lactate dehydrogenase ldh Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher lactate dehydrogenase ldh
    Cytotoxic effect of PLFE1 and its GA-enriched fractions on human lung <t>A549</t> line cells. ( A ) PLFE1 was assayed at different concentrations. ( B ) Cytotoxicity of GA-enriched fractions exhibiting pyocyanin production inhibition. <t>LDH</t> release was determined at 1 h, 3 h, 6 h, and 24 h. DMSO was used as a vehicle control. Data are displayed as the mean ± SEM values of four independent experiments performed in duplicate. Statistics were achieved by a two-tailed t test: ***, P =0.0001 to 0.001; **, P =0.001 to 0.01; *, P =0.01 to 0.05; NS (Not Significant) , P ≥0.05.
    Lactate Dehydrogenase Ldh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 338 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactate dehydrogenase ldh/product/Thermo Fisher
    Average 99 stars, based on 338 article reviews
    Price from $9.99 to $1999.99
    lactate dehydrogenase ldh - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    97
    Millipore lactate dehydrogenase ldh
    The effect of graphene oxide (GO) and bacterially reduced graphene oxide (B-rGO) on lactate dehydrogenase <t>(LDH)</t> activity in <t>MCF-7</t> cells. Notes: LDH activity was measured by changes in optical densities due to nicotinamide adenine dinucleotide reduction, monitored at 490 nm, as described in “Materials and methods,” using a cytotoxicity detection lactate dehydrogenase kit. The results represent the means of three separate experiments and the error bars represent the standard error of the mean. Treated groups showed statistically significant differences from the control group, as determined by Student’s t -test ( P
    Lactate Dehydrogenase Ldh, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 710 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactate dehydrogenase ldh/product/Millipore
    Average 97 stars, based on 710 article reviews
    Price from $9.99 to $1999.99
    lactate dehydrogenase ldh - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    99
    Millipore ldh activity assay kit
    Reduction in contrast-induced renal tubule epithelial cell death by PACAP. (A) Incubation of HK-2 cells with <t>Urografin</t> caused a large increase in the release of <t>LDH</t> into the culture medium. PACAP38 and VIP inhibited the increased release of LDH in a dose-dependent manner. PACAP38 was more potent than VIP. (B) Incubation of HK-2 cells with Urografin caused a very large increase in apoptosis. PACAP38 inhibited the increased apoptosis of HK-2 cells in a dose-dependent manner. * P
    Ldh Activity Assay Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ldh activity assay kit/product/Millipore
    Average 99 stars, based on 280 article reviews
    Price from $9.99 to $1999.99
    ldh activity assay kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    97
    Abcam ldh assay kit
    Apigenin decreases the oxidative stress in MI/R-induced H9C2 cells. H9C2 cells were treated with MI/R, and 1, 6 and 25 µM apigenin + MI/R. The levels of (A) <t>LDH,</t> (B) MDA, (C) <t>CAT</t> (D) and ATPase in H9C2 cells was determined using commercial kits. *P
    Ldh Assay Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ldh assay kit/product/Abcam
    Average 97 stars, based on 204 article reviews
    Price from $9.99 to $1999.99
    ldh assay kit - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    93
    Promega lactate dehydrogenase ldh assay
    Quercetin protects against cholesterol-induced decrease in Min6 cell viability . Cell viability was assessed through (A) changes in cellular <t>MTS</t> reduction and (B) release of <t>LDH</t> into the media. Cells were incubated for 6 h with 320 µM cholesterol and/or 10–100 µM QUE. MTS reduction was measured in attached cells and LDH in the supernatant. Values are expressed as mean ± SEM, from three independent culture preparations, each treatment performed in quadruplicate. All one-way ANOVA (p values specified in results), symbols indicate Bonferroni post-test significances # in comparison to control and * to cholesterol-treated Min6 cells.
    Lactate Dehydrogenase Ldh Assay, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactate dehydrogenase ldh assay/product/Promega
    Average 93 stars, based on 309 article reviews
    Price from $9.99 to $1999.99
    lactate dehydrogenase ldh assay - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    99
    TaKaRa lactate dehydrogenase ldh cytotoxicity detection kit
    Lactate dehydrogenase <t>(LDH)</t> and PrestoBlue assays to determine <t>silvestrol-mediated</t> cytotoxicity and cytostaticity. ( A , B ) Processed data for LDH-activity in cell-culture supernatants derived from cells treated with 2 or 50 nM silvestrol as compared to respective DMSO controls in ( A ) A549 cells persistently infected with HEV strain 48932c or ( B ) in non-infected A549/D3. ( C , D ) Processed data for redox-metabolic activity in cells treated with 2 or 50 nM silvestrol when compared to respective DMSO controls in ( C ) A549 cells persistently infected with HEV strain 47832c or ( D ) in non-infected A549/D3 cells. * p
    Lactate Dehydrogenase Ldh Cytotoxicity Detection Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactate dehydrogenase ldh cytotoxicity detection kit/product/TaKaRa
    Average 99 stars, based on 294 article reviews
    Price from $9.99 to $1999.99
    lactate dehydrogenase ldh cytotoxicity detection kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    91
    Beyotime lactate dehydrogenase ldh cytotoxicity assay kit
    Cytotoxicity of <t>MWCNT</t> in MeT-5A cells. MeT-5A cells were seeded in 96-well plate (1 × 10 4 cells/well) and subjected to various treatments. Cell viability was determined by the <t>LDH</t> assay and was expressed as the percentage of the control which was without treatment. Each experiment was repeated at least three times, and error bar stands for standard deviation (SD). (a) MeT-5A cells were treated with MWCNT at different concentrations (0, 1.25, 2.5, 5, 10, 20, and 40 μ g/cm 2 ) for 24 h. (b) MeT-5A cells were treated with MWCNT for different times (0, 24, 48, and 72 h) at 10 μ g/cm 2 . ∗ P
    Lactate Dehydrogenase Ldh Cytotoxicity Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 91/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactate dehydrogenase ldh cytotoxicity assay kit/product/Beyotime
    Average 91 stars, based on 221 article reviews
    Price from $9.99 to $1999.99
    lactate dehydrogenase ldh cytotoxicity assay kit - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    92
    Promega lactate dehydrogenase ldh release assay
    <t>HPE</t> does not impair RAW 264.7 cell viability. (A) RAW 264.7 cell viability was measured by <t>LDH</t> release assay after 4 h of treatment with 1.25 mg/mL HPE or volume equivalent of vehicle in the presence or absence of L. pneumophila Δ flaA infection (MOI of 1), as indicated. Lysis solution was used as a control for 100% cytotoxicity. (B) RAW 264.7 cells were treated with 1.25 mg/mL HPE or volume equivalent of vehicle in the presence or absence of L. pneumophila Δ flaA infection (MOI of 1), as indicated, and abundance of full-length caspase 3 was visualized by Western blotting (top panel) and quantified by densitometric analysis relative to the β-actin loading control (bottom panel). As a control for apoptosis, cells were treated with staurosporine (STS) for 3 h. Data shown are representative of two independent experiments.
