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    AceGLAmp Taq 2X PCR Master Mix combines high quality recombinant Taq klenow DNA Polymerase with a trace amount of Deep Ocean DNA Polymerase The 3 →5 exonuclease activity of Deep
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    99
    TaKaRa la taq
    Impaired µ–γ1 switch recombination of DC3-5Rα –/– B cells in response to IL-5 plus CS/2. Splenic B cells (2·5 × 10 6 in a 5-ml culture) were cultured with CS/2 (0·5 µg/ml) or CS/2 (0·5 µg/ml) plus IL-5 (100 U/ml). On day 2, cells were harvested, the cell number was adjusted to 2·5 × 10 6 /5 ml and the cells were re-cultured with relevant stimuli for 24 hr. On day 3, total DNA were prepared from live cells and the DNA samples (50 and 100 ng) were amplified using <t>5′Sγ1</t> and 3′Sµ primers and LA- <t>Taq</t> polymerase and hybridized with 5′Sγ1 probes.
    La Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Toyobo la taq
    Impaired µ–γ1 switch recombination of DC3-5Rα –/– B cells in response to IL-5 plus CS/2. Splenic B cells (2·5 × 10 6 in a 5-ml culture) were cultured with CS/2 (0·5 µg/ml) or CS/2 (0·5 µg/ml) plus IL-5 (100 U/ml). On day 2, cells were harvested, the cell number was adjusted to 2·5 × 10 6 /5 ml and the cells were re-cultured with relevant stimuli for 24 hr. On day 3, total DNA were prepared from live cells and the DNA samples (50 and 100 ng) were amplified using <t>5′Sγ1</t> and 3′Sµ primers and LA- <t>Taq</t> polymerase and hybridized with 5′Sγ1 probes.
    La Taq, supplied by Toyobo, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Fisher Scientific la taq kit
    Impaired µ–γ1 switch recombination of DC3-5Rα –/– B cells in response to IL-5 plus CS/2. Splenic B cells (2·5 × 10 6 in a 5-ml culture) were cultured with CS/2 (0·5 µg/ml) or CS/2 (0·5 µg/ml) plus IL-5 (100 U/ml). On day 2, cells were harvested, the cell number was adjusted to 2·5 × 10 6 /5 ml and the cells were re-cultured with relevant stimuli for 24 hr. On day 3, total DNA were prepared from live cells and the DNA samples (50 and 100 ng) were amplified using <t>5′Sγ1</t> and 3′Sµ primers and LA- <t>Taq</t> polymerase and hybridized with 5′Sγ1 probes.
    La Taq Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 83/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq buffer
    Impaired µ–γ1 switch recombination of DC3-5Rα –/– B cells in response to IL-5 plus CS/2. Splenic B cells (2·5 × 10 6 in a 5-ml culture) were cultured with CS/2 (0·5 µg/ml) or CS/2 (0·5 µg/ml) plus IL-5 (100 U/ml). On day 2, cells were harvested, the cell number was adjusted to 2·5 × 10 6 /5 ml and the cells were re-cultured with relevant stimuli for 24 hr. On day 3, total DNA were prepared from live cells and the DNA samples (50 and 100 ng) were amplified using <t>5′Sγ1</t> and 3′Sµ primers and LA- <t>Taq</t> polymerase and hybridized with 5′Sγ1 probes.
    La Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq enzyme
    PCR amplification of the <t>FimA</t> gene, recombinant plasmid construction and sequence identification. (A) The LA <t>Taq</t> enzyme was used to amplify the FimA gene. Using the extracted DNA as a template, the target gene was amplified by PCR. Electrophoretic detection demonstrated a distinct band with a molecular weight of 1,000 bp, which was consistent with the size of FimA (1,044 bp). Lanes 1 and 2 are FimA target segments. (B) Xho I/ Bam H I double-digested PMD19-T-FimA plasmid. Lanes 1 and 2 are the PMD19-T-FimA plasmid. (C) Xho I/ Bam H I double-digested PGEX-6P-1 plasmid. Lanes 1 and 2 are the PGEX-6P-1 plasmid. (D) Xho I/ Bam H I double-digested PGEX-6P-FimA plasmid. Lanes 1 and 2 are PGEX-6P-FimA plasmid. PCR, polymerase chain reaction; FimA, Porphyromonas gingivalis fimbriae; M, marker.
    La Taq Enzyme, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq kit
    PCR amplification of the <t>FimA</t> gene, recombinant plasmid construction and sequence identification. (A) The LA <t>Taq</t> enzyme was used to amplify the FimA gene. Using the extracted DNA as a template, the target gene was amplified by PCR. Electrophoretic detection demonstrated a distinct band with a molecular weight of 1,000 bp, which was consistent with the size of FimA (1,044 bp). Lanes 1 and 2 are FimA target segments. (B) Xho I/ Bam H I double-digested PMD19-T-FimA plasmid. Lanes 1 and 2 are the PMD19-T-FimA plasmid. (C) Xho I/ Bam H I double-digested PGEX-6P-1 plasmid. Lanes 1 and 2 are the PGEX-6P-1 plasmid. (D) Xho I/ Bam H I double-digested PGEX-6P-FimA plasmid. Lanes 1 and 2 are PGEX-6P-FimA plasmid. PCR, polymerase chain reaction; FimA, Porphyromonas gingivalis fimbriae; M, marker.
    La Taq Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 325 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq system
    PCR amplification of the <t>FimA</t> gene, recombinant plasmid construction and sequence identification. (A) The LA <t>Taq</t> enzyme was used to amplify the FimA gene. Using the extracted DNA as a template, the target gene was amplified by PCR. Electrophoretic detection demonstrated a distinct band with a molecular weight of 1,000 bp, which was consistent with the size of FimA (1,044 bp). Lanes 1 and 2 are FimA target segments. (B) Xho I/ Bam H I double-digested PMD19-T-FimA plasmid. Lanes 1 and 2 are the PMD19-T-FimA plasmid. (C) Xho I/ Bam H I double-digested PGEX-6P-1 plasmid. Lanes 1 and 2 are the PGEX-6P-1 plasmid. (D) Xho I/ Bam H I double-digested PGEX-6P-FimA plasmid. Lanes 1 and 2 are PGEX-6P-FimA plasmid. PCR, polymerase chain reaction; FimA, Porphyromonas gingivalis fimbriae; M, marker.
    La Taq System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa premix la taq
    PCR amplification of the <t>FimA</t> gene, recombinant plasmid construction and sequence identification. (A) The LA <t>Taq</t> enzyme was used to amplify the FimA gene. Using the extracted DNA as a template, the target gene was amplified by PCR. Electrophoretic detection demonstrated a distinct band with a molecular weight of 1,000 bp, which was consistent with the size of FimA (1,044 bp). Lanes 1 and 2 are FimA target segments. (B) Xho I/ Bam H I double-digested PMD19-T-FimA plasmid. Lanes 1 and 2 are the PMD19-T-FimA plasmid. (C) Xho I/ Bam H I double-digested PGEX-6P-1 plasmid. Lanes 1 and 2 are the PGEX-6P-1 plasmid. (D) Xho I/ Bam H I double-digested PGEX-6P-FimA plasmid. Lanes 1 and 2 are PGEX-6P-FimA plasmid. PCR, polymerase chain reaction; FimA, Porphyromonas gingivalis fimbriae; M, marker.
    Premix La Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher la taq enzyme
