l - Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher l glutamine l
    L Glutamine L, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l glutamine l/product/Thermo Fisher
    Average 99 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    l glutamine l - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore l alanyl l glutamine
    L Alanyl L Glutamine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l alanyl l glutamine/product/Millipore
    Average 99 stars, based on 88 article reviews
    Price from $9.99 to $1999.99
    l alanyl l glutamine - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Tocris l ap5
    Diagram representing distributions of the penetrations by segment of the D2 barrel for cells used for analysis in subsequent figures for d <t>-AP5</t> ( left ) and l -AP5 animals ( right ). The penetrations in each group of animals were quite evenly spread through
    L Ap5, supplied by Tocris, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l ap5/product/Tocris
    Average 99 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    l ap5 - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore l canavanine
    Analysis of base editing patterns and efficiencies in single yeast colonies selected for <t>canavanine</t> resistance. A comparison of base editing frequencies for nCDA1-BE3, cCDA1-BE3, and selected base editors with truncated CDA1 domains is shown. Yeast cells were transformed with plasmids expressing the base editor and an sgRNA targeting the Can1–5 site. The target sequence is shown with the cytidines that can potentially undergo editing in red and the PAM in blue. If C-to-T conversion occurs at position −18 or −19 or both, the Can1 gene will be inactivated and the cell becomes resistant to canavanine. Values and error bars reflect the mean and standard deviation of three biological replicates. Source data are provided as a Source Data file. See also Table 1
    L Canavanine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l canavanine/product/Millipore
    Average 99 stars, based on 164 article reviews
    Price from $9.99 to $1999.99
    l canavanine - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore l citrulline
    Analysis of base editing patterns and efficiencies in single yeast colonies selected for <t>canavanine</t> resistance. A comparison of base editing frequencies for nCDA1-BE3, cCDA1-BE3, and selected base editors with truncated CDA1 domains is shown. Yeast cells were transformed with plasmids expressing the base editor and an sgRNA targeting the Can1–5 site. The target sequence is shown with the cytidines that can potentially undergo editing in red and the PAM in blue. If C-to-T conversion occurs at position −18 or −19 or both, the Can1 gene will be inactivated and the cell becomes resistant to canavanine. Values and error bars reflect the mean and standard deviation of three biological replicates. Source data are provided as a Source Data file. See also Table 1
    L Citrulline, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l citrulline/product/Millipore
    Average 99 stars, based on 182 article reviews
    Price from $9.99 to $1999.99
    l citrulline - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    96
    Millipore l rhamnulose
    Gel retardation analysis to evaluate the inhibitory effects of <t>rhamnulose-1-phophate</t> (A) and the phosphorylated rhamnulose produced by the His-RhaB-catalyzed reaction (B) on the DNA binding of RhaR. The 32 P-labeled P rhaEW probe (0.02 pmol) and the RhaR
    L Rhamnulose, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l rhamnulose/product/Millipore
    Average 96 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    l rhamnulose - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    99
    Millipore phytohemagglutinin l
    Effects of VAEI-treated DC on T cells. Purified human CD4 + T lymphocytes were cultured in the presence of medium (NC) or stimulated with <t>phytohemagglutinin-L</t> (PC; 10 μg/ml). Unstimulated T cells were further co-cultivated with immature DC (DC, CD4 + ), DC treated with a maturation cocktail (DC Stim, CD4 + ; 500 ng/mL LPS; 50 ng/mL TNF-alpha and 10 ng/mL IL-1beta), VAEI-treated DC (DC + VAEI, CD4 + ; Iscador® Qu Spez; 0.5 μg/ml), DC incubated with ML-depleted VAEI (DC + VAEI-ML, CD4 + ; Iscador®; 0.66 μg/ml) or VAEI and anti-ML antibody (DC + VAEI + aML-Ab, CD4 + ; 2.5 μg/ml). (A) Cell proliferation analysis was done using CFSE staining and flow cytometry. (B) CD25 surface marker expression was analyzed as a second indicator for T cell activation. Data of 6 individual experiments are presented as mean ± SD in relation to untreated T cells (NC) or untreated T cells co-cultured with immature DC (DC, CD4 + ). (C) IFN-γ release by T cells was analyzed in the supernatants of the co-cultures of 6 independent experiments using a cytokine bead array assay. Asterisks indicate significant differences between the groups (*P
    Phytohemagglutinin L, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phytohemagglutinin l/product/Millipore
    Average 99 stars, based on 179 article reviews
    Price from $9.99 to $1999.99
    phytohemagglutinin l - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore viscozyme l
    Effects of VAEI-treated DC on T cells. Purified human CD4 + T lymphocytes were cultured in the presence of medium (NC) or stimulated with <t>phytohemagglutinin-L</t> (PC; 10 μg/ml). Unstimulated T cells were further co-cultivated with immature DC (DC, CD4 + ), DC treated with a maturation cocktail (DC Stim, CD4 + ; 500 ng/mL LPS; 50 ng/mL TNF-alpha and 10 ng/mL IL-1beta), VAEI-treated DC (DC + VAEI, CD4 + ; Iscador® Qu Spez; 0.5 μg/ml), DC incubated with ML-depleted VAEI (DC + VAEI-ML, CD4 + ; Iscador®; 0.66 μg/ml) or VAEI and anti-ML antibody (DC + VAEI + aML-Ab, CD4 + ; 2.5 μg/ml). (A) Cell proliferation analysis was done using CFSE staining and flow cytometry. (B) CD25 surface marker expression was analyzed as a second indicator for T cell activation. Data of 6 individual experiments are presented as mean ± SD in relation to untreated T cells (NC) or untreated T cells co-cultured with immature DC (DC, CD4 + ). (C) IFN-γ release by T cells was analyzed in the supernatants of the co-cultures of 6 independent experiments using a cytokine bead array assay. Asterisks indicate significant differences between the groups (*P
    Viscozyme L, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viscozyme l/product/Millipore
    Average 99 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    viscozyme l - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    91
    Millipore l fucitol
    Effects of VAEI-treated DC on T cells. Purified human CD4 + T lymphocytes were cultured in the presence of medium (NC) or stimulated with <t>phytohemagglutinin-L</t> (PC; 10 μg/ml). Unstimulated T cells were further co-cultivated with immature DC (DC, CD4 + ), DC treated with a maturation cocktail (DC Stim, CD4 + ; 500 ng/mL LPS; 50 ng/mL TNF-alpha and 10 ng/mL IL-1beta), VAEI-treated DC (DC + VAEI, CD4 + ; Iscador® Qu Spez; 0.5 μg/ml), DC incubated with ML-depleted VAEI (DC + VAEI-ML, CD4 + ; Iscador®; 0.66 μg/ml) or VAEI and anti-ML antibody (DC + VAEI + aML-Ab, CD4 + ; 2.5 μg/ml). (A) Cell proliferation analysis was done using CFSE staining and flow cytometry. (B) CD25 surface marker expression was analyzed as a second indicator for T cell activation. Data of 6 individual experiments are presented as mean ± SD in relation to untreated T cells (NC) or untreated T cells co-cultured with immature DC (DC, CD4 + ). (C) IFN-γ release by T cells was analyzed in the supernatants of the co-cultures of 6 independent experiments using a cytokine bead array assay. Asterisks indicate significant differences between the groups (*P