    Lactate Dehydrogenase Ldh Release Assay, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactate dehydrogenase ldh release assay/product/Promega
    Average 92 stars, based on 461 article reviews
    Price from $9.99 to $1999.99
    lactate dehydrogenase ldh release assay - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    Abcam ldh activity
    Effect of E4 in combination with different receptor inhibitors on <t>LDH</t> activity in primary hippocampal neuronal cultures subjected to the H 2 O 2 -induced oxidative stress. Primary hippocampal cell cultures were exposed to 3.25 mM E4 alone or in combination with <t>MPP,</t> PHTTP, G15 and/or 2-BR after induction of oxidative stress. (A) LDH activity was significantly decreased by treatment with E4 alone or in combination with ER-α inhibitor MPP compared to the H 2 O 2 -treated cell cultures or cultures combinedly treated by E4 + MPP + PHTTP. Combined use of MPP and PHTTP significantly increased the LDH activity compared to the cells treated by E4 alone or in combination with MPP. (B) LDH activity was significantly decreased by treatment with E4 alone or in combination with ER-β inhibitor PHTTP compared to the H 2 O 2 -treated cell cultures or cultures combinedly treated by E4 + MPP + PHTTP. Combined use of MPP and PHTTP significantly increased the LDH compared to the cell cultures treated by E4 alone or in combination with PHTTP. (C) Inhibition of palmitoylation alone or in combination with MPP significantly downregulated LDH activity compared to the H 2 O 2 -treated cells or to those treated by E4 alone. Combination of E4 with 2-BR, MPP and PHTTP significantly upregulated LDH activity compared to the cell cultures treated by E4 or 2-BR alone or in combination with MPP. (D) Cell cultures treated by E4 alone or in combination with GPR30 inhibitor G15 had significantly lower LDH activity compared to the cultures treated by H 2 O 2 alone. No significant difference was observed between the cells treated by E4 alone or in combination with G15.
    Ldh Activity, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ldh activity/product/Abcam
    Average 99 stars, based on 156 article reviews
    Price from $9.99 to $1999.99
    ldh activity - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    93
    Olympus lactate dehydrogenase ldh
    Correlations between serum <t>LDL/HDL</t> ratio and PaO 2 (A) or <t>LDH</t> (B) . Serum LDL-C/HDL-C ratios correlated negatively with PaO 2 levels (r = -0.403, p = 0.027) and positively with LDH (r = 0.381, p = 0.034), n = 33.
    Lactate Dehydrogenase Ldh, supplied by Olympus, used in various techniques. Bioz Stars score: 93/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactate dehydrogenase ldh/product/Olympus
    Average 93 stars, based on 104 article reviews
    Price from $9.99 to $1999.99
    lactate dehydrogenase ldh - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    93
    Pointe Scientific lactate dehydrogenase ldh
    Effect of anti-OPN Ab treatment on plasma levels of organ injury markers and proinflammatory cytokines and chemokine in CLP animals . Sepsis was induced in mice by CLP and anti-OPN Ab or non-immunized IgG control at a dose of 50 μg/mice in 100 μl volumes was injected through the jugular vein. In the vehicle group, 100 μl of PBS was injected in CLP mice via the jugular vein. Blood samples were drawn by cardiac puncture at 20 h of sham-operated, vehicle and anti-OPN Ab-treated mice for measuring (A) ALT, (B) <t>AST</t> and (C) <t>LDH.</t> Similarly, the blood samples collected at 20 h after CLP were measured for (D) IL-6, (E) IL-1β and (F) MIP-2 by ELISA. Data are expressed as means ± SEM (n = 5 mice/group) and compared by one-way ANOVA and SNK method ( * P
    Lactate Dehydrogenase Ldh, supplied by Pointe Scientific, used in various techniques. Bioz Stars score: 93/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactate dehydrogenase ldh/product/Pointe Scientific
    Average 93 stars, based on 97 article reviews
    Price from $9.99 to $1999.99
    lactate dehydrogenase ldh - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    99
    Thermo Fisher lactate dehydrogenase ldh cytotoxicity assay kit
    Cytotoxic effect of carbapenem-susceptible and carbapenem-resistant A . baumannii strains on <t>HaCaT</t> keratinocyte cells. Cytotoxic levels caused by carbapenem-susceptible strain 813 and carbapenem-resistant strains 1995/12 and 4031 on HaCaT cells were measured by <t>LDH</t> assay. Values are the means from results obtained in triplicate. Error bars represent the standard deviation of the mean value.
    Lactate Dehydrogenase Ldh Cytotoxicity Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactate dehydrogenase ldh cytotoxicity assay kit/product/Thermo Fisher
    Average 99 stars, based on 110 article reviews
    Price from $9.99 to $1999.99
    lactate dehydrogenase ldh cytotoxicity assay kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher lactate dehydrogenase ldh assay
    Cytotoxic effect of carbapenem-susceptible and carbapenem-resistant A . baumannii strains on <t>HaCaT</t> keratinocyte cells. Cytotoxic levels caused by carbapenem-susceptible strain 813 and carbapenem-resistant strains 1995/12 and 4031 on HaCaT cells were measured by <t>LDH</t> assay. Values are the means from results obtained in triplicate. Error bars represent the standard deviation of the mean value.
    Lactate Dehydrogenase Ldh Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactate dehydrogenase ldh assay/product/Thermo Fisher
    Average 99 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    lactate dehydrogenase ldh assay - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    93
    Cayman Chemical lactate dehydrogenase ldh
    <t>LDH</t> activities of cultured human blood cells treated with 10, 25, 50, 75, 100, 150 and 200 mg/L of <t>TPO</t> for 24 and 48 h (Control: whole blood without terpinolene. Each value represents mean ± SD of five experiments;
    Lactate Dehydrogenase Ldh, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 93/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lactate dehydrogenase ldh/product/Cayman Chemical
    Average 93 stars, based on 158 article reviews
    Price from $9.99 to $1999.99
    lactate dehydrogenase ldh - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Cytotoxic effect of PLFE1 and its GA-enriched fractions on human lung A549 line cells. ( A ) PLFE1 was assayed at different concentrations. ( B ) Cytotoxicity of GA-enriched fractions exhibiting pyocyanin production inhibition. LDH release was determined at 1 h, 3 h, 6 h, and 24 h. DMSO was used as a vehicle control. Data are displayed as the mean ± SEM values of four independent experiments performed in duplicate. Statistics were achieved by a two-tailed t test: ***, P =0.0001 to 0.001; **, P =0.001 to 0.01; *, P =0.01 to 0.05; NS (Not Significant) , P ≥0.05.

