l-cysteine Search Results


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  • 94
    Valiant s l methionine l cysteine
    S L Methionine L Cysteine, supplied by Valiant, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore l cysteine
    L Cysteine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore n acetyl l cysteine
    HPV16/18 E7 induces LDHA nuclear translocation by ROS accumulation. a LDHA is significantly translocated into nucleus in HPV16 positive cervical cancer tissues. Representative IHC images for LDHA localization in HPV16-negative and positive cervical tumor samples. Scale bar, 100 μm. b Nuclear LDHA is dramatically increased in HPV16-positive cervical cancer tissues. Semi-quantitative cytoplasmic LDHA and nuclear LDHA scoring was performed in HPV16 negative ( n = 27) and positive ( n = 39) cervical tumor samples. c , d HPV16/18 E7 promotes LDHA nuclear translocation in a ROS-dependent manner. Primary human cervix keratinocytes (PHKs) stably expressing vector or Flag-tagged HPV16/18 E7 were treated with or without 1 mM <t>NAC</t> for 6 h, followed by staining with anti-LDHA (green), anti-Flag (red) antibodies, and <t>DAPI</t> (blue). Scale bars, 10 μm ( c ). The percentage of cells with nucleus-localized LDHA compared to total cell number was quantified ( d ). e HPV16/18 E7 enhances ROS production. Cellular ROS were measured in PHKs stably expressing vector or HPV16/18 E7 coupled with or without 1 mM NAC treatment for 6 h, followed by using the ROS-sensitive fluorescent dye (CM-H 2 DCFDA) with flow cytometry according to the manufacturer’s protocol. f , g LDHA nuclear translocation is profoundly increased in an H 2 O 2 -dose-dependent manner. Immunofluorescent images of LDHA (green) in HaCaT cells upon different dose of H 2 O 2 treatment as indicated. DAPI, blue. Scale bars, 10 μm ( f ). The percentage of cells with nucleus-localized LDHA compared with total cell number was quantified ( g ). h ROS promote LDHA nuclear translocation. HT-3 cells were treated with or without 10 μM H 2 O 2 for 6 h, and supplemented with or without 1 mM NAC for extended 6 h as indicated for nuclear isolating, followed by blotting with LDHA, Tubulin, and Lamin B1. Results are representative of three independent experiments. All data are shown as mean ± SEM. The p values were determined by two-tailed t -test. The values of p
    N Acetyl L Cysteine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3475 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher cysteine
    HPV16/18 E7 induces LDHA nuclear translocation by ROS accumulation. a LDHA is significantly translocated into nucleus in HPV16 positive cervical cancer tissues. Representative IHC images for LDHA localization in HPV16-negative and positive cervical tumor samples. Scale bar, 100 μm. b Nuclear LDHA is dramatically increased in HPV16-positive cervical cancer tissues. Semi-quantitative cytoplasmic LDHA and nuclear LDHA scoring was performed in HPV16 negative ( n = 27) and positive ( n = 39) cervical tumor samples. c , d HPV16/18 E7 promotes LDHA nuclear translocation in a ROS-dependent manner. Primary human cervix keratinocytes (PHKs) stably expressing vector or Flag-tagged HPV16/18 E7 were treated with or without 1 mM <t>NAC</t> for 6 h, followed by staining with anti-LDHA (green), anti-Flag (red) antibodies, and <t>DAPI</t> (blue). Scale bars, 10 μm ( c ). The percentage of cells with nucleus-localized LDHA compared to total cell number was quantified ( d ). e HPV16/18 E7 enhances ROS production. Cellular ROS were measured in PHKs stably expressing vector or HPV16/18 E7 coupled with or without 1 mM NAC treatment for 6 h, followed by using the ROS-sensitive fluorescent dye (CM-H 2 DCFDA) with flow cytometry according to the manufacturer’s protocol. f , g LDHA nuclear translocation is profoundly increased in an H 2 O 2 -dose-dependent manner. Immunofluorescent images of LDHA (green) in HaCaT cells upon different dose of H 2 O 2 treatment as indicated. DAPI, blue. Scale bars, 10 μm ( f ). The percentage of cells with nucleus-localized LDHA compared with total cell number was quantified ( g ). h ROS promote LDHA nuclear translocation. HT-3 cells were treated with or without 10 μM H 2 O 2 for 6 h, and supplemented with or without 1 mM NAC for extended 6 h as indicated for nuclear isolating, followed by blotting with LDHA, Tubulin, and Lamin B1. Results are representative of three independent experiments. All data are shown as mean ± SEM. The p values were determined by two-tailed t -test. The values of p
    Cysteine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore l cysteine hydrochloride
    HPV16/18 E7 induces LDHA nuclear translocation by ROS accumulation. a LDHA is significantly translocated into nucleus in HPV16 positive cervical cancer tissues. Representative IHC images for LDHA localization in HPV16-negative and positive cervical tumor samples. Scale bar, 100 μm. b Nuclear LDHA is dramatically increased in HPV16-positive cervical cancer tissues. Semi-quantitative cytoplasmic LDHA and nuclear LDHA scoring was performed in HPV16 negative ( n = 27) and positive ( n = 39) cervical tumor samples. c , d HPV16/18 E7 promotes LDHA nuclear translocation in a ROS-dependent manner. Primary human cervix keratinocytes (PHKs) stably expressing vector or Flag-tagged HPV16/18 E7 were treated with or without 1 mM <t>NAC</t> for 6 h, followed by staining with anti-LDHA (green), anti-Flag (red) antibodies, and <t>DAPI</t> (blue). Scale bars, 10 μm ( c ). The percentage of cells with nucleus-localized LDHA compared to total cell number was quantified ( d ). e HPV16/18 E7 enhances ROS production. Cellular ROS were measured in PHKs stably expressing vector or HPV16/18 E7 coupled with or without 1 mM NAC treatment for 6 h, followed by using the ROS-sensitive fluorescent dye (CM-H 2 DCFDA) with flow cytometry according to the manufacturer’s protocol. f , g LDHA nuclear translocation is profoundly increased in an H 2 O 2 -dose-dependent manner. Immunofluorescent images of LDHA (green) in HaCaT cells upon different dose of H 2 O 2 treatment as indicated. DAPI, blue. Scale bars, 10 μm ( f ). The percentage of cells with nucleus-localized LDHA compared with total cell number was quantified ( g ). h ROS promote LDHA nuclear translocation. HT-3 cells were treated with or without 10 μM H 2 O 2 for 6 h, and supplemented with or without 1 mM NAC for extended 6 h as indicated for nuclear isolating, followed by blotting with LDHA, Tubulin, and Lamin B1. Results are representative of three independent experiments. All data are shown as mean ± SEM. The p values were determined by two-tailed t -test. The values of p
    L Cysteine Hydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cysteine free dmem
    HPV16/18 E7 induces LDHA nuclear translocation by ROS accumulation. a LDHA is significantly translocated into nucleus in HPV16 positive cervical cancer tissues. Representative IHC images for LDHA localization in HPV16-negative and positive cervical tumor samples. Scale bar, 100 μm. b Nuclear LDHA is dramatically increased in HPV16-positive cervical cancer tissues. Semi-quantitative cytoplasmic LDHA and nuclear LDHA scoring was performed in HPV16 negative ( n = 27) and positive ( n = 39) cervical tumor samples. c , d HPV16/18 E7 promotes LDHA nuclear translocation in a ROS-dependent manner. Primary human cervix keratinocytes (PHKs) stably expressing vector or Flag-tagged HPV16/18 E7 were treated with or without 1 mM <t>NAC</t> for 6 h, followed by staining with anti-LDHA (green), anti-Flag (red) antibodies, and <t>DAPI</t> (blue). Scale bars, 10 μm ( c ). The percentage of cells with nucleus-localized LDHA compared to total cell number was quantified ( d ). e HPV16/18 E7 enhances ROS production. Cellular ROS were measured in PHKs stably expressing vector or HPV16/18 E7 coupled with or without 1 mM NAC treatment for 6 h, followed by using the ROS-sensitive fluorescent dye (CM-H 2 DCFDA) with flow cytometry according to the manufacturer’s protocol. f , g LDHA nuclear translocation is profoundly increased in an H 2 O 2 -dose-dependent manner. Immunofluorescent images of LDHA (green) in HaCaT cells upon different dose of H 2 O 2 treatment as indicated. DAPI, blue. Scale bars, 10 μm ( f ). The percentage of cells with nucleus-localized LDHA compared with total cell number was quantified ( g ). h ROS promote LDHA nuclear translocation. HT-3 cells were treated with or without 10 μM H 2 O 2 for 6 h, and supplemented with or without 1 mM NAC for extended 6 h as indicated for nuclear isolating, followed by blotting with LDHA, Tubulin, and Lamin B1. Results are representative of three independent experiments. All data are shown as mean ± SEM. The p values were determined by two-tailed t -test. The values of p
    Cysteine Free Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Beyotime n acetyl l cysteine nac
    Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( <t>NAC</t> ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P
    N Acetyl L Cysteine Nac, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Millipore l cysteine hydrochloride monohydrate
    Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( <t>NAC</t> ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P
    L Cysteine Hydrochloride Monohydrate, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore s trityl l cysteine
    Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( <t>NAC</t> ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P
    S Trityl L Cysteine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck & Co cysteine
    Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( <t>NAC</t> ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P
    Cysteine, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore dl cysteine
    Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( <t>NAC</t> ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P
    Dl Cysteine, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore methionine cysteine free dmem
    Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( <t>NAC</t> ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P
    Methionine Cysteine Free Dmem, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    DuPont de Nemours s methionine cysteine
    Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( <t>NAC</t> ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P
    S Methionine Cysteine, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 92/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Becton Dickinson cysteine heart agar
    Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( <t>NAC</t> ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P
    Cysteine Heart Agar, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA l cysteine
    Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( <t>NAC</t> ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P
    L Cysteine, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Syntaxin cysteine string protein
    Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( <t>NAC</t> ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P
    Cysteine String Protein, supplied by Syntaxin, used in various techniques. Bioz Stars score: 88/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Difco cysteine heart agar
    Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( <t>NAC</t> ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P
    Cysteine Heart Agar, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    HPV16/18 E7 induces LDHA nuclear translocation by ROS accumulation. a LDHA is significantly translocated into nucleus in HPV16 positive cervical cancer tissues. Representative IHC images for LDHA localization in HPV16-negative and positive cervical tumor samples. Scale bar, 100 μm. b Nuclear LDHA is dramatically increased in HPV16-positive cervical cancer tissues. Semi-quantitative cytoplasmic LDHA and nuclear LDHA scoring was performed in HPV16 negative ( n = 27) and positive ( n = 39) cervical tumor samples. c , d HPV16/18 E7 promotes LDHA nuclear translocation in a ROS-dependent manner. Primary human cervix keratinocytes (PHKs) stably expressing vector or Flag-tagged HPV16/18 E7 were treated with or without 1 mM NAC for 6 h, followed by staining with anti-LDHA (green), anti-Flag (red) antibodies, and DAPI (blue). Scale bars, 10 μm ( c ). The percentage of cells with nucleus-localized LDHA compared to total cell number was quantified ( d ). e HPV16/18 E7 enhances ROS production. Cellular ROS were measured in PHKs stably expressing vector or HPV16/18 E7 coupled with or without 1 mM NAC treatment for 6 h, followed by using the ROS-sensitive fluorescent dye (CM-H 2 DCFDA) with flow cytometry according to the manufacturer’s protocol. f , g LDHA nuclear translocation is profoundly increased in an H 2 O 2 -dose-dependent manner. Immunofluorescent images of LDHA (green) in HaCaT cells upon different dose of H 2 O 2 treatment as indicated. DAPI, blue. Scale bars, 10 μm ( f ). The percentage of cells with nucleus-localized LDHA compared with total cell number was quantified ( g ). h ROS promote LDHA nuclear translocation. HT-3 cells were treated with or without 10 μM H 2 O 2 for 6 h, and supplemented with or without 1 mM NAC for extended 6 h as indicated for nuclear isolating, followed by blotting with LDHA, Tubulin, and Lamin B1. Results are representative of three independent experiments. All data are shown as mean ± SEM. The p values were determined by two-tailed t -test. The values of p

    Journal: Nature Communications

    Article Title: Nuclear lactate dehydrogenase A senses ROS to produce α-hydroxybutyrate for HPV-induced cervical tumor growth

    doi: 10.1038/s41467-018-06841-7

    Figure Lengend Snippet: HPV16/18 E7 induces LDHA nuclear translocation by ROS accumulation. a LDHA is significantly translocated into nucleus in HPV16 positive cervical cancer tissues. Representative IHC images for LDHA localization in HPV16-negative and positive cervical tumor samples. Scale bar, 100 μm. b Nuclear LDHA is dramatically increased in HPV16-positive cervical cancer tissues. Semi-quantitative cytoplasmic LDHA and nuclear LDHA scoring was performed in HPV16 negative ( n = 27) and positive ( n = 39) cervical tumor samples. c , d HPV16/18 E7 promotes LDHA nuclear translocation in a ROS-dependent manner. Primary human cervix keratinocytes (PHKs) stably expressing vector or Flag-tagged HPV16/18 E7 were treated with or without 1 mM NAC for 6 h, followed by staining with anti-LDHA (green), anti-Flag (red) antibodies, and DAPI (blue). Scale bars, 10 μm ( c ). The percentage of cells with nucleus-localized LDHA compared to total cell number was quantified ( d ). e HPV16/18 E7 enhances ROS production. Cellular ROS were measured in PHKs stably expressing vector or HPV16/18 E7 coupled with or without 1 mM NAC treatment for 6 h, followed by using the ROS-sensitive fluorescent dye (CM-H 2 DCFDA) with flow cytometry according to the manufacturer’s protocol. f , g LDHA nuclear translocation is profoundly increased in an H 2 O 2 -dose-dependent manner. Immunofluorescent images of LDHA (green) in HaCaT cells upon different dose of H 2 O 2 treatment as indicated. DAPI, blue. Scale bars, 10 μm ( f ). The percentage of cells with nucleus-localized LDHA compared with total cell number was quantified ( g ). h ROS promote LDHA nuclear translocation. HT-3 cells were treated with or without 10 μM H 2 O 2 for 6 h, and supplemented with or without 1 mM NAC for extended 6 h as indicated for nuclear isolating, followed by blotting with LDHA, Tubulin, and Lamin B1. Results are representative of three independent experiments. All data are shown as mean ± SEM. The p values were determined by two-tailed t -test. The values of p

    Article Snippet: Hydrogen peroxide solution (H2 O2 ) (Sigma, 323381), N-acetyl cysteine (NAC) (Sigma, A7250), DAPI (Sigma, D9542), sodium pyruvate (Sigma, P5280), sodium (L) lactate solution (Sangon Biotech, A604046), sodium 2-ketobutyrate (Sigma, K0875), sodium 2-hydroxybutyrate (Santa Cruz, sc-258161), NADH (Sigma, N4505), EPZ004777 (Selleck, S7353), DPIC (Selleck, S8639), 50% glutaraldehyde solution (Sangon Biotech, A600875), and ML385 (MCE, HY-100523) were commercially obtained.

    Techniques: Translocation Assay, Immunohistochemistry, Stable Transfection, Expressing, Plasmid Preparation, Staining, Flow Cytometry, Cytometry, Two Tailed Test

    Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( NAC ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P

    Journal: Cancer Medicine

    Article Title: Iron overloaded polarizes macrophage to proinflammation phenotype through ROS/acetyl‐p53 pathway, et al. Iron overloaded polarizes macrophage to proinflammation phenotype through ROS/acetyl‐p53 pathway

    doi: 10.1002/cam4.1670

    Figure Lengend Snippet: Reactive oxygen species–promoted M1 polarization through inducing acetylation of p53. A, B, RAW cells were treated with iron (2.5 mg/ mL ) for 0, 2, 4, 6, and 8 h. C, D, Macrophages were pretreated for 1 h c646 (25 mmol/L) or N ‐acetyl‐ l ‐cysteine ( NAC ) (8 mmol/L), followed by 8‐h iron treatment (2.5 mg/ mL ). E, F, Macrophages were divided into five groups: control group, DMSO (0.3%) group, NAC + iron group ( NAC group), C646 + iron group (C646 group), and iron group. And control group was pretreated with PBS , DMSO group was pretreated with DMSO (0.3%), NAC group was pretreated with NAC (8 mmol/L), and C646 group was pretreated with p300/ CBP inhibitor (25 mmol/L) for 1 h. Then all group received an 8‐h iron treatment (2.5 mg/ mL ). A, B, Western blotting showed that p53 acetylation increased in a time‐dependent manner ( P

    Article Snippet: N ‐acetyl‐l ‐cysteine (NAC), dihydroethidium (DHE), and a ROS Detection Kit were purchased from Beyotime Company (Jiangsu, China).

    Techniques: Western Blot

    Iron‐polarized macrophages to M1 phenotype by inducing intracellular reactive oxygen species ( ROS ) production. A, B, DCFH ‐ DA probe was used to detected ROS with fluorescent microscopy and microplate reader. The intensity of green fluorescence and the values were correlated with ROS level (magnification ×400). And N ‐acetyl‐ l ‐cysteine ( NAC ) could effectively inhibit the production of ROS which induced by iron. C‐E, Results of Western blotting, qRT ‐ PCR and FCM showed NAC inhibited the expression of CD 86, IL ‐1β, TNF ‐α and iNOS ( P

    Journal: Cancer Medicine

    Article Title: Iron overloaded polarizes macrophage to proinflammation phenotype through ROS/acetyl‐p53 pathway, et al. Iron overloaded polarizes macrophage to proinflammation phenotype through ROS/acetyl‐p53 pathway

    doi: 10.1002/cam4.1670

    Figure Lengend Snippet: Iron‐polarized macrophages to M1 phenotype by inducing intracellular reactive oxygen species ( ROS ) production. A, B, DCFH ‐ DA probe was used to detected ROS with fluorescent microscopy and microplate reader. The intensity of green fluorescence and the values were correlated with ROS level (magnification ×400). And N ‐acetyl‐ l ‐cysteine ( NAC ) could effectively inhibit the production of ROS which induced by iron. C‐E, Results of Western blotting, qRT ‐ PCR and FCM showed NAC inhibited the expression of CD 86, IL ‐1β, TNF ‐α and iNOS ( P

    Article Snippet: N ‐acetyl‐l ‐cysteine (NAC), dihydroethidium (DHE), and a ROS Detection Kit were purchased from Beyotime Company (Jiangsu, China).

    Techniques: Microscopy, Fluorescence, Western Blot, Quantitative RT-PCR, Expressing