    L Fucitol, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l fucitol/product/Millipore
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    l fucitol - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    99
    Millipore l glyceraldehyde
    Effects of VAEI-treated DC on T cells. Purified human CD4 + T lymphocytes were cultured in the presence of medium (NC) or stimulated with <t>phytohemagglutinin-L</t> (PC; 10 μg/ml). Unstimulated T cells were further co-cultivated with immature DC (DC, CD4 + ), DC treated with a maturation cocktail (DC Stim, CD4 + ; 500 ng/mL LPS; 50 ng/mL TNF-alpha and 10 ng/mL IL-1beta), VAEI-treated DC (DC + VAEI, CD4 + ; Iscador® Qu Spez; 0.5 μg/ml), DC incubated with ML-depleted VAEI (DC + VAEI-ML, CD4 + ; Iscador®; 0.66 μg/ml) or VAEI and anti-ML antibody (DC + VAEI + aML-Ab, CD4 + ; 2.5 μg/ml). (A) Cell proliferation analysis was done using CFSE staining and flow cytometry. (B) CD25 surface marker expression was analyzed as a second indicator for T cell activation. Data of 6 individual experiments are presented as mean ± SD in relation to untreated T cells (NC) or untreated T cells co-cultured with immature DC (DC, CD4 + ). (C) IFN-γ release by T cells was analyzed in the supernatants of the co-cultures of 6 independent experiments using a cytokine bead array assay. Asterisks indicate significant differences between the groups (*P
    L Glyceraldehyde, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l glyceraldehyde/product/Millipore
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    l glyceraldehyde - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore l homocystine
    Effects of Hcy on CSE expression and promoter activity in raw264.7 macrophages. ( A – D ) Hcy down-regulated CSE mRNA and protein expressions in raw264.7 macrophages in dose- and time-dependent manners ( E , F ). Effect of l <t>-homocystine</t> and l -cysteine on CSE protein expression. CSE protein expression was evaluated by immunoblotting analysis while CSE mRNA level determined by quantitative PCR. GAPDH protein and 18S mRNA served as loading controls. Mean ± SEM, n = 3–8. ( G ) Reporter assay of a series of different fragments of cse promoter activity in raw264.7 cells that were treated with 100 or 500 µM Hcy for 12 h. Luciferase activities were expressed as fold increases over pGL3-basic vector. n = 7. * p
    L Homocystine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l homocystine/product/Millipore
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    l homocystine - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Tocris l nna
    Effects of Hcy on CSE expression and promoter activity in raw264.7 macrophages. ( A – D ) Hcy down-regulated CSE mRNA and protein expressions in raw264.7 macrophages in dose- and time-dependent manners ( E , F ). Effect of l <t>-homocystine</t> and l -cysteine on CSE protein expression. CSE protein expression was evaluated by immunoblotting analysis while CSE mRNA level determined by quantitative PCR. GAPDH protein and 18S mRNA served as loading controls. Mean ± SEM, n = 3–8. ( G ) Reporter assay of a series of different fragments of cse promoter activity in raw264.7 cells that were treated with 100 or 500 µM Hcy for 12 h. Luciferase activities were expressed as fold increases over pGL3-basic vector. n = 7. * p
    L Nna, supplied by Tocris, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l nna/product/Tocris
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    l nna - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore l ethionine
    Effects of Hcy on CSE expression and promoter activity in raw264.7 macrophages. ( A – D ) Hcy down-regulated CSE mRNA and protein expressions in raw264.7 macrophages in dose- and time-dependent manners ( E , F ). Effect of l <t>-homocystine</t> and l -cysteine on CSE protein expression. CSE protein expression was evaluated by immunoblotting analysis while CSE mRNA level determined by quantitative PCR. GAPDH protein and 18S mRNA served as loading controls. Mean ± SEM, n = 3–8. ( G ) Reporter assay of a series of different fragments of cse promoter activity in raw264.7 cells that were treated with 100 or 500 µM Hcy for 12 h. Luciferase activities were expressed as fold increases over pGL3-basic vector. n = 7. * p
    L Ethionine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l ethionine/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    l ethionine - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    91
    Millipore l threoninol
    Effects of Hcy on CSE expression and promoter activity in raw264.7 macrophages. ( A – D ) Hcy down-regulated CSE mRNA and protein expressions in raw264.7 macrophages in dose- and time-dependent manners ( E , F ). Effect of l <t>-homocystine</t> and l -cysteine on CSE protein expression. CSE protein expression was evaluated by immunoblotting analysis while CSE mRNA level determined by quantitative PCR. GAPDH protein and 18S mRNA served as loading controls. Mean ± SEM, n = 3–8. ( G ) Reporter assay of a series of different fragments of cse promoter activity in raw264.7 cells that were treated with 100 or 500 µM Hcy for 12 h. Luciferase activities were expressed as fold increases over pGL3-basic vector. n = 7. * p
    L Threoninol, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l threoninol/product/Millipore
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l threoninol - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    94
    Millipore l alanyl l alanine lala
    Effects of Hcy on CSE expression and promoter activity in raw264.7 macrophages. ( A – D ) Hcy down-regulated CSE mRNA and protein expressions in raw264.7 macrophages in dose- and time-dependent manners ( E , F ). Effect of l <t>-homocystine</t> and l -cysteine on CSE protein expression. CSE protein expression was evaluated by immunoblotting analysis while CSE mRNA level determined by quantitative PCR. GAPDH protein and 18S mRNA served as loading controls. Mean ± SEM, n = 3–8. ( G ) Reporter assay of a series of different fragments of cse promoter activity in raw264.7 cells that were treated with 100 or 500 µM Hcy for 12 h. Luciferase activities were expressed as fold increases over pGL3-basic vector. n = 7. * p
    L Alanyl L Alanine Lala, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l alanyl l alanine lala/product/Millipore
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    l alanyl l alanine lala - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    98
    TaKaRa bgl l
    Identification of the genome types of HAdV-B14 strains GZ01 (Guangzhou), BJ430 (Beijing), and CHN2012 (Beijing). The in silico restriction enzyme analysis (REA) digestion patterns of HAdV-14 genomes were generated using the Vector NTI 10.3.0 software (Invitrogen Corp., San Diego, CA, USA). Genome digestion patterns of the strains are as follows: ( A ) de Wit or prototype (AY803294); ( B ) GZ01 (JQ824845); ( C ) 303600, reported as HAdV-B14p1 (FJ822614); ( D ) BJ430 (JN032132); and ( E ) CHN2012 (JX892927). These were analyzed with Bam HI, <t>Bcl</t> I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal I, Sma I, Xba I, and Xho I, as described by Li et al. 59 All the three recent China outbreak genomes correspond to the genome type of HAdV-14p1 and have identical REA profiles to each other. They are divergent from the HAdV-B14 prototype (1955). M: 1 kb DNA Sizing Ladder.
    Bgl L, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bgl l/product/TaKaRa
    Average 98 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bgl l - by Bioz Stars, 2020-04
    98/100 stars
      Buy from Supplier