    Journal: bioRxiv

    Article Title: Membrane-Interactive Compounds from Pistacia lentiscus L. Thwart Pseudomonas aeruginosa Virulence

    doi: 10.1101/2020.03.17.995043

    Figure Lengend Snippet: Cytotoxic effect of PLFE1 and its GA-enriched fractions on human lung A549 line cells. ( A ) PLFE1 was assayed at different concentrations. ( B ) Cytotoxicity of GA-enriched fractions exhibiting pyocyanin production inhibition. LDH release was determined at 1 h, 3 h, 6 h, and 24 h. DMSO was used as a vehicle control. Data are displayed as the mean ± SEM values of four independent experiments performed in duplicate. Statistics were achieved by a two-tailed t test: ***, P =0.0001 to 0.001; **, P =0.001 to 0.01; *, P =0.01 to 0.05; NS (Not Significant) , P ≥0.05.

    Article Snippet: The anti-virulence effect of PLFE1 or ginkgolic acid-enriched fractions (PLFE1-(2-4)) on P. aeruginosa H103 was determined using an enzymatic assay (Pierce™ LDH Cytotoxicity Assay Kit, Thermo Scientific™), which measures lactate dehydrogenase (LDH) released from the cytosol of damaged A549 cells into the supernatant.

    Techniques: Inhibition, Two Tailed Test

    MiR-212 ameliorates MPP + -induced damage of SH-SY5Y cells. SH-SY5Y cells transfected with miR-212 mimics or anti-miR-212 were treated with or without 2 mM MPP + for 24 h, followed by ELISA assays of caspase-3 activity (A), LDH release (B), SOD level (C), ROS production (D), TNF-α expression (E), and IL-1β secretion (F). * p

    Journal: Yonsei Medical Journal

    Article Title: MiR-212 Attenuates MPP+-Induced Neuronal Damage by Targeting KLF4 in SH-SY5Y Cells

    doi: 10.3349/ymj.2018.59.3.416

    Figure Lengend Snippet: MiR-212 ameliorates MPP + -induced damage of SH-SY5Y cells. SH-SY5Y cells transfected with miR-212 mimics or anti-miR-212 were treated with or without 2 mM MPP + for 24 h, followed by ELISA assays of caspase-3 activity (A), LDH release (B), SOD level (C), ROS production (D), TNF-α expression (E), and IL-1β secretion (F). * p

    Article Snippet: Additionally, lactate dehydrogenase (LDH) release, superoxide dismutase (SOD) level, reactive oxygen species (ROS) production, and tumor necrosis factor-α (TNF-α), and interleukin-1 beta (IL-1β) expression were measured using corresponding commercially available ELISA kits (Invitrogen), according to the provided instructions.

    Techniques: Transfection, Enzyme-linked Immunosorbent Assay, Activity Assay, Expressing

    The effect of graphene oxide (GO) and bacterially reduced graphene oxide (B-rGO) on lactate dehydrogenase (LDH) activity in MCF-7 cells. Notes: LDH activity was measured by changes in optical densities due to nicotinamide adenine dinucleotide reduction, monitored at 490 nm, as described in “Materials and methods,” using a cytotoxicity detection lactate dehydrogenase kit. The results represent the means of three separate experiments and the error bars represent the standard error of the mean. Treated groups showed statistically significant differences from the control group, as determined by Student’s t -test ( P

    Journal: International Journal of Nanomedicine

    Article Title: Green synthesis of graphene and its cytotoxic effects in human breast cancer cells

    doi: 10.2147/IJN.S42047

    Figure Lengend Snippet: The effect of graphene oxide (GO) and bacterially reduced graphene oxide (B-rGO) on lactate dehydrogenase (LDH) activity in MCF-7 cells. Notes: LDH activity was measured by changes in optical densities due to nicotinamide adenine dinucleotide reduction, monitored at 490 nm, as described in “Materials and methods,” using a cytotoxicity detection lactate dehydrogenase kit. The results represent the means of three separate experiments and the error bars represent the standard error of the mean. Treated groups showed statistically significant differences from the control group, as determined by Student’s t -test ( P

    Article Snippet: Membrane integrity The cell membrane integrity of MCF-7 cells was evaluated by determining the activity of lactate dehydrogenase (LDH) leaking out of the cells using an in vitro toxicology assay kit (TOX7, Sigma-Aldrich) according to the manufacturer’s instructions.

    Techniques: Activity Assay

    Reduction in contrast-induced renal tubule epithelial cell death by PACAP. (A) Incubation of HK-2 cells with Urografin caused a large increase in the release of LDH into the culture medium. PACAP38 and VIP inhibited the increased release of LDH in a dose-dependent manner. PACAP38 was more potent than VIP. (B) Incubation of HK-2 cells with Urografin caused a very large increase in apoptosis. PACAP38 inhibited the increased apoptosis of HK-2 cells in a dose-dependent manner. * P

    Journal: Physiological Reports

    Article Title: Pituitary adenylate cyclase-activating polypeptide prevents contrast-induced nephropathy in a novel mouse model

    doi: 10.1002/phy2.163

    Figure Lengend Snippet: Reduction in contrast-induced renal tubule epithelial cell death by PACAP. (A) Incubation of HK-2 cells with Urografin caused a large increase in the release of LDH into the culture medium. PACAP38 and VIP inhibited the increased release of LDH in a dose-dependent manner. PACAP38 was more potent than VIP. (B) Incubation of HK-2 cells with Urografin caused a very large increase in apoptosis. PACAP38 inhibited the increased apoptosis of HK-2 cells in a dose-dependent manner. * P

    Article Snippet: To determine the dose–response curves for inhibition of contrast-induced apoptosis, HK-2 cells were treated with 10−9 –10−6 mol/L PACAP or VIP for 1 h prior to exposing them to Urografin at 50 mg I/mL for 24 h. The effects of 10−9 –10−6 mol/L PACAP38 or VIP on the release of the cytoplasmic enzyme lactate dehydrogenase (LDH) into the HK-2 cell culture medium after 24 h of exposure to Urografin (50 mg I/mL) were determined with an LDH activity assay kit (Sigma, St. Louis, MO) according to the manufacturer's instructions.

