l glutamine Search Results


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  • 99
    Thermo Fisher l glutamine
    L Glutamine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l glutamine/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l glutamine - by Bioz Stars, 2021-04
    99/100 stars
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    99
    Millipore l glutamine
    L Glutamine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l glutamine/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l glutamine - by Bioz Stars, 2021-04
    99/100 stars
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    94
    GE Healthcare dmem
    In vitro characterization of the recombinant viruses encoding different-length NS1 protein. (A) A549 or PK-15 cells were infected at an MOI of 0.001 TCID 50 per cell. After washed with <t>PBS,</t> fresh medium containing 0.1 µg/ml TPCK-trypsin was added. Supernatants were sampled at 12, 24, 36 and 48 h p.i. and virus titers were determined as log 10 TCID 50 /ml in MDCK cells. The statistical analysis was performed using Student's t test. (B) Virus plaque assay was performed by inoculating confluent MDCK cells in 6-well plates with appropriate dilutions of viruses. After washed with PBS, cells were overlaid with <t>DMEM</t> (without phenol red)-0.8% agarose mixture containing 1% bovine serum albumin and 1 µg/ml TPCK-trypsin. After incubated at 37°C for 4 days, a second agar overlay containing 1∶10,000 neutral red was added.
    Dmem, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem/product/GE Healthcare
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmem - by Bioz Stars, 2021-04
    94/100 stars
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    99
    GE Healthcare rpmi 1640 medium
    In vitro characterization of the recombinant viruses encoding different-length NS1 protein. (A) A549 or PK-15 cells were infected at an MOI of 0.001 TCID 50 per cell. After washed with <t>PBS,</t> fresh medium containing 0.1 µg/ml TPCK-trypsin was added. Supernatants were sampled at 12, 24, 36 and 48 h p.i. and virus titers were determined as log 10 TCID 50 /ml in MDCK cells. The statistical analysis was performed using Student's t test. (B) Virus plaque assay was performed by inoculating confluent MDCK cells in 6-well plates with appropriate dilutions of viruses. After washed with PBS, cells were overlaid with <t>DMEM</t> (without phenol red)-0.8% agarose mixture containing 1% bovine serum albumin and 1 µg/ml TPCK-trypsin. After incubated at 37°C for 4 days, a second agar overlay containing 1∶10,000 neutral red was added.
    Rpmi 1640 Medium, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640 medium/product/GE Healthcare
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rpmi 1640 medium - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    N/A
    L Glutamine is a non essential amino acid involved in many biochemical processes It is synthesized in vivo by glutamate and ammonia It serves as a substrate for glutamine synthetase
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    Image Search Results


    In vitro characterization of the recombinant viruses encoding different-length NS1 protein. (A) A549 or PK-15 cells were infected at an MOI of 0.001 TCID 50 per cell. After washed with PBS, fresh medium containing 0.1 µg/ml TPCK-trypsin was added. Supernatants were sampled at 12, 24, 36 and 48 h p.i. and virus titers were determined as log 10 TCID 50 /ml in MDCK cells. The statistical analysis was performed using Student's t test. (B) Virus plaque assay was performed by inoculating confluent MDCK cells in 6-well plates with appropriate dilutions of viruses. After washed with PBS, cells were overlaid with DMEM (without phenol red)-0.8% agarose mixture containing 1% bovine serum albumin and 1 µg/ml TPCK-trypsin. After incubated at 37°C for 4 days, a second agar overlay containing 1∶10,000 neutral red was added.

    Journal: PLoS ONE

    Article Title: Effects of the C-Terminal Truncation in NS1 Protein of the 2009 Pandemic H1N1 Influenza Virus on Host Gene Expression

    doi: 10.1371/journal.pone.0026175

    Figure Lengend Snippet: In vitro characterization of the recombinant viruses encoding different-length NS1 protein. (A) A549 or PK-15 cells were infected at an MOI of 0.001 TCID 50 per cell. After washed with PBS, fresh medium containing 0.1 µg/ml TPCK-trypsin was added. Supernatants were sampled at 12, 24, 36 and 48 h p.i. and virus titers were determined as log 10 TCID 50 /ml in MDCK cells. The statistical analysis was performed using Student's t test. (B) Virus plaque assay was performed by inoculating confluent MDCK cells in 6-well plates with appropriate dilutions of viruses. After washed with PBS, cells were overlaid with DMEM (without phenol red)-0.8% agarose mixture containing 1% bovine serum albumin and 1 µg/ml TPCK-trypsin. After incubated at 37°C for 4 days, a second agar overlay containing 1∶10,000 neutral red was added.

    Article Snippet: Virus plaque assay of the two recombinant viruses rPR8-Mex NS1/219 and rPR8-Mex NS1/230 was performed by inoculating confluent MDCK cells in 6-well plates with appropriate dilutions of viruses in DMEM for 1 h. Cells were washed twice with PBS and then overlaid with DMEM (without phenol red, HyClone)-0.8% agarose mixture containing 1% bovine serum albumin and 1 µg/ml TPCK-trypsin.

    Techniques: In Vitro, Recombinant, Infection, Plaque Assay, Incubation