    PCR amplification of the <t>FimA</t> gene, recombinant plasmid construction and sequence identification. (A) The LA <t>Taq</t> enzyme was used to amplify the FimA gene. Using the extracted DNA as a template, the target gene was amplified by PCR. Electrophoretic detection demonstrated a distinct band with a molecular weight of 1,000 bp, which was consistent with the size of FimA (1,044 bp). Lanes 1 and 2 are FimA target segments. (B) Xho I/ Bam H I double-digested PMD19-T-FimA plasmid. Lanes 1 and 2 are the PMD19-T-FimA plasmid. (C) Xho I/ Bam H I double-digested PGEX-6P-1 plasmid. Lanes 1 and 2 are the PGEX-6P-1 plasmid. (D) Xho I/ Bam H I double-digested PGEX-6P-FimA plasmid. Lanes 1 and 2 are PGEX-6P-FimA plasmid. PCR, polymerase chain reaction; FimA, Porphyromonas gingivalis fimbriae; M, marker.
    La Taq Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher takara la taq
    PCR amplification of the <t>FimA</t> gene, recombinant plasmid construction and sequence identification. (A) The LA <t>Taq</t> enzyme was used to amplify the FimA gene. Using the extracted DNA as a template, the target gene was amplified by PCR. Electrophoretic detection demonstrated a distinct band with a molecular weight of 1,000 bp, which was consistent with the size of FimA (1,044 bp). Lanes 1 and 2 are FimA target segments. (B) Xho I/ Bam H I double-digested PMD19-T-FimA plasmid. Lanes 1 and 2 are the PMD19-T-FimA plasmid. (C) Xho I/ Bam H I double-digested PGEX-6P-1 plasmid. Lanes 1 and 2 are the PGEX-6P-1 plasmid. (D) Xho I/ Bam H I double-digested PGEX-6P-FimA plasmid. Lanes 1 and 2 are PGEX-6P-FimA plasmid. PCR, polymerase chain reaction; FimA, Porphyromonas gingivalis fimbriae; M, marker.
    Takara La Taq, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq hotstart
    Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA <t>Taq</t> (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    La Taq Hotstart, supplied by TaKaRa, used in various techniques. Bioz Stars score: 76/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq hs
    Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA <t>Taq</t> (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    La Taq Hs, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa fidelity la taq
    Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA <t>Taq</t> (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    Fidelity La Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 84/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq tm
    Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA <t>Taq</t> (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    La Taq Tm, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    TaKaRa takala la taq
    Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA <t>Taq</t> (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    Takala La Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 81/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cambrex takara la taq
    Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA <t>Taq</t> (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    Takara La Taq, supplied by Cambrex, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    TaKaRa la taq polymerase
    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the <t>Taq</t> DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; <t>Takara-bio).</t> PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.
    La Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 4252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher la taq polymerase
    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the <t>Taq</t> DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; <t>Takara-bio).</t> PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.
    La Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq premix buffer
    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the <t>Taq</t> DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; <t>Takara-bio).</t> PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.
    La Taq Premix Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore la taq polymerase
    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the <t>Taq</t> DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; <t>Takara-bio).</t> PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.
    La Taq Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq hot startversion
    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the <t>Taq</t> DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; <t>Takara-bio).</t> PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.
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    TaKaRa 25ul la taq enzyme
    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the <t>Taq</t> DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; <t>Takara-bio).</t> PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.
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    TaKaRa la taq amplification kit
    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the <t>Taq</t> DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; <t>Takara-bio).</t> PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.
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    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the <t>Taq</t> DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; <t>Takara-bio).</t> PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.
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    Becton Dickinson la taq dna polymerase
    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the <t>Taq</t> DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; <t>Takara-bio).</t> PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.
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    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the <t>Taq</t> DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; <t>Takara-bio).</t> PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.
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    TaKaRa la taq high fidelity polymerase
    Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA <t>Taq</t> polymerase HS. M is the <t>DNA</t> ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region
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    Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA <t>Taq</t> polymerase HS. M is the <t>DNA</t> ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region
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    Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA <t>Taq</t> polymerase HS. M is the <t>DNA</t> ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region
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    Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA <t>Taq</t> polymerase HS. M is the <t>DNA</t> ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region
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    Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA <t>Taq</t> polymerase HS. M is the <t>DNA</t> ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region
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    Image Search Results