    Techniques: Incubation

    Neuroprotective effects of various extracts obtained from green and roasted coffee beans on H 2 O 2 -induced cytotoxicity in PC12 cells. (A) Levels of cell viability were measured using the MTT assay. (B) LDH activity in culture supernatants was measured with a colorimetric LDH assay kit. Results shown are means±SD (n=3). Different small letters indicate significant differences.

    Journal: Preventive Nutrition and Food Science

    Article Title: Antioxidant and Neuronal Cell Protective Effects of Columbia Arabica Coffee with Different Roasting Conditions

    doi: 10.3746/pnf.2013.18.1.030

    Figure Lengend Snippet: Neuroprotective effects of various extracts obtained from green and roasted coffee beans on H 2 O 2 -induced cytotoxicity in PC12 cells. (A) Levels of cell viability were measured using the MTT assay. (B) LDH activity in culture supernatants was measured with a colorimetric LDH assay kit. Results shown are means±SD (n=3). Different small letters indicate significant differences.

    Article Snippet: Materials Folin-Ciocalteu’s phenol reagent, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS), potassium persulfate, 2,4,6-tripyridyl-S-triazine (TPTZ), trichloroacetic acid (TCA), thiobarbituric acid (TBA), vitamin C, α-tocopherol, catechin, 2-[4-(2-hydroxyethyl) piperazin-1-yl]ethanesulfonic acid (HEPES), sodium bicarbonate, penicillin, streptomycin, 5-O-caffeoylquinic acid, syringic acid, epigallocatechin gallate, ferrous sulfate (FeSO4 ), hydrogen peroxide (H2 O2 ), dimethyl sulfoxide (DMSO), 3-[4,5-dimethythiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay kit, lactate dehydrogenase (LDH) assay kit and all solvents used were of analytical grade and purchased from Sigma Chemical Co. (St. Louis, MO, USA).

    Techniques: MTT Assay, Activity Assay, Lactate Dehydrogenase Assay

    Apigenin decreases the oxidative stress in MI/R-induced H9C2 cells. H9C2 cells were treated with MI/R, and 1, 6 and 25 µM apigenin + MI/R. The levels of (A) LDH, (B) MDA, (C) CAT (D) and ATPase in H9C2 cells was determined using commercial kits. *P

    Journal: Molecular Medicine Reports

    Article Title: Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia-reperfusion injury via upregulation of the PI3K/Akt pathway

    doi: 10.3892/mmr.2018.9115

    Figure Lengend Snippet: Apigenin decreases the oxidative stress in MI/R-induced H9C2 cells. H9C2 cells were treated with MI/R, and 1, 6 and 25 µM apigenin + MI/R. The levels of (A) LDH, (B) MDA, (C) CAT (D) and ATPase in H9C2 cells was determined using commercial kits. *P

    Article Snippet: Cells were subsequently collected and the content/activity of lactate dehydrogenase (LDH), malondialdehyde (MDA), catalase (CAT), Na+ K+ -ATPase and Ca2+ -ATPase were determined using a LDH Assay Kit (cat. no. ab102526), MDA Assay Kit (cat. no. ab118970) (both from Abcam, Cambridge, UK), CAT Assay Kit (cat. no. BC0205), Na+ K+ -ATPase assay kit (cat. no. BC0065) and Ca2+ -ATPase assay kit (cat. no. BC0960) (all from Beijing Solarbio Science & Technology Co., Ltd.), respectively, in accordance with the manufacturers' protocol.

    Techniques: Multiple Displacement Amplification

    miR-125b-5p exerts inhibitory effects on hypoxia/reoxygenation-induced apoptosis of cardiomyocytes by suppressing NLRC5 expression. (A) The expression of NLRC5 and miR-125b-5p in hypoxia/reoxygenation-treated HL-1 cells were determined by reverse transcription-quantitative PCR after transfection of the cells with the vectors or mimics. (B) Levels of LDH and MDA in the culture supernatant were measured by assay kits. (C) The fluorescence-activated cell sorting analysis of cardiomyocyte apoptosis following transfection with NLRC5 and miR-125b-5p. The data are presented as the mean ± standard deviation (n=3). $$$ P

    Journal: Experimental and Therapeutic Medicine

    Article Title: LncRNA MALAT1 prevents the protective effects of miR-125b-5p against acute myocardial infarction through positive regulation of NLRC5

    doi: 10.3892/etm.2019.8309

    Figure Lengend Snippet: miR-125b-5p exerts inhibitory effects on hypoxia/reoxygenation-induced apoptosis of cardiomyocytes by suppressing NLRC5 expression. (A) The expression of NLRC5 and miR-125b-5p in hypoxia/reoxygenation-treated HL-1 cells were determined by reverse transcription-quantitative PCR after transfection of the cells with the vectors or mimics. (B) Levels of LDH and MDA in the culture supernatant were measured by assay kits. (C) The fluorescence-activated cell sorting analysis of cardiomyocyte apoptosis following transfection with NLRC5 and miR-125b-5p. The data are presented as the mean ± standard deviation (n=3). $$$ P

    Article Snippet: Measurement of L-lactate dehydrogenase (LDH) and malondialdehyde (MDA) The LDH level in the supernatant was quantified using an LDH assay kit (ab102526, Abcam) according to the manufacturer's instructions.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Transfection, Multiple Displacement Amplification, Measured Assay, Fluorescence, FACS, Standard Deviation

