0.78, α = 0.45, and α = 0; see Eq. 2 ) and in the absence of a nanofunnel. Each dataset is associated with the nanofunnel indicated directly above it in the figure and is color coded accordingly. Data were collected using stained λ-phage (48.5 kbp, open circles) and T4-phage (165.6 kbp, filled squares) DNA molecules. The error bars indicate the standard deviations of at least 20 independent measurements per experimental data point. The curves represent the best-fit of the theoretical model to these experimental data. b Extrapolating the experimental electric field data in ( a ) to τ 0 = 6 ms results in the characteristic threshold electric field strength, E 0 (normalized to the threshold electric field strength measured in the absence of a nanofunnel E 0 (no funnel)) for each of the three nanofunnels (red, green, and blue symbols obtained from the data in ( a ) of the same colors). τ 0 = 6 ms corresponds to the Zimm relaxation time of the leading portion (~3000 bp) of the nanofunnel-confined molecule 23 , 24 . This 3000-bp segment is the length of DNA that must enter the nanochannel to initiate threading, as determined from the theoretical monomer concentration profiles shown in Supplementary Fig. 7b . The experimental values in ( b ) for the α = 1.46 and α = 1.89 nanofunnels were determined as described in the Supplementary Methods. The solid and dashed black lines are interpolations of theoretical values calculated for T4-phage and λ-phage DNA, respectively, over a wider range of α values. The error bars indicate the 1 σ confidence level of the threshold electric field strengths determined from the experimental data and are smaller than the symbols for the results from nanofunnels where α > 0.45

    Journal: Nature Communications

    Article Title: Enhanced nanochannel translocation and localization of genomic DNA molecules using three-dimensional nanofunnels

    doi: 10.1038/s41467-017-00951-4

    Figure Lengend Snippet: Residence time measurements and threshold electric field reduction. a Mean residence times measured at various nanochannel electric field strengths, E , in three different nanofunnels (defined by α = 0.78, α = 0.45, and α = 0; see Eq. 2 ) and in the absence of a nanofunnel. Each dataset is associated with the nanofunnel indicated directly above it in the figure and is color coded accordingly. Data were collected using stained λ-phage (48.5 kbp, open circles) and T4-phage (165.6 kbp, filled squares) DNA molecules. The error bars indicate the standard deviations of at least 20 independent measurements per experimental data point. The curves represent the best-fit of the theoretical model to these experimental data. b Extrapolating the experimental electric field data in ( a ) to τ 0 = 6 ms results in the characteristic threshold electric field strength, E 0 (normalized to the threshold electric field strength measured in the absence of a nanofunnel E 0 (no funnel)) for each of the three nanofunnels (red, green, and blue symbols obtained from the data in ( a ) of the same colors). τ 0 = 6 ms corresponds to the Zimm relaxation time of the leading portion (~3000 bp) of the nanofunnel-confined molecule 23 , 24 . This 3000-bp segment is the length of DNA that must enter the nanochannel to initiate threading, as determined from the theoretical monomer concentration profiles shown in Supplementary Fig. 7b . The experimental values in ( b ) for the α = 1.46 and α = 1.89 nanofunnels were determined as described in the Supplementary Methods. The solid and dashed black lines are interpolations of theoretical values calculated for T4-phage and λ-phage DNA, respectively, over a wider range of α values. The error bars indicate the 1 σ confidence level of the threshold electric field strengths determined from the experimental data and are smaller than the symbols for the results from nanofunnels where α > 0.45

    Article Snippet: Measuring DNA molecules in three-dimensional nanofunnels Lambda-phage DNA (Promega) or T4-phage DNA (Nippon Gene) in 2X TBE was stained with the intercalating dye, YOYO-1 (Invitrogen), at a base-pair:dye ratio of 5:1.

    Techniques: Staining, Mass Spectrometry, Concentration Assay