    91
    Millipore l dops
    Identification of the genome types of HAdV-B14 strains GZ01 (Guangzhou), BJ430 (Beijing), and CHN2012 (Beijing). The in silico restriction enzyme analysis (REA) digestion patterns of HAdV-14 genomes were generated using the Vector NTI 10.3.0 software (Invitrogen Corp., San Diego, CA, USA). Genome digestion patterns of the strains are as follows: ( A ) de Wit or prototype (AY803294); ( B ) GZ01 (JQ824845); ( C ) 303600, reported as HAdV-B14p1 (FJ822614); ( D ) BJ430 (JN032132); and ( E ) CHN2012 (JX892927). These were analyzed with Bam HI, <t>Bcl</t> I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal I, Sma I, Xba I, and Xho I, as described by Li et al. 59 All the three recent China outbreak genomes correspond to the genome type of HAdV-14p1 and have identical REA profiles to each other. They are divergent from the HAdV-B14 prototype (1955). M: 1 kb DNA Sizing Ladder.
    L Dops, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l dops/product/Millipore
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    l dops - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    99
    Millipore l l leucine
    Identification of the genome types of HAdV-B14 strains GZ01 (Guangzhou), BJ430 (Beijing), and CHN2012 (Beijing). The in silico restriction enzyme analysis (REA) digestion patterns of HAdV-14 genomes were generated using the Vector NTI 10.3.0 software (Invitrogen Corp., San Diego, CA, USA). Genome digestion patterns of the strains are as follows: ( A ) de Wit or prototype (AY803294); ( B ) GZ01 (JQ824845); ( C ) 303600, reported as HAdV-B14p1 (FJ822614); ( D ) BJ430 (JN032132); and ( E ) CHN2012 (JX892927). These were analyzed with Bam HI, <t>Bcl</t> I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal I, Sma I, Xba I, and Xho I, as described by Li et al. 59 All the three recent China outbreak genomes correspond to the genome type of HAdV-14p1 and have identical REA profiles to each other. They are divergent from the HAdV-B14 prototype (1955). M: 1 kb DNA Sizing Ladder.
    L L Leucine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l l leucine/product/Millipore
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    l l leucine - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore l glycyl l histidyl l lysine
    Identification of the genome types of HAdV-B14 strains GZ01 (Guangzhou), BJ430 (Beijing), and CHN2012 (Beijing). The in silico restriction enzyme analysis (REA) digestion patterns of HAdV-14 genomes were generated using the Vector NTI 10.3.0 software (Invitrogen Corp., San Diego, CA, USA). Genome digestion patterns of the strains are as follows: ( A ) de Wit or prototype (AY803294); ( B ) GZ01 (JQ824845); ( C ) 303600, reported as HAdV-B14p1 (FJ822614); ( D ) BJ430 (JN032132); and ( E ) CHN2012 (JX892927). These were analyzed with Bam HI, <t>Bcl</t> I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal I, Sma I, Xba I, and Xho I, as described by Li et al. 59 All the three recent China outbreak genomes correspond to the genome type of HAdV-14p1 and have identical REA profiles to each other. They are divergent from the HAdV-B14 prototype (1955). M: 1 kb DNA Sizing Ladder.
    L Glycyl L Histidyl L Lysine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l glycyl l histidyl l lysine/product/Millipore
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    l glycyl l histidyl l lysine - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore l carnitine l carnitine
    Identification of the genome types of HAdV-B14 strains GZ01 (Guangzhou), BJ430 (Beijing), and CHN2012 (Beijing). The in silico restriction enzyme analysis (REA) digestion patterns of HAdV-14 genomes were generated using the Vector NTI 10.3.0 software (Invitrogen Corp., San Diego, CA, USA). Genome digestion patterns of the strains are as follows: ( A ) de Wit or prototype (AY803294); ( B ) GZ01 (JQ824845); ( C ) 303600, reported as HAdV-B14p1 (FJ822614); ( D ) BJ430 (JN032132); and ( E ) CHN2012 (JX892927). These were analyzed with Bam HI, <t>Bcl</t> I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal I, Sma I, Xba I, and Xho I, as described by Li et al. 59 All the three recent China outbreak genomes correspond to the genome type of HAdV-14p1 and have identical REA profiles to each other. They are divergent from the HAdV-B14 prototype (1955). M: 1 kb DNA Sizing Ladder.
    L Carnitine L Carnitine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l carnitine l carnitine/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l carnitine l carnitine - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore l isoleucine l iso
    Identification of the genome types of HAdV-B14 strains GZ01 (Guangzhou), BJ430 (Beijing), and CHN2012 (Beijing). The in silico restriction enzyme analysis (REA) digestion patterns of HAdV-14 genomes were generated using the Vector NTI 10.3.0 software (Invitrogen Corp., San Diego, CA, USA). Genome digestion patterns of the strains are as follows: ( A ) de Wit or prototype (AY803294); ( B ) GZ01 (JQ824845); ( C ) 303600, reported as HAdV-B14p1 (FJ822614); ( D ) BJ430 (JN032132); and ( E ) CHN2012 (JX892927). These were analyzed with Bam HI, <t>Bcl</t> I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal I, Sma I, Xba I, and Xho I, as described by Li et al. 59 All the three recent China outbreak genomes correspond to the genome type of HAdV-14p1 and have identical REA profiles to each other. They are divergent from the HAdV-B14 prototype (1955). M: 1 kb DNA Sizing Ladder.
    L Isoleucine L Iso, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l isoleucine l iso/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    l isoleucine l iso - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore l tryptophan l trp
    Time course of <t>D-KYN</t> levels after systemic <t>D-TRP</t> administration in mice. D-KYN was determined in forebrain (a) and cerebellum (b) 30, 90 or 180 min after the D-TRP injection (300 mg/kg, i.p.). Data are the mean ± SEM (n = 4–5).
    L Tryptophan L Trp, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l tryptophan l trp/product/Millipore
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    l tryptophan l trp - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    90
    Tocris n acetyl l leucyl l leucyl l methional
    Time course of <t>D-KYN</t> levels after systemic <t>D-TRP</t> administration in mice. D-KYN was determined in forebrain (a) and cerebellum (b) 30, 90 or 180 min after the D-TRP injection (300 mg/kg, i.p.). Data are the mean ± SEM (n = 4–5).
    N Acetyl L Leucyl L Leucyl L Methional, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n acetyl l leucyl l leucyl l methional/product/Tocris
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    n acetyl l leucyl l leucyl l methional - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    99
    Millipore l phenylalanine l phe
    Time course of <t>D-KYN</t> levels after systemic <t>D-TRP</t> administration in mice. D-KYN was determined in forebrain (a) and cerebellum (b) 30, 90 or 180 min after the D-TRP injection (300 mg/kg, i.p.). Data are the mean ± SEM (n = 4–5).
    L Phenylalanine L Phe, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l phenylalanine l phe/product/Millipore
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    l phenylalanine l phe - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore l sulforaphane l sfn
    Effects of chemopreventive natural compounds on NF-κB promoter activity. Graphs representing the effect on NF-κB induction in MDA-MB-231 cells treated with different concentration of resveratrol (4.A), <t>sulphoraphane</t> (4.B), curcumin (4.C) and betulinic acid (4.D) (* p value
    L Sulforaphane L Sfn, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l sulforaphane l sfn/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    l sulforaphane l sfn - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore mm l l glutamine
    Effects of chemopreventive natural compounds on NF-κB promoter activity. Graphs representing the effect on NF-κB induction in MDA-MB-231 cells treated with different concentration of resveratrol (4.A), <t>sulphoraphane</t> (4.B), curcumin (4.C) and betulinic acid (4.D) (* p value
    Mm L L Glutamine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mm l l glutamine/product/Millipore
    Average 99 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    mm l l glutamine - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore l fuc l fucose
    Effects of chemopreventive natural compounds on NF-κB promoter activity. Graphs representing the effect on NF-κB induction in MDA-MB-231 cells treated with different concentration of resveratrol (4.A), <t>sulphoraphane</t> (4.B), curcumin (4.C) and betulinic acid (4.D) (* p value
    L Fuc L Fucose, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l fuc l fucose/product/Millipore
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    l fuc l fucose - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    97
    Millipore pha l
    Effects of chemopreventive natural compounds on NF-κB promoter activity. Graphs representing the effect on NF-κB induction in MDA-MB-231 cells treated with different concentration of resveratrol (4.A), <t>sulphoraphane</t> (4.B), curcumin (4.C) and betulinic acid (4.D) (* p value
    Pha L, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pha l/product/Millipore
    Average 97 stars, based on 473 article reviews
    Price from $9.99 to $1999.99
    pha l - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    99
    Millipore l t4
    Hypothyroidism is not a major determinant of the severe neonatal diabetes and lethality in Glis3 −/− mice. (A) Serum total T4 levels of maternal Glis3 +/+ and Glis3 +/− mice at midgestation (N = 5 to 8). (B) Survival rate of Glis3 +/+ and Glis3 −/− mice treated daily with <t>L-T4</t> (0.4 µg/g body weight) or vehicle. N = 10 to 12 mice for each group.
    L T4, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l t4/product/Millipore
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    l t4 - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore l kynurenine l kyn
    Treatment with <t>L-Kynurenine</t> fails to inhibit in vivo T CD8 responses to T Ag. WT mice were treated with L-Kyn (30 mg total as described in Materials and Methods) or PBS, and injected i.p. with C57SV cells. Nine days later, splenic T CD8 were examined for IFN-γ accumulation following restimulation with C57SV cells or synthetic peptides corresponding to T Ag epitopes. T Ag-specific T CD8 frequencies were determined after subtracting background and expressed as mean ± SEM of 6 L-Kyn-treated and 8 vehicle-treated mice (A). These values were used to calculate the absolute number of T Ag-specific T CD8 present within each spleen (B).
    L Kynurenine L Kyn, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l kynurenine l kyn/product/Millipore
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    l kynurenine l kyn - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Diagram representing distributions of the penetrations by segment of the D2 barrel for cells used for analysis in subsequent figures for d -AP5 ( left ) and l -AP5 animals ( right ). The penetrations in each group of animals were quite evenly spread through