    MALAT1 alleviates the protective effect of miR-125b-5p on hypoxia/reoxygenation-induced apoptosis of cardiomyocytes by upregulation of NLRC5. (A) mRNA levels of MALAT-1 in transfected and control cells were confirmed. (B) The expression of NLRC5 in transfected cell was confirmed by western blotting. (C) Levels of LDH and MDA in the culture supernatant were quantified using assay kits. (D) The fluorescence-activated cell sorting analysis of cardiomyocyte apoptosis in transfected HL-1 cells. The data are presented as the mean ± standard deviation (n=3). $$$ P

    Journal: Experimental and Therapeutic Medicine

    Article Title: LncRNA MALAT1 prevents the protective effects of miR-125b-5p against acute myocardial infarction through positive regulation of NLRC5

    doi: 10.3892/etm.2019.8309

    Figure Lengend Snippet: MALAT1 alleviates the protective effect of miR-125b-5p on hypoxia/reoxygenation-induced apoptosis of cardiomyocytes by upregulation of NLRC5. (A) mRNA levels of MALAT-1 in transfected and control cells were confirmed. (B) The expression of NLRC5 in transfected cell was confirmed by western blotting. (C) Levels of LDH and MDA in the culture supernatant were quantified using assay kits. (D) The fluorescence-activated cell sorting analysis of cardiomyocyte apoptosis in transfected HL-1 cells. The data are presented as the mean ± standard deviation (n=3). $$$ P

    Article Snippet: Measurement of L-lactate dehydrogenase (LDH) and malondialdehyde (MDA) The LDH level in the supernatant was quantified using an LDH assay kit (ab102526, Abcam) according to the manufacturer's instructions.

    Techniques: Transfection, Expressing, Western Blot, Multiple Displacement Amplification, Fluorescence, FACS, Standard Deviation

    Quercetin protects against cholesterol-induced decrease in Min6 cell viability . Cell viability was assessed through (A) changes in cellular MTS reduction and (B) release of LDH into the media. Cells were incubated for 6 h with 320 µM cholesterol and/or 10–100 µM QUE. MTS reduction was measured in attached cells and LDH in the supernatant. Values are expressed as mean ± SEM, from three independent culture preparations, each treatment performed in quadruplicate. All one-way ANOVA (p values specified in results), symbols indicate Bonferroni post-test significances # in comparison to control and * to cholesterol-treated Min6 cells.

    Journal: Redox Biology

    Article Title: The deleterious effect of cholesterol and protection by quercetin on mitochondrial bioenergetics of pancreatic β-cells, glycemic control and inflammation: In vitro and in vivo studies

    doi: 10.1016/j.redox.2016.08.007

    Figure Lengend Snippet: Quercetin protects against cholesterol-induced decrease in Min6 cell viability . Cell viability was assessed through (A) changes in cellular MTS reduction and (B) release of LDH into the media. Cells were incubated for 6 h with 320 µM cholesterol and/or 10–100 µM QUE. MTS reduction was measured in attached cells and LDH in the supernatant. Values are expressed as mean ± SEM, from three independent culture preparations, each treatment performed in quadruplicate. All one-way ANOVA (p values specified in results), symbols indicate Bonferroni post-test significances # in comparison to control and * to cholesterol-treated Min6 cells.

    Article Snippet: 2.5 Cell viability quantification Cell viability was assessed with the MTS assay (CellTiter 96® AQueous assay,) and by lactate dehydrogenase (LDH) release (CytoTox-ONE™ assay, both from Promega, WI, USA) in 96-well plates .

    Techniques: Incubation

    CTL cytotoxicity measured by the LDH assay (n=3). CTLs were generated as described in Methods from the spleens harvested from mice subjected to PBS, avasimibe, CSC-DC vaccine, or CSC-DC vaccine combined with avasimibe. Target cells were ALDH + CSC SCC7. Data were analyzed by t test. Error bars denote SEM; * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Synergy of Dendritic Cell Vaccines and Avasimibe in Treatment of Head and Neck Cancer in Mice

    doi: 10.12659/MSM.905814

    Figure Lengend Snippet: CTL cytotoxicity measured by the LDH assay (n=3). CTLs were generated as described in Methods from the spleens harvested from mice subjected to PBS, avasimibe, CSC-DC vaccine, or CSC-DC vaccine combined with avasimibe. Target cells were ALDH + CSC SCC7. Data were analyzed by t test. Error bars denote SEM; * P

    Article Snippet: CTL-mediated CSC cytotoxicity was tested using the lactate dehydrogenase (LDH) Release Assay (CytoTox 96 Non-Radioactive Cytotoxicity Assay, Promega) according to the manufacturer’s protocol.

    Techniques: CTL Assay, Lactate Dehydrogenase Assay, Generated, Mouse Assay

    Lactate dehydrogenase (LDH) and PrestoBlue assays to determine silvestrol-mediated cytotoxicity and cytostaticity. ( A , B ) Processed data for LDH-activity in cell-culture supernatants derived from cells treated with 2 or 50 nM silvestrol as compared to respective DMSO controls in ( A ) A549 cells persistently infected with HEV strain 48932c or ( B ) in non-infected A549/D3. ( C , D ) Processed data for redox-metabolic activity in cells treated with 2 or 50 nM silvestrol when compared to respective DMSO controls in ( C ) A549 cells persistently infected with HEV strain 47832c or ( D ) in non-infected A549/D3 cells. * p

    Journal: Viruses

    Article Title: Inhibition of Hepatitis E Virus Spread by the Natural Compound Silvestrol

    doi: 10.3390/v10060301

    Figure Lengend Snippet: Lactate dehydrogenase (LDH) and PrestoBlue assays to determine silvestrol-mediated cytotoxicity and cytostaticity. ( A , B ) Processed data for LDH-activity in cell-culture supernatants derived from cells treated with 2 or 50 nM silvestrol as compared to respective DMSO controls in ( A ) A549 cells persistently infected with HEV strain 48932c or ( B ) in non-infected A549/D3. ( C , D ) Processed data for redox-metabolic activity in cells treated with 2 or 50 nM silvestrol when compared to respective DMSO controls in ( C ) A549 cells persistently infected with HEV strain 47832c or ( D ) in non-infected A549/D3 cells. * p

    Article Snippet: Viability was monitored 24 h, 48 h, and 72 h after the beginning of the silvestrol treatment using the lactate dehydrogenase (LDH) Cytotoxicity Detection Kit (Clontech, Mountain View, CA, USA) and the PrestoBlue® Cell Viability Reagent (Life Technologies, Carlsbad, CA, USA), according to the manufacturer’s protocol.