    Impaired µ–γ1 switch recombination of DC3-5Rα –/– B cells in response to IL-5 plus CS/2. Splenic B cells (2·5 × 10 6 in a 5-ml culture) were cultured with CS/2 (0·5 µg/ml) or CS/2 (0·5 µg/ml) plus IL-5 (100 U/ml). On day 2, cells were harvested, the cell number was adjusted to 2·5 × 10 6 /5 ml and the cells were re-cultured with relevant stimuli for 24 hr. On day 3, total DNA were prepared from live cells and the DNA samples (50 and 100 ng) were amplified using 5′Sγ1 and 3′Sµ primers and LA- Taq polymerase and hybridized with 5′Sγ1 probes.

    Journal: Immunology

    Article Title: Functional dissection of the cytoplasmic subregions of the interleukin-5 receptor ? chain in growth and immunoglobulin G1 switch recombination of B cells

    doi: 10.1046/j.1365-2567.2001.01196.x

    Figure Lengend Snippet: Impaired µ–γ1 switch recombination of DC3-5Rα –/– B cells in response to IL-5 plus CS/2. Splenic B cells (2·5 × 10 6 in a 5-ml culture) were cultured with CS/2 (0·5 µg/ml) or CS/2 (0·5 µg/ml) plus IL-5 (100 U/ml). On day 2, cells were harvested, the cell number was adjusted to 2·5 × 10 6 /5 ml and the cells were re-cultured with relevant stimuli for 24 hr. On day 3, total DNA were prepared from live cells and the DNA samples (50 and 100 ng) were amplified using 5′Sγ1 and 3′Sµ primers and LA- Taq polymerase and hybridized with 5′Sγ1 probes.

    Article Snippet: For amplification of γ1–µ recombination products, 200 ng of total DNA from cultured or freshly isolated B cells were subjected to PCR amplification in 50 µl volumes containing LA PCR Buffer II (Takara; Kyoto, Japan), 2·5 m m MgCl2 , 0·4 m m dNTP, 2·5 U LA Taq (Takara), 1 µ m sense-strand 5′Sγ1 primer (5′-CACTCCTGGGTATGGAAACACATCCTAC-3′; nucleotides 262–289 in region 5′ of the Sγ1 repeats; MUSIGHANB) in combination with an anti-sense 3′Sµ primer (5′-AGCCTAACTTATCTGAGCCTAGTTCAAC-3′; nucleotides 1347–1319 in region 3′ of the Sµ repeats; MUSIGCD09).