    Journal: The Journal of Neuroscience

    Article Title: Experience-Dependent Plasticity of Adult Rat S1 Cortex Requires Local NMDA Receptor Activation

    doi: 10.1523/JNEUROSCI.18-23-10196.1998

    Figure Lengend Snippet: Diagram representing distributions of the penetrations by segment of the D2 barrel for cells used for analysis in subsequent figures for d -AP5 ( left ) and l -AP5 animals ( right ). The penetrations in each group of animals were quite evenly spread through

    Article Snippet: Both d -AP5 and l -AP5 were purchased from Tocris.

    Techniques:

    Suppression of whisker-driven responses of cells in layers I–IV after perfusion of barrel cortex with d -AP5 at concentrations of 0.1–10 m m . Epidural perfusion was maintained for a minimum of 6 hr. Findings are from 12 animals tested at

    Journal: The Journal of Neuroscience

    Article Title: Experience-Dependent Plasticity of Adult Rat S1 Cortex Requires Local NMDA Receptor Activation

    doi: 10.1523/JNEUROSCI.18-23-10196.1998

    Figure Lengend Snippet: Suppression of whisker-driven responses of cells in layers I–IV after perfusion of barrel cortex with d -AP5 at concentrations of 0.1–10 m m . Epidural perfusion was maintained for a minimum of 6 hr. Findings are from 12 animals tested at

    Article Snippet: Both d -AP5 and l -AP5 were purchased from Tocris.

    Techniques: Whisker Assay

    Top , Distribution of spike durations recorded from d -AP5-perfused animals for the cells in layers II–III ( black bars ) and IV ( gray bars ) of the D2 barrel column used for this study. Note the bimodal nature of the histogram with antimode at 0.75

    Journal: The Journal of Neuroscience

    Article Title: Experience-Dependent Plasticity of Adult Rat S1 Cortex Requires Local NMDA Receptor Activation

    doi: 10.1523/JNEUROSCI.18-23-10196.1998

    Figure Lengend Snippet: Top , Distribution of spike durations recorded from d -AP5-perfused animals for the cells in layers II–III ( black bars ) and IV ( gray bars ) of the D2 barrel column used for this study. Note the bimodal nature of the histogram with antimode at 0.75

    Article Snippet: Both d -AP5 and l -AP5 were purchased from Tocris.

    Techniques:

    Suppression of long-latency responses by perfusion of 500 μ m d -AP5 onto cortex. Cells A and B are two neurons analyzed from barrel D4. Cell A shows changes in mean response magnitudes to center whisker D4 and to surround whiskers D3 and D5 during

    Journal: The Journal of Neuroscience

    Article Title: Experience-Dependent Plasticity of Adult Rat S1 Cortex Requires Local NMDA Receptor Activation

    doi: 10.1523/JNEUROSCI.18-23-10196.1998

    Figure Lengend Snippet: Suppression of long-latency responses by perfusion of 500 μ m d -AP5 onto cortex. Cells A and B are two neurons analyzed from barrel D4. Cell A shows changes in mean response magnitudes to center whisker D4 and to surround whiskers D3 and D5 during

    Article Snippet: Both d -AP5 and l -AP5 were purchased from Tocris.

    Techniques: Whisker Assay

    Analysis of base editing patterns and efficiencies in single yeast colonies selected for canavanine resistance. A comparison of base editing frequencies for nCDA1-BE3, cCDA1-BE3, and selected base editors with truncated CDA1 domains is shown. Yeast cells were transformed with plasmids expressing the base editor and an sgRNA targeting the Can1–5 site. The target sequence is shown with the cytidines that can potentially undergo editing in red and the PAM in blue. If C-to-T conversion occurs at position −18 or −19 or both, the Can1 gene will be inactivated and the cell becomes resistant to canavanine. Values and error bars reflect the mean and standard deviation of three biological replicates. Source data are provided as a Source Data file. See also Table 1

    Journal: Nature Communications

    Article Title: Engineering of high-precision base editors for site-specific single nucleotide replacement

    doi: 10.1038/s41467-018-08034-8

    Figure Lengend Snippet: Analysis of base editing patterns and efficiencies in single yeast colonies selected for canavanine resistance. A comparison of base editing frequencies for nCDA1-BE3, cCDA1-BE3, and selected base editors with truncated CDA1 domains is shown. Yeast cells were transformed with plasmids expressing the base editor and an sgRNA targeting the Can1–5 site. The target sequence is shown with the cytidines that can potentially undergo editing in red and the PAM in blue. If C-to-T conversion occurs at position −18 or −19 or both, the Can1 gene will be inactivated and the cell becomes resistant to canavanine. Values and error bars reflect the mean and standard deviation of three biological replicates. Source data are provided as a Source Data file. See also Table 1

    Article Snippet: The cells were incubated for 20 h prior to plating on YPAD rich or SC media plates without arginine but with 60 mg/mL l -canavanine (Sigma).

