l. monocytogenes strains Search Results


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  • 99
    ATCC l monocytogenes strains
    Inhibition of the growth of L. <t>monocytogenes</t> ATCC 7644 (A, C) and L. monocytogenes ATCC 19114 (B, D) by listeria phages in tryptic soy broth at 30°C.
    L Monocytogenes Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SYGNIS AG l monocytogenes strain egd e
    Genomic locations of the selected virulence loci in L. <t>monocytogenes</t> strain <t>EGD-e.</t> The six loci analyzed are shown in boldface characters.
    L Monocytogenes Strain Egd E, supplied by SYGNIS AG, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC strain l monocytogenes
    Application of model: comparison of simulated (Simu.) and observed CFU of L. <t>monocytogenes</t> strain <t>ATCC</t> 19115 in blue-white soft cheese when inoculated at day 13 (means and 95% confidence intervals). Applied parameter: μ opt = 0.43 h −1 .
    Strain L Monocytogenes, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC hemolytic l monocytogenes strain
    Application of model: comparison of simulated (Simu.) and observed CFU of L. <t>monocytogenes</t> strain <t>ATCC</t> 19115 in blue-white soft cheese when inoculated at day 13 (means and 95% confidence intervals). Applied parameter: μ opt = 0.43 h −1 .
    Hemolytic L Monocytogenes Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG l monocytogenes strains
    Relative transcript expression profiles of select genes from weakly-adherent (CW35) and strongly-adherent (99-38) strains of L. <t>monocytogenes</t> . Panel A, from cells recovered from planktonic growth at 30 °C. Panel B, from cells attached to glass beads during growth at 30 °C. Panel C, from planktonic cells grown at 42 °C. Expression is relative to that of the reference gene, 16S rRNA. All data bars represent the means of triplicate replications for gene expression RT-qPCR assays. Error bars indicate the standard deviation from the mean. Expression was normalized (×10 7 factor) to eliminate negative expression levels.
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    ATCC l monocytogenes strain 43251
    Relative transcript expression profiles of select genes from weakly-adherent (CW35) and strongly-adherent (99-38) strains of L. <t>monocytogenes</t> . Panel A, from cells recovered from planktonic growth at 30 °C. Panel B, from cells attached to glass beads during growth at 30 °C. Panel C, from planktonic cells grown at 42 °C. Expression is relative to that of the reference gene, 16S rRNA. All data bars represent the means of triplicate replications for gene expression RT-qPCR assays. Error bars indicate the standard deviation from the mean. Expression was normalized (×10 7 factor) to eliminate negative expression levels.
    L Monocytogenes Strain 43251, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC nonhemolytic l monocytogenes strain
    CPE in Caco-2 monolayers. CPE was assessed with trypan blue, which stains only dead cells. (A) Uninfected control; (B) E. faecium LS10; (C) L. <t>monocytogenes</t> ATCC 43248; (D) L. monocytogenes ATCC 43249. Magnification, ×100.
    Nonhemolytic L Monocytogenes Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC l monocytogenes strains 19112
    CPE in Caco-2 monolayers. CPE was assessed with trypan blue, which stains only dead cells. (A) Uninfected control; (B) E. faecium LS10; (C) L. <t>monocytogenes</t> ATCC 43248; (D) L. monocytogenes ATCC 43249. Magnification, ×100.
    L Monocytogenes Strains 19112, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega l monocytogenes strains
    Absolute number of Listeria <t>monocytogenes</t> meningitis cases for the largest multi locus sequence type groups from 1985 to 2014 in The Netherlands. The absolute number of cases per listerial multi-locus sequence type causing meningitis in the Netherlands is shown for six time intervals. The number of listeria meningitis cases did not vary significantly over the time intervals. Data for the five most common sequence types (STs) is shown. Remaining STs are clustered in the category ‘other STs’. In the time interval 1985–1989, ST1 and ST2 were the dominant STs. In the following years, ST1 and ST2 cases decreased whereas ST6 cases increased. In 2010–2014, ST6 caused disease in most cases, followed by ST1 and ST2. The number of ST6 cases increased significantly over the years (Mann–Whitney U test, p
    L Monocytogenes Strains, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC l monocytogenes atcc strain 13932
    Absolute number of Listeria <t>monocytogenes</t> meningitis cases for the largest multi locus sequence type groups from 1985 to 2014 in The Netherlands. The absolute number of cases per listerial multi-locus sequence type causing meningitis in the Netherlands is shown for six time intervals. The number of listeria meningitis cases did not vary significantly over the time intervals. Data for the five most common sequence types (STs) is shown. Remaining STs are clustered in the category ‘other STs’. In the time interval 1985–1989, ST1 and ST2 were the dominant STs. In the following years, ST1 and ST2 cases decreased whereas ST6 cases increased. In 2010–2014, ST6 caused disease in most cases, followed by ST1 and ST2. The number of ST6 cases increased significantly over the years (Mann–Whitney U test, p
    L Monocytogenes Atcc Strain 13932, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    scharlau strain l monocytogenes egde
    Absolute number of Listeria <t>monocytogenes</t> meningitis cases for the largest multi locus sequence type groups from 1985 to 2014 in The Netherlands. The absolute number of cases per listerial multi-locus sequence type causing meningitis in the Netherlands is shown for six time intervals. The number of listeria meningitis cases did not vary significantly over the time intervals. Data for the five most common sequence types (STs) is shown. Remaining STs are clustered in the category ‘other STs’. In the time interval 1985–1989, ST1 and ST2 were the dominant STs. In the following years, ST1 and ST2 cases decreased whereas ST6 cases increased. In 2010–2014, ST6 caused disease in most cases, followed by ST1 and ST2. The number of ST6 cases increased significantly over the years (Mann–Whitney U test, p
    Strain L Monocytogenes Egde, supplied by scharlau, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biotechnology Information l monocytogenes strain f2365
    Real-time PCR amplifications of serial dilutions of L. <t>monocytogenes</t> <t>F2365</t> DNA equivalent to 100,000 to 10 target molecules. Solid squares, 100,000 cell equivalents; solid circles, 10,000 cell equivalents; triangles, 1,000 cell equivalents; inverted triangles,
    L Monocytogenes Strain F2365, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC l monocytogenes strain atcc 15313
    Western blot analysis of extracellular proteins from different L. <t>monocytogenes</t> strains performed with a representative anti-PC-PLC MAb obtained in the present study. Lane 1, molecular weight markers; lane 2, ATCC 15313; lane 3, HCC23; lane 4, ATCC 19115; lane 5, HCC7; lane 6, CCF4; lane 7, EGD; lane 8, CCF1.
    L Monocytogenes Strain Atcc 15313, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Xenogen Corporation luminescent l monocytogenes strain xen 32
    Diminished clearance of L. <t>monocytogenes</t> in the distal small intestine of MyD88-deficient mice. (A) MyD88 +/+ and MyD88 −/− mice were infected with 10 10 L. monocytogenes by gavage, and CFUs in the proximal (pro) and distal part of the small intestinal wall were determined 24 h p.i.; n = 8 mice/group. (B) Segments from proximal (pro) or distal small intestine were cultured in media containing 2,000 L. monocytogenes. After 4 h, bacterial burden in the supernatant was determined. Values are representative of two individual experiments. (C) Schematic representation of the in vivo luminal killing assay. (D) Bioluminescence imaging of luminescent L. monocytogenes in ileal loops of MyD88 +/+ and MyD88 −/− mice. Luminescent L. monocytogenes (2.5 × 10 5 ) was injected into ileal loops of MyD88 +/+ and MyD88 −/− mice and imaged 30 min (top) and 2 h (bottom) after injection with an IVIS Imaging System. One representative mouse per group is shown. n = 5. (E) 1,000 L. monocytogenes were injected into ileal loops of wt, MyD88 −/− , TNF −/− /IFN-γ −/− , and Rip2 −/− mice. 2 h after injection, the isolated section of the intestine was harvested and CFUs in the luminal fluid were detected. n = 3 for MyD88 −/− ; n = 5–9 for all other groups. Bacterial numbers are expressed as the percentage of recovered L. monocytogenes . *, P ≤ 0.05, ***, P
    Luminescent L Monocytogenes Strain Xen 32, supplied by Xenogen Corporation, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC l monocytogenes strain atcc 35152
    Diminished clearance of L. <t>monocytogenes</t> in the distal small intestine of MyD88-deficient mice. (A) MyD88 +/+ and MyD88 −/− mice were infected with 10 10 L. monocytogenes by gavage, and CFUs in the proximal (pro) and distal part of the small intestinal wall were determined 24 h p.i.; n = 8 mice/group. (B) Segments from proximal (pro) or distal small intestine were cultured in media containing 2,000 L. monocytogenes. After 4 h, bacterial burden in the supernatant was determined. Values are representative of two individual experiments. (C) Schematic representation of the in vivo luminal killing assay. (D) Bioluminescence imaging of luminescent L. monocytogenes in ileal loops of MyD88 +/+ and MyD88 −/− mice. Luminescent L. monocytogenes (2.5 × 10 5 ) was injected into ileal loops of MyD88 +/+ and MyD88 −/− mice and imaged 30 min (top) and 2 h (bottom) after injection with an IVIS Imaging System. One representative mouse per group is shown. n = 5. (E) 1,000 L. monocytogenes were injected into ileal loops of wt, MyD88 −/− , TNF −/− /IFN-γ −/− , and Rip2 −/− mice. 2 h after injection, the isolated section of the intestine was harvested and CFUs in the luminal fluid were detected. n = 3 for MyD88 −/− ; n = 5–9 for all other groups. Bacterial numbers are expressed as the percentage of recovered L. monocytogenes . *, P ≤ 0.05, ***, P
    L Monocytogenes Strain Atcc 35152, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC indicator strain l monocytogenes atcc 19117
    Fluorescence scanning microscopy analysis of L . <t>monocytogenes</t> <t>ATCC</t> 19117 cells without (a) and treated with bacteriocin BacFL31 for 15 min (b), 30 min (c), and 1 h (d).
    Indicator Strain L Monocytogenes Atcc 19117, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC three strain l monocytogenes cocktail
    The effect of live and heat-killed Enterococcus faecium BGPAS1-3 on (A) adhesion and (B) invasion of Listeria <t>monocytogenes</t> <t>ATCC</t> 19111 on differentiated Caco-2 cells were analyzed in the competitive, the displacement and the exclusion assays. Three experiments were done. One-way ANOVA with the Tukey’s post hoc test was used to compare CFU of L. monocytogenes ATCC 19111 in cultures with and without BGPAS1-3. Statistical significance p
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    ATCC l monocytogenes strain atcc
    Molecular weight estimation of the Leuconostoc lactis SD501 bacteriocin. (A) Tricine SDS-PAGE gel stained with Coomassie Brilliant Blue G250 for protein detection. (B) Tricine gel overlaid with soft agar containing Listeria <t>monocytogenes</t> ATCC 19114: lanes 1 and 3, Precision Plus Protein™ Dual Xtra Prestained Protein Standards marker; lanes 2 and 4, purified bacteriocin from Leuc. lactis SD501.
    L Monocytogenes Strain Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC l monocytogenes reference strain atcc 51775
    Molecular weight estimation of the Leuconostoc lactis SD501 bacteriocin. (A) Tricine SDS-PAGE gel stained with Coomassie Brilliant Blue G250 for protein detection. (B) Tricine gel overlaid with soft agar containing Listeria <t>monocytogenes</t> ATCC 19114: lanes 1 and 3, Precision Plus Protein™ Dual Xtra Prestained Protein Standards marker; lanes 2 and 4, purified bacteriocin from Leuc. lactis SD501.
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    Biotechnology Information l monocytogenes strain egd e
    Molecular weight estimation of the Leuconostoc lactis SD501 bacteriocin. (A) Tricine SDS-PAGE gel stained with Coomassie Brilliant Blue G250 for protein detection. (B) Tricine gel overlaid with soft agar containing Listeria <t>monocytogenes</t> ATCC 19114: lanes 1 and 3, Precision Plus Protein™ Dual Xtra Prestained Protein Standards marker; lanes 2 and 4, purified bacteriocin from Leuc. lactis SD501.
    L Monocytogenes Strain Egd E, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Celldex recombinant l monocytogenes strains
    Molecular weight estimation of the Leuconostoc lactis SD501 bacteriocin. (A) Tricine SDS-PAGE gel stained with Coomassie Brilliant Blue G250 for protein detection. (B) Tricine gel overlaid with soft agar containing Listeria <t>monocytogenes</t> ATCC 19114: lanes 1 and 3, Precision Plus Protein™ Dual Xtra Prestained Protein Standards marker; lanes 2 and 4, purified bacteriocin from Leuc. lactis SD501.
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    ATCC l monocytogenes type strain egde
    Genomic organization of the hypervariable hotspot 9 region in L. <t>monocytogenes</t> genomes harbouring homologues to proteins from plasmid pLMIV of L. monocytogenes FSL J1–208. Homologous proteins are shown as the same color. pLMIV and L. monocytogenes <t>EGDe</t> locus_tags are indicated.
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    Aduro tagbfp expressing l monocytogenes strain
    Genomic organization of the hypervariable hotspot 9 region in L. <t>monocytogenes</t> genomes harbouring homologues to proteins from plasmid pLMIV of L. monocytogenes FSL J1–208. Homologous proteins are shown as the same color. pLMIV and L. monocytogenes <t>EGDe</t> locus_tags are indicated.
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    ATCC l monocytogenes strain 33116 atcc 19117
    Genomic organization of the hypervariable hotspot 9 region in L. <t>monocytogenes</t> genomes harbouring homologues to proteins from plasmid pLMIV of L. monocytogenes FSL J1–208. Homologous proteins are shown as the same color. pLMIV and L. monocytogenes <t>EGDe</t> locus_tags are indicated.
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    Biotechnology Information l monocytogenes strain egd e chromosome sequence
    Comparison of consensus sequences calculated from alignments of Illumina MiSeq short-read data to a non-identical reference with four reference-guided assemblers. Genomic DNA from the Listeriosis Reference Service for Canada's (LRS) Listeria <t>monocytogenes</t> strain 08-5578 subculture was sequenced and indexed twelve times. The resulting reads were aligned with four reference-guided assemblers (BWA, MOSAIK, Novoalign, and SMALT) using an L. monocytogenes strain <t>EGD-e</t> chromosome sequence obtained from the National Center for Biotechnology Information (NCBI) archive as a reference. The NCBI strain EGD-e chromosome sequence differs from the LRS strain 08-5578 chromosome sequence at 25,347 nucleotide positions. The numbers of false positive sites (a), true positive sites (b), ambiguous sites (c), and gaps (d) present in the resulting consensus sequences relative to the calculated coverage of each assembly are shown. Lines were calculated with power regression and smoothed using a Catmull-Rom Spline.
    L Monocytogenes Strain Egd E Chromosome Sequence, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biotechnology Information l monocytogenes strain 08 5578 chromosome sequence
    Comparison of consensus sequences calculated from assemblies of simulated Illumina short-read data aligned to references of different genetic distances with four reference-guided assemblers. Ten sets of simulated sequencing reads were generated using a Listeria <t>monocytogenes</t> strain 08-5578 chromosome sequence obtained from the National Center for Biotechnology Information archive as a reference. Nucleotide variants were randomly introduced (10 1 –10 5 ) in silico to the 08-5578 chromosome sequence to simulate the presence of SNPs in five reference sequences. The performance of four reference-guided short-read sequence assemblers (BWA, MOSAIK, Novoalign, and SMALT) was assessed by averaging the percentages of true SNPs detected (a) and the numbers of gaps present (b) in the consensus sequences generated from alignments of the ten sets of reads. In addition, average assembly processing times are provided (c).
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    Illumina Inc l monocytogenes strain 08 5578 illumina short read sequence datasets
    Comparison of consensus sequences calculated from assemblies of simulated Illumina short-read data aligned to references of different genetic distances with four reference-guided assemblers. Ten sets of simulated sequencing reads were generated using a Listeria <t>monocytogenes</t> strain 08-5578 chromosome sequence obtained from the National Center for Biotechnology Information archive as a reference. Nucleotide variants were randomly introduced (10 1 –10 5 ) in silico to the 08-5578 chromosome sequence to simulate the presence of SNPs in five reference sequences. The performance of four reference-guided short-read sequence assemblers (BWA, MOSAIK, Novoalign, and SMALT) was assessed by averaging the percentages of true SNPs detected (a) and the numbers of gaps present (b) in the consensus sequences generated from alignments of the ten sets of reads. In addition, average assembly processing times are provided (c).
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    91
    Selleck Chemicals kinase inhibitor libraries against wild type l monocytogenes strain 10403s
    Comparison of consensus sequences calculated from assemblies of simulated Illumina short-read data aligned to references of different genetic distances with four reference-guided assemblers. Ten sets of simulated sequencing reads were generated using a Listeria <t>monocytogenes</t> strain 08-5578 chromosome sequence obtained from the National Center for Biotechnology Information archive as a reference. Nucleotide variants were randomly introduced (10 1 –10 5 ) in silico to the 08-5578 chromosome sequence to simulate the presence of SNPs in five reference sequences. The performance of four reference-guided short-read sequence assemblers (BWA, MOSAIK, Novoalign, and SMALT) was assessed by averaging the percentages of true SNPs detected (a) and the numbers of gaps present (b) in the consensus sequences generated from alignments of the ten sets of reads. In addition, average assembly processing times are provided (c).
    Kinase Inhibitor Libraries Against Wild Type L Monocytogenes Strain 10403s, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC l monocytogenes strain atcc 7644
    Comparison of consensus sequences calculated from assemblies of simulated Illumina short-read data aligned to references of different genetic distances with four reference-guided assemblers. Ten sets of simulated sequencing reads were generated using a Listeria <t>monocytogenes</t> strain 08-5578 chromosome sequence obtained from the National Center for Biotechnology Information archive as a reference. Nucleotide variants were randomly introduced (10 1 –10 5 ) in silico to the 08-5578 chromosome sequence to simulate the presence of SNPs in five reference sequences. The performance of four reference-guided short-read sequence assemblers (BWA, MOSAIK, Novoalign, and SMALT) was assessed by averaging the percentages of true SNPs detected (a) and the numbers of gaps present (b) in the consensus sequences generated from alignments of the ten sets of reads. In addition, average assembly processing times are provided (c).
    L Monocytogenes Strain Atcc 7644, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Xenogen Corporation l monocytogenes
    Rescue of the elektra phenotype by BAC transgenesis Transgenic mice were produced by microinjecting a BAC clone containing the wild-type Slfn2 sequence into single-cell embryos homozygous for the elektra mutation. (a) DNA sequence of the BAC clone and genomic DNA from an elektra homozygote in the region of the Slfn2 elektra mutation. The BAC clone contains the wild-type Slfn2 sequence. (b) Flow cytometric analysis of CD8 and CD4 staining of blood from a WT mouse, a homozygous elektra transgenic mouse carrying the Slfn2 transgene ( elektra-BAC Tg ), and a littermate lacking the transgene ( elektra ). Results are representative of 4 mice per genotype. (c) Survival curves for transgenic mice ( elektra-BAC Tg , n =7), littermates without the transgene ( elektra , n =4), and WT mice ( n =5) upon challenge with 2 × 10 5 PFU of MCMV. (d) Survival curves for transgenic mice ( elektra-BAC Tg , n =7), littermates without the transgene ( elektra , n =5), and WT mice ( n =7) upon challenge with 5 × 10 5 CFU of L. <t>monocytogenes</t> .
    L Monocytogenes, supplied by Xenogen Corporation, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Difco l monocytogenes
    Septin expression, localization, and recruitment at the bacterial entry site in human non-phagocytic cells. (A) Western blots (WB) of septins in non-phagocytic cell lines. HeLa and JEG-3 cells were harvested, and cell lysates were separated by 10% SDS-PAGE before immunoblotting. Blots were probed with antibodies specific to GAPDH, SEPT2, SEPT9, SEPT11, and actin. Blots for GAPDH are shown as a loading control. (B) Septin filaments partially colocalize with actin filaments in HeLa cells. Endogenous α-tubulin, F-actin, and SEPT2, SEPT9, or SEPT11 were visualized by immunostaining with anti-α-tubulin (grey and in Merge blue), anti-F-actin (green), and anti-SEPT2, anti-SEPT9, or anti-SEPT11 antibodies (red). Representative confocal images for α-tubulin, F-actin, and SEPT11 distribution in HeLa cells are here displayed, and similar images were obtained labeling for SEPT2 or SEPT9. Scale bars indicate 10 µm. (C) Septin filaments partially colocalize with microtubules in JEG-3 cells. Endogenous α–tubulin, F-actin, and SEPT2, SEPT9, or SEPT11 were visualized for immunostaining with anti-α-tubulin (grey and in Merge blue), anti-F-actin (green), and anti-SEPT2, anti-SEPT9, or anti-SEPT11 antibodies (red). Representative confocal images for α-tubulin, F-actin, and SEPT11 distribution in JEG-3 cells are here displayed, and similar images were obtained labeling for SEPT2 or SEPT9. Scale bars indicate 10 µm. (D) Septin recruitment at the site of Listeria entry in JEG-3 cells. Cells were infected with L. <t>monocytogenes</t> BUG 1641 for 5, 10, or 15 minutes and then fixed for microscopy. Endogenous septin was stained with anti-SEPT2, anti-SEPT9, or anti-SEPT11 antibodies (red). Actin was stained with anti-F-actin (green), and Listeria was marked using DAPI (blue). Representative photos are here displayed, where inset images highlight the septin collar-like recruitment around Listeria to which the white arrows are pointing. Scale bars indicate 1 µm. (E, F) Septin recruitment to the site of Shigella entry in (E) JEG-3 cells or (F) HeLa cells. Cells were infected with S. flexneri M90T for 15 minutes and then fixed for microscopy. Endogenous septin was stained with anti-SEPT2, anti-SEPT9, or anti-SEPT11 antibodies (red). Actin was stained with anti-F-actin (green), and Shigella was marked using DAPI (blue). Representative photos showing SEPT11 (JEG-3 cells) and SEPT2 (HeLa cells) are here displayed, where inset images highlight the septin collar-like recruitment around Shigella to which the white arrows are pointing. In JEG-3 cell and HeLa cells, similar recruitment was obtained when labeling for SEPT2, SEPT9, or SEPT11. Scale bars indicate 1 µm.
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    Image Search Results