    Techniques: Cell Culture, Amplification

    The error-suppressing effects of ttRecA and ttMutS in the presence of ATP. (a) A schematic representation for the mechanism by which ttMutS suppresses nonspecific amplifications during PCR. A ttMutS dimer recognizes mismatched bases generated by mishybridization of the primer and blocks the approach of DNA polymerase. (b) A schematic representation for the mechanism by which ttRecA suppresses nonspecific amplification during PCR. ttRecA promotes proper priming for PCR. (c) A 423 bp region of the ttha1806 gene was amplified by using Takara LA Taq in the presence of 0 to 0.4 mM ATP. Lanes 1–4, 5–8, and 9–12 are the results of the reaction without ttMutS or ttRecA, with 0.8 μ M ttMutS, and with 0.4 μ M ttRecA, respectively. The amounts of the amplified fragments were quantified by using the ImageJ software [ 9 ] and are shown as bar graphs in the lower panels, where gray and blue indicate nonspecific and desired amplifications, respectively. (d) A 423 bp region of the ttha1806 gene was amplified by using Takara LA Taq in the presence of 0.9, 2.7, 8.0, or 24 ng/mL template DNA ( T. thermophilus HB8 genomic DNA). The relative amounts of the amplified fragments are shown. Gray and blue bars indicate nonspecific and desired amplifications, respectively.

    Journal: Journal of Nucleic Acids

    Article Title: Simultaneous Use of MutS and RecA for Suppression of Nonspecific Amplification during PCR

    doi: 10.1155/2013/823730

    Figure Lengend Snippet: The error-suppressing effects of ttRecA and ttMutS in the presence of ATP. (a) A schematic representation for the mechanism by which ttMutS suppresses nonspecific amplifications during PCR. A ttMutS dimer recognizes mismatched bases generated by mishybridization of the primer and blocks the approach of DNA polymerase. (b) A schematic representation for the mechanism by which ttRecA suppresses nonspecific amplification during PCR. ttRecA promotes proper priming for PCR. (c) A 423 bp region of the ttha1806 gene was amplified by using Takara LA Taq in the presence of 0 to 0.4 mM ATP. Lanes 1–4, 5–8, and 9–12 are the results of the reaction without ttMutS or ttRecA, with 0.8 μ M ttMutS, and with 0.4 μ M ttRecA, respectively. The amounts of the amplified fragments were quantified by using the ImageJ software [ 9 ] and are shown as bar graphs in the lower panels, where gray and blue indicate nonspecific and desired amplifications, respectively. (d) A 423 bp region of the ttha1806 gene was amplified by using Takara LA Taq in the presence of 0.9, 2.7, 8.0, or 24 ng/mL template DNA ( T. thermophilus HB8 genomic DNA). The relative amounts of the amplified fragments are shown. Gray and blue bars indicate nonspecific and desired amplifications, respectively.

    Article Snippet: The 423-base pair (bp) region of the ttha1806 gene was amplified by Takara LA Taq using T. thermophilus HB8 genomic DNA as the template.

    Techniques: Polymerase Chain Reaction, Generated, Amplification, Software

    PCR amplification of the FimA gene, recombinant plasmid construction and sequence identification. (A) The LA Taq enzyme was used to amplify the FimA gene. Using the extracted DNA as a template, the target gene was amplified by PCR. Electrophoretic detection demonstrated a distinct band with a molecular weight of 1,000 bp, which was consistent with the size of FimA (1,044 bp). Lanes 1 and 2 are FimA target segments. (B) Xho I/ Bam H I double-digested PMD19-T-FimA plasmid. Lanes 1 and 2 are the PMD19-T-FimA plasmid. (C) Xho I/ Bam H I double-digested PGEX-6P-1 plasmid. Lanes 1 and 2 are the PGEX-6P-1 plasmid. (D) Xho I/ Bam H I double-digested PGEX-6P-FimA plasmid. Lanes 1 and 2 are PGEX-6P-FimA plasmid. PCR, polymerase chain reaction; FimA, Porphyromonas gingivalis fimbriae; M, marker.

    Journal: International Journal of Molecular Medicine

    Article Title: Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF-κB signaling pathway in human peripheral blood mononuclear cells

    doi: 10.3892/ijmm.2019.4069

    Figure Lengend Snippet: PCR amplification of the FimA gene, recombinant plasmid construction and sequence identification. (A) The LA Taq enzyme was used to amplify the FimA gene. Using the extracted DNA as a template, the target gene was amplified by PCR. Electrophoretic detection demonstrated a distinct band with a molecular weight of 1,000 bp, which was consistent with the size of FimA (1,044 bp). Lanes 1 and 2 are FimA target segments. (B) Xho I/ Bam H I double-digested PMD19-T-FimA plasmid. Lanes 1 and 2 are the PMD19-T-FimA plasmid. (C) Xho I/ Bam H I double-digested PGEX-6P-1 plasmid. Lanes 1 and 2 are the PGEX-6P-1 plasmid. (D) Xho I/ Bam H I double-digested PGEX-6P-FimA plasmid. Lanes 1 and 2 are PGEX-6P-FimA plasmid. PCR, polymerase chain reaction; FimA, Porphyromonas gingivalis fimbriae; M, marker.