    Techniques: Activity Assay, Cell Culture, Derivative Assay, Infection

    Cytotoxicity of MWCNT in MeT-5A cells. MeT-5A cells were seeded in 96-well plate (1 × 10 4 cells/well) and subjected to various treatments. Cell viability was determined by the LDH assay and was expressed as the percentage of the control which was without treatment. Each experiment was repeated at least three times, and error bar stands for standard deviation (SD). (a) MeT-5A cells were treated with MWCNT at different concentrations (0, 1.25, 2.5, 5, 10, 20, and 40 μ g/cm 2 ) for 24 h. (b) MeT-5A cells were treated with MWCNT for different times (0, 24, 48, and 72 h) at 10 μ g/cm 2 . ∗ P

    Journal: BioMed Research International

    Article Title: Different Cellular Response of Human Mesothelial Cell MeT-5A to Short-Term and Long-Term Multiwalled Carbon Nanotubes Exposure

    doi: 10.1155/2017/2747215

    Figure Lengend Snippet: Cytotoxicity of MWCNT in MeT-5A cells. MeT-5A cells were seeded in 96-well plate (1 × 10 4 cells/well) and subjected to various treatments. Cell viability was determined by the LDH assay and was expressed as the percentage of the control which was without treatment. Each experiment was repeated at least three times, and error bar stands for standard deviation (SD). (a) MeT-5A cells were treated with MWCNT at different concentrations (0, 1.25, 2.5, 5, 10, 20, and 40 μ g/cm 2 ) for 24 h. (b) MeT-5A cells were treated with MWCNT for different times (0, 24, 48, and 72 h) at 10 μ g/cm 2 . ∗ P

    Article Snippet: The short-term effects of MWCNT on cell viability were examined by lactate dehydrogenase (LDH) cytotoxicity assay kit (Beyotime, Shanghai, China) according to the manufacturer's instruction.

    Techniques: Lactate Dehydrogenase Assay, Standard Deviation

    HPE does not impair RAW 264.7 cell viability. (A) RAW 264.7 cell viability was measured by LDH release assay after 4 h of treatment with 1.25 mg/mL HPE or volume equivalent of vehicle in the presence or absence of L. pneumophila Δ flaA infection (MOI of 1), as indicated. Lysis solution was used as a control for 100% cytotoxicity. (B) RAW 264.7 cells were treated with 1.25 mg/mL HPE or volume equivalent of vehicle in the presence or absence of L. pneumophila Δ flaA infection (MOI of 1), as indicated, and abundance of full-length caspase 3 was visualized by Western blotting (top panel) and quantified by densitometric analysis relative to the β-actin loading control (bottom panel). As a control for apoptosis, cells were treated with staurosporine (STS) for 3 h. Data shown are representative of two independent experiments.

    Journal: bioRxiv

    Article Title: High-polyphenol extracts from Sorghum bicolor attenuate replication of Legionella pneumophila within RAW 264.7 macrophages

    doi: 10.1101/2019.12.19.883249

    Figure Lengend Snippet: HPE does not impair RAW 264.7 cell viability. (A) RAW 264.7 cell viability was measured by LDH release assay after 4 h of treatment with 1.25 mg/mL HPE or volume equivalent of vehicle in the presence or absence of L. pneumophila Δ flaA infection (MOI of 1), as indicated. Lysis solution was used as a control for 100% cytotoxicity. (B) RAW 264.7 cells were treated with 1.25 mg/mL HPE or volume equivalent of vehicle in the presence or absence of L. pneumophila Δ flaA infection (MOI of 1), as indicated, and abundance of full-length caspase 3 was visualized by Western blotting (top panel) and quantified by densitometric analysis relative to the β-actin loading control (bottom panel). As a control for apoptosis, cells were treated with staurosporine (STS) for 3 h. Data shown are representative of two independent experiments.

    Article Snippet: To determine whether HPE was toxic to RAW 264.7 cells, we utilized a lactate dehydrogenase (LDH) release assay, which is used to quantify cell lysis.

    Techniques: Lactate Dehydrogenase Assay, Infection, Lysis, Western Blot

    Effect of E4 in combination with different receptor inhibitors on LDH activity in primary hippocampal neuronal cultures subjected to the H 2 O 2 -induced oxidative stress. Primary hippocampal cell cultures were exposed to 3.25 mM E4 alone or in combination with MPP, PHTTP, G15 and/or 2-BR after induction of oxidative stress. (A) LDH activity was significantly decreased by treatment with E4 alone or in combination with ER-α inhibitor MPP compared to the H 2 O 2 -treated cell cultures or cultures combinedly treated by E4 + MPP + PHTTP. Combined use of MPP and PHTTP significantly increased the LDH activity compared to the cells treated by E4 alone or in combination with MPP. (B) LDH activity was significantly decreased by treatment with E4 alone or in combination with ER-β inhibitor PHTTP compared to the H 2 O 2 -treated cell cultures or cultures combinedly treated by E4 + MPP + PHTTP. Combined use of MPP and PHTTP significantly increased the LDH compared to the cell cultures treated by E4 alone or in combination with PHTTP. (C) Inhibition of palmitoylation alone or in combination with MPP significantly downregulated LDH activity compared to the H 2 O 2 -treated cells or to those treated by E4 alone. Combination of E4 with 2-BR, MPP and PHTTP significantly upregulated LDH activity compared to the cell cultures treated by E4 or 2-BR alone or in combination with MPP. (D) Cell cultures treated by E4 alone or in combination with GPR30 inhibitor G15 had significantly lower LDH activity compared to the cultures treated by H 2 O 2 alone. No significant difference was observed between the cells treated by E4 alone or in combination with G15.