    Inhibition of the growth of L. monocytogenes ATCC 7644 (A, C) and L. monocytogenes ATCC 19114 (B, D) by listeria phages in tryptic soy broth at 30°C.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Isolation and Characterization of Listeria phages for Control of Growth of Listeria monocytogenes in Milk

    doi: 10.5851/kosfa.2017.37.2.320

    Figure Lengend Snippet: Inhibition of the growth of L. monocytogenes ATCC 7644 (A, C) and L. monocytogenes ATCC 19114 (B, D) by listeria phages in tryptic soy broth at 30°C.

    Article Snippet: However, high lytic activity of the isolated bacteriophages against the four different serotypes of L. monocytogenes ( ) was confirmed by soft agar overlay, suggesting a potential use of these phages against a wide range of L. monocytogenes strains.

    Techniques: Inhibition

    Transmission electron microphotograph of the phage LMP1 targeting Listeria monocytogenes .

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Isolation and Characterization of Listeria phages for Control of Growth of Listeria monocytogenes in Milk

    doi: 10.5851/kosfa.2017.37.2.320

    Figure Lengend Snippet: Transmission electron microphotograph of the phage LMP1 targeting Listeria monocytogenes .

    Article Snippet: However, high lytic activity of the isolated bacteriophages against the four different serotypes of L. monocytogenes ( ) was confirmed by soft agar overlay, suggesting a potential use of these phages against a wide range of L. monocytogenes strains.

    Techniques: Transmission Assay

    Inhibition of the growth of L. monocytogenes ATCC 7644 (A) and L. monocytogenes ATCC 19114 (B) by listeria phages in tryptic soy broth at 10°C for 5 d.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Isolation and Characterization of Listeria phages for Control of Growth of Listeria monocytogenes in Milk

    doi: 10.5851/kosfa.2017.37.2.320

    Figure Lengend Snippet: Inhibition of the growth of L. monocytogenes ATCC 7644 (A) and L. monocytogenes ATCC 19114 (B) by listeria phages in tryptic soy broth at 10°C for 5 d.

    Article Snippet: However, high lytic activity of the isolated bacteriophages against the four different serotypes of L. monocytogenes ( ) was confirmed by soft agar overlay, suggesting a potential use of these phages against a wide range of L. monocytogenes strains.

    Techniques: Inhibition

    Inhibition of the growth of L. monocytogenes ATCC 7644 (A, C) and L. monocytogenes ATCC 19114 (B, D) by listeria phages in milk media at 10°C and 4°C.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Isolation and Characterization of Listeria phages for Control of Growth of Listeria monocytogenes in Milk

    doi: 10.5851/kosfa.2017.37.2.320

    Figure Lengend Snippet: Inhibition of the growth of L. monocytogenes ATCC 7644 (A, C) and L. monocytogenes ATCC 19114 (B, D) by listeria phages in milk media at 10°C and 4°C.

    Article Snippet: However, high lytic activity of the isolated bacteriophages against the four different serotypes of L. monocytogenes ( ) was confirmed by soft agar overlay, suggesting a potential use of these phages against a wide range of L. monocytogenes strains.

    Techniques: Inhibition

    Inhibition of the growth of L. monocytogenes ATCC 7644 (A) and L. monocytogenes ATCC 19114 (B) by listeria phages in milk media at 30°C.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Isolation and Characterization of Listeria phages for Control of Growth of Listeria monocytogenes in Milk

    doi: 10.5851/kosfa.2017.37.2.320

    Figure Lengend Snippet: Inhibition of the growth of L. monocytogenes ATCC 7644 (A) and L. monocytogenes ATCC 19114 (B) by listeria phages in milk media at 30°C.

    Article Snippet: However, high lytic activity of the isolated bacteriophages against the four different serotypes of L. monocytogenes ( ) was confirmed by soft agar overlay, suggesting a potential use of these phages against a wide range of L. monocytogenes strains.

    Techniques: Inhibition

    Detection of Listeria spp. strains employing the combined cell wall-binding domain-based magnetic separation (CBD-MS)/A511:: luxAB assay. Dilutions of pure cultures of five different Listeria strains representing different serovars were used for evaluating the combined CBD-MS/A511:: luxAB assay. ( a ) L. monocytogenes ScottA (serovar [SV] 4b); ( b ) L. monocytogenes EGDe (SV 1/2a); ( c ) L. monocytogenes WSLC 1001 (SV 1/2c); ( d ) L. ivanovii WSLC 3009 (SV 5); ( e ) L. innocua WSLC 2012 (SV 6b). The dotted horizontal bar indicates the level of background light emission (noise) of the assay, determined in the negative control sample (no bacteria). Asterisks indicate significant differences between values obtained from samples with bacteria and the respective control values. ****, p

    Journal: Viruses

    Article Title: Ultrasensitive and Fast Diagnostics of Viable Listeria Cells by CBD Magnetic Separation Combined with A511::luxAB Detection

    doi: 10.3390/v10110626

    Figure Lengend Snippet: Detection of Listeria spp. strains employing the combined cell wall-binding domain-based magnetic separation (CBD-MS)/A511:: luxAB assay. Dilutions of pure cultures of five different Listeria strains representing different serovars were used for evaluating the combined CBD-MS/A511:: luxAB assay. ( a ) L. monocytogenes ScottA (serovar [SV] 4b); ( b ) L. monocytogenes EGDe (SV 1/2a); ( c ) L. monocytogenes WSLC 1001 (SV 1/2c); ( d ) L. ivanovii WSLC 3009 (SV 5); ( e ) L. innocua WSLC 2012 (SV 6b). The dotted horizontal bar indicates the level of background light emission (noise) of the assay, determined in the negative control sample (no bacteria). Asterisks indicate significant differences between values obtained from samples with bacteria and the respective control values. ****, p

    Article Snippet: L. monocytogenes strains EGDe (serovar 1/2 a), WSLC 1001 (ATCC 19112) (sv 1/2c), WSLC 1685 (ScottA) (sv 4b), WSLC 2012 (ATCC 33091) (sv 6b), and Listeria ivanovii WSLC 3009 (sv 5) were grown in half-concentrated brain heart infusion medium (BHI ½) (Oxoid, Hampshire, UK), at 30 °C.

    Techniques: Binding Assay, Mass Spectrometry, Negative Control

    Detection of different concentrations of cells of L. monocytogenes ScottA and L. monocytogenes EGDe, in artificially contaminated food samples with the CBD-MS/A511:: luxAB assay. Eight samples for each food item were spiked with bacteria of either ScottA or EGDe strains, at contamination levels of 0.1, 1.0, 10.0, or 100.0 cfu/g. Additionally, one blank sample for each food item was prepared by adding sterile buffer without bacteria. All samples were tested for the presence of Listeria spp. in parallel. Error bars represent SD from two replicates. Asterisks indicate significant differences between values obtained from samples contaminated with bacteria and the respective blank samples. ****, p

    Journal: Viruses

    Article Title: Ultrasensitive and Fast Diagnostics of Viable Listeria Cells by CBD Magnetic Separation Combined with A511::luxAB Detection

    doi: 10.3390/v10110626

    Figure Lengend Snippet: Detection of different concentrations of cells of L. monocytogenes ScottA and L. monocytogenes EGDe, in artificially contaminated food samples with the CBD-MS/A511:: luxAB assay. Eight samples for each food item were spiked with bacteria of either ScottA or EGDe strains, at contamination levels of 0.1, 1.0, 10.0, or 100.0 cfu/g. Additionally, one blank sample for each food item was prepared by adding sterile buffer without bacteria. All samples were tested for the presence of Listeria spp. in parallel. Error bars represent SD from two replicates. Asterisks indicate significant differences between values obtained from samples contaminated with bacteria and the respective blank samples. ****, p

    Article Snippet: L. monocytogenes strains EGDe (serovar 1/2 a), WSLC 1001 (ATCC 19112) (sv 1/2c), WSLC 1685 (ScottA) (sv 4b), WSLC 2012 (ATCC 33091) (sv 6b), and Listeria ivanovii WSLC 3009 (sv 5) were grown in half-concentrated brain heart infusion medium (BHI ½) (Oxoid, Hampshire, UK), at 30 °C.

    Techniques: Mass Spectrometry

    Concentration-effect relationships for CEM-101 and azithromycin toward intraphagocytic L. monocytogenes (strain EGD, left panels) and L. pneumophila (strain ATCC 33153, right panels). The ordinate shows the change in CFU (Δ log CFU) per mg of

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Cellular Accumulation and Pharmacodynamic Evaluation of the Intracellular Activity of CEM-101, a Novel Fluoroketolide, against Staphylococcus aureus, Listeria monocytogenes, and Legionella pneumophila in Human THP-1 Macrophages

    doi: 10.1128/AAC.00203-09

    Figure Lengend Snippet: Concentration-effect relationships for CEM-101 and azithromycin toward intraphagocytic L. monocytogenes (strain EGD, left panels) and L. pneumophila (strain ATCC 33153, right panels). The ordinate shows the change in CFU (Δ log CFU) per mg of

    Article Snippet: S. aureus ATCC 25923 (methicillin [meticillin] sensitive), L. monocytogenes strain EGD, and L. pneumophila strain ATCC 33153 were used in the present study.

    Techniques: Concentration Assay

    Comparative susceptibilities of S. aureus ATCC 25923 and L. monocytogenes EGD to CEM-101, telithromycin, azithromycin, and clarithromycin, based on MIC determinations in pH-adjusted broth.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Cellular Accumulation and Pharmacodynamic Evaluation of the Intracellular Activity of CEM-101, a Novel Fluoroketolide, against Staphylococcus aureus, Listeria monocytogenes, and Legionella pneumophila in Human THP-1 Macrophages

    doi: 10.1128/AAC.00203-09

    Figure Lengend Snippet: Comparative susceptibilities of S. aureus ATCC 25923 and L. monocytogenes EGD to CEM-101, telithromycin, azithromycin, and clarithromycin, based on MIC determinations in pH-adjusted broth.