    Article Snippet: The FimA gene was amplified by LA Taq enzyme (Takara Biotechnology Co., Ltd., Dalian, China).

    Techniques: Polymerase Chain Reaction, Amplification, Recombinant, Plasmid Preparation, Sequencing, Molecular Weight, Marker

    Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.

    Journal: International Journal of Molecular Sciences

    Article Title: Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR)

    doi: 10.3390/ijms14036436

    Figure Lengend Snippet: Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.

    Article Snippet: PCR was performed with 0.06 units/μL LA Taq Hot-Start Version (Takara, Shiga, Japan), 0.03 units/μL KOD polymerase (Toyobo, Tokyo, Japan) or 40 nM A. aeolicus DnaE, in 1× Takara LA PCR buffer II (Takara, Shiga, Japan) containing 20 nM template DNA; 400 nM primers; 400 μM dATP, dTTP, dCTP and dGTP and various concentrations of Tth MutS or Aae MutS.

    Techniques: Polymerase Chain Reaction, Amplification, Concentration Assay

    Effect of the number of mismatches in an 80-bp template on amplification efficiency in the presence of Tth MutS. ( A ) Templates containing zero, one, two or three unpaired T mismatches were used. PCR was performed using LA Taq Hot-Start version with perfectly matched primers in the absence or presence of 0.35 μM Tth MutS; ( B ) Quantification of the relative amounts of amplified fragments. Gray and blue columns indicate amplification in the absence or presence of Tth MutS, respectively. The amounts of the products were normalized by that from perfectly-matched template at 0 μM Tth MutS; ( C ) Model for the mechanism by which Tth MutS suppresses amplification from mismatched templates.

    Journal: International Journal of Molecular Sciences

    Article Title: Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR)

    doi: 10.3390/ijms14036436

    Figure Lengend Snippet: Effect of the number of mismatches in an 80-bp template on amplification efficiency in the presence of Tth MutS. ( A ) Templates containing zero, one, two or three unpaired T mismatches were used. PCR was performed using LA Taq Hot-Start version with perfectly matched primers in the absence or presence of 0.35 μM Tth MutS; ( B ) Quantification of the relative amounts of amplified fragments. Gray and blue columns indicate amplification in the absence or presence of Tth MutS, respectively. The amounts of the products were normalized by that from perfectly-matched template at 0 μM Tth MutS; ( C ) Model for the mechanism by which Tth MutS suppresses amplification from mismatched templates.

    Article Snippet: PCR was performed with 0.06 units/μL LA Taq Hot-Start Version (Takara, Shiga, Japan), 0.03 units/μL KOD polymerase (Toyobo, Tokyo, Japan) or 40 nM A. aeolicus DnaE, in 1× Takara LA PCR buffer II (Takara, Shiga, Japan) containing 20 nM template DNA; 400 nM primers; 400 μM dATP, dTTP, dCTP and dGTP and various concentrations of Tth MutS or Aae MutS.

    Techniques: Amplification, Polymerase Chain Reaction

    PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the Taq DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; Takara-bio). PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.

    Journal: Nucleic Acids Research

    Article Title: Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products

    doi: 10.1093/nar/gni111

    Figure Lengend Snippet: PCR of subdivided genomic sequences. Three regions of human genomic DNA (GenBank accession nos AC006454 , AC093734 and X91835 , with 5103, 5193 and 12 114 bp, respectively) were subdivided into nine 567, 577 and 1346 bp PCR sites, respectively. PCR was performed for each subdivided site using primer sets (20 bp each) corresponding to the terminal sequence of each site using the Taq DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; Takara-bio). PCR was carried out in the absence and in the presence of Tth RecA protein and ATP. The products were electrophoresed and stained with ethidium bromide. ( a ) A diagrammatic representation of the subdivided region (5103 bp in GenBank accession no AC006454 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( b ) A diagrammatic representation of the subdivided region (5193 bp in GenBank accession no AC093734 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). ( c ) A diagrammatic representation of the subdivided region (12 114 bp in GenBank accession no X91835 ) (upper panel) and the electrophoretic patterns of the PCR products (lower panel). Throughout (a–c), nine subdivided sites for each region are indicated as a-1 to a-9, b-1 to b-9 and c-1 to c-9. Nucleotide (nt) numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.

    Article Snippet: The same results were obtained by the other polymerase systems: LA Taq polymerase (Takara-bio), Tth polymerase (Applied-boisystems); Expand High Fidelity, Expand High FidelityPLUS and Expand Long Template polymerase (Roche-diagnostics); TITANIUM Taq polymerase (Becton-Dickinson-Clontech); and Taq polymerase (Promega).