    Journal: The Journal of Endocrinology

    Article Title: Estrogen receptors and estetrol-dependent neuroprotective actions: a pilot study

    doi: 10.1530/JOE-16-0434

    Figure Lengend Snippet: Effect of E4 in combination with different receptor inhibitors on LDH activity in primary hippocampal neuronal cultures subjected to the H 2 O 2 -induced oxidative stress. Primary hippocampal cell cultures were exposed to 3.25 mM E4 alone or in combination with MPP, PHTTP, G15 and/or 2-BR after induction of oxidative stress. (A) LDH activity was significantly decreased by treatment with E4 alone or in combination with ER-α inhibitor MPP compared to the H 2 O 2 -treated cell cultures or cultures combinedly treated by E4 + MPP + PHTTP. Combined use of MPP and PHTTP significantly increased the LDH activity compared to the cells treated by E4 alone or in combination with MPP. (B) LDH activity was significantly decreased by treatment with E4 alone or in combination with ER-β inhibitor PHTTP compared to the H 2 O 2 -treated cell cultures or cultures combinedly treated by E4 + MPP + PHTTP. Combined use of MPP and PHTTP significantly increased the LDH compared to the cell cultures treated by E4 alone or in combination with PHTTP. (C) Inhibition of palmitoylation alone or in combination with MPP significantly downregulated LDH activity compared to the H 2 O 2 -treated cells or to those treated by E4 alone. Combination of E4 with 2-BR, MPP and PHTTP significantly upregulated LDH activity compared to the cell cultures treated by E4 or 2-BR alone or in combination with MPP. (D) Cell cultures treated by E4 alone or in combination with GPR30 inhibitor G15 had significantly lower LDH activity compared to the cultures treated by H 2 O 2 alone. No significant difference was observed between the cells treated by E4 alone or in combination with G15.

    Article Snippet: Similar pattern of activities was observed when E4 was used in combination with PHTTP alone, and only the combined use of E4 with MPP and PHTTP completely blocked the E4-dependent effect by increasing the LDH activity to the same levels than that in the H2 O2 -treated cells ( ).

    Techniques: Activity Assay, Inhibition

    Apigenin decreases the oxidative stress in MI/R-induced H9C2 cells. H9C2 cells were treated with MI/R, and 1, 6 and 25 µM apigenin + MI/R. The levels of (A) LDH, (B) MDA, (C) CAT (D) and ATPase in H9C2 cells was determined using commercial kits. *P

    Journal: Molecular Medicine Reports

    Article Title: Apigenin suppresses the apoptosis of H9C2 rat cardiomyocytes subjected to myocardial ischemia-reperfusion injury via upregulation of the PI3K/Akt pathway

    doi: 10.3892/mmr.2018.9115

    Figure Lengend Snippet: Apigenin decreases the oxidative stress in MI/R-induced H9C2 cells. H9C2 cells were treated with MI/R, and 1, 6 and 25 µM apigenin + MI/R. The levels of (A) LDH, (B) MDA, (C) CAT (D) and ATPase in H9C2 cells was determined using commercial kits. *P

    Article Snippet: Cells were subsequently collected and the content/activity of lactate dehydrogenase (LDH), malondialdehyde (MDA), catalase (CAT), Na+ K+ -ATPase and Ca2+ -ATPase were determined using a LDH Assay Kit (cat. no. ab102526), MDA Assay Kit (cat. no. ab118970) (both from Abcam, Cambridge, UK), CAT Assay Kit (cat. no. BC0205), Na+ K+ -ATPase assay kit (cat. no. BC0065) and Ca2+ -ATPase assay kit (cat. no. BC0960) (all from Beijing Solarbio Science & Technology Co., Ltd.), respectively, in accordance with the manufacturers' protocol.

    Techniques: Multiple Displacement Amplification

    Lipid and protein analysis of sucrose gradient fractions prepared from Caco-2/TC7 cells Caco-2/TC7 cells were cultured on semi-permeable filters for 3 weeks for differentiation and then supplied with lipid micelles for 24 h. For lipid analysis, lipid micelles were supplemented with [1- 14 C]OA. Cell homogenates were centrifuged for 10 min at 1000 g and the supernatant was fractionated on a sucrose gradient. The top to bottom fractions (1–13) were analysed for lipids and proteins. ( A ) Autoradiography of a representative TLC of radiolabelled lipids extracted from cell homogenates, supernatants and each fraction of the sucrose gradient (FA, fatty acids; DG, diacylglycerol). ( B ) The radioactive bands from TLC were excised and the radioactivity was quantified by scintillation counting to evaluate the incorporation of [1- 14 C]OA into lipids. Results were expressed for each fraction as percentage of total [1- 14 C]OA incorporated into each lipid class. ( C ) Distribution of PL (◆) and TAG (□) in the sucrose gradient fractions. Results are expressed as percentage of total [1- 14 C]OA incorporated. ( D ) Immunoblotting of PLIN-2 (lipid droplet marker), LDH (cytosolic marker), PDI and calnexin (ER markers), GM130 (Golgi matrix protein marker), HSP60 and prohibitin (mitochondrial markers) and catalase (peroxisome marker). Immunoblotting of ApoB48 was performed to detect any contamination of the lipid droplet fraction with TRLs. Equal volumes of homogenate and supernatant were loaded on gels to evaluate the recovery/loss of material after the 1000 g centrifugation step. The same percentage of each fraction of the sucrose gradient was loaded on gels, except for fractions 1 and 2, which were 2-fold loaded in order to evaluate the presence of organelle markers with a greater sensitivity.

    Journal: Biology of the Cell

    Article Title: The proteome of cytosolic lipid droplets isolated from differentiated Caco-2/TC7 enterocytes reveals cell-specific characteristics

    doi: 10.1042/BC20110024

    Figure Lengend Snippet: Lipid and protein analysis of sucrose gradient fractions prepared from Caco-2/TC7 cells Caco-2/TC7 cells were cultured on semi-permeable filters for 3 weeks for differentiation and then supplied with lipid micelles for 24 h. For lipid analysis, lipid micelles were supplemented with [1- 14 C]OA. Cell homogenates were centrifuged for 10 min at 1000 g and the supernatant was fractionated on a sucrose gradient. The top to bottom fractions (1–13) were analysed for lipids and proteins. ( A ) Autoradiography of a representative TLC of radiolabelled lipids extracted from cell homogenates, supernatants and each fraction of the sucrose gradient (FA, fatty acids; DG, diacylglycerol). ( B ) The radioactive bands from TLC were excised and the radioactivity was quantified by scintillation counting to evaluate the incorporation of [1- 14 C]OA into lipids. Results were expressed for each fraction as percentage of total [1- 14 C]OA incorporated into each lipid class. ( C ) Distribution of PL (◆) and TAG (□) in the sucrose gradient fractions. Results are expressed as percentage of total [1- 14 C]OA incorporated. ( D ) Immunoblotting of PLIN-2 (lipid droplet marker), LDH (cytosolic marker), PDI and calnexin (ER markers), GM130 (Golgi matrix protein marker), HSP60 and prohibitin (mitochondrial markers) and catalase (peroxisome marker). Immunoblotting of ApoB48 was performed to detect any contamination of the lipid droplet fraction with TRLs. Equal volumes of homogenate and supernatant were loaded on gels to evaluate the recovery/loss of material after the 1000 g centrifugation step. The same percentage of each fraction of the sucrose gradient was loaded on gels, except for fractions 1 and 2, which were 2-fold loaded in order to evaluate the presence of organelle markers with a greater sensitivity.