    Article Snippet: S. aureus ATCC 25923 (methicillin [meticillin] sensitive), L. monocytogenes strain EGD, and L. pneumophila strain ATCC 33153 were used in the present study.

    Techniques:

    Gene replacement of vip . ( A ) RT–PCR on total RNAs from L. monocytogenes WT and Δ vip strains using specific primers of vip and lmo0319 . Fragments ( vip and 0319 ) amplified are visualised. RT–PCR was performed on L. monocytogenes Δ vip + vip strains to confirm vip expression in the complemented strain. ( B ) Growth curves in BHI at 37°C.

    Journal: The EMBO Journal

    Article Title: Gp96 is a receptor for a novel Listeria monocytogenes virulence factor, Vip, a surface protein

    doi: 10.1038/sj.emboj.7600750

    Figure Lengend Snippet: Gene replacement of vip . ( A ) RT–PCR on total RNAs from L. monocytogenes WT and Δ vip strains using specific primers of vip and lmo0319 . Fragments ( vip and 0319 ) amplified are visualised. RT–PCR was performed on L. monocytogenes Δ vip + vip strains to confirm vip expression in the complemented strain. ( B ) Growth curves in BHI at 37°C.

    Article Snippet: We used the following L. monocytogenes isogenic strains: EGDe (WT, ATCC BAA-679), EGDeΔ prfA (gift from Dr M Kuhn), EGDeΔ inlA2 , EGDeΔ srtA and Δ srtB ( ).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Expressing

    Saturation of bacterial Vip or cellular Gp96 blocks L. monocytogenes invasion. ( A ) Pretreatment of cells with anti-Gp96 or Vip blocks Listeria entry. L2071 cells were pretreated with 5 or 25 μg/ml of anti-Gp96 or purified Vip and used for gentamicin assays with L. monocytogenes . Entry of Listeria into treated cells was compared to those into nontreated cells (NT), or cells treated with an equivalent concentration of rat-IgG or rabbit preimmune serum (preim) as control. ( B ) Pretreatment of Listeria with Gp96 or anti-Vip blocks entry into cells. Bacteria were preincubated with 25 μg/ml of purified Gp96 or anti-Vip and used for gentamicin assays on L2071 cells. Entry of treated bacteria was compared to that of nontreated bacteria (NT), or bacteria treated with an equivalent concentration of rat-IgG or rabbit preimmune serum (preim) as control. Values are given relative to the invasion of the WT strain arbitrarily fixed to 100. Experiments were repeated three times in duplicate.

    Journal: The EMBO Journal

    Article Title: Gp96 is a receptor for a novel Listeria monocytogenes virulence factor, Vip, a surface protein

    doi: 10.1038/sj.emboj.7600750

    Figure Lengend Snippet: Saturation of bacterial Vip or cellular Gp96 blocks L. monocytogenes invasion. ( A ) Pretreatment of cells with anti-Gp96 or Vip blocks Listeria entry. L2071 cells were pretreated with 5 or 25 μg/ml of anti-Gp96 or purified Vip and used for gentamicin assays with L. monocytogenes . Entry of Listeria into treated cells was compared to those into nontreated cells (NT), or cells treated with an equivalent concentration of rat-IgG or rabbit preimmune serum (preim) as control. ( B ) Pretreatment of Listeria with Gp96 or anti-Vip blocks entry into cells. Bacteria were preincubated with 25 μg/ml of purified Gp96 or anti-Vip and used for gentamicin assays on L2071 cells. Entry of treated bacteria was compared to that of nontreated bacteria (NT), or bacteria treated with an equivalent concentration of rat-IgG or rabbit preimmune serum (preim) as control. Values are given relative to the invasion of the WT strain arbitrarily fixed to 100. Experiments were repeated three times in duplicate.

    Article Snippet: We used the following L. monocytogenes isogenic strains: EGDe (WT, ATCC BAA-679), EGDeΔ prfA (gift from Dr M Kuhn), EGDeΔ inlA2 , EGDeΔ srtA and Δ srtB ( ).

    Techniques: Purification, Concentration Assay

    Overexpression of Gp96 enhances Listeria invasion. Entry of L. monocytogenes WT and Δ vip strains was analysed by gentamicin assay in L2071 cells and L2071 cells transfected with the pcDNA3 vector containing gp96 cDNA. Values are given relative to the invasion of the WT strain into L2071 cells arbitrarily fixed to 100. Experiments were repeated three times in duplicate for each cell line.

    Journal: The EMBO Journal

    Article Title: Gp96 is a receptor for a novel Listeria monocytogenes virulence factor, Vip, a surface protein

    doi: 10.1038/sj.emboj.7600750

    Figure Lengend Snippet: Overexpression of Gp96 enhances Listeria invasion. Entry of L. monocytogenes WT and Δ vip strains was analysed by gentamicin assay in L2071 cells and L2071 cells transfected with the pcDNA3 vector containing gp96 cDNA. Values are given relative to the invasion of the WT strain into L2071 cells arbitrarily fixed to 100. Experiments were repeated three times in duplicate for each cell line.

    Article Snippet: We used the following L. monocytogenes isogenic strains: EGDe (WT, ATCC BAA-679), EGDeΔ prfA (gift from Dr M Kuhn), EGDeΔ inlA2 , EGDeΔ srtA and Δ srtB ( ).

    Techniques: Over Expression, Transfection, Plasmid Preparation

    vip is PrfA regulated. ( A ) RT–PCRs of RNAs from logarithmic cultures in BHI at 37°C of L. monocytogenes WT and Δ prfA strains. The hly gene was used as control PrfA-regulated gene and the PrfA-independent iap gene to control RNA amounts. ( B ) Northern blot analysis of RNAs isolated from logarithmic cultures in BHI at 37°C of L. monocytogenes WT and Δ prfA strains. rRNAs were used to control RNA amounts.

    Journal: The EMBO Journal

    Article Title: Gp96 is a receptor for a novel Listeria monocytogenes virulence factor, Vip, a surface protein

    doi: 10.1038/sj.emboj.7600750

    Figure Lengend Snippet: vip is PrfA regulated. ( A ) RT–PCRs of RNAs from logarithmic cultures in BHI at 37°C of L. monocytogenes WT and Δ prfA strains. The hly gene was used as control PrfA-regulated gene and the PrfA-independent iap gene to control RNA amounts. ( B ) Northern blot analysis of RNAs isolated from logarithmic cultures in BHI at 37°C of L. monocytogenes WT and Δ prfA strains. rRNAs were used to control RNA amounts.

    Article Snippet: We used the following L. monocytogenes isogenic strains: EGDe (WT, ATCC BAA-679), EGDeΔ prfA (gift from Dr M Kuhn), EGDeΔ inlA2 , EGDeΔ srtA and Δ srtB ( ).

    Techniques: Northern Blot, Isolation

    Role of Vip in Listeria entry. ( A ) Entry of L. monocytogenes WT, Δ vip , Δ inlA and Δ vip + vip strains into Caco-2, GPC16, L2071 and Vero cells. After a 1 h infection, invasion values were calculated from the number of bacteria that survived to 2 h gentamicin incubation. Values are given relative to the invasion of the WT strain arbitrarily fixed to 100. ( B ) Entry and intracellular behaviour of the WT, Δ vip and Δ vip + vip strains in L2071 cells. Experiments were repeated three times in duplicate for each cell line.

    Journal: The EMBO Journal

    Article Title: Gp96 is a receptor for a novel Listeria monocytogenes virulence factor, Vip, a surface protein

    doi: 10.1038/sj.emboj.7600750

    Figure Lengend Snippet: Role of Vip in Listeria entry. ( A ) Entry of L. monocytogenes WT, Δ vip , Δ inlA and Δ vip + vip strains into Caco-2, GPC16, L2071 and Vero cells. After a 1 h infection, invasion values were calculated from the number of bacteria that survived to 2 h gentamicin incubation. Values are given relative to the invasion of the WT strain arbitrarily fixed to 100. ( B ) Entry and intracellular behaviour of the WT, Δ vip and Δ vip + vip strains in L2071 cells. Experiments were repeated three times in duplicate for each cell line.

    Article Snippet: We used the following L. monocytogenes isogenic strains: EGDe (WT, ATCC BAA-679), EGDeΔ prfA (gift from Dr M Kuhn), EGDeΔ inlA2 , EGDeΔ srtA and Δ srtB ( ).

    Techniques: Infection, Incubation

    Vip is a surface protein anchored to the bacterial cell wall by SrtA. Morphology of the vip mutant and display of Vip and InlA on the bacterial surface of L. monocytogenes WT, Δ vip , Δ vip + vip , Δ srtA and Δ srtB strains were analysed by immunofluorescence staining and confocal microscopy using antibodies against Vip and InlA.

    Journal: The EMBO Journal

    Article Title: Gp96 is a receptor for a novel Listeria monocytogenes virulence factor, Vip, a surface protein

    doi: 10.1038/sj.emboj.7600750

    Figure Lengend Snippet: Vip is a surface protein anchored to the bacterial cell wall by SrtA. Morphology of the vip mutant and display of Vip and InlA on the bacterial surface of L. monocytogenes WT, Δ vip , Δ vip + vip , Δ srtA and Δ srtB strains were analysed by immunofluorescence staining and confocal microscopy using antibodies against Vip and InlA.

    Article Snippet: We used the following L. monocytogenes isogenic strains: EGDe (WT, ATCC BAA-679), EGDeΔ prfA (gift from Dr M Kuhn), EGDeΔ inlA2 , EGDeΔ srtA and Δ srtB ( ).

    Techniques: Mutagenesis, Immunofluorescence, Staining, Confocal Microscopy

    ( A ) Amino-acid sequence of the vip product. Sp=signal peptide. ( B ) Genomic organisation of the vip region in L. monocytogenes and comparison with the homologous region in L. innocua . The arrows indicate gene orientation and hairpins putative terminators.

    Journal: The EMBO Journal

    Article Title: Gp96 is a receptor for a novel Listeria monocytogenes virulence factor, Vip, a surface protein

    doi: 10.1038/sj.emboj.7600750

    Figure Lengend Snippet: ( A ) Amino-acid sequence of the vip product. Sp=signal peptide. ( B ) Genomic organisation of the vip region in L. monocytogenes and comparison with the homologous region in L. innocua . The arrows indicate gene orientation and hairpins putative terminators.

    Article Snippet: We used the following L. monocytogenes isogenic strains: EGDe (WT, ATCC BAA-679), EGDeΔ prfA (gift from Dr M Kuhn), EGDeΔ inlA2 , EGDeΔ srtA and Δ srtB ( ).

    Techniques: Sequencing

    Vip is required for virulence. ( A ) Bacterial counts of L. monocytogenes WT, Δ vip and Δ inlA strains in the intestine, lymph nodes, liver and spleen of hEcad transgenic mice 24, 48 and 72 h after oral inoculation of 5 × 10 9 CFU. ( B ) Bacterial counts of L. monocytogenes WT and Δ vip strains in the liver, spleen and brain of mice 24, 48 and 72 h after intravenous inoculation of 10 4 CFU. ( C ) Bacterial counts of L. monocytogenes WT, Δ vip and Δ inlA strains in the intestine, lymph nodes, liver and spleen of mice 24, 48 and 72 h after oral inoculation of 5 × 10 9 CFU. ( D ) Bacterial counts of L. monocytogenes WT, Δ vip , Δ inlA or Δ vip/inlA strains in the intestine, lymph nodes, liver and spleen of guinea-pigs 72 h after oral inoculation of 10 10 CFU.

    Journal: The EMBO Journal

    Article Title: Gp96 is a receptor for a novel Listeria monocytogenes virulence factor, Vip, a surface protein

    doi: 10.1038/sj.emboj.7600750

    Figure Lengend Snippet: Vip is required for virulence. ( A ) Bacterial counts of L. monocytogenes WT, Δ vip and Δ inlA strains in the intestine, lymph nodes, liver and spleen of hEcad transgenic mice 24, 48 and 72 h after oral inoculation of 5 × 10 9 CFU. ( B ) Bacterial counts of L. monocytogenes WT and Δ vip strains in the liver, spleen and brain of mice 24, 48 and 72 h after intravenous inoculation of 10 4 CFU. ( C ) Bacterial counts of L. monocytogenes WT, Δ vip and Δ inlA strains in the intestine, lymph nodes, liver and spleen of mice 24, 48 and 72 h after oral inoculation of 5 × 10 9 CFU. ( D ) Bacterial counts of L. monocytogenes WT, Δ vip , Δ inlA or Δ vip/inlA strains in the intestine, lymph nodes, liver and spleen of guinea-pigs 72 h after oral inoculation of 10 10 CFU.

    Article Snippet: We used the following L. monocytogenes isogenic strains: EGDe (WT, ATCC BAA-679), EGDeΔ prfA (gift from Dr M Kuhn), EGDeΔ inlA2 , EGDeΔ srtA and Δ srtB ( ).

    Techniques: Transgenic Assay, Mouse Assay

    Inhibition of growth of L. monocytogenes WSLC 1364 by L. plantarum ALC 01. Ripening experiments were performed on soft cheese using a commercial, undefined multispecies microbial consortium. (A) Listeria cell counts on the cheese surface after contamination at day 1 with 2 × 10 2 CFU (open symbols) and 4 × 10 3 CFU (solid symbols) per ml of brine solution. Control cheeses were ripened with the pediocin AcH-negative type strain L. plantarum ATCC 14917. (B) Contamination with 10 2 CFU (open symbols) or 10 3 CFU (solid symbols) per ml of brine solution. Control cheeses were contaminated with the resistant mutant WSLC 1364R (pedr). (C) Listeria contamination with 7 × 10 2 CFU/ml of brine solution. In this case, cheese ripening was performed with a commercial, defined ripening culture. Either the supernatant or the pellet of the 14-h culture in VisStart TW ALC01 medium was used. Control cheeses were ripened with the addition of a 14-h culture of the pediocin AcH-negative type strain L. plantarum ATCC 14917, cultivated in VisStart TW ALC01.

    Journal: Applied and Environmental Microbiology

    Article Title: A Pediocin-Producing Lactobacillus plantarum Strain Inhibits Listeria monocytogenes in a Multispecies Cheese Surface Microbial Ripening Consortium

    doi: 10.1128/AEM.69.3.1854-1857.2003

    Figure Lengend Snippet: Inhibition of growth of L. monocytogenes WSLC 1364 by L. plantarum ALC 01. Ripening experiments were performed on soft cheese using a commercial, undefined multispecies microbial consortium. (A) Listeria cell counts on the cheese surface after contamination at day 1 with 2 × 10 2 CFU (open symbols) and 4 × 10 3 CFU (solid symbols) per ml of brine solution. Control cheeses were ripened with the pediocin AcH-negative type strain L. plantarum ATCC 14917. (B) Contamination with 10 2 CFU (open symbols) or 10 3 CFU (solid symbols) per ml of brine solution. Control cheeses were contaminated with the resistant mutant WSLC 1364R (pedr). (C) Listeria contamination with 7 × 10 2 CFU/ml of brine solution. In this case, cheese ripening was performed with a commercial, defined ripening culture. Either the supernatant or the pellet of the 14-h culture in VisStart TW ALC01 medium was used. Control cheeses were ripened with the addition of a 14-h culture of the pediocin AcH-negative type strain L. plantarum ATCC 14917, cultivated in VisStart TW ALC01.

    Article Snippet: ALC 01 produced clear zones of inhibition on solid media against all L. monocytogenes indicator strains (Table ), whereas the control strain, L. plantarum ATCC 14917, showed no inhibition.

    Techniques: Inhibition, Mutagenesis

    The growth of L. monocytogenes in pork Bulgogi at various storage temperature ( ● : 5℃, ■ : 15℃, ▲ : 25℃).