    Techniques: Polymerase Chain Reaction, Genomic Sequencing, Sequencing, Staining

    PCR with primers carrying mismatched bases. PCR was performed at two human genomic sites with primers (20 bp), one of which (forward primer) carried one, two or three mismatched bases in the middle of the primer, in the absence (left) or presence (right) of Tth RecA protein and ATP using the Taq DNA polymerase ( rTaq DNA polymerase plus ‘hot start’ antibody). ( a ) Upper panel: a diagrammatic representation of the location of the PCR site (20 bp between nt 66 562 and nt 66 581 in GenBank accession no AC006454 ) and of the primers. Lower panel: lanes 1 and 5, PCR products using primers without mismatched bases (primer set a-1); lanes 2 and 6, PCR products using primers (primer set a-2 with one mismatched base at nt 66 566, T to C); lanes 3 and 7, PCR products using primers (primer set a-3 with two mismatched base at nt 66 566 and nt 66 571, both T to C); and lanes 4 and 8, PCR products using primers (primer set a-4 with three mismatched base at nt 66 566 and nt 66 571, T to C and nt 66 576, G to C). The oligonucleotide sequences used for the forward primers (mismatched bases are underlined) are as follows: primer set a-1, 5′-CATGGCACCTGCTCTGAGAC-3′; primer set a-2, 5′-CATGGCACC C GCTCTGAGAC-3′; primer set a-3, 5′-CATGGCACC C GCTC C GAGAC-3′; and primer set a-4, 5′-CATG C CACC C GCTC C GAGAC-3′. ( b ) Upper panel: a diagrammatic representation of the location of the PCR site (20 bp between nt 38 501 and nt 38 520 in GenBank accession no. AC0937734 ) and of the primers. Lower panel: lanes 1 and 5, PCR products using primers without mismatched bases (primer set b-1); lanes 2 and 6, PCR products using primers (primer set b-2 with one mismatched base at nt 38 505, G to A); lanes 3 and 7, PCR products using primers (primer set b-3 with two mismatched base at nt 38 505 and nt 38 510, both G to A); and lanes 4 and 8, PCR products using primers (primer set b-4 with three mismatched base at nt 38 505, nt 38 510 and nt 38 515, all G to A). The oligonucleotide sequences used for the forward primers are as follows: primer set b-1, 5′-ATCTGTGTGGTTCGGCTCTG-3′; primer set b-2, 5′-ATCTGTGTG A TTCGGCTCTG-3′; primer set b-3, 5′-ATCTGTGTG A TTCG A CTCTG-3′; and primer set b-4, 5′-ATCT A TGTG A TTCG A CTCTG-3′. ( c ) Upper panel: a diagrammatic representation of the location of the PCR site (20 bp between nt 63 957 and nt 63 976 in GenBank accession no. AC004975 ) and of the primers. Lower panel: lanes 1 and 5, PCR products using primers without mismatched bases (primer set c-1); lanes 2 and 6, PCR products using primers (primer set c-2 with one mismatched base at nt 63 961, A to T); lanes 3 and 7, PCR products using primers (primer set c-3 with two mismatched base at nt 63 961 and nt 63 966, A to T and C to T); and lanes 4 and 8, PCR products using primers (primer set c-4 with three mismatched base at nt 63 961, nt 63 966 and nt 63 971, A to T, C to T and G to T). The oligonucleotide sequences used for the forward primers are as follows: primer set c-1, 5′-GCAGGCACCAAGAACTACTG-3′; primer set c-2, 5′-GCAGGCACC T AGAACTACTG-3′; primer set c-3, 5′-GCAGGCACC T AGAA T TACTG-3′; and primer set c-4, 5′-GCAG T CACC T AGAA T TACTG-3′. The sequences for the backward primers are 5′-TCACCTCCCAGCCTGGCCCA-3′ for ( a ), 5′-AGGGAGATGTTCTCATAAAT-3′ and 5′-CTGTAAGTGGCAGACATTAC-3′ for ( b ). Nucleotide numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.

    Journal: Nucleic Acids Research

    Article Title: Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products

    doi: 10.1093/nar/gni111

    Figure Lengend Snippet: PCR with primers carrying mismatched bases. PCR was performed at two human genomic sites with primers (20 bp), one of which (forward primer) carried one, two or three mismatched bases in the middle of the primer, in the absence (left) or presence (right) of Tth RecA protein and ATP using the Taq DNA polymerase ( rTaq DNA polymerase plus ‘hot start’ antibody). ( a ) Upper panel: a diagrammatic representation of the location of the PCR site (20 bp between nt 66 562 and nt 66 581 in GenBank accession no AC006454 ) and of the primers. Lower panel: lanes 1 and 5, PCR products using primers without mismatched bases (primer set a-1); lanes 2 and 6, PCR products using primers (primer set a-2 with one mismatched base at nt 66 566, T to C); lanes 3 and 7, PCR products using primers (primer set a-3 with two mismatched base at nt 66 566 and nt 66 571, both T to C); and lanes 4 and 8, PCR products using primers (primer set a-4 with three mismatched base at nt 66 566 and nt 66 571, T to C and nt 66 576, G to C). The oligonucleotide sequences used for the forward primers (mismatched bases are underlined) are as follows: primer set a-1, 5′-CATGGCACCTGCTCTGAGAC-3′; primer set a-2, 5′-CATGGCACC C GCTCTGAGAC-3′; primer set a-3, 5′-CATGGCACC C GCTC C GAGAC-3′; and primer set a-4, 5′-CATG C CACC C GCTC C GAGAC-3′. ( b ) Upper panel: a diagrammatic representation of the location of the PCR site (20 bp between nt 38 501 and nt 38 520 in GenBank accession no. AC0937734 ) and of the primers. Lower panel: lanes 1 and 5, PCR products using primers without mismatched bases (primer set b-1); lanes 2 and 6, PCR products using primers (primer set b-2 with one mismatched base at nt 38 505, G to A); lanes 3 and 7, PCR products using primers (primer set b-3 with two mismatched base at nt 38 505 and nt 38 510, both G to A); and lanes 4 and 8, PCR products using primers (primer set b-4 with three mismatched base at nt 38 505, nt 38 510 and nt 38 515, all G to A). The oligonucleotide sequences used for the forward primers are as follows: primer set b-1, 5′-ATCTGTGTGGTTCGGCTCTG-3′; primer set b-2, 5′-ATCTGTGTG A TTCGGCTCTG-3′; primer set b-3, 5′-ATCTGTGTG A TTCG A CTCTG-3′; and primer set b-4, 5′-ATCT A TGTG A TTCG A CTCTG-3′. ( c ) Upper panel: a diagrammatic representation of the location of the PCR site (20 bp between nt 63 957 and nt 63 976 in GenBank accession no. AC004975 ) and of the primers. Lower panel: lanes 1 and 5, PCR products using primers without mismatched bases (primer set c-1); lanes 2 and 6, PCR products using primers (primer set c-2 with one mismatched base at nt 63 961, A to T); lanes 3 and 7, PCR products using primers (primer set c-3 with two mismatched base at nt 63 961 and nt 63 966, A to T and C to T); and lanes 4 and 8, PCR products using primers (primer set c-4 with three mismatched base at nt 63 961, nt 63 966 and nt 63 971, A to T, C to T and G to T). The oligonucleotide sequences used for the forward primers are as follows: primer set c-1, 5′-GCAGGCACCAAGAACTACTG-3′; primer set c-2, 5′-GCAGGCACC T AGAACTACTG-3′; primer set c-3, 5′-GCAGGCACC T AGAA T TACTG-3′; and primer set c-4, 5′-GCAG T CACC T AGAA T TACTG-3′. The sequences for the backward primers are 5′-TCACCTCCCAGCCTGGCCCA-3′ for ( a ), 5′-AGGGAGATGTTCTCATAAAT-3′ and 5′-CTGTAAGTGGCAGACATTAC-3′ for ( b ). Nucleotide numbers correspond to registries in GenBank. Locations of the specific PCR products are indicated by arrows.

    Article Snippet: The same results were obtained by the other polymerase systems: LA Taq polymerase (Takara-bio), Tth polymerase (Applied-boisystems); Expand High Fidelity, Expand High FidelityPLUS and Expand Long Template polymerase (Roche-diagnostics); TITANIUM Taq polymerase (Becton-Dickinson-Clontech); and Taq polymerase (Promega).

    Techniques: Polymerase Chain Reaction

    Effect of T.thermophilus RecA protein on PCR. PCR with Taq DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; Takara-bio) for several randomly selected sequences (300–650 bp) in human genomic DNA was carried out in the absence or in the presence of the Tth RecA protein. ( a ) Control, PCR under the standard conditions described in Materials and Methods. ( b ) Similar to (a), but with Tth RecA protein (0.4 μg per 25 μl reaction mixture). ( c ) Similar to (a), but with ATP (400 μM). ( d ) Similar to (a), but with Tth RecA protein (0.4 μg per 25 μl reaction mixture) and ATP (300 μM). ( e ) Similar to (a), but with Tth RecA protein (0.4 μg per 25 μl reaction mixture) and ATP-γS (300 μM). The products were electrophoresed and stained with ethidium bromide. Molecular weight markers are indicated on the right-hand side of these panels. The oligonucleotide sequences used for the primers were as follows: 5′-ACAATGGGCTCACTCACCCA-3′ and 5′-CTAAGACCAATGGATAGCTG-3′ for lane 1 (300 bp); 5′-GCTCAGCATGGTGGTGGCAT-3′ and 5′-CCTCATACCTTCCCCCCCAT-3′ for lane 2 (319 bp); 5′-GACTACTCTAGCGACTGTCC-3′ and 5′-GACAGCCACCAGATCCAATC-3′ for lane 3 (360 bp); 5′-AACCTCACAACCTTGGCTGA-3′ and 5′-TTCACAACTTAAGATTTGGC-3′ for lane 4 (400 bp); 5′-AGGCAACTAGGATGGTGTGG-3′ and 5′-CAGGGAGCGTGTCCATAGGG-3′ for lane 5 (450 bp); 5′-CTGCTGAAAGAGATGCGGTG-3′ and 5′-AGGAAAACAGCCCAAGGGAC-3′ for lane 6 (469 bp); and 5′-ACTTTGTTCTGAGCCTCACA-3′ and 5′-GTTGCCCAATCGCCCCTCTC-3′ for lane 7 (650 bp).

