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    • lpa  (Echelon Biosciences)
    92
    Echelon Biosciences lpa
    a , Quantification of PG <t>and</t> <t>BMP</t> isolated from GRN +/+ (green), GRN -/- (orange), and GRN -/- + addback (blue) HeLa cell lines reveals a ~50% reduction in BMP levels in PGRN-knockout HeLa. b , Labeling of cellular lipids by feeding 14 C-arachidonic acid-albumin complex to GRN +/+ and GRN -/- HeLa cell lines (60 minutes). Inlet highlights reduced levels of BMP in Grn -/- HeLa cells, compared to control cells. c , Quantification of PG and BMP isolated from Grn +/+ (grey), Grn +/R493X (blue), and Grn R493X/R493X (purple) mouse brains reveals a similar decrease in BMP levels upon loss of PGRN in mouse brains (~50%). d , Quantification of PG and BMP isolated from the frontal lobes of control (pink), FTD-TDP43-A(sporadic-non-GRN) (green), and FTD-TDP43-A(GRN) (blue) human brains. BMP species with mono- or di-unsaturated fatty acid moieties are not different, whereas BMP species containing two docosahexanoic acid moieties (22:6/22:6) are reduced in the frontal and occipital lobes of all FTD subjects. e , (left panel) Scheme for immunoprecipitation experiment of full-length PGRN using BMP-, <t>LPA-coated,</t> or control beads at pH 7.4 or pH 5.2 from GRN +/+ , GRN -/- , and GRN -/- + addback cell lysates. (right panel) Western blotting anaylsis of full-length progranulin binding to individual beads reveals binding of progranulin to BMP-coated beads, in particular at pH 5.2. f, Model of the role of progranulin in the degradation of gangliosides. PGRN/granulin deficiency leads to reduced BMP levels through unclear mechanisms. Reduced BMP levels contribute to impaired ganglioside degradation. Eventually, this leads to lysosomal dysfunction and downstream consequences, including neuroinflammation and neurodegeneration. Oneway ANOVA followed by multigroup comparison (Dunn’s) test was performed. *p<0.05, **p<0.01, or ***p<0.001.
    Lpa, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lpa/product/Echelon Biosciences
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lpa - by Bioz Stars, 2023-03
    92/100 stars
    		  Buy from Supplier
    lipid analogs  (Echelon Biosciences)
    92
    Echelon Biosciences lipid analogs
    a , Quantification of PG <t>and</t> <t>BMP</t> isolated from GRN +/+ (green), GRN -/- (orange), and GRN -/- + addback (blue) HeLa cell lines reveals a ~50% reduction in BMP levels in PGRN-knockout HeLa. b , Labeling of cellular lipids by feeding 14 C-arachidonic acid-albumin complex to GRN +/+ and GRN -/- HeLa cell lines (60 minutes). Inlet highlights reduced levels of BMP in Grn -/- HeLa cells, compared to control cells. c , Quantification of PG and BMP isolated from Grn +/+ (grey), Grn +/R493X (blue), and Grn R493X/R493X (purple) mouse brains reveals a similar decrease in BMP levels upon loss of PGRN in mouse brains (~50%). d , Quantification of PG and BMP isolated from the frontal lobes of control (pink), FTD-TDP43-A(sporadic-non-GRN) (green), and FTD-TDP43-A(GRN) (blue) human brains. BMP species with mono- or di-unsaturated fatty acid moieties are not different, whereas BMP species containing two docosahexanoic acid moieties (22:6/22:6) are reduced in the frontal and occipital lobes of all FTD subjects. e , (left panel) Scheme for immunoprecipitation experiment of full-length PGRN using BMP-, <t>LPA-coated,</t> or control beads at pH 7.4 or pH 5.2 from GRN +/+ , GRN -/- , and GRN -/- + addback cell lysates. (right panel) Western blotting anaylsis of full-length progranulin binding to individual beads reveals binding of progranulin to BMP-coated beads, in particular at pH 5.2. f, Model of the role of progranulin in the degradation of gangliosides. PGRN/granulin deficiency leads to reduced BMP levels through unclear mechanisms. Reduced BMP levels contribute to impaired ganglioside degradation. Eventually, this leads to lysosomal dysfunction and downstream consequences, including neuroinflammation and neurodegeneration. Oneway ANOVA followed by multigroup comparison (Dunn’s) test was performed. *p<0.05, **p<0.01, or ***p<0.001.
    Lipid Analogs, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid analogs/product/Echelon Biosciences
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipid analogs - by Bioz Stars, 2023-03
    92/100 stars
    		  Buy from Supplier
    lpa beads  (Echelon Biosciences)
    86
    Echelon Biosciences lpa beads
    a , Quantification of PG <t>and</t> <t>BMP</t> isolated from GRN +/+ (green), GRN -/- (orange), and GRN -/- + addback (blue) HeLa cell lines reveals a ~50% reduction in BMP levels in PGRN-knockout HeLa. b , Labeling of cellular lipids by feeding 14 C-arachidonic acid-albumin complex to GRN +/+ and GRN -/- HeLa cell lines (60 minutes). Inlet highlights reduced levels of BMP in Grn -/- HeLa cells, compared to control cells. c , Quantification of PG and BMP isolated from Grn +/+ (grey), Grn +/R493X (blue), and Grn R493X/R493X (purple) mouse brains reveals a similar decrease in BMP levels upon loss of PGRN in mouse brains (~50%). d , Quantification of PG and BMP isolated from the frontal lobes of control (pink), FTD-TDP43-A(sporadic-non-GRN) (green), and FTD-TDP43-A(GRN) (blue) human brains. BMP species with mono- or di-unsaturated fatty acid moieties are not different, whereas BMP species containing two docosahexanoic acid moieties (22:6/22:6) are reduced in the frontal and occipital lobes of all FTD subjects. e , (left panel) Scheme for immunoprecipitation experiment of full-length PGRN using BMP-, <t>LPA-coated,</t> or control beads at pH 7.4 or pH 5.2 from GRN +/+ , GRN -/- , and GRN -/- + addback cell lysates. (right panel) Western blotting anaylsis of full-length progranulin binding to individual beads reveals binding of progranulin to BMP-coated beads, in particular at pH 5.2. f, Model of the role of progranulin in the degradation of gangliosides. PGRN/granulin deficiency leads to reduced BMP levels through unclear mechanisms. Reduced BMP levels contribute to impaired ganglioside degradation. Eventually, this leads to lysosomal dysfunction and downstream consequences, including neuroinflammation and neurodegeneration. Oneway ANOVA followed by multigroup comparison (Dunn’s) test was performed. *p<0.05, **p<0.01, or ***p<0.001.
    Lpa Beads, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lpa beads/product/Echelon Biosciences
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lpa beads - by Bioz Stars, 2023-03
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    a , Quantification of PG and BMP isolated from GRN +/+ (green), GRN -/- (orange), and GRN -/- + addback (blue) HeLa cell lines reveals a ~50% reduction in BMP levels in PGRN-knockout HeLa. b , Labeling of cellular lipids by feeding 14 C-arachidonic acid-albumin complex to GRN +/+ and GRN -/- HeLa cell lines (60 minutes). Inlet highlights reduced levels of BMP in Grn -/- HeLa cells, compared to control cells. c , Quantification of PG and BMP isolated from Grn +/+ (grey), Grn +/R493X (blue), and Grn R493X/R493X (purple) mouse brains reveals a similar decrease in BMP levels upon loss of PGRN in mouse brains (~50%). d , Quantification of PG and BMP isolated from the frontal lobes of control (pink), FTD-TDP43-A(sporadic-non-GRN) (green), and FTD-TDP43-A(GRN) (blue) human brains. BMP species with mono- or di-unsaturated fatty acid moieties are not different, whereas BMP species containing two docosahexanoic acid moieties (22:6/22:6) are reduced in the frontal and occipital lobes of all FTD subjects. e , (left panel) Scheme for immunoprecipitation experiment of full-length PGRN using BMP-, LPA-coated, or control beads at pH 7.4 or pH 5.2 from GRN +/+ , GRN -/- , and GRN -/- + addback cell lysates. (right panel) Western blotting anaylsis of full-length progranulin binding to individual beads reveals binding of progranulin to BMP-coated beads, in particular at pH 5.2. f, Model of the role of progranulin in the degradation of gangliosides. PGRN/granulin deficiency leads to reduced BMP levels through unclear mechanisms. Reduced BMP levels contribute to impaired ganglioside degradation. Eventually, this leads to lysosomal dysfunction and downstream consequences, including neuroinflammation and neurodegeneration. Oneway ANOVA followed by multigroup comparison (Dunn’s) test was performed. *p<0.05, **p<0.01, or ***p<0.001.