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Growth Modelling of Listeria monocytogenes in Korean Pork Bulgogi Stored at Isothermal Conditions

    doi: 10.5851/kosfa.2015.35.1.108

    Figure Lengend Snippet: The growth of L. monocytogenes in pork Bulgogi at various storage temperature ( ● : 5℃, ■ : 15℃, ▲ : 25℃).

    Article Snippet: Inoculation and enumeration A 100 μL of mixed strains of L. monocytogenes was inoculated onto each surface of pork Bulgogi and blended.

    Techniques:

    Electron micrographs of IFN-γ-activated THP-1 macrophages infected with nonadapted (a and b) and acid-adapted (c and d) L. monocytogenes LM2. A number of nonadapted bacterial cells show dramatic structural damage due to phagosomal digestion (a), whereas only a few cells can be observed free in the cytoplasm (b). Acid-adapted L. monocytogenes cells appear either intact and in active multiplication (arrow) in the phagosome (c) or free in the cytoplasm, with actin tails (d). Bars, 0.5 μm.

    Journal: Infection and Immunity

    Article Title: Effect of Acid Adaptation on the Fate of Listeria monocytogenes in THP-1 Human Macrophages Activated by Gamma Interferon

    doi: 10.1128/IAI.70.8.4369-4378.2002

    Figure Lengend Snippet: Electron micrographs of IFN-γ-activated THP-1 macrophages infected with nonadapted (a and b) and acid-adapted (c and d) L. monocytogenes LM2. A number of nonadapted bacterial cells show dramatic structural damage due to phagosomal digestion (a), whereas only a few cells can be observed free in the cytoplasm (b). Acid-adapted L. monocytogenes cells appear either intact and in active multiplication (arrow) in the phagosome (c) or free in the cytoplasm, with actin tails (d). Bars, 0.5 μm.

    Article Snippet: The bacterial strains used in this study are L. monocytogenes LM2, previously isolated in our laboratory from the spinal fluid of a newborn with listeriosis, and reference strains ATCC 7644 and EGD (kindly provided by P. Cossart).

    Techniques: Infection

    RNA slot blot analysis of actA , clpC , gad , hly , inlA , plcA , prfA , and sodC transcripts in nonadapted and acid-adapted L. monocytogenes LM2. Total RNAs were extracted from exponential cultures ( A 600 ≅ 0.4) of L. monocytogenes LM2 in BHI at pH 5.1 and 7.2. The amount of bound probe for each RNA slot was normalized by the amount of bound 16S rRNA probe. The percentage of transcription is relative to that for the non-acid-adapted condition.

    Journal: Infection and Immunity

    Article Title: Effect of Acid Adaptation on the Fate of Listeria monocytogenes in THP-1 Human Macrophages Activated by Gamma Interferon

    doi: 10.1128/IAI.70.8.4369-4378.2002

    Figure Lengend Snippet: RNA slot blot analysis of actA , clpC , gad , hly , inlA , plcA , prfA , and sodC transcripts in nonadapted and acid-adapted L. monocytogenes LM2. Total RNAs were extracted from exponential cultures ( A 600 ≅ 0.4) of L. monocytogenes LM2 in BHI at pH 5.1 and 7.2. The amount of bound probe for each RNA slot was normalized by the amount of bound 16S rRNA probe. The percentage of transcription is relative to that for the non-acid-adapted condition.

    Article Snippet: The bacterial strains used in this study are L. monocytogenes LM2, previously isolated in our laboratory from the spinal fluid of a newborn with listeriosis, and reference strains ATCC 7644 and EGD (kindly provided by P. Cossart).

    Techniques: Dot Blot

    ATR in wild-type L. monocytogenes LM2 and ATCC 7644. Survival rates are reported for acid (pH 5.1)-adapted (•) and nonadapted (○) strain LM2 and for acid-adapted (▪) and nonadapted (□) strain ATCC 7644. Bacterial counts were performed at different times of incubation in BHI acidified at pH 3.5. Error bars, SD for five independent experiments.

    Journal: Infection and Immunity

    Article Title: Effect of Acid Adaptation on the Fate of Listeria monocytogenes in THP-1 Human Macrophages Activated by Gamma Interferon

    doi: 10.1128/IAI.70.8.4369-4378.2002

    Figure Lengend Snippet: ATR in wild-type L. monocytogenes LM2 and ATCC 7644. Survival rates are reported for acid (pH 5.1)-adapted (•) and nonadapted (○) strain LM2 and for acid-adapted (▪) and nonadapted (□) strain ATCC 7644. Bacterial counts were performed at different times of incubation in BHI acidified at pH 3.5. Error bars, SD for five independent experiments.

    Article Snippet: The bacterial strains used in this study are L. monocytogenes LM2, previously isolated in our laboratory from the spinal fluid of a newborn with listeriosis, and reference strains ATCC 7644 and EGD (kindly provided by P. Cossart).

    Techniques: Incubation

    RI of L. monocytogenes LM2 in THP-1 macrophages. Circles, bacteria in nonactivated cells; triangles, bacteria in IFN-γ-activated cells. Solid symbols, acid-adapted bacteria; open symbols, nonadapted bacteria. Each data point is the mean intracellular L. monocytogenes RI from one experiment representative of six performed.

    Journal: Infection and Immunity

    Article Title: Effect of Acid Adaptation on the Fate of Listeria monocytogenes in THP-1 Human Macrophages Activated by Gamma Interferon

    doi: 10.1128/IAI.70.8.4369-4378.2002

    Figure Lengend Snippet: RI of L. monocytogenes LM2 in THP-1 macrophages. Circles, bacteria in nonactivated cells; triangles, bacteria in IFN-γ-activated cells. Solid symbols, acid-adapted bacteria; open symbols, nonadapted bacteria. Each data point is the mean intracellular L. monocytogenes RI from one experiment representative of six performed.

    Article Snippet: The bacterial strains used in this study are L. monocytogenes LM2, previously isolated in our laboratory from the spinal fluid of a newborn with listeriosis, and reference strains ATCC 7644 and EGD (kindly provided by P. Cossart).

    Techniques:

    Genomic overview of the three most up-regulated genes in L. monocytogenes upon heme exposure. Physical maps of the (A) hrtAB , hssRS , and (B) lmo1634 gene regions in strains EGD-e and LO28; the arrows indicate gene orientation. White arrows represent genes of interest, black arrows flanking genes and the gray arrows T-boxes. The numbers shown underneath each gene are the fold changes obtained from the RNA-seq analysis of the transcripts in EGD-e cells stressed with hemin, compared to the control condition. The size of the genes is drawn to scale.

    Journal: Frontiers in Microbiology

    Article Title: Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein

    doi: 10.3389/fmicb.2018.03090

    Figure Lengend Snippet: Genomic overview of the three most up-regulated genes in L. monocytogenes upon heme exposure. Physical maps of the (A) hrtAB , hssRS , and (B) lmo1634 gene regions in strains EGD-e and LO28; the arrows indicate gene orientation. White arrows represent genes of interest, black arrows flanking genes and the gray arrows T-boxes. The numbers shown underneath each gene are the fold changes obtained from the RNA-seq analysis of the transcripts in EGD-e cells stressed with hemin, compared to the control condition. The size of the genes is drawn to scale.

    Article Snippet: Bacterial Strains and Growth Conditions The wild-type strains used in this study were L. monocytogenes serotype 1/2a strain EGD-e (ATCC BAA-679), L. monocytogenes serotype 1/2c strain LO28 , and L. monocytogenes serotype 1/2a strain EGD ( ).

    Techniques: RNA Sequencing Assay

    RNA-seq analysis of Listeria monocytogenes EGD-e exposed to heme stress. (A) Distribution of differentially expressed up- and down-regulated genes with at least 4.0-fold change ( p

    Journal: Frontiers in Microbiology

    Article Title: Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein

    doi: 10.3389/fmicb.2018.03090

    Figure Lengend Snippet: RNA-seq analysis of Listeria monocytogenes EGD-e exposed to heme stress. (A) Distribution of differentially expressed up- and down-regulated genes with at least 4.0-fold change ( p

    Article Snippet: Bacterial Strains and Growth Conditions The wild-type strains used in this study were L. monocytogenes serotype 1/2a strain EGD-e (ATCC BAA-679), L. monocytogenes serotype 1/2c strain LO28 , and L. monocytogenes serotype 1/2a strain EGD ( ).

    Techniques: RNA Sequencing Assay

    Genomic locations of the selected virulence loci in L. monocytogenes strain EGD-e. The six loci analyzed are shown in boldface characters.

    Journal: Applied and Environmental Microbiology

    Article Title: Multi-Virulence-Locus Sequence Typing of Listeria monocytogenes

    doi: 10.1128/AEM.70.2.913-920.2004

    Figure Lengend Snippet: Genomic locations of the selected virulence loci in L. monocytogenes strain EGD-e. The six loci analyzed are shown in boldface characters.

    Article Snippet: PCR primers (Table ) were designed using Primer3 software ( ) on the basis of the known sequences of L. monocytogenes strain EGD-e (Lion Bioscience) ( ).

    Techniques:

    Application of model: comparison of simulated (Simu.) and observed CFU of L. monocytogenes strain ATCC 19115 in blue-white soft cheese when inoculated at day 13 (means and 95% confidence intervals). Applied parameter: μ opt = 0.43 h −1 .

    Journal: Applied and Environmental Microbiology

    Article Title: Modeling the Growth of Listeria monocytogenes in Soft Blue-White Cheese

    doi: 10.1128/AEM.01865-12

    Figure Lengend Snippet: Application of model: comparison of simulated (Simu.) and observed CFU of L. monocytogenes strain ATCC 19115 in blue-white soft cheese when inoculated at day 13 (means and 95% confidence intervals). Applied parameter: μ opt = 0.43 h −1 .

    Article Snippet: We measured and simulated growth of L. monocytogenes strain ATCC 19115 in the cheese when incubated at different temperatures ( ).

    Techniques:

    Validation of model performance: comparison of simulated (Simu.) and observed CFU of L. monocytogenes strain ATCC 19115 in blue-white soft cheese when inoculated in cheeses of different ages (means and 95% confidence intervals). μ opt = 0.43 h

    Journal: Applied and Environmental Microbiology

    Article Title: Modeling the Growth of Listeria monocytogenes in Soft Blue-White Cheese

    doi: 10.1128/AEM.01865-12

    Figure Lengend Snippet: Validation of model performance: comparison of simulated (Simu.) and observed CFU of L. monocytogenes strain ATCC 19115 in blue-white soft cheese when inoculated in cheeses of different ages (means and 95% confidence intervals). μ opt = 0.43 h

    Article Snippet: We measured and simulated growth of L. monocytogenes strain ATCC 19115 in the cheese when incubated at different temperatures ( ).

    Techniques:

    Validation of model performance: comparison of simulated (Simu.) and observed CFU of L. monocytogenes strain ATCC 19115 in blue-white soft cheese when inoculated at day 73 and stored at 4°C, 9°C, and 13°C (means and 95% confidence

    Journal: Applied and Environmental Microbiology

    Article Title: Modeling the Growth of Listeria monocytogenes in Soft Blue-White Cheese

    doi: 10.1128/AEM.01865-12

    Figure Lengend Snippet: Validation of model performance: comparison of simulated (Simu.) and observed CFU of L. monocytogenes strain ATCC 19115 in blue-white soft cheese when inoculated at day 73 and stored at 4°C, 9°C, and 13°C (means and 95% confidence

    Article Snippet: We measured and simulated growth of L. monocytogenes strain ATCC 19115 in the cheese when incubated at different temperatures ( ).

    Techniques:

    Lit2 is a functional lipoprotein intramolecular transacylase. (A) The linked lnt ::Spt r and chiQ ::Apr r markers were cotransduced by P1 vir into recipient strains carrying pUC19, p lnt , p Eflit2 , or p Lmlit2 . The percentage of transductants resistant to apramycin alone versus both apramycin and spectinomycin are shown ( n = 24 to 40) and compared to negative (pUC19) and positive (p lnt ). (B) Trypsinized N-terminal lipopeptides of Lpp from the lnt -null strain KA811 expressing E. faecalis lit2 were analyzed by MALDI-TOF MS. The protonated m/z 1,157.8 and sodiated m/z 1,179.8 parent ions are shown (inset), with the latter further fragmented by MS-MS. (C) This spectrum was used to assign Lpp structure from KA811 as the lyso form. Note that the m/z 1,185.8 peak in the parent spectrum is consistent with a C 16:0 , C 18:1 acyl chain combination. (D and F) Trypsinized N-terminal lipopeptides of the lipoprotein KO07_11695 from wild-type L. monocytogenes ATCC 19115 (D) and the corresponding L. monocytogenes lit2 -expressing strain KA849 (F) were analyzed by MALDI-TOF MS. The parent spectra (insets) display the protonated m/z 1,260.9 and sodiated m/z 1,282.9 parent ions. (E and G) The sodiated ions were fragmented by MS-MS (D and F), revealing production of DA-LP in KA847 (E) and the lyso-LP when L. monocytogenes lit2 was expressed (G). (D and F) The structurally diagnostic dehydroalanyl ions for the diacylglycerol-modified (triangle) and the lyso (circle) lipopeptides are indicated.

    Journal: Journal of Bacteriology

    Article Title: Copper-Induced Expression of a Transmissible Lipoprotein Intramolecular Transacylase Alters Lipoprotein Acylation and the Toll-Like Receptor 2 Response to Listeria monocytogenes

    doi: 10.1128/JB.00195-19

    Figure Lengend Snippet: Lit2 is a functional lipoprotein intramolecular transacylase. (A) The linked lnt ::Spt r and chiQ ::Apr r markers were cotransduced by P1 vir into recipient strains carrying pUC19, p lnt , p Eflit2 , or p Lmlit2 . The percentage of transductants resistant to apramycin alone versus both apramycin and spectinomycin are shown ( n = 24 to 40) and compared to negative (pUC19) and positive (p lnt ). (B) Trypsinized N-terminal lipopeptides of Lpp from the lnt -null strain KA811 expressing E. faecalis lit2 were analyzed by MALDI-TOF MS. The protonated m/z 1,157.8 and sodiated m/z 1,179.8 parent ions are shown (inset), with the latter further fragmented by MS-MS. (C) This spectrum was used to assign Lpp structure from KA811 as the lyso form. Note that the m/z 1,185.8 peak in the parent spectrum is consistent with a C 16:0 , C 18:1 acyl chain combination. (D and F) Trypsinized N-terminal lipopeptides of the lipoprotein KO07_11695 from wild-type L. monocytogenes ATCC 19115 (D) and the corresponding L. monocytogenes lit2 -expressing strain KA849 (F) were analyzed by MALDI-TOF MS. The parent spectra (insets) display the protonated m/z 1,260.9 and sodiated m/z 1,282.9 parent ions. (E and G) The sodiated ions were fragmented by MS-MS (D and F), revealing production of DA-LP in KA847 (E) and the lyso-LP when L. monocytogenes lit2 was expressed (G). (D and F) The structurally diagnostic dehydroalanyl ions for the diacylglycerol-modified (triangle) and the lyso (circle) lipopeptides are indicated.

    Article Snippet: Thus, lipoproteins in the L. monocytogenes type strain ATCC 19115 are DA-LPs, and expression of L. monocytogenes CFSAN023459 lit2 alone is sufficient for lipoprotein conversion.

    Techniques: Functional Assay, Single-particle Tracking, Expressing, Mass Spectrometry, Diagnostic Assay, Modification

    Changes in MIC values of L. monocytogenes ATCC 19115 (strain B ), MRSA (strain C ), VRE (strain D ), and P. aeruginosa ATCC 27853 (strain I ) treated with AMK (green), 7Aa (purple), 4F (blue), 4D (orange), as well as C. albicans ATCC 10231 treated with AmB (yellow), 7Aa (purple), and 4F (blue) over 15 cycles. Numbers above the bars represent the passage when either bacterial or fungal cells developed resistance.