    Journal: Nucleic Acids Research

    Article Title: Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products

    doi: 10.1093/nar/gni111

    Figure Lengend Snippet: Effect of T.thermophilus RecA protein on PCR. PCR with Taq DNA polymerase ( ExTaq DNA polymerase plus ‘hot start’ antibody; Takara-bio) for several randomly selected sequences (300–650 bp) in human genomic DNA was carried out in the absence or in the presence of the Tth RecA protein. ( a ) Control, PCR under the standard conditions described in Materials and Methods. ( b ) Similar to (a), but with Tth RecA protein (0.4 μg per 25 μl reaction mixture). ( c ) Similar to (a), but with ATP (400 μM). ( d ) Similar to (a), but with Tth RecA protein (0.4 μg per 25 μl reaction mixture) and ATP (300 μM). ( e ) Similar to (a), but with Tth RecA protein (0.4 μg per 25 μl reaction mixture) and ATP-γS (300 μM). The products were electrophoresed and stained with ethidium bromide. Molecular weight markers are indicated on the right-hand side of these panels. The oligonucleotide sequences used for the primers were as follows: 5′-ACAATGGGCTCACTCACCCA-3′ and 5′-CTAAGACCAATGGATAGCTG-3′ for lane 1 (300 bp); 5′-GCTCAGCATGGTGGTGGCAT-3′ and 5′-CCTCATACCTTCCCCCCCAT-3′ for lane 2 (319 bp); 5′-GACTACTCTAGCGACTGTCC-3′ and 5′-GACAGCCACCAGATCCAATC-3′ for lane 3 (360 bp); 5′-AACCTCACAACCTTGGCTGA-3′ and 5′-TTCACAACTTAAGATTTGGC-3′ for lane 4 (400 bp); 5′-AGGCAACTAGGATGGTGTGG-3′ and 5′-CAGGGAGCGTGTCCATAGGG-3′ for lane 5 (450 bp); 5′-CTGCTGAAAGAGATGCGGTG-3′ and 5′-AGGAAAACAGCCCAAGGGAC-3′ for lane 6 (469 bp); and 5′-ACTTTGTTCTGAGCCTCACA-3′ and 5′-GTTGCCCAATCGCCCCTCTC-3′ for lane 7 (650 bp).

    Article Snippet: The same results were obtained by the other polymerase systems: LA Taq polymerase (Takara-bio), Tth polymerase (Applied-boisystems); Expand High Fidelity, Expand High FidelityPLUS and Expand Long Template polymerase (Roche-diagnostics); TITANIUM Taq polymerase (Becton-Dickinson-Clontech); and Taq polymerase (Promega).

    Techniques: Polymerase Chain Reaction, Staining, Molecular Weight

    Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA Taq polymerase HS. M is the DNA ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region

    Journal: Heredity

    Article Title: Deficiency of a pyrroline-5-carboxylate reductase produces the yellowish green cocoon ‘Ryokuken’ of the silkworm, Bombyx mori

    doi: 10.1038/s41437-018-0051-8

    Figure Lengend Snippet: Comparison of the BmP5CR1 genomic region between Daizo (+ Lg /+ Lg ) and DH6 ( Lg / Lg ). ( a ) Genomic PCR analysis of the three parts of BmP5CR1 by Ex or LA Taq polymerase HS. M is the DNA ladder marker. ( b ) Schematic representation of the BmP5CR1 gene structure. Lines and rectangular boxes show introns and exons, respectively. White boxes indicate the untranslated regions (UTRs), and grey boxes are the coding regions. Arrows indicate differences between Daizo and DH6. Arrowheads are primer sites used in this study. Numbers show the length (bp) of each region

    Article Snippet: The BmP5CR1 -specific region was amplified from 10 ng of genomic DNA using 2.5 U Ex Taq or LA Taq HS polymerase (TaKaRa, Japan).

    Techniques: Polymerase Chain Reaction, Marker