    Techniques: Transformation Assay, Expressing, Sequencing, Standard Deviation

    Rigid linkers narrow the width of the editing window of BE3. a Protospacers and PAM (blue) sequences of the genomic loci tested, with the target Cs shown in red. Subscript numbers indicate the positions of the cytidines relative to the PAM. C-to-T editing at any of the indicated Cs inactivates the Can1 transporter and thus causes resistance to canavanine 17 . b Editing efficiency and specificity of the base editors tested as determined by canavanine selection. The x -axis represents the target Cs within the protospacers. The y -axis shows their C-to-T editing frequency (see Methods and Supplementary Figure 1 ). Sequences of canavanine-resistant mutants aligned with the corresponding reference sequences are shown in Supplementary Figure 3 . Values and error bars represent the mean and standard deviation of three independent biological replicates. Source data are provided as a Source Data file

    Journal: Nature Communications

    Article Title: Engineering of high-precision base editors for site-specific single nucleotide replacement

    doi: 10.1038/s41467-018-08034-8

    Figure Lengend Snippet: Rigid linkers narrow the width of the editing window of BE3. a Protospacers and PAM (blue) sequences of the genomic loci tested, with the target Cs shown in red. Subscript numbers indicate the positions of the cytidines relative to the PAM. C-to-T editing at any of the indicated Cs inactivates the Can1 transporter and thus causes resistance to canavanine 17 . b Editing efficiency and specificity of the base editors tested as determined by canavanine selection. The x -axis represents the target Cs within the protospacers. The y -axis shows their C-to-T editing frequency (see Methods and Supplementary Figure 1 ). Sequences of canavanine-resistant mutants aligned with the corresponding reference sequences are shown in Supplementary Figure 3 . Values and error bars represent the mean and standard deviation of three independent biological replicates. Source data are provided as a Source Data file

    Article Snippet: The cells were incubated for 20 h prior to plating on YPAD rich or SC media plates without arginine but with 60 mg/mL l -canavanine (Sigma).

    Techniques: Selection, Standard Deviation

    Gel retardation analysis to evaluate the inhibitory effects of rhamnulose-1-phophate (A) and the phosphorylated rhamnulose produced by the His-RhaB-catalyzed reaction (B) on the DNA binding of RhaR. The 32 P-labeled P rhaEW probe (0.02 pmol) and the RhaR

    Journal: Journal of Bacteriology

    Article Title: Regulation of the rhaEWRBMA Operon Involved in l-Rhamnose Catabolism through Two Transcriptional Factors, RhaR and CcpA, in Bacillus subtilis

    doi: 10.1128/JB.00856-15

    Figure Lengend Snippet: Gel retardation analysis to evaluate the inhibitory effects of rhamnulose-1-phophate (A) and the phosphorylated rhamnulose produced by the His-RhaB-catalyzed reaction (B) on the DNA binding of RhaR. The 32 P-labeled P rhaEW probe (0.02 pmol) and the RhaR

    Article Snippet: By measuring the absorbance of the colored mixture at 545 nm, the amount of ketoses converted from rhamnose was quantified, based on the molar extinction coefficient at 545 nm (1,530 M−1 cm−1 ) determined by using l -rhamnulose (Sigma-Aldrich), which was dissolved at various concentrations with 50 mM Tris-Cl buffer (pH 8.0) plus 1 mM MnCl2 , and colored by the same treatment for spectrophotometric measurement.

    Techniques: Electrophoretic Mobility Shift Assay, Produced, Binding Assay, Labeling

    Effects of VAEI-treated DC on T cells. Purified human CD4 + T lymphocytes were cultured in the presence of medium (NC) or stimulated with phytohemagglutinin-L (PC; 10 μg/ml). Unstimulated T cells were further co-cultivated with immature DC (DC, CD4 + ), DC treated with a maturation cocktail (DC Stim, CD4 + ; 500 ng/mL LPS; 50 ng/mL TNF-alpha and 10 ng/mL IL-1beta), VAEI-treated DC (DC + VAEI, CD4 + ; Iscador® Qu Spez; 0.5 μg/ml), DC incubated with ML-depleted VAEI (DC + VAEI-ML, CD4 + ; Iscador®; 0.66 μg/ml) or VAEI and anti-ML antibody (DC + VAEI + aML-Ab, CD4 + ; 2.5 μg/ml). (A) Cell proliferation analysis was done using CFSE staining and flow cytometry. (B) CD25 surface marker expression was analyzed as a second indicator for T cell activation. Data of 6 individual experiments are presented as mean ± SD in relation to untreated T cells (NC) or untreated T cells co-cultured with immature DC (DC, CD4 + ). (C) IFN-γ release by T cells was analyzed in the supernatants of the co-cultures of 6 independent experiments using a cytokine bead array assay. Asterisks indicate significant differences between the groups (*P

    Journal: PLoS ONE

    Article Title: Viscum album neutralizes tumor-induced immunosuppression in a human in vitro cell model

    doi: 10.1371/journal.pone.0181553

    Figure Lengend Snippet: Effects of VAEI-treated DC on T cells. Purified human CD4 + T lymphocytes were cultured in the presence of medium (NC) or stimulated with phytohemagglutinin-L (PC; 10 μg/ml). Unstimulated T cells were further co-cultivated with immature DC (DC, CD4 + ), DC treated with a maturation cocktail (DC Stim, CD4 + ; 500 ng/mL LPS; 50 ng/mL TNF-alpha and 10 ng/mL IL-1beta), VAEI-treated DC (DC + VAEI, CD4 + ; Iscador® Qu Spez; 0.5 μg/ml), DC incubated with ML-depleted VAEI (DC + VAEI-ML, CD4 + ; Iscador®; 0.66 μg/ml) or VAEI and anti-ML antibody (DC + VAEI + aML-Ab, CD4 + ; 2.5 μg/ml). (A) Cell proliferation analysis was done using CFSE staining and flow cytometry. (B) CD25 surface marker expression was analyzed as a second indicator for T cell activation. Data of 6 individual experiments are presented as mean ± SD in relation to untreated T cells (NC) or untreated T cells co-cultured with immature DC (DC, CD4 + ). (C) IFN-γ release by T cells was analyzed in the supernatants of the co-cultures of 6 independent experiments using a cytokine bead array assay. Asterisks indicate significant differences between the groups (*P

    Article Snippet: As controls, CFSE+ CD4+ T cells were cultured with medium alone (NC) or in the presence of phytohemagglutinin-L (PC; PHA-L; 10 μg/mL; Sigma-Aldrich, Taufkirchen, Germany).