    Journal: Bioorganic & medicinal chemistry

    Article Title: Bis(N-amidinohydrazones) and N-(amidino)-N'-aryl-bishydrazones: New classes of antibacterial/antifungal agents

    doi: 10.1016/j.bmc.2016.10.009

    Figure Lengend Snippet: Changes in MIC values of L. monocytogenes ATCC 19115 (strain B ), MRSA (strain C ), VRE (strain D ), and P. aeruginosa ATCC 27853 (strain I ) treated with AMK (green), 7Aa (purple), 4F (blue), 4D (orange), as well as C. albicans ATCC 10231 treated with AmB (yellow), 7Aa (purple), and 4F (blue) over 15 cycles. Numbers above the bars represent the passage when either bacterial or fungal cells developed resistance.

    Article Snippet: L. monocytogenes ATCC 19115 (strain B , Lmo ), MRSA (strain C ), VRE (strain D ), and P. aeruginosa ATCC 27853 (strain I , Pae ) were exposed to sub-inhibitory concentrations of compounds 4D , 4F , 7Aa , and AMK, and were sub-cultured for 15 serial passages to determine if an increase in MIC values occurred for each compound against the strains tested.

    Techniques:

    Relative transcript expression profiles of select genes from weakly-adherent (CW35) and strongly-adherent (99-38) strains of L. monocytogenes . Panel A, from cells recovered from planktonic growth at 30 °C. Panel B, from cells attached to glass beads during growth at 30 °C. Panel C, from planktonic cells grown at 42 °C. Expression is relative to that of the reference gene, 16S rRNA. All data bars represent the means of triplicate replications for gene expression RT-qPCR assays. Error bars indicate the standard deviation from the mean. Expression was normalized (×10 7 factor) to eliminate negative expression levels.

    Journal: Pathogens

    Article Title: RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes

    doi: 10.3390/pathogens5040060

    Figure Lengend Snippet: Relative transcript expression profiles of select genes from weakly-adherent (CW35) and strongly-adherent (99-38) strains of L. monocytogenes . Panel A, from cells recovered from planktonic growth at 30 °C. Panel B, from cells attached to glass beads during growth at 30 °C. Panel C, from planktonic cells grown at 42 °C. Expression is relative to that of the reference gene, 16S rRNA. All data bars represent the means of triplicate replications for gene expression RT-qPCR assays. Error bars indicate the standard deviation from the mean. Expression was normalized (×10 7 factor) to eliminate negative expression levels.

    Article Snippet: Planktonic Cells Pelleted cells of various strains of L. monocytogenes in sterile Eppendorf tubes were prepared from 1 mL of overnight cultures in BHI broth at 30 °C or 42 °C, and washed 3 times by suspension with 1× PBS prior to total RNA extraction.

    Techniques: Expressing, Quantitative RT-PCR, Standard Deviation

    Comparison of attachment characteristics of L. monocytogenes CW35 (weakly-adherent) and 99-38 (strongly-adherent) in microplate wells. Enumeration of well cell cultures (left) and attached cells (right) after release by treatment with protease. All data represent the means of triplicate replications. Means with the same lowercase letters are not significantly different; means with different letters are significantly different ( P

    Journal: Pathogens

    Article Title: RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes

    doi: 10.3390/pathogens5040060

    Figure Lengend Snippet: Comparison of attachment characteristics of L. monocytogenes CW35 (weakly-adherent) and 99-38 (strongly-adherent) in microplate wells. Enumeration of well cell cultures (left) and attached cells (right) after release by treatment with protease. All data represent the means of triplicate replications. Means with the same lowercase letters are not significantly different; means with different letters are significantly different ( P

    Article Snippet: Planktonic Cells Pelleted cells of various strains of L. monocytogenes in sterile Eppendorf tubes were prepared from 1 mL of overnight cultures in BHI broth at 30 °C or 42 °C, and washed 3 times by suspension with 1× PBS prior to total RNA extraction.

    Techniques:

    PCR products from genomic DNA of L. monocytogenes EDGe (Panel A), 99-38 (Panel B), and CW35 (Panel C) for PCR nucleotide evaluation of lmo0723, lmo1068, lmo1076, and lmo2558. Different gene-specific primer pairs were used for PCR amplification and subsequent agarose gel analysis of products. PCR primer combinations were based on L. monocytogenes type strain EGDe (Panel A) and tested on 99-38 (Panel B) and CW35 (Panel C). Gene lmo0723 : Lane 1, 0723A (148bp); 2, 0723B (416bp); 3, 0723C (150bp); 4, 0723D (505bp); lmo1068 : 5, 1068A (149bp); 6, 1068B (438bp); 7, 1068C (149bp); 8, 1068D (440bp); 9, 1068E (147bp); lmo1076 : 10, 1076A (470bp); 11, 1076B (150bp); 12, 1076C (146bp); 13, 1076D (991bp); lmo2558 : 14, 2558A (458bp); 15, 2558B (148bp); 16, 2558C (149bp); 17, 2558D (1129bp); 18, 100bp DNA ladder; 19 and 20, positive controls.

    Journal: Pathogens

    Article Title: RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes

    doi: 10.3390/pathogens5040060

    Figure Lengend Snippet: PCR products from genomic DNA of L. monocytogenes EDGe (Panel A), 99-38 (Panel B), and CW35 (Panel C) for PCR nucleotide evaluation of lmo0723, lmo1068, lmo1076, and lmo2558. Different gene-specific primer pairs were used for PCR amplification and subsequent agarose gel analysis of products. PCR primer combinations were based on L. monocytogenes type strain EGDe (Panel A) and tested on 99-38 (Panel B) and CW35 (Panel C). Gene lmo0723 : Lane 1, 0723A (148bp); 2, 0723B (416bp); 3, 0723C (150bp); 4, 0723D (505bp); lmo1068 : 5, 1068A (149bp); 6, 1068B (438bp); 7, 1068C (149bp); 8, 1068D (440bp); 9, 1068E (147bp); lmo1076 : 10, 1076A (470bp); 11, 1076B (150bp); 12, 1076C (146bp); 13, 1076D (991bp); lmo2558 : 14, 2558A (458bp); 15, 2558B (148bp); 16, 2558C (149bp); 17, 2558D (1129bp); 18, 100bp DNA ladder; 19 and 20, positive controls.

    Article Snippet: Planktonic Cells Pelleted cells of various strains of L. monocytogenes in sterile Eppendorf tubes were prepared from 1 mL of overnight cultures in BHI broth at 30 °C or 42 °C, and washed 3 times by suspension with 1× PBS prior to total RNA extraction.

    Techniques: Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis

    Adherence of various strains of L. monocytogenes using the microplate fluorescence (5,6-CFDA) adherence assay. Weakly- and strongly-adherent strains are represented by black and red bars, respectively. Data bars represent the mean of triplicate replications. Means that share the same lowercase letters are not significantly different; means with different letters are significantly different ( P

    Journal: Pathogens

    Article Title: RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes

    doi: 10.3390/pathogens5040060

    Figure Lengend Snippet: Adherence of various strains of L. monocytogenes using the microplate fluorescence (5,6-CFDA) adherence assay. Weakly- and strongly-adherent strains are represented by black and red bars, respectively. Data bars represent the mean of triplicate replications. Means that share the same lowercase letters are not significantly different; means with different letters are significantly different ( P

    Article Snippet: Planktonic Cells Pelleted cells of various strains of L. monocytogenes in sterile Eppendorf tubes were prepared from 1 mL of overnight cultures in BHI broth at 30 °C or 42 °C, and washed 3 times by suspension with 1× PBS prior to total RNA extraction.

    Techniques: Fluorescence

    Effect of temperature (30 °C vs. 42 °C) on attachment of different adherence-variant strains of L. monocytogenes (strongly adherent: Jag167, 99-38, EGDe; weakly adherent: CW35, CW52) as determined by the microplate adherence assay. Uninoculated brain heart infusion (BHI) nutrient broth was tested as a control. All data represent the means of triplicate replications. Means with the same upper/lowercase letters are not significantly different; means with different upper/lower case letters are significantly different ( P

    Journal: Pathogens

    Article Title: RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes

    doi: 10.3390/pathogens5040060

    Figure Lengend Snippet: Effect of temperature (30 °C vs. 42 °C) on attachment of different adherence-variant strains of L. monocytogenes (strongly adherent: Jag167, 99-38, EGDe; weakly adherent: CW35, CW52) as determined by the microplate adherence assay. Uninoculated brain heart infusion (BHI) nutrient broth was tested as a control. All data represent the means of triplicate replications. Means with the same upper/lowercase letters are not significantly different; means with different upper/lower case letters are significantly different ( P

    Article Snippet: Planktonic Cells Pelleted cells of various strains of L. monocytogenes in sterile Eppendorf tubes were prepared from 1 mL of overnight cultures in BHI broth at 30 °C or 42 °C, and washed 3 times by suspension with 1× PBS prior to total RNA extraction.

    Techniques: Variant Assay

    CPE in Caco-2 monolayers. CPE was assessed with trypan blue, which stains only dead cells. (A) Uninfected control; (B) E. faecium LS10; (C) L. monocytogenes ATCC 43248; (D) L. monocytogenes ATCC 43249. Magnification, ×100.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Presence of a vanA-Carrying Pheromone Response Plasmid (pBRG1) in a Clinical Isolate of Enterococcus faecium

    doi: 10.1128/AAC.47.5.1571-1576.2003

    Figure Lengend Snippet: CPE in Caco-2 monolayers. CPE was assessed with trypan blue, which stains only dead cells. (A) Uninfected control; (B) E. faecium LS10; (C) L. monocytogenes ATCC 43248; (D) L. monocytogenes ATCC 43249. Magnification, ×100.

    Article Snippet: All strains, independently of their clinical source, proved to be capable of penetrating and surviving within Caco-2 cells, exhibiting intracellular counts similar to those of invasive, nonhemolytic L. monocytogenes strain ATCC 43248, which enters the cells but is unable to multiply intracellularly.

    Techniques:

    Absolute number of Listeria monocytogenes meningitis cases for the largest multi locus sequence type groups from 1985 to 2014 in The Netherlands. The absolute number of cases per listerial multi-locus sequence type causing meningitis in the Netherlands is shown for six time intervals. The number of listeria meningitis cases did not vary significantly over the time intervals. Data for the five most common sequence types (STs) is shown. Remaining STs are clustered in the category ‘other STs’. In the time interval 1985–1989, ST1 and ST2 were the dominant STs. In the following years, ST1 and ST2 cases decreased whereas ST6 cases increased. In 2010–2014, ST6 caused disease in most cases, followed by ST1 and ST2. The number of ST6 cases increased significantly over the years (Mann–Whitney U test, p

    Journal: Clinical Microbiology and Infection

    Article Title: Benzalkonium tolerance genes and outcome in Listeria monocytogenes meningitis

    doi: 10.1016/j.cmi.2016.12.008

    Figure Lengend Snippet: Absolute number of Listeria monocytogenes meningitis cases for the largest multi locus sequence type groups from 1985 to 2014 in The Netherlands. The absolute number of cases per listerial multi-locus sequence type causing meningitis in the Netherlands is shown for six time intervals. The number of listeria meningitis cases did not vary significantly over the time intervals. Data for the five most common sequence types (STs) is shown. Remaining STs are clustered in the category ‘other STs’. In the time interval 1985–1989, ST1 and ST2 were the dominant STs. In the following years, ST1 and ST2 cases decreased whereas ST6 cases increased. In 2010–2014, ST6 caused disease in most cases, followed by ST1 and ST2. The number of ST6 cases increased significantly over the years (Mann–Whitney U test, p

    Article Snippet: Bacterial whole genome sequencing DNA from L. monocytogenes strains was extracted based on manufacturer’s protocol (Promega, Madison, WI, USA).

    Techniques: Sequencing, MANN-WHITNEY

    Phylogenetic tree of Listeria monocytogenes isolates causing meningitis and ST6 isolates specifically. (a) Maximum likelihood analysis of core single-nucleotide polymorphisms from 96 isolates shows a population divided in two lineages and four monophyletic groups. These monophyletic groups correspond to the largest sequence type (ST) groups in the collection and single locus variants of these groups. ST8 is coloured blue, ST6 is cyan, ST2 is green, ST1 is yellow, and other STs are in red. The mutation rate is represented on the horizontal axis. Distances on the vertical axis are for visualization purposes only. (b) Pie chart of STs of 96 isolates in the study cohort. Four major ST groups hold 55% of isolates. Remaining isolates are grouped with one or two isolates of similar ST or as singletons. (c) Maximum likelihood phylogenetic tree of ST6, ST616 (single locus variant to ST6) and ST6 reference (accession number: NC_021829 ), shows a clonal expansion within the monophyletic group. These isolates more often cause unfavourable outcome in patients compared with other ST6 isolates and carry the phiLMST6 phage and the pLMST6 plasmid. Mortality and unfavourable outcome for patients infected with these isolates, and presence of phiLMST6 and pLMST6 in each isolate is coloured red.

    Journal: Clinical Microbiology and Infection

    Article Title: Benzalkonium tolerance genes and outcome in Listeria monocytogenes meningitis

    doi: 10.1016/j.cmi.2016.12.008

    Figure Lengend Snippet: Phylogenetic tree of Listeria monocytogenes isolates causing meningitis and ST6 isolates specifically. (a) Maximum likelihood analysis of core single-nucleotide polymorphisms from 96 isolates shows a population divided in two lineages and four monophyletic groups. These monophyletic groups correspond to the largest sequence type (ST) groups in the collection and single locus variants of these groups. ST8 is coloured blue, ST6 is cyan, ST2 is green, ST1 is yellow, and other STs are in red. The mutation rate is represented on the horizontal axis. Distances on the vertical axis are for visualization purposes only. (b) Pie chart of STs of 96 isolates in the study cohort. Four major ST groups hold 55% of isolates. Remaining isolates are grouped with one or two isolates of similar ST or as singletons. (c) Maximum likelihood phylogenetic tree of ST6, ST616 (single locus variant to ST6) and ST6 reference (accession number: NC_021829 ), shows a clonal expansion within the monophyletic group. These isolates more often cause unfavourable outcome in patients compared with other ST6 isolates and carry the phiLMST6 phage and the pLMST6 plasmid. Mortality and unfavourable outcome for patients infected with these isolates, and presence of phiLMST6 and pLMST6 in each isolate is coloured red.

    Article Snippet: Bacterial whole genome sequencing DNA from L. monocytogenes strains was extracted based on manufacturer’s protocol (Promega, Madison, WI, USA).

    Techniques: Sequencing, Mutagenesis, Variant Assay, Plasmid Preparation, Infection

    Real-time PCR amplifications of serial dilutions of L. monocytogenes F2365 DNA equivalent to 100,000 to 10 target molecules. Solid squares, 100,000 cell equivalents; solid circles, 10,000 cell equivalents; triangles, 1,000 cell equivalents; inverted triangles,

    Journal: Applied and Environmental Microbiology

    Article Title: Real-Time PCR Assay To Differentiate Listeriolysin S-Positive and -Negative Strains of Listeria monocytogenes ▿

    doi: 10.1128/AEM.01673-10

    Figure Lengend Snippet: Real-time PCR amplifications of serial dilutions of L. monocytogenes F2365 DNA equivalent to 100,000 to 10 target molecules. Solid squares, 100,000 cell equivalents; solid circles, 10,000 cell equivalents; triangles, 1,000 cell equivalents; inverted triangles,

    Article Snippet: Oligonucleotide primers and a FRET probe pair targeting the llsX gene of L. monocytogenes strain F2365 (National Center for Biotechnology Information [NCBI] reference sequence number ; gene locus lmof2365 _ 1115 ) were designed by following published guidelines ( ) and synthesized by Tib Molbiol (Berlin, Germany) (Table ).

    Techniques: Real-time Polymerase Chain Reaction

    Western blot analysis of extracellular proteins from different L. monocytogenes strains performed with a representative anti-PC-PLC MAb obtained in the present study. Lane 1, molecular weight markers; lane 2, ATCC 15313; lane 3, HCC23; lane 4, ATCC 19115; lane 5, HCC7; lane 6, CCF4; lane 7, EGD; lane 8, CCF1.

    Journal: Applied and Environmental Microbiology

    Article Title: Production of Monoclonal Antibodies to Listeria monocytogenes and Their Application To Determine the Virulence of Isolates from Channel Catfish †

    doi:

    Figure Lengend Snippet: Western blot analysis of extracellular proteins from different L. monocytogenes strains performed with a representative anti-PC-PLC MAb obtained in the present study. Lane 1, molecular weight markers; lane 2, ATCC 15313; lane 3, HCC23; lane 4, ATCC 19115; lane 5, HCC7; lane 6, CCF4; lane 7, EGD; lane 8, CCF1.

    Article Snippet: L. monocytogenes reference strains ATCC 15313 (serovar 1), ATCC 19115 (serovar 4b), and EGD (= NCTC 7973) (serovar 1/2a), two L. monocytogenes strains isolated from channel catfish fillets (CCF1 [serovar 1] and CCF4 [serovar 4]), and two L. monocytogenes strains isolated from various organs of healthy channel catfish (HCC7 [serovar 1] and HCC23 [serovar 4]) were used in this study.

    Techniques: Western Blot, Planar Chromatography, Molecular Weight

    Western blot analysis of extracellular proteins from different bacterial strains performed with anti-PC-PLC MAb PLC1. Lane 1, molecular weight markers; lane 2, L. monocytogenes ATCC 15313; lane 3, L. seeligeri ; lane 4, S. aureus ; lane 5, B. cereus ; lane 6, L. ivanovii ; lane 7, L. monocytogenes HCC7; lane 8, C. perfringens ; lane 9, R. equi . Anti-PC-PLC MAbs PLC2 and PLC3 gave identical results.

    Journal: Applied and Environmental Microbiology

    Article Title: Production of Monoclonal Antibodies to Listeria monocytogenes and Their Application To Determine the Virulence of Isolates from Channel Catfish †

    doi:

    Figure Lengend Snippet: Western blot analysis of extracellular proteins from different bacterial strains performed with anti-PC-PLC MAb PLC1. Lane 1, molecular weight markers; lane 2, L. monocytogenes ATCC 15313; lane 3, L. seeligeri ; lane 4, S. aureus ; lane 5, B. cereus ; lane 6, L. ivanovii ; lane 7, L. monocytogenes HCC7; lane 8, C. perfringens ; lane 9, R. equi . Anti-PC-PLC MAbs PLC2 and PLC3 gave identical results.

    Article Snippet: L. monocytogenes reference strains ATCC 15313 (serovar 1), ATCC 19115 (serovar 4b), and EGD (= NCTC 7973) (serovar 1/2a), two L. monocytogenes strains isolated from channel catfish fillets (CCF1 [serovar 1] and CCF4 [serovar 4]), and two L. monocytogenes strains isolated from various organs of healthy channel catfish (HCC7 [serovar 1] and HCC23 [serovar 4]) were used in this study.

    Techniques: Western Blot, Planar Chromatography, Molecular Weight

    Diminished clearance of L. monocytogenes in the distal small intestine of MyD88-deficient mice. (A) MyD88 +/+ and MyD88 −/− mice were infected with 10 10 L. monocytogenes by gavage, and CFUs in the proximal (pro) and distal part of the small intestinal wall were determined 24 h p.i.; n = 8 mice/group. (B) Segments from proximal (pro) or distal small intestine were cultured in media containing 2,000 L. monocytogenes. After 4 h, bacterial burden in the supernatant was determined. Values are representative of two individual experiments. (C) Schematic representation of the in vivo luminal killing assay. (D) Bioluminescence imaging of luminescent L. monocytogenes in ileal loops of MyD88 +/+ and MyD88 −/− mice. Luminescent L. monocytogenes (2.5 × 10 5 ) was injected into ileal loops of MyD88 +/+ and MyD88 −/− mice and imaged 30 min (top) and 2 h (bottom) after injection with an IVIS Imaging System. One representative mouse per group is shown. n = 5. (E) 1,000 L. monocytogenes were injected into ileal loops of wt, MyD88 −/− , TNF −/− /IFN-γ −/− , and Rip2 −/− mice. 2 h after injection, the isolated section of the intestine was harvested and CFUs in the luminal fluid were detected. n = 3 for MyD88 −/− ; n = 5–9 for all other groups. Bacterial numbers are expressed as the percentage of recovered L. monocytogenes . *, P ≤ 0.05, ***, P

    Journal: The Journal of Experimental Medicine

    Article Title: MyD88-mediated signals induce the bactericidal lectin RegIII? and protect mice against intestinal Listeria monocytogenes infection

    doi: 10.1084/jem.20070563

    Figure Lengend Snippet: Diminished clearance of L. monocytogenes in the distal small intestine of MyD88-deficient mice. (A) MyD88 +/+ and MyD88 −/− mice were infected with 10 10 L. monocytogenes by gavage, and CFUs in the proximal (pro) and distal part of the small intestinal wall were determined 24 h p.i.; n = 8 mice/group. (B) Segments from proximal (pro) or distal small intestine were cultured in media containing 2,000 L. monocytogenes. After 4 h, bacterial burden in the supernatant was determined. Values are representative of two individual experiments. (C) Schematic representation of the in vivo luminal killing assay. (D) Bioluminescence imaging of luminescent L. monocytogenes in ileal loops of MyD88 +/+ and MyD88 −/− mice. Luminescent L. monocytogenes (2.5 × 10 5 ) was injected into ileal loops of MyD88 +/+ and MyD88 −/− mice and imaged 30 min (top) and 2 h (bottom) after injection with an IVIS Imaging System. One representative mouse per group is shown. n = 5. (E) 1,000 L. monocytogenes were injected into ileal loops of wt, MyD88 −/− , TNF −/− /IFN-γ −/− , and Rip2 −/− mice. 2 h after injection, the isolated section of the intestine was harvested and CFUs in the luminal fluid were detected. n = 3 for MyD88 −/− ; n = 5–9 for all other groups. Bacterial numbers are expressed as the percentage of recovered L. monocytogenes . *, P ≤ 0.05, ***, P

    Article Snippet: For bioluminescence imaging, luminescent L. monocytogenes strain Xen 32 (Xenogen Corporation) was used.

    Techniques: Mouse Assay, Infection, Cell Culture, In Vivo, Imaging, Injection, Isolation

    Fluorescence scanning microscopy analysis of L . monocytogenes ATCC 19117 cells without (a) and treated with bacteriocin BacFL31 for 15 min (b), 30 min (c), and 1 h (d).

    Journal: BioMed Research International

    Article Title: Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes

    doi: 10.1155/2018/5308464

    Figure Lengend Snippet: Fluorescence scanning microscopy analysis of L . monocytogenes ATCC 19117 cells without (a) and treated with bacteriocin BacFL31 for 15 min (b), 30 min (c), and 1 h (d).

    Article Snippet: The indicator strain L. monocytogenes ATCC 19117 was grown in LB broth overnight at 37°C.

    Techniques: Fluorescence, Microscopy

    Membrane permeabilization assay of Bacteriocin Bac FL31 towards cytoplasmic membrane of L. monocytogenes ATCC 19117. Negative control without BacFL31 addition; positive control ethanol at 70%. Tetracycline did not show any permeability activity in this test.

    Journal: BioMed Research International

    Article Title: Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes

    doi: 10.1155/2018/5308464

    Figure Lengend Snippet: Membrane permeabilization assay of Bacteriocin Bac FL31 towards cytoplasmic membrane of L. monocytogenes ATCC 19117. Negative control without BacFL31 addition; positive control ethanol at 70%. Tetracycline did not show any permeability activity in this test.

    Article Snippet: The indicator strain L. monocytogenes ATCC 19117 was grown in LB broth overnight at 37°C.

    Techniques: BAC Assay, Negative Control, Positive Control, Permeability, Activity Assay

    Extracellular UV-absorbing materials from L. monocytogenes ATCC 19117 cells detected at 260 nm (a) and 280 nm (b).

    Journal: BioMed Research International

    Article Title: Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes

    doi: 10.1155/2018/5308464

    Figure Lengend Snippet: Extracellular UV-absorbing materials from L. monocytogenes ATCC 19117 cells detected at 260 nm (a) and 280 nm (b).

    Article Snippet: The indicator strain L. monocytogenes ATCC 19117 was grown in LB broth overnight at 37°C.

    Techniques:

    The effect of live and heat-killed Enterococcus faecium BGPAS1-3 on (A) adhesion and (B) invasion of Listeria monocytogenes ATCC 19111 on differentiated Caco-2 cells were analyzed in the competitive, the displacement and the exclusion assays. Three experiments were done. One-way ANOVA with the Tukey’s post hoc test was used to compare CFU of L. monocytogenes ATCC 19111 in cultures with and without BGPAS1-3. Statistical significance p

    Journal: Frontiers in Microbiology

    Article Title: The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111

    doi: 10.3389/fmicb.2019.00412

    Figure Lengend Snippet: The effect of live and heat-killed Enterococcus faecium BGPAS1-3 on (A) adhesion and (B) invasion of Listeria monocytogenes ATCC 19111 on differentiated Caco-2 cells were analyzed in the competitive, the displacement and the exclusion assays. Three experiments were done. One-way ANOVA with the Tukey’s post hoc test was used to compare CFU of L. monocytogenes ATCC 19111 in cultures with and without BGPAS1-3. Statistical significance p

    Article Snippet: Antimicrobial Activity Assay Efficacy of antimicrobial compounds produced by BGPAS1-3 was tested by the deferred antagonism method using indicator strain L. monocytogenes ATCC 19111 ( ).

    Techniques:

    Direct antilisterial effect of overnight culture (ON) BGPAS1-3, live BGPAS1-3 cells, supernatant (SN) BGPAS1-3 and heat-killed BGPAS1-3 of Enterococcus faecium BGPAS1-3 was tested. The zone of Listeria monocytogenes ATCC 19111 growth inhibition around the well was taken as the positive signal of antimicrobial compounds production/activity. The confirmation of the proteinaceous nature of antimicrobial compounds is seen as a detectable growth at the edge of the inhibitory zone where the dot of pronase E crystals was placed.

    Journal: Frontiers in Microbiology

    Article Title: The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111

    doi: 10.3389/fmicb.2019.00412

    Figure Lengend Snippet: Direct antilisterial effect of overnight culture (ON) BGPAS1-3, live BGPAS1-3 cells, supernatant (SN) BGPAS1-3 and heat-killed BGPAS1-3 of Enterococcus faecium BGPAS1-3 was tested. The zone of Listeria monocytogenes ATCC 19111 growth inhibition around the well was taken as the positive signal of antimicrobial compounds production/activity. The confirmation of the proteinaceous nature of antimicrobial compounds is seen as a detectable growth at the edge of the inhibitory zone where the dot of pronase E crystals was placed.

    Article Snippet: Antimicrobial Activity Assay Efficacy of antimicrobial compounds produced by BGPAS1-3 was tested by the deferred antagonism method using indicator strain L. monocytogenes ATCC 19111 ( ).

    Techniques: Inhibition, Activity Assay

    The effect of Listeria monocytogenes ATCC 19111, live and heat-killed Enterococcus faecium BGPAS1-3 on claudin expression by differentiated Caco-2 cells was analyzed on mRNA (qPCR, graphs) and protein (the representative images of Western blot, GAPDH was used as housekeeping protein) level in the competitive, the displacement and in the exclusion assays. Three experiments were done. One-way ANOVA with the Tukey’s post hoc test was used to compare the expression of mRNA for claudin (relative to β- actin as housekeeping gene) in untreated cultures with BGPAS1-3 treated and L. monocytogenes ATCC 19111 treated cultures, as well as in L. monocytogenes ATCC 19111 treated cultures with BGPAS1-3/ L. monocytogenes ATCC 19111 treated cultures. Statistical significance p

    Journal: Frontiers in Microbiology

    Article Title: The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111

    doi: 10.3389/fmicb.2019.00412

    Figure Lengend Snippet: The effect of Listeria monocytogenes ATCC 19111, live and heat-killed Enterococcus faecium BGPAS1-3 on claudin expression by differentiated Caco-2 cells was analyzed on mRNA (qPCR, graphs) and protein (the representative images of Western blot, GAPDH was used as housekeeping protein) level in the competitive, the displacement and in the exclusion assays. Three experiments were done. One-way ANOVA with the Tukey’s post hoc test was used to compare the expression of mRNA for claudin (relative to β- actin as housekeeping gene) in untreated cultures with BGPAS1-3 treated and L. monocytogenes ATCC 19111 treated cultures, as well as in L. monocytogenes ATCC 19111 treated cultures with BGPAS1-3/ L. monocytogenes ATCC 19111 treated cultures. Statistical significance p

    Article Snippet: Antimicrobial Activity Assay Efficacy of antimicrobial compounds produced by BGPAS1-3 was tested by the deferred antagonism method using indicator strain L. monocytogenes ATCC 19111 ( ).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    The effect of Listeria monocytogenes ATCC 19111, live and heat-killed Enterococcus faecium BGPAS1-3 on (A) IL-8 and (B) TGF -β mRNA expression by differentiated Caco-2 cells was analyzed in the competitive, the displacement and the exclusion assays. Three experiments were done. One-way ANOVA with the Tukey’s post hoc test was used to compare the expression of mRNA for IL-8 and TGF -β (relative to β- actin as housekeeping gene) in untreated cultures with BGPAS1-3 treated and L. monocytogenes ATCC 19111 treated cultures, as well as in L. monocytogenes ATCC 19111 treated cultures with BGPAS1-3/ L. monocytogenes ATCC 19111 treated cultures. Statistical significance p

    Journal: Frontiers in Microbiology

    Article Title: The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111

    doi: 10.3389/fmicb.2019.00412

    Figure Lengend Snippet: The effect of Listeria monocytogenes ATCC 19111, live and heat-killed Enterococcus faecium BGPAS1-3 on (A) IL-8 and (B) TGF -β mRNA expression by differentiated Caco-2 cells was analyzed in the competitive, the displacement and the exclusion assays. Three experiments were done. One-way ANOVA with the Tukey’s post hoc test was used to compare the expression of mRNA for IL-8 and TGF -β (relative to β- actin as housekeeping gene) in untreated cultures with BGPAS1-3 treated and L. monocytogenes ATCC 19111 treated cultures, as well as in L. monocytogenes ATCC 19111 treated cultures with BGPAS1-3/ L. monocytogenes ATCC 19111 treated cultures. Statistical significance p

    Article Snippet: Antimicrobial Activity Assay Efficacy of antimicrobial compounds produced by BGPAS1-3 was tested by the deferred antagonism method using indicator strain L. monocytogenes ATCC 19111 ( ).

    Techniques: Expressing

    The effect of Listeria monocytogenes ATCC 19111, live and heat-killed Enterococcus faecium BGPAS1-3 on (A) TLR2 , (B) TLR4 , and (C) MyD88 mRNA expression by differentiated Caco-2 cells was analyzed in: the competitive, the displacement and the exclusion assays. Three experiments were done. One-way ANOVA with the Tukey’s post hoc test was used to compare the expression of mRNA for TLR2, TLR4 , and MyD88 (relative to β- actin as housekeeping gene) in untreated cultures with BGPAS1-3 treated and L. monocytogenes ATCC 19111 treated cultures, as well as in L. monocytogenes ATCC 19111 treated cultures with BGPAS1-3/ L. monocytogenes ATCC 19111 treated cultures. Statistical significance p

    Journal: Frontiers in Microbiology

    Article Title: The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111

    doi: 10.3389/fmicb.2019.00412

    Figure Lengend Snippet: The effect of Listeria monocytogenes ATCC 19111, live and heat-killed Enterococcus faecium BGPAS1-3 on (A) TLR2 , (B) TLR4 , and (C) MyD88 mRNA expression by differentiated Caco-2 cells was analyzed in: the competitive, the displacement and the exclusion assays. Three experiments were done. One-way ANOVA with the Tukey’s post hoc test was used to compare the expression of mRNA for TLR2, TLR4 , and MyD88 (relative to β- actin as housekeeping gene) in untreated cultures with BGPAS1-3 treated and L. monocytogenes ATCC 19111 treated cultures, as well as in L. monocytogenes ATCC 19111 treated cultures with BGPAS1-3/ L. monocytogenes ATCC 19111 treated cultures. Statistical significance p

    Article Snippet: Antimicrobial Activity Assay Efficacy of antimicrobial compounds produced by BGPAS1-3 was tested by the deferred antagonism method using indicator strain L. monocytogenes ATCC 19111 ( ).

