Journal: Frontiers in Microbiology
Article Title: Detection of Foodborne Pathogens Using Proteomics and Metabolomics-Based Approaches
Figure Lengend Snippet: Volcano plots of (A) L. monocytogenes , (B) S. enterica and (C) E. coli O157:H7 enriched in selective media containing the minced beef samples. Green circles (•) and red stars (★) indicate statistically significant putative biomarker metabolites found in control beef samples and spiked meat samples, respectively, after specified period of incubation (For L. monocytogenes : 24 h; for S. enterica : 18 h and E. coli O157:H7: 12 h). A few statistically significant metabolites in spiked samples are labeled in the volcano plots (see Tables 2 – 4 for top ten statistically significant metabolites). Please refer to Supplementary Information for list of other statistically significant metabolites. Blue open circles (∘) indicate metabolites that are not statistically significant for the discrimination (see Supplementary Information ). The dashed lines on the volcano plot represent a p -value of 0.05 ( Y -axis) and a fold change of 2 ( X -axis).
Article Snippet: E. coli (ATCC 8739) was used as a reference strain for external calibration of MALDI-ToF MS. Media included brain heart infusion agar (BHIA, Sigma-Aldrich, Australia), Oxoid novel enrichment (ONE) broth for L. monocytogenes (OBL, Oxoid, Hampshire, United Kingdom), ONE broth for Salmonella (OBS, Oxoid, Hampshire, United Kingdom), Rappaport-Vassiliadis Soya Peptone broth (RVSP, Sigma-Aldrich, NSW, Australia), Muller-Kauffmann tetrathionate broth supplemented with novobiocin (MKTTn, Micromedia, VIC, Australia), E. coli medium (EC, Sigma-Aldrich, NSW, Australia), EC supplement with 4-methylumbelliferyl-β-D-glucuronide (EC-Mug, Sigma-Aldrich, NSW, Australia) and tryptic soy broth supplemented with novobiocin (TSBn, Sigma-Aldrich, NSW, Australia).
Techniques: Biomarker Assay, Incubation, Labeling