    Techniques: Purification, Cell Culture, Incubation, Staining, Flow Cytometry, Cytometry, Marker, Expressing, Activation Assay

    Effects of VAEI-treated DC on T cells after tumor-induced immunosuppression. Purified human CD4 + T lymphocytes were cultured in the presence of medium (NC) or stimulated with phytohemagglutinin-L (PC; 10 μg/ml). Co-cultivation of T cells was carried out with unstimulated DC treated with 10% RPMI 1640 medium (CD4 + + DC + Medium), 10% tumor supernatant of an RCC line (CD4 + + DC + TS + ), TS and VAEI (CD4 + + DC + TS + VAEI; Iscador® Qu Spez; 0.5 μg/ml), TS and ML-depleted VAEI (CD4 + + DC + TS + VAEI-ML; Iscador®; 0.66 μg/ml) or TS, VAEI and anti-ML antibody (CD4 + + DC + TS + VAEI + aML-Ab, CD4 + ; 2.5 μg/ml). (A) Cell division analysis was done using CFSE staining and flow cytometry. (B) CD25 surface marker expression was analyzed as a second indicator for T cell activation. Data from 6 individual experiments are presented as mean ± SD in relation to untreated T cells (NC) or T cells co-cultured with TS-treated DC (DC + TS). (C) IFN-γ release by T cells was analyzed in the supernatants of 6 independent co-culture experiments using cytokine bead array assay. Asterisks indicate significant differences between the groups (*P

    Journal: PLoS ONE

    Article Title: Viscum album neutralizes tumor-induced immunosuppression in a human in vitro cell model

    doi: 10.1371/journal.pone.0181553

    Figure Lengend Snippet: Effects of VAEI-treated DC on T cells after tumor-induced immunosuppression. Purified human CD4 + T lymphocytes were cultured in the presence of medium (NC) or stimulated with phytohemagglutinin-L (PC; 10 μg/ml). Co-cultivation of T cells was carried out with unstimulated DC treated with 10% RPMI 1640 medium (CD4 + + DC + Medium), 10% tumor supernatant of an RCC line (CD4 + + DC + TS + ), TS and VAEI (CD4 + + DC + TS + VAEI; Iscador® Qu Spez; 0.5 μg/ml), TS and ML-depleted VAEI (CD4 + + DC + TS + VAEI-ML; Iscador®; 0.66 μg/ml) or TS, VAEI and anti-ML antibody (CD4 + + DC + TS + VAEI + aML-Ab, CD4 + ; 2.5 μg/ml). (A) Cell division analysis was done using CFSE staining and flow cytometry. (B) CD25 surface marker expression was analyzed as a second indicator for T cell activation. Data from 6 individual experiments are presented as mean ± SD in relation to untreated T cells (NC) or T cells co-cultured with TS-treated DC (DC + TS). (C) IFN-γ release by T cells was analyzed in the supernatants of 6 independent co-culture experiments using cytokine bead array assay. Asterisks indicate significant differences between the groups (*P

    Article Snippet: As controls, CFSE+ CD4+ T cells were cultured with medium alone (NC) or in the presence of phytohemagglutinin-L (PC; PHA-L; 10 μg/mL; Sigma-Aldrich, Taufkirchen, Germany).

    Techniques: Purification, Cell Culture, Staining, Flow Cytometry, Cytometry, Marker, Expressing, Activation Assay, Co-Culture Assay

    Effects of Hcy on CSE expression and promoter activity in raw264.7 macrophages. ( A – D ) Hcy down-regulated CSE mRNA and protein expressions in raw264.7 macrophages in dose- and time-dependent manners ( E , F ). Effect of l -homocystine and l -cysteine on CSE protein expression. CSE protein expression was evaluated by immunoblotting analysis while CSE mRNA level determined by quantitative PCR. GAPDH protein and 18S mRNA served as loading controls. Mean ± SEM, n = 3–8. ( G ) Reporter assay of a series of different fragments of cse promoter activity in raw264.7 cells that were treated with 100 or 500 µM Hcy for 12 h. Luciferase activities were expressed as fold increases over pGL3-basic vector. n = 7. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Homocysteine Triggers Inflammatory Responses in Macrophages through Inhibiting CSE-H2S Signaling via DNA Hypermethylation of CSE Promoter

    doi: 10.3390/ijms160612560

    Figure Lengend Snippet: Effects of Hcy on CSE expression and promoter activity in raw264.7 macrophages. ( A – D ) Hcy down-regulated CSE mRNA and protein expressions in raw264.7 macrophages in dose- and time-dependent manners ( E , F ). Effect of l -homocystine and l -cysteine on CSE protein expression. CSE protein expression was evaluated by immunoblotting analysis while CSE mRNA level determined by quantitative PCR. GAPDH protein and 18S mRNA served as loading controls. Mean ± SEM, n = 3–8. ( G ) Reporter assay of a series of different fragments of cse promoter activity in raw264.7 cells that were treated with 100 or 500 µM Hcy for 12 h. Luciferase activities were expressed as fold increases over pGL3-basic vector. n = 7. * p

    Article Snippet: Methionine, PAG, l -Homocysteine, l -homocystine, l -cysteine, Aza, and other chemicals unless specified were purchased from Sigma (St. Louis, MO, USA).

    Techniques: Expressing, Activity Assay, Real-time Polymerase Chain Reaction, Reporter Assay, Luciferase, Plasmid Preparation

    Identification of the genome types of HAdV-B14 strains GZ01 (Guangzhou), BJ430 (Beijing), and CHN2012 (Beijing). The in silico restriction enzyme analysis (REA) digestion patterns of HAdV-14 genomes were generated using the Vector NTI 10.3.0 software (Invitrogen Corp., San Diego, CA, USA). Genome digestion patterns of the strains are as follows: ( A ) de Wit or prototype (AY803294); ( B ) GZ01 (JQ824845); ( C ) 303600, reported as HAdV-B14p1 (FJ822614); ( D ) BJ430 (JN032132); and ( E ) CHN2012 (JX892927). These were analyzed with Bam HI, Bcl I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal I, Sma I, Xba I, and Xho I, as described by Li et al. 59 All the three recent China outbreak genomes correspond to the genome type of HAdV-14p1 and have identical REA profiles to each other. They are divergent from the HAdV-B14 prototype (1955). M: 1 kb DNA Sizing Ladder.

    Journal: Emerging Microbes & Infections

    Article Title: Comparative genomic analysis of two emergent human adenovirus type 14 respiratory pathogen isolates in China reveals similar yet divergent genomes

    doi: 10.1038/emi.2017.78

    Figure Lengend Snippet: Identification of the genome types of HAdV-B14 strains GZ01 (Guangzhou), BJ430 (Beijing), and CHN2012 (Beijing). The in silico restriction enzyme analysis (REA) digestion patterns of HAdV-14 genomes were generated using the Vector NTI 10.3.0 software (Invitrogen Corp., San Diego, CA, USA). Genome digestion patterns of the strains are as follows: ( A ) de Wit or prototype (AY803294); ( B ) GZ01 (JQ824845); ( C ) 303600, reported as HAdV-B14p1 (FJ822614); ( D ) BJ430 (JN032132); and ( E ) CHN2012 (JX892927). These were analyzed with Bam HI, Bcl I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal I, Sma I, Xba I, and Xho I, as described by Li et al. 59 All the three recent China outbreak genomes correspond to the genome type of HAdV-14p1 and have identical REA profiles to each other. They are divergent from the HAdV-B14 prototype (1955). M: 1 kb DNA Sizing Ladder.