    Techniques: Expressing

    Molecular weight estimation of the Leuconostoc lactis SD501 bacteriocin. (A) Tricine SDS-PAGE gel stained with Coomassie Brilliant Blue G250 for protein detection. (B) Tricine gel overlaid with soft agar containing Listeria monocytogenes ATCC 19114: lanes 1 and 3, Precision Plus Protein™ Dual Xtra Prestained Protein Standards marker; lanes 2 and 4, purified bacteriocin from Leuc. lactis SD501.

    Journal: Korean Journal for Food Science of Animal Resources

    Article Title: Isolation and Characterization of an Anti-listerial Bacteriocin fromLeuconostoc lactis SD501

    doi: 10.5851/kosfa.2018.e33

    Figure Lengend Snippet: Molecular weight estimation of the Leuconostoc lactis SD501 bacteriocin. (A) Tricine SDS-PAGE gel stained with Coomassie Brilliant Blue G250 for protein detection. (B) Tricine gel overlaid with soft agar containing Listeria monocytogenes ATCC 19114: lanes 1 and 3, Precision Plus Protein™ Dual Xtra Prestained Protein Standards marker; lanes 2 and 4, purified bacteriocin from Leuc. lactis SD501.

    Article Snippet: In post-electrophoretic detection analysis, the inhibitory activity of that band was confirmed by overlaying the bacteriocin-containing gel with 0.75% (w/w) soft agar containing the indicator strain L. monocytogenes ATCC 19114 ( ).

    Techniques: Molecular Weight, SDS Page, Staining, Marker, Purification

    Genomic organization of the hypervariable hotspot 9 region in L. monocytogenes genomes harbouring homologues to proteins from plasmid pLMIV of L. monocytogenes FSL J1–208. Homologous proteins are shown as the same color. pLMIV and L. monocytogenes EGDe locus_tags are indicated.

    Journal: PLoS ONE

    Article Title: Genome Sequencing of Listeria monocytogenes "Quargel" Listeriosis Outbreak Strains Reveals Two Different Strains with Distinct In Vitro Virulence Potential

    doi: 10.1371/journal.pone.0089964

    Figure Lengend Snippet: Genomic organization of the hypervariable hotspot 9 region in L. monocytogenes genomes harbouring homologues to proteins from plasmid pLMIV of L. monocytogenes FSL J1–208. Homologous proteins are shown as the same color. pLMIV and L. monocytogenes EGDe locus_tags are indicated.

    Article Snippet: The L. monocytogenes type strain EGDe (ATCC BAA-679) was used for comparison in cell culture virulence assays.

    Techniques: Plasmid Preparation

    Invasion efficiency and intracellular proliferation of L. monocytogenes strains. Invasion efficiency (panel A and B) and intracellular growth coefficient (IGC, panel C) of type strain EGDe, QOC1 and QOC2 using four different human cell lines (intestinal epithelial Caco2, hepatocytic HepG2 and macrophage-like U937 and THP1 cells) and primary mouse bone-marrow derived macrophages (mBMDM). Values represent mean values ± SD of four biological replicates performed in duplicate. Different letters indicate statistically significant differences (P

    Journal: PLoS ONE

    Article Title: Genome Sequencing of Listeria monocytogenes "Quargel" Listeriosis Outbreak Strains Reveals Two Different Strains with Distinct In Vitro Virulence Potential

    doi: 10.1371/journal.pone.0089964

    Figure Lengend Snippet: Invasion efficiency and intracellular proliferation of L. monocytogenes strains. Invasion efficiency (panel A and B) and intracellular growth coefficient (IGC, panel C) of type strain EGDe, QOC1 and QOC2 using four different human cell lines (intestinal epithelial Caco2, hepatocytic HepG2 and macrophage-like U937 and THP1 cells) and primary mouse bone-marrow derived macrophages (mBMDM). Values represent mean values ± SD of four biological replicates performed in duplicate. Different letters indicate statistically significant differences (P

    Article Snippet: The L. monocytogenes type strain EGDe (ATCC BAA-679) was used for comparison in cell culture virulence assays.

    Techniques: Derivative Assay

    Growth, survival, intracellular multiplication, and microscopic visualization of L. monocytogenes wild-type and mutant strains. (A) L. monocytogenes EGD-e (wild type) and the GshF466 insertion mutant were cultured at 37°C in tryptic soy broth

    Journal: Journal of Bacteriology

    Article Title: A Multidomain Fusion Protein in Listeria monocytogenes Catalyzes the Two Primary Activities for Glutathione Biosynthesis

    doi: 10.1128/JB.187.11.3839-3847.2005

    Figure Lengend Snippet: Growth, survival, intracellular multiplication, and microscopic visualization of L. monocytogenes wild-type and mutant strains. (A) L. monocytogenes EGD-e (wild type) and the GshF466 insertion mutant were cultured at 37°C in tryptic soy broth

    Article Snippet: L. monocytogenes wild-type strain EGD-e (= ATCC BAA-679), the isogenic mutant GshF466, and a revertant of GshF466 were cultured at 37°C in brain heart infusion medium (BHI) (Gibco) for routine growth studies and in tryptic soy broth (TSB) (Sigma Chemicals) supplemented with 25 mM glucose for thiol analyses.

    Techniques: Mutagenesis, Cell Culture

    Comparison of consensus sequences calculated from alignments of Illumina MiSeq short-read data to a non-identical reference with four reference-guided assemblers. Genomic DNA from the Listeriosis Reference Service for Canada's (LRS) Listeria monocytogenes strain 08-5578 subculture was sequenced and indexed twelve times. The resulting reads were aligned with four reference-guided assemblers (BWA, MOSAIK, Novoalign, and SMALT) using an L. monocytogenes strain EGD-e chromosome sequence obtained from the National Center for Biotechnology Information (NCBI) archive as a reference. The NCBI strain EGD-e chromosome sequence differs from the LRS strain 08-5578 chromosome sequence at 25,347 nucleotide positions. The numbers of false positive sites (a), true positive sites (b), ambiguous sites (c), and gaps (d) present in the resulting consensus sequences relative to the calculated coverage of each assembly are shown. Lines were calculated with power regression and smoothed using a Catmull-Rom Spline.

    Journal: PLoS ONE

    Article Title: Choice of Reference Sequence and Assembler for Alignment of Listeria monocytogenes Short-Read Sequence Data Greatly Influences Rates of Error in SNP Analyses

    doi: 10.1371/journal.pone.0104579

    Figure Lengend Snippet: Comparison of consensus sequences calculated from alignments of Illumina MiSeq short-read data to a non-identical reference with four reference-guided assemblers. Genomic DNA from the Listeriosis Reference Service for Canada's (LRS) Listeria monocytogenes strain 08-5578 subculture was sequenced and indexed twelve times. The resulting reads were aligned with four reference-guided assemblers (BWA, MOSAIK, Novoalign, and SMALT) using an L. monocytogenes strain EGD-e chromosome sequence obtained from the National Center for Biotechnology Information (NCBI) archive as a reference. The NCBI strain EGD-e chromosome sequence differs from the LRS strain 08-5578 chromosome sequence at 25,347 nucleotide positions. The numbers of false positive sites (a), true positive sites (b), ambiguous sites (c), and gaps (d) present in the resulting consensus sequences relative to the calculated coverage of each assembly are shown. Lines were calculated with power regression and smoothed using a Catmull-Rom Spline.

    Article Snippet: An L. monocytogenes strain EGD-e chromosome sequence obtained from the National Center for Biotechnology Information archive that differs at 25,347 nucleotide positions was used as a reference.

    Techniques: Sequencing

    Comparison of consensus sequences calculated from assemblies of simulated Illumina short-read data aligned to references of different genetic distances with four reference-guided assemblers. Ten sets of simulated sequencing reads were generated using a Listeria monocytogenes strain 08-5578 chromosome sequence obtained from the National Center for Biotechnology Information archive as a reference. Nucleotide variants were randomly introduced (10 1 –10 5 ) in silico to the 08-5578 chromosome sequence to simulate the presence of SNPs in five reference sequences. The performance of four reference-guided short-read sequence assemblers (BWA, MOSAIK, Novoalign, and SMALT) was assessed by averaging the percentages of true SNPs detected (a) and the numbers of gaps present (b) in the consensus sequences generated from alignments of the ten sets of reads. In addition, average assembly processing times are provided (c).

    Journal: PLoS ONE

    Article Title: Choice of Reference Sequence and Assembler for Alignment of Listeria monocytogenes Short-Read Sequence Data Greatly Influences Rates of Error in SNP Analyses

    doi: 10.1371/journal.pone.0104579

    Figure Lengend Snippet: Comparison of consensus sequences calculated from assemblies of simulated Illumina short-read data aligned to references of different genetic distances with four reference-guided assemblers. Ten sets of simulated sequencing reads were generated using a Listeria monocytogenes strain 08-5578 chromosome sequence obtained from the National Center for Biotechnology Information archive as a reference. Nucleotide variants were randomly introduced (10 1 –10 5 ) in silico to the 08-5578 chromosome sequence to simulate the presence of SNPs in five reference sequences. The performance of four reference-guided short-read sequence assemblers (BWA, MOSAIK, Novoalign, and SMALT) was assessed by averaging the percentages of true SNPs detected (a) and the numbers of gaps present (b) in the consensus sequences generated from alignments of the ten sets of reads. In addition, average assembly processing times are provided (c).

    Article Snippet: An L. monocytogenes strain 08-5578 chromosome sequence obtained from the National Center for Biotechnology Information archive that differs at three nucleotide positions was used as a reference.

    Techniques: Sequencing, Generated, In Silico

    Comparison of consensus sequences calculated from alignments of Illumina MiSeq short-read data to a nearly identical reference with four reference-guided assemblers. Genomic DNA from the Listeriosis Reference Service for Canada's (LRS) Listeria monocytogenes strain 08-5578 subculture was indexed and sequenced twelve times. The resulting reads were aligned with four reference-guided assemblers (BWA, MOSAIK, Novoalign, and SMALT) using an L. monocytogenes strain 08-5578 chromosome sequence obtained from the National Center for Biotechnology Information (NCBI) archive as a reference. The NCBI strain 08-5578 chromosome sequence differs from the LRS strain 08-5578 chromosome sequence at three nucleotide positions. The numbers of false positive sites (a), true positive sites (b), ambiguous sites (c), and gaps (d) present in the resulting consensus sequences relative to the calculated coverage of each assembly are shown. Lines were calculated with power regression and smoothed using a Catmull-Rom Spline.

    Journal: PLoS ONE

    Article Title: Choice of Reference Sequence and Assembler for Alignment of Listeria monocytogenes Short-Read Sequence Data Greatly Influences Rates of Error in SNP Analyses

    doi: 10.1371/journal.pone.0104579

    Figure Lengend Snippet: Comparison of consensus sequences calculated from alignments of Illumina MiSeq short-read data to a nearly identical reference with four reference-guided assemblers. Genomic DNA from the Listeriosis Reference Service for Canada's (LRS) Listeria monocytogenes strain 08-5578 subculture was indexed and sequenced twelve times. The resulting reads were aligned with four reference-guided assemblers (BWA, MOSAIK, Novoalign, and SMALT) using an L. monocytogenes strain 08-5578 chromosome sequence obtained from the National Center for Biotechnology Information (NCBI) archive as a reference. The NCBI strain 08-5578 chromosome sequence differs from the LRS strain 08-5578 chromosome sequence at three nucleotide positions. The numbers of false positive sites (a), true positive sites (b), ambiguous sites (c), and gaps (d) present in the resulting consensus sequences relative to the calculated coverage of each assembly are shown. Lines were calculated with power regression and smoothed using a Catmull-Rom Spline.

    Article Snippet: An L. monocytogenes strain 08-5578 chromosome sequence obtained from the National Center for Biotechnology Information archive that differs at three nucleotide positions was used as a reference.

    Techniques: Sequencing

    Comparison of consensus sequences calculated from alignments of Illumina MiSeq short-read data to a non-identical reference with four reference-guided assemblers. Genomic DNA from the Listeriosis Reference Service for Canada's (LRS) Listeria monocytogenes strain 08-5578 subculture was sequenced and indexed twelve times. The resulting reads were aligned with four reference-guided assemblers (BWA, MOSAIK, Novoalign, and SMALT) using an L. monocytogenes strain EGD-e chromosome sequence obtained from the National Center for Biotechnology Information (NCBI) archive as a reference. The NCBI strain EGD-e chromosome sequence differs from the LRS strain 08-5578 chromosome sequence at 25,347 nucleotide positions. The numbers of false positive sites (a), true positive sites (b), ambiguous sites (c), and gaps (d) present in the resulting consensus sequences relative to the calculated coverage of each assembly are shown. Lines were calculated with power regression and smoothed using a Catmull-Rom Spline.

    Journal: PLoS ONE

    Article Title: Choice of Reference Sequence and Assembler for Alignment of Listeria monocytogenes Short-Read Sequence Data Greatly Influences Rates of Error in SNP Analyses

    doi: 10.1371/journal.pone.0104579

    Figure Lengend Snippet: Comparison of consensus sequences calculated from alignments of Illumina MiSeq short-read data to a non-identical reference with four reference-guided assemblers. Genomic DNA from the Listeriosis Reference Service for Canada's (LRS) Listeria monocytogenes strain 08-5578 subculture was sequenced and indexed twelve times. The resulting reads were aligned with four reference-guided assemblers (BWA, MOSAIK, Novoalign, and SMALT) using an L. monocytogenes strain EGD-e chromosome sequence obtained from the National Center for Biotechnology Information (NCBI) archive as a reference. The NCBI strain EGD-e chromosome sequence differs from the LRS strain 08-5578 chromosome sequence at 25,347 nucleotide positions. The numbers of false positive sites (a), true positive sites (b), ambiguous sites (c), and gaps (d) present in the resulting consensus sequences relative to the calculated coverage of each assembly are shown. Lines were calculated with power regression and smoothed using a Catmull-Rom Spline.

    Article Snippet: An L. monocytogenes strain 08-5578 chromosome sequence obtained from the National Center for Biotechnology Information archive that differs at three nucleotide positions was used as a reference.

    Techniques: Sequencing

    Rescue of the elektra phenotype by BAC transgenesis Transgenic mice were produced by microinjecting a BAC clone containing the wild-type Slfn2 sequence into single-cell embryos homozygous for the elektra mutation. (a) DNA sequence of the BAC clone and genomic DNA from an elektra homozygote in the region of the Slfn2 elektra mutation. The BAC clone contains the wild-type Slfn2 sequence. (b) Flow cytometric analysis of CD8 and CD4 staining of blood from a WT mouse, a homozygous elektra transgenic mouse carrying the Slfn2 transgene ( elektra-BAC Tg ), and a littermate lacking the transgene ( elektra ). Results are representative of 4 mice per genotype. (c) Survival curves for transgenic mice ( elektra-BAC Tg , n =7), littermates without the transgene ( elektra , n =4), and WT mice ( n =5) upon challenge with 2 × 10 5 PFU of MCMV. (d) Survival curves for transgenic mice ( elektra-BAC Tg , n =7), littermates without the transgene ( elektra , n =5), and WT mice ( n =7) upon challenge with 5 × 10 5 CFU of L. monocytogenes .