    Article Snippet: Rabbit polyclonal antibodies to LDH, prohibitin and HSP60 were obtained from Abcam.

    Techniques: Cell Culture, Autoradiography, Thin Layer Chromatography, Radioactivity, Marker, Centrifugation

    Correlations between serum LDL/HDL ratio and PaO 2 (A) or LDH (B) . Serum LDL-C/HDL-C ratios correlated negatively with PaO 2 levels (r = -0.403, p = 0.027) and positively with LDH (r = 0.381, p = 0.034), n = 33.

    Journal: Lipids in Health and Disease

    Article Title: Impaired lipid metabolism in idiopathic pulmonary alveolar proteinosis

    doi: 10.1186/1476-511X-10-54

    Figure Lengend Snippet: Correlations between serum LDL/HDL ratio and PaO 2 (A) or LDH (B) . Serum LDL-C/HDL-C ratios correlated negatively with PaO 2 levels (r = -0.403, p = 0.027) and positively with LDH (r = 0.381, p = 0.034), n = 33.

    Article Snippet: The levels of serum TC, TG, HDL-C and LDL-C, fasting serum glucose, and lactate dehydrogenase (LDH) were measured with commercial kits using an automated chemistry analyzer (Olympus AU5400, Japan).

    Techniques:

    Effect of anti-OPN Ab treatment on plasma levels of organ injury markers and proinflammatory cytokines and chemokine in CLP animals . Sepsis was induced in mice by CLP and anti-OPN Ab or non-immunized IgG control at a dose of 50 μg/mice in 100 μl volumes was injected through the jugular vein. In the vehicle group, 100 μl of PBS was injected in CLP mice via the jugular vein. Blood samples were drawn by cardiac puncture at 20 h of sham-operated, vehicle and anti-OPN Ab-treated mice for measuring (A) ALT, (B) AST and (C) LDH. Similarly, the blood samples collected at 20 h after CLP were measured for (D) IL-6, (E) IL-1β and (F) MIP-2 by ELISA. Data are expressed as means ± SEM (n = 5 mice/group) and compared by one-way ANOVA and SNK method ( * P

    Journal: Critical Care

    Article Title: Neutralization of osteopontin attenuates neutrophil migration in sepsis-induced acute lung injury

    doi: 10.1186/s13054-015-0782-3

    Figure Lengend Snippet: Effect of anti-OPN Ab treatment on plasma levels of organ injury markers and proinflammatory cytokines and chemokine in CLP animals . Sepsis was induced in mice by CLP and anti-OPN Ab or non-immunized IgG control at a dose of 50 μg/mice in 100 μl volumes was injected through the jugular vein. In the vehicle group, 100 μl of PBS was injected in CLP mice via the jugular vein. Blood samples were drawn by cardiac puncture at 20 h of sham-operated, vehicle and anti-OPN Ab-treated mice for measuring (A) ALT, (B) AST and (C) LDH. Similarly, the blood samples collected at 20 h after CLP were measured for (D) IL-6, (E) IL-1β and (F) MIP-2 by ELISA. Data are expressed as means ± SEM (n = 5 mice/group) and compared by one-way ANOVA and SNK method ( * P

    Article Snippet: Measurement of organ injury markers Plasma levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) were measured using commercial assay kits (Pointe Scientific, Lincoln Park, MI, USA) according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Injection, AST Assay, Enzyme-linked Immunosorbent Assay

    Cytotoxic effect of carbapenem-susceptible and carbapenem-resistant A . baumannii strains on HaCaT keratinocyte cells. Cytotoxic levels caused by carbapenem-susceptible strain 813 and carbapenem-resistant strains 1995/12 and 4031 on HaCaT cells were measured by LDH assay. Values are the means from results obtained in triplicate. Error bars represent the standard deviation of the mean value.

    Journal: PLoS ONE

    Article Title: Acinetobacter spp. porin Omp33-36: Classification and transcriptional response to carbapenems and host cells

    doi: 10.1371/journal.pone.0201608

    Figure Lengend Snippet: Cytotoxic effect of carbapenem-susceptible and carbapenem-resistant A . baumannii strains on HaCaT keratinocyte cells. Cytotoxic levels caused by carbapenem-susceptible strain 813 and carbapenem-resistant strains 1995/12 and 4031 on HaCaT cells were measured by LDH assay. Values are the means from results obtained in triplicate. Error bars represent the standard deviation of the mean value.

    Article Snippet: Cytotoxicity Assay The cytotoxicity levels after infection of HaCaT keratinocytes with A . baumannii cells were measured with a lactate dehydrogenase (LDH) Cytotoxicity Assay Kit (Thermo Scientific, Lithuania).

    Techniques: Lactate Dehydrogenase Assay, Standard Deviation

    LDH activities of cultured human blood cells treated with 10, 25, 50, 75, 100, 150 and 200 mg/L of TPO for 24 and 48 h (Control: whole blood without terpinolene. Each value represents mean ± SD of five experiments;

    Journal: Cytotechnology

    Article Title: Genotoxic and oxidative damage potentials in human lymphocytes after exposure to terpinolene in vitro

    doi: 10.1007/s10616-014-9698-z

    Figure Lengend Snippet: LDH activities of cultured human blood cells treated with 10, 25, 50, 75, 100, 150 and 200 mg/L of TPO for 24 and 48 h (Control: whole blood without terpinolene. Each value represents mean ± SD of five experiments;

    Article Snippet: Human blood cells were placed in a 24-well culture plate and treated with 10, 25, 50, 75, 100, 150 and 200 mg/L of TPO for 24 and 48 h. Lactate dehydrogenase (LDH) released from damaged cells in culture medium was quantified by using LDH assay kit (Cayman Chemical Company® , Ann Arbor, MI, USA).

    Techniques: Cell Culture