    Article Snippet: Genome type identification In silico restriction maps of available genome sequences from all HAdV-14 strains CHN/GZ01/2010, 303600, CHN/BJ430, CHN2012, and prototype de Wit were generated using the Vector NTI 10.3.0 software (Invitrogen Corp.; San Diego, CA, USA) as described in earlier studies., , These included profiles from restriction enzymes Bam HI, Bcl I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal l, Sma I, Xba I, and Xho I (TaKaRa Corp.; China), all of which were chosen in order to be consistent with the original nomenclature system devised by Li and colleagues.

    Techniques: In Silico, Generated, Plasmid Preparation, Software

    Time course of D-KYN levels after systemic D-TRP administration in mice. D-KYN was determined in forebrain (a) and cerebellum (b) 30, 90 or 180 min after the D-TRP injection (300 mg/kg, i.p.). Data are the mean ± SEM (n = 4–5).

    Journal: Journal of neurochemistry

    Article Title: Role of D-amino acid oxidase in the production of kynurenine pathway metabolites from D-tryptophan in mice

    doi: 10.1111/jnc.13455

    Figure Lengend Snippet: Time course of D-KYN levels after systemic D-TRP administration in mice. D-KYN was determined in forebrain (a) and cerebellum (b) 30, 90 or 180 min after the D-TRP injection (300 mg/kg, i.p.). Data are the mean ± SEM (n = 4–5).

    Article Snippet: D-Tryptophan (D-TRP), L-tryptophan (L-TRP), D-kynurenine (D-KYN), kynurenic acid (KYNA), 3-hydroxy-DL-kynurenine (3-HK), quinolinic acid (QUIN), D-amino acid oxidase (D-AAO, purified from porcine kidney; product number A5222) and 3-methylpyrazole-5-carboxylic acid (MPC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Mouse Assay, Injection

    Effect of D-AAO inhibition on KYNA production from D-TRP. KYNA was measured in liver (a), plasma (b), forebrain (c) and cerebellum (d) 90 min after the i.p. administration of saline, D-TRP (300 mg/kg) alone or with (MPC + D-TRP) pre-treatment (30 min) with the D-AAO inhibitor MPC (100 mg/kg i.p.). Data are the mean ± SEM (n = 5–7). *p

    Journal: Journal of neurochemistry

    Article Title: Role of D-amino acid oxidase in the production of kynurenine pathway metabolites from D-tryptophan in mice

    doi: 10.1111/jnc.13455

    Figure Lengend Snippet: Effect of D-AAO inhibition on KYNA production from D-TRP. KYNA was measured in liver (a), plasma (b), forebrain (c) and cerebellum (d) 90 min after the i.p. administration of saline, D-TRP (300 mg/kg) alone or with (MPC + D-TRP) pre-treatment (30 min) with the D-AAO inhibitor MPC (100 mg/kg i.p.). Data are the mean ± SEM (n = 5–7). *p

    Article Snippet: D-Tryptophan (D-TRP), L-tryptophan (L-TRP), D-kynurenine (D-KYN), kynurenic acid (KYNA), 3-hydroxy-DL-kynurenine (3-HK), quinolinic acid (QUIN), D-amino acid oxidase (D-AAO, purified from porcine kidney; product number A5222) and 3-methylpyrazole-5-carboxylic acid (MPC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Inhibition

    Enzymatic degradation of D-TRP and L-TRP in vivo . The schematic diagram illustrates enzymatic processes involved in the degradation of L-TRP and D-TRP in mice under physiological conditions. IDO: indoleamine-2,3-dioxygenase; TDO: tryptophan-2,3-dioxygenase; D-AAO: D-amino acid oxidase; KAT: kynurenine aminotransferase; L:TRP: L-tryptophan; D-TRP: D-tryptophan; L-KYN: L-kynurenine; D-KYN: D-kynurenine; KYNA: kynurenic acid, 3-HK: 3-hydroxykynurenine; QUIN: quinolinic acid; KMO: kynurenine 3-monooxygenase.

    Journal: Journal of neurochemistry

    Article Title: Role of D-amino acid oxidase in the production of kynurenine pathway metabolites from D-tryptophan in mice

    doi: 10.1111/jnc.13455

    Figure Lengend Snippet: Enzymatic degradation of D-TRP and L-TRP in vivo . The schematic diagram illustrates enzymatic processes involved in the degradation of L-TRP and D-TRP in mice under physiological conditions. IDO: indoleamine-2,3-dioxygenase; TDO: tryptophan-2,3-dioxygenase; D-AAO: D-amino acid oxidase; KAT: kynurenine aminotransferase; L:TRP: L-tryptophan; D-TRP: D-tryptophan; L-KYN: L-kynurenine; D-KYN: D-kynurenine; KYNA: kynurenic acid, 3-HK: 3-hydroxykynurenine; QUIN: quinolinic acid; KMO: kynurenine 3-monooxygenase.

    Article Snippet: D-Tryptophan (D-TRP), L-tryptophan (L-TRP), D-kynurenine (D-KYN), kynurenic acid (KYNA), 3-hydroxy-DL-kynurenine (3-HK), quinolinic acid (QUIN), D-amino acid oxidase (D-AAO, purified from porcine kidney; product number A5222) and 3-methylpyrazole-5-carboxylic acid (MPC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: In Vivo, Mouse Assay

    Native Page analysis of proteins from the crude bacterial extract. The gels were stained for l - phenylalanine:2 - oxoglutarate ( a ), l - tyrosine:2 - oxoglutarate ( b ), l - tryptophan:2 - oxoglutarate ( c ) and l - aspartate:2 - oxoglutarate ( d ) activity

    Journal: Amino Acids

    Article Title: tyrB-2 and phhC genes of Pseudomonas putida encode aromatic amino acid aminotransferase isozymes: evidence at the protein level

    doi: 10.1007/s00726-013-1508-y

    Figure Lengend Snippet: Native Page analysis of proteins from the crude bacterial extract. The gels were stained for l - phenylalanine:2 - oxoglutarate ( a ), l - tyrosine:2 - oxoglutarate ( b ), l - tryptophan:2 - oxoglutarate ( c ) and l - aspartate:2 - oxoglutarate ( d ) activity

    Article Snippet: The fractions exhibiting l -tryptophan:2 -oxoglutarate activity were pooled and concentrated to a volume of approximately 12 ml using an Amicon concentrator (Millipore Corporation; Bedford, MA, USA) supplied with a PM 10 membrane.