    Journal: Nature immunology

    Article Title: A Slfn2 mutation causes lymphoid and myeloid immunodeficiency due to loss of immune cell quiescence

    doi: 10.1038/ni.1847

    Figure Lengend Snippet: Rescue of the elektra phenotype by BAC transgenesis Transgenic mice were produced by microinjecting a BAC clone containing the wild-type Slfn2 sequence into single-cell embryos homozygous for the elektra mutation. (a) DNA sequence of the BAC clone and genomic DNA from an elektra homozygote in the region of the Slfn2 elektra mutation. The BAC clone contains the wild-type Slfn2 sequence. (b) Flow cytometric analysis of CD8 and CD4 staining of blood from a WT mouse, a homozygous elektra transgenic mouse carrying the Slfn2 transgene ( elektra-BAC Tg ), and a littermate lacking the transgene ( elektra ). Results are representative of 4 mice per genotype. (c) Survival curves for transgenic mice ( elektra-BAC Tg , n =7), littermates without the transgene ( elektra , n =4), and WT mice ( n =5) upon challenge with 2 × 10 5 PFU of MCMV. (d) Survival curves for transgenic mice ( elektra-BAC Tg , n =7), littermates without the transgene ( elektra , n =5), and WT mice ( n =7) upon challenge with 5 × 10 5 CFU of L. monocytogenes .

    Article Snippet: For in vivo challenge with L. monocytogenes (strain 10403S; Xenogen, Inc.), bioluminescent bacteria were prepared and injected intravenously as described previously .

    Techniques: BAC Assay, Transgenic Assay, Mouse Assay, Produced, Sequencing, Mutagenesis, Flow Cytometry, Staining

    Apoptosis of homozygous elektra monocytes in response to activation signals (a) Left, Ly6C versus CD11b staining of splenocytes from uninfected or L. monocytogenes infected WT or elektra mice ( n =5 per genotype). Percentages of inflammatory monocytes and neutrophils are indicated by R1 and R2 gated cell populations, respectively. Results are representative of 3 experiments. Right, average percentage of inflammatory monocytes 48 h post-infection from spleen, blood, and bone marrow of WT or elektra mice ( n =5 each). (b) Flow cytometric analysis of WT (CD45.1 + ) and elektra (CD45.2 + ) donor CD4 + , CD8 + , B cells (B220 + ), and inflammatory monocytes (gated on CD11b + population) in the blood of CD3-deficient mixed bone marrow chimeras ( n =3). Results are representative of two experiments. (c–e) Isolated bone marrow inflammatory monocytes from WT or elektra mice ( n =2 each) cultured for 3 days in medium (untreated) or in medium supplemented with IFN-γ and heat-killed L. monocytogenes (treated). (c) Top, MHC class II staining. Bottom, nitric oxide concentration. (d) Forward-scatter (FSC) versus side-scatter (SSC) profile. Populations of live and dead cells are indicated. (e) Annexin V staining of the gated high FSC population. Results are representative of three experiments. For all panels, *** P

    Journal: Nature immunology

    Article Title: A Slfn2 mutation causes lymphoid and myeloid immunodeficiency due to loss of immune cell quiescence

    doi: 10.1038/ni.1847

    Figure Lengend Snippet: Apoptosis of homozygous elektra monocytes in response to activation signals (a) Left, Ly6C versus CD11b staining of splenocytes from uninfected or L. monocytogenes infected WT or elektra mice ( n =5 per genotype). Percentages of inflammatory monocytes and neutrophils are indicated by R1 and R2 gated cell populations, respectively. Results are representative of 3 experiments. Right, average percentage of inflammatory monocytes 48 h post-infection from spleen, blood, and bone marrow of WT or elektra mice ( n =5 each). (b) Flow cytometric analysis of WT (CD45.1 + ) and elektra (CD45.2 + ) donor CD4 + , CD8 + , B cells (B220 + ), and inflammatory monocytes (gated on CD11b + population) in the blood of CD3-deficient mixed bone marrow chimeras ( n =3). Results are representative of two experiments. (c–e) Isolated bone marrow inflammatory monocytes from WT or elektra mice ( n =2 each) cultured for 3 days in medium (untreated) or in medium supplemented with IFN-γ and heat-killed L. monocytogenes (treated). (c) Top, MHC class II staining. Bottom, nitric oxide concentration. (d) Forward-scatter (FSC) versus side-scatter (SSC) profile. Populations of live and dead cells are indicated. (e) Annexin V staining of the gated high FSC population. Results are representative of three experiments. For all panels, *** P

    Article Snippet: For in vivo challenge with L. monocytogenes (strain 10403S; Xenogen, Inc.), bioluminescent bacteria were prepared and injected intravenously as described previously .

    Techniques: Activation Assay, Staining, Infection, Mouse Assay, Flow Cytometry, Isolation, Cell Culture, Concentration Assay

    Homozygous elektra mutants are highly susceptible to MCMV, LCMV and L. monocytogenes infections (a) Survival curves for WT ( n =8) and homozygous elektra mutants ( n =8) upon challenge with 2 × 10 5 PFU of MCMV. Results are representative of five independent experiments. (b) Survival curves upon infection with 2 × 10 5 PFU of MCMV after reciprocal bone marrow transplantation. Recipient mice were reconstituted with 5 × 10 6 bone marrow cells 1 day after 10-Gy dose of irradiation. Congenic C57BL/6.SJL ( Ptprc a Pep3 b ; Ly5.1 + ), C57BL/6J ( Ptprc b Pep3 a ; Ly5.2 + ) WT or elektra mutant mice were used as both recipients and donors as indicated in the figure. C57BL/6J into C57BL/6J, n =3; C57BL/6J into elektra , n =6; elektra into C57BL/6J, n =6; elektra into elektra , n =3. Results are representative of 2 independent experiments. (c) WT and homozygous elektra mice (3 mice in each group) were i.v. injected with either 200 or 2 × 10 6 PFU of LCMV (Armstrong strain). Viral load was measured in spleens 7 days after injection. Results are representative of two independent experiments. ** P

    Journal: Nature immunology

    Article Title: A Slfn2 mutation causes lymphoid and myeloid immunodeficiency due to loss of immune cell quiescence

    doi: 10.1038/ni.1847

    Figure Lengend Snippet: Homozygous elektra mutants are highly susceptible to MCMV, LCMV and L. monocytogenes infections (a) Survival curves for WT ( n =8) and homozygous elektra mutants ( n =8) upon challenge with 2 × 10 5 PFU of MCMV. Results are representative of five independent experiments. (b) Survival curves upon infection with 2 × 10 5 PFU of MCMV after reciprocal bone marrow transplantation. Recipient mice were reconstituted with 5 × 10 6 bone marrow cells 1 day after 10-Gy dose of irradiation. Congenic C57BL/6.SJL ( Ptprc a Pep3 b ; Ly5.1 + ), C57BL/6J ( Ptprc b Pep3 a ; Ly5.2 + ) WT or elektra mutant mice were used as both recipients and donors as indicated in the figure. C57BL/6J into C57BL/6J, n =3; C57BL/6J into elektra , n =6; elektra into C57BL/6J, n =6; elektra into elektra , n =3. Results are representative of 2 independent experiments. (c) WT and homozygous elektra mice (3 mice in each group) were i.v. injected with either 200 or 2 × 10 6 PFU of LCMV (Armstrong strain). Viral load was measured in spleens 7 days after injection. Results are representative of two independent experiments. ** P

    Article Snippet: For in vivo challenge with L. monocytogenes (strain 10403S; Xenogen, Inc.), bioluminescent bacteria were prepared and injected intravenously as described previously .

    Techniques: Infection, Transplantation Assay, Mouse Assay, Irradiation, Mutagenesis, Injection

    Septin expression, localization, and recruitment at the bacterial entry site in human non-phagocytic cells. (A) Western blots (WB) of septins in non-phagocytic cell lines. HeLa and JEG-3 cells were harvested, and cell lysates were separated by 10% SDS-PAGE before immunoblotting. Blots were probed with antibodies specific to GAPDH, SEPT2, SEPT9, SEPT11, and actin. Blots for GAPDH are shown as a loading control. (B) Septin filaments partially colocalize with actin filaments in HeLa cells. Endogenous α-tubulin, F-actin, and SEPT2, SEPT9, or SEPT11 were visualized by immunostaining with anti-α-tubulin (grey and in Merge blue), anti-F-actin (green), and anti-SEPT2, anti-SEPT9, or anti-SEPT11 antibodies (red). Representative confocal images for α-tubulin, F-actin, and SEPT11 distribution in HeLa cells are here displayed, and similar images were obtained labeling for SEPT2 or SEPT9. Scale bars indicate 10 µm. (C) Septin filaments partially colocalize with microtubules in JEG-3 cells. Endogenous α–tubulin, F-actin, and SEPT2, SEPT9, or SEPT11 were visualized for immunostaining with anti-α-tubulin (grey and in Merge blue), anti-F-actin (green), and anti-SEPT2, anti-SEPT9, or anti-SEPT11 antibodies (red). Representative confocal images for α-tubulin, F-actin, and SEPT11 distribution in JEG-3 cells are here displayed, and similar images were obtained labeling for SEPT2 or SEPT9. Scale bars indicate 10 µm. (D) Septin recruitment at the site of Listeria entry in JEG-3 cells. Cells were infected with L. monocytogenes BUG 1641 for 5, 10, or 15 minutes and then fixed for microscopy. Endogenous septin was stained with anti-SEPT2, anti-SEPT9, or anti-SEPT11 antibodies (red). Actin was stained with anti-F-actin (green), and Listeria was marked using DAPI (blue). Representative photos are here displayed, where inset images highlight the septin collar-like recruitment around Listeria to which the white arrows are pointing. Scale bars indicate 1 µm. (E, F) Septin recruitment to the site of Shigella entry in (E) JEG-3 cells or (F) HeLa cells. Cells were infected with S. flexneri M90T for 15 minutes and then fixed for microscopy. Endogenous septin was stained with anti-SEPT2, anti-SEPT9, or anti-SEPT11 antibodies (red). Actin was stained with anti-F-actin (green), and Shigella was marked using DAPI (blue). Representative photos showing SEPT11 (JEG-3 cells) and SEPT2 (HeLa cells) are here displayed, where inset images highlight the septin collar-like recruitment around Shigella to which the white arrows are pointing. In JEG-3 cell and HeLa cells, similar recruitment was obtained when labeling for SEPT2, SEPT9, or SEPT11. Scale bars indicate 1 µm.

    Journal: PLoS ONE

    Article Title: Septins Regulate Bacterial Entry into Host Cells

    doi: 10.1371/journal.pone.0004196

    Figure Lengend Snippet: Septin expression, localization, and recruitment at the bacterial entry site in human non-phagocytic cells. (A) Western blots (WB) of septins in non-phagocytic cell lines. HeLa and JEG-3 cells were harvested, and cell lysates were separated by 10% SDS-PAGE before immunoblotting. Blots were probed with antibodies specific to GAPDH, SEPT2, SEPT9, SEPT11, and actin. Blots for GAPDH are shown as a loading control. (B) Septin filaments partially colocalize with actin filaments in HeLa cells. Endogenous α-tubulin, F-actin, and SEPT2, SEPT9, or SEPT11 were visualized by immunostaining with anti-α-tubulin (grey and in Merge blue), anti-F-actin (green), and anti-SEPT2, anti-SEPT9, or anti-SEPT11 antibodies (red). Representative confocal images for α-tubulin, F-actin, and SEPT11 distribution in HeLa cells are here displayed, and similar images were obtained labeling for SEPT2 or SEPT9. Scale bars indicate 10 µm. (C) Septin filaments partially colocalize with microtubules in JEG-3 cells. Endogenous α–tubulin, F-actin, and SEPT2, SEPT9, or SEPT11 were visualized for immunostaining with anti-α-tubulin (grey and in Merge blue), anti-F-actin (green), and anti-SEPT2, anti-SEPT9, or anti-SEPT11 antibodies (red). Representative confocal images for α-tubulin, F-actin, and SEPT11 distribution in JEG-3 cells are here displayed, and similar images were obtained labeling for SEPT2 or SEPT9. Scale bars indicate 10 µm. (D) Septin recruitment at the site of Listeria entry in JEG-3 cells. Cells were infected with L. monocytogenes BUG 1641 for 5, 10, or 15 minutes and then fixed for microscopy. Endogenous septin was stained with anti-SEPT2, anti-SEPT9, or anti-SEPT11 antibodies (red). Actin was stained with anti-F-actin (green), and Listeria was marked using DAPI (blue). Representative photos are here displayed, where inset images highlight the septin collar-like recruitment around Listeria to which the white arrows are pointing. Scale bars indicate 1 µm. (E, F) Septin recruitment to the site of Shigella entry in (E) JEG-3 cells or (F) HeLa cells. Cells were infected with S. flexneri M90T for 15 minutes and then fixed for microscopy. Endogenous septin was stained with anti-SEPT2, anti-SEPT9, or anti-SEPT11 antibodies (red). Actin was stained with anti-F-actin (green), and Shigella was marked using DAPI (blue). Representative photos showing SEPT11 (JEG-3 cells) and SEPT2 (HeLa cells) are here displayed, where inset images highlight the septin collar-like recruitment around Shigella to which the white arrows are pointing. In JEG-3 cell and HeLa cells, similar recruitment was obtained when labeling for SEPT2, SEPT9, or SEPT11. Scale bars indicate 1 µm.

    Article Snippet: L. monocytogenes (strain EGD BUG 600) was grown overnight at 37°C in brain heart infusion (BHI) media (Difco Laboratories), diluted 15× in fresh BHI, and cultured until OD600 nm = 0.8.

    Techniques: Expressing, Western Blot, SDS Page, Immunostaining, Labeling, Infection, Microscopy, Staining

    The impact of SEPT2-depletion in HeLa and JEG-3 cells on bacterial entry. (A, B) Western blot (WB) of (A) HeLa cells or (B) JEG-3 cells transfected with siRNA against control (CTRL) or SEPT2. Cell lysates were separated by 10% SDS-PAGE before immunoblotting. The blots were probed with antibodies specific to GAPDH, SEPT2, and actin. GAPDH is shown as a loading control. The red box outlines depleted protein levels for SEPT2. (C–F) SEPT2 regulates the invasion of L. monocytogenes and S. flexneri . Gentamicin survival assays for (C and E) L. monocytogenes EGD or (D and F) S. flexneri M90T were performed in (C and D) HeLa cells or (E and F) JEG-3 cells treated with control (CTRL) siRNA, or siRNA targeted against SEPT2. Graphs represent the relative number of intracellular bacteria found inside siRNA-treated cells after the survival assay, where CFU counts obtained from septin-depleted cells were normalized to CTRL siRNA-treated cells. On the graph CTRL siRNA is figuratively presented as 1, and data represents the mean from n ≥9 experiments. Results were analyzed for statistical significance using the Student's t-test.

    Journal: PLoS ONE

    Article Title: Septins Regulate Bacterial Entry into Host Cells

    doi: 10.1371/journal.pone.0004196

    Figure Lengend Snippet: The impact of SEPT2-depletion in HeLa and JEG-3 cells on bacterial entry. (A, B) Western blot (WB) of (A) HeLa cells or (B) JEG-3 cells transfected with siRNA against control (CTRL) or SEPT2. Cell lysates were separated by 10% SDS-PAGE before immunoblotting. The blots were probed with antibodies specific to GAPDH, SEPT2, and actin. GAPDH is shown as a loading control. The red box outlines depleted protein levels for SEPT2. (C–F) SEPT2 regulates the invasion of L. monocytogenes and S. flexneri . Gentamicin survival assays for (C and E) L. monocytogenes EGD or (D and F) S. flexneri M90T were performed in (C and D) HeLa cells or (E and F) JEG-3 cells treated with control (CTRL) siRNA, or siRNA targeted against SEPT2. Graphs represent the relative number of intracellular bacteria found inside siRNA-treated cells after the survival assay, where CFU counts obtained from septin-depleted cells were normalized to CTRL siRNA-treated cells. On the graph CTRL siRNA is figuratively presented as 1, and data represents the mean from n ≥9 experiments. Results were analyzed for statistical significance using the Student's t-test.

    Article Snippet: L. monocytogenes (strain EGD BUG 600) was grown overnight at 37°C in brain heart infusion (BHI) media (Difco Laboratories), diluted 15× in fresh BHI, and cultured until OD600 nm = 0.8.

    Techniques: Western Blot, Transfection, SDS Page, Clonogenic Cell Survival Assay