    Journal: bioRxiv

    Article Title: Progranulin deficiency results in reduced bis(monoacylglycero)phosphate (BMP) levels and gangliosidosis

    doi: 10.1101/2021.09.30.461806

    Figure Lengend Snippet: a , Quantification of PG and BMP isolated from GRN +/+ (green), GRN -/- (orange), and GRN -/- + addback (blue) HeLa cell lines reveals a ~50% reduction in BMP levels in PGRN-knockout HeLa. b , Labeling of cellular lipids by feeding 14 C-arachidonic acid-albumin complex to GRN +/+ and GRN -/- HeLa cell lines (60 minutes). Inlet highlights reduced levels of BMP in Grn -/- HeLa cells, compared to control cells. c , Quantification of PG and BMP isolated from Grn +/+ (grey), Grn +/R493X (blue), and Grn R493X/R493X (purple) mouse brains reveals a similar decrease in BMP levels upon loss of PGRN in mouse brains (~50%). d , Quantification of PG and BMP isolated from the frontal lobes of control (pink), FTD-TDP43-A(sporadic-non-GRN) (green), and FTD-TDP43-A(GRN) (blue) human brains. BMP species with mono- or di-unsaturated fatty acid moieties are not different, whereas BMP species containing two docosahexanoic acid moieties (22:6/22:6) are reduced in the frontal and occipital lobes of all FTD subjects. e , (left panel) Scheme for immunoprecipitation experiment of full-length PGRN using BMP-, LPA-coated, or control beads at pH 7.4 or pH 5.2 from GRN +/+ , GRN -/- , and GRN -/- + addback cell lysates. (right panel) Western blotting anaylsis of full-length progranulin binding to individual beads reveals binding of progranulin to BMP-coated beads, in particular at pH 5.2. f, Model of the role of progranulin in the degradation of gangliosides. PGRN/granulin deficiency leads to reduced BMP levels through unclear mechanisms. Reduced BMP levels contribute to impaired ganglioside degradation. Eventually, this leads to lysosomal dysfunction and downstream consequences, including neuroinflammation and neurodegeneration. Oneway ANOVA followed by multigroup comparison (Dunn’s) test was performed. *p<0.05, **p<0.01, or ***p<0.001.

    Article Snippet: 1 mg of supernatant from each sample was incubated with 20 μL of BMP-beads, LPA-beads, or naked-beads (Echelon Biosciences Cat#P-BLBP-2 Cat#L-6101 Cat#P-B000) for 3 hours on a rotator.

    Techniques: Isolation, Knock-Out, Labeling, Immunoprecipitation, Western Blot, Binding Assay