    Techniques: Clear Native PAGE, Staining, Activity Assay

    Effects of chemopreventive natural compounds on NF-κB promoter activity. Graphs representing the effect on NF-κB induction in MDA-MB-231 cells treated with different concentration of resveratrol (4.A), sulphoraphane (4.B), curcumin (4.C) and betulinic acid (4.D) (* p value

    Journal: PLoS ONE

    Article Title: A New Multicolor Bioluminescence Imaging Platform to Investigate NF-?B Activity and Apoptosis in Human Breast Cancer Cells

    doi: 10.1371/journal.pone.0085550

    Figure Lengend Snippet: Effects of chemopreventive natural compounds on NF-κB promoter activity. Graphs representing the effect on NF-κB induction in MDA-MB-231 cells treated with different concentration of resveratrol (4.A), sulphoraphane (4.B), curcumin (4.C) and betulinic acid (4.D) (* p value

    Article Snippet: Compounds were purchased from the following companies: celastrol and curcumin (Cayman, Michigan, U.S.A.), L-sulforaphane and resveratrol (Sigma), and betulinic acid (Tocris, Bristol, U.K.).

    Techniques: Activity Assay, Multiple Displacement Amplification, Concentration Assay

    Effects of chemopreventive natural compounds on caspase 3/7-mediated apoptosis in vitro . Graph representing caspase 3/7 activity measured with the caspase 3/7 glo assay and corrected for cell vitality at 4 hours after treatment with celastrol (7.A) resveratrol (7.B), sulphoraphane (7.C), curcumin (7.D) and betulinic acid (7.E).

    Journal: PLoS ONE

    Article Title: A New Multicolor Bioluminescence Imaging Platform to Investigate NF-?B Activity and Apoptosis in Human Breast Cancer Cells

    doi: 10.1371/journal.pone.0085550

    Figure Lengend Snippet: Effects of chemopreventive natural compounds on caspase 3/7-mediated apoptosis in vitro . Graph representing caspase 3/7 activity measured with the caspase 3/7 glo assay and corrected for cell vitality at 4 hours after treatment with celastrol (7.A) resveratrol (7.B), sulphoraphane (7.C), curcumin (7.D) and betulinic acid (7.E).

    Article Snippet: Compounds were purchased from the following companies: celastrol and curcumin (Cayman, Michigan, U.S.A.), L-sulforaphane and resveratrol (Sigma), and betulinic acid (Tocris, Bristol, U.K.).

    Techniques: In Vitro, Activity Assay, Glo Assay

    Hypothyroidism is not a major determinant of the severe neonatal diabetes and lethality in Glis3 −/− mice. (A) Serum total T4 levels of maternal Glis3 +/+ and Glis3 +/− mice at midgestation (N = 5 to 8). (B) Survival rate of Glis3 +/+ and Glis3 −/− mice treated daily with L-T4 (0.4 µg/g body weight) or vehicle. N = 10 to 12 mice for each group.

    Journal: Endocrinology

    Article Title: Differential Gene Dosage Effects of Diabetes-Associated Gene GLIS3 in Pancreatic β Cell Differentiation and Function

    doi: 10.1210/en.2016-1541

    Figure Lengend Snippet: Hypothyroidism is not a major determinant of the severe neonatal diabetes and lethality in Glis3 −/− mice. (A) Serum total T4 levels of maternal Glis3 +/+ and Glis3 +/− mice at midgestation (N = 5 to 8). (B) Survival rate of Glis3 +/+ and Glis3 −/− mice treated daily with L-T4 (0.4 µg/g body weight) or vehicle. N = 10 to 12 mice for each group.

    Article Snippet: In l -thyroxine (L-T4) replacement experiment, we dissolved L-T4 (Sigma-Aldrich) in 1 M NaOH to make the stock solution at 100 mg/mL.

    Techniques: Mouse Assay

    Treatment with L-Kynurenine fails to inhibit in vivo T CD8 responses to T Ag. WT mice were treated with L-Kyn (30 mg total as described in Materials and Methods) or PBS, and injected i.p. with C57SV cells. Nine days later, splenic T CD8 were examined for IFN-γ accumulation following restimulation with C57SV cells or synthetic peptides corresponding to T Ag epitopes. T Ag-specific T CD8 frequencies were determined after subtracting background and expressed as mean ± SEM of 6 L-Kyn-treated and 8 vehicle-treated mice (A). These values were used to calculate the absolute number of T Ag-specific T CD8 present within each spleen (B).

    Journal: PLoS ONE

    Article Title: Suppression of Immunodominant Antitumor and Antiviral CD8+ T Cell Responses by Indoleamine 2,3-Dioxygenase

    doi: 10.1371/journal.pone.0090439

    Figure Lengend Snippet: Treatment with L-Kynurenine fails to inhibit in vivo T CD8 responses to T Ag. WT mice were treated with L-Kyn (30 mg total as described in Materials and Methods) or PBS, and injected i.p. with C57SV cells. Nine days later, splenic T CD8 were examined for IFN-γ accumulation following restimulation with C57SV cells or synthetic peptides corresponding to T Ag epitopes. T Ag-specific T CD8 frequencies were determined after subtracting background and expressed as mean ± SEM of 6 L-Kyn-treated and 8 vehicle-treated mice (A). These values were used to calculate the absolute number of T Ag-specific T CD8 present within each spleen (B).

    Article Snippet: L-kynurenine (L-Kyn), also from Sigma, was dissolved at 10 mg/mL in PBS shortly before injection.

    Techniques: In Vivo, Mouse Assay, Injection

    Kynurenine 3-monooxygenase mediates a deficit in Novel Object Recognition following peripheral immune challenge. LPS administration induces a significant reduction in preference for the novel object in WT mice, whereas KMO−/− mice are

    Journal: Brain, behavior, and immunity

    Article Title: Indoleamine 2,3-dioxygenase-dependent neurotoxic kynurenine metabolism mediates inflammation-induced deficit in recognition memory

    doi: 10.1016/j.bbi.2015.06.022

    Figure Lengend Snippet: Kynurenine 3-monooxygenase mediates a deficit in Novel Object Recognition following peripheral immune challenge. LPS administration induces a significant reduction in preference for the novel object in WT mice, whereas KMO−/− mice are

    Article Snippet: L-kynurenine (cat# K8265) was purchased from Sigma (St. Louis, MO) and prepared on the morning of injections.

    Techniques: Mouse Assay

    The kynurenine pathway of tryptophan metabolism. In the brain, kynurenine metabolism can begin with the catabolism of tryptophan to N-formylkynurenine via either tryptophan 2,3-dioxygenase (TDO) or indoleamine 2,3-dioxygenase (IDO), expressed in both

    Journal: Brain, behavior, and immunity

    Article Title: Indoleamine 2,3-dioxygenase-dependent neurotoxic kynurenine metabolism mediates inflammation-induced deficit in recognition memory

    doi: 10.1016/j.bbi.2015.06.022

    Figure Lengend Snippet: The kynurenine pathway of tryptophan metabolism. In the brain, kynurenine metabolism can begin with the catabolism of tryptophan to N-formylkynurenine via either tryptophan 2,3-dioxygenase (TDO) or indoleamine 2,3-dioxygenase (IDO), expressed in both

    Article Snippet: L-kynurenine (cat# K8265) was purchased from Sigma (St. Louis, MO) and prepared on the morning of injections.

    Techniques: