la pcr buffer Takara Search Results


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  • 86
    TaKaRa takara la pcr buffer
    Effects of Tth MutS on standard polymerase chain reaction <t>(PCR)</t> amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. <t>aeolicus</t> DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    Takara La Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa takara la buffer
    Effects of Tth MutS on standard polymerase chain reaction <t>(PCR)</t> amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. <t>aeolicus</t> DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    Takara La Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa takara la taq dna polymerase with gc buffer
    Effects of Tth MutS on standard polymerase chain reaction <t>(PCR)</t> amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. <t>aeolicus</t> DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    Takara La Taq Dna Polymerase With Gc Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 84/100, based on 1321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq dna polymerase buffer
    Effects of Tth MutS on standard polymerase chain reaction <t>(PCR)</t> amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. <t>aeolicus</t> DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    La Taq Dna Polymerase Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pcr kit
    Effects of Tth MutS on standard polymerase chain reaction <t>(PCR)</t> amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. <t>aeolicus</t> DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1961 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq pcr buffer
    Effects of Tth MutS on standard polymerase chain reaction <t>(PCR)</t> amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. <t>aeolicus</t> DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    La Taq Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa 10×la pcr buffer
    Effects of Tth MutS on standard polymerase chain reaction <t>(PCR)</t> amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. <t>aeolicus</t> DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    10×La Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pcr mastermix
    Effects of Tth MutS on standard polymerase chain reaction <t>(PCR)</t> amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. <t>aeolicus</t> DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.
    Pcr Mastermix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la pcr buffer ll
    Variation in 3DPCR <t>Taq</t> polymerase background mutation rate across the <t>PCR</t> block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.
    La Pcr Buffer Ll, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq pcr solution
    Variation in 3DPCR <t>Taq</t> polymerase background mutation rate across the <t>PCR</t> block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.
    La Taq Pcr Solution, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa 10x la pcr buffer
    Variation in 3DPCR <t>Taq</t> polymerase background mutation rate across the <t>PCR</t> block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.
    10x La Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa rna pcr kit
    Variation in 3DPCR <t>Taq</t> polymerase background mutation rate across the <t>PCR</t> block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.
    Rna Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3430 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.

    Journal: International Journal of Molecular Sciences

    Article Title: Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR)

    doi: 10.3390/ijms14036436

    Figure Lengend Snippet: Effects of Tth MutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. ( A ) Schema tic representation of the primers and templates used in ( B , C ). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; ( B ) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; ( C ) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the Tth MutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM Tth MutS.

    Article Snippet: PCR was performed with 0.06 units/μL LA Taq Hot-Start Version (Takara, Shiga, Japan), 0.03 units/μL KOD polymerase (Toyobo, Tokyo, Japan) or 40 nM A. aeolicus DnaE, in 1× Takara LA PCR buffer II (Takara, Shiga, Japan) containing 20 nM template DNA; 400 nM primers; 400 μM dATP, dTTP, dCTP and dGTP and various concentrations of Tth MutS or Aae MutS.

    Techniques: Polymerase Chain Reaction, Amplification, Concentration Assay

    PCR products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, DNA marker DL 2000; lane M 2 , λ-Hind III DNA marker

    Journal: Archives of Medical Science : AMS

    Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems

    doi: 10.5114/aoms.2018.79005

    Figure Lengend Snippet: PCR products verifying the recombinant plasmids pFastBacHT A-Der f 1, pFastBacHT A-Der f 2, and pFast-BacHT A-Der f 4. Lanes 1–8, Der f 1; lanes 9–16, Der f 2; lanes 17–24, Der f 4; lane M1, DNA marker DL 2000; lane M 2 , λ-Hind III DNA marker

    Article Snippet: The reaction systems were as follows: 5 µl of 10 × LA PCR Buffer, 0.5 µl of TaKaRa Pyrobest DNA Polymerase, 8 µl of dNTP, 1 µl of forward primers, 1 µl of reverse primers, 0.5 µl of plasmids pET28a(+)-Der f 1/pET28a(+)-Der f 2/ pET28a(+)-Der f 4 (as appropriate), and 34 µl of ddH2 O; the total reaction volume was 50 µl.

    Techniques: Polymerase Chain Reaction, Recombinant, Marker

    Variation in 3DPCR Taq polymerase background mutation rate across the PCR block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.

    Journal: British Journal of Cancer

    Article Title: Erroneous identification of APOBEC3-edited chromosomal DNA in cancer genomics

    doi: 10.1038/bjc.2014.176

    Figure Lengend Snippet: Variation in 3DPCR Taq polymerase background mutation rate across the PCR block. ( A ) White spots correspond to PCR-positive samples for a 176 bp fragment of human TP53 . Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A10- and D8-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) Distribution of the number of CG- > TA transitions per clone. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible. ( E ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars.

    Article Snippet: The buffer conditions were 2.5 mM MgCl2 , 1 × LA PCR Buffer ll (TaKaRa LA Taq ; TaKaRa, Otsu, Japan), 400 μ M each deoxynucleoside triphosphate (TaKaRa LA Taq ; TaKaRa), 100 μ M each primer and 1.5 U of Taq (EurobioTaq+ Eurobio AbCys).

    Techniques: Mutagenesis, Polymerase Chain Reaction, Blocking Assay, Clone Assay, Derivative Assay

    Variation in 3DPCR long-range Taq polymerase background mutation rate across the PCR block. ( A ) White spots correspond to PCR-positive samples for TP53 DNA. Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A6-, B3- and C2-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible.

    Journal: British Journal of Cancer

    Article Title: Erroneous identification of APOBEC3-edited chromosomal DNA in cancer genomics

    doi: 10.1038/bjc.2014.176

    Figure Lengend Snippet: Variation in 3DPCR long-range Taq polymerase background mutation rate across the PCR block. ( A ) White spots correspond to PCR-positive samples for TP53 DNA. Those samples indicated by an asterisk were cloned and sequenced. ( B ) Mutation matrices for A6-, B3- and C2-derived sequences; transitions were invariably of the type N- > T,A; the number of bases sequenced is given by n. ( C ) 5′ Dinucleotide context for the G- > A and C- > T transitions, with the expected values shown as horizontal bars. ( D ) A collection of the most highly mutated sequences. To compact the data, only variable sites are shown, their positions being identified above. Nucleotide positions should be read from top to bottom. To the right are the numbers of mutations per clone (mut) and the minimum number of recombination events (rec) to explain the complexity. Zones of recombination are highlighted in grey shading when possible.

    Article Snippet: The buffer conditions were 2.5 mM MgCl2 , 1 × LA PCR Buffer ll (TaKaRa LA Taq ; TaKaRa, Otsu, Japan), 400 μ M each deoxynucleoside triphosphate (TaKaRa LA Taq ; TaKaRa), 100 μ M each primer and 1.5 U of Taq (EurobioTaq+ Eurobio AbCys).

    Techniques: Mutagenesis, Polymerase Chain Reaction, Blocking Assay, Clone Assay, Derivative Assay