kv7 2 antibody Search Results


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    Alomone Labs anti kv7 2
    Hyper-SUMOylation of Kv1.1 and <t>Kv7.2</t> in SENP2 fxN/fxN Neurons
    Anti Kv7 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Alomone Labs rabbit anti kv7 2 antibody
    Inhibition of AMPK activity restored acute stress-induced decrease in membrane fraction of Kv7.3 subunit A and B: Gel images ( A ) and quantification of band density normalized by GAPDH ( B ) show the total protein levels of AMPK in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) of GAPDH-normalized band density of membrane fraction of <t>Kv7.2</t> and Kv7.3 in the PVN in stressed rats treated with ICV injection of vehicle and dorsomorphin (100 nmol in 10 µl aCSF). E and F , Original gel images ( E ) and quantification ( F ) of GAPDH-normalized band density of cytosolic fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with vehicle and dorsomorphin. The molecular weight was indicated on the right side of the gel images. In all the protein assays, 4 samples (each sample contains PVN tissues of 3 rats) were used in each group. *p
    Rabbit Anti Kv7 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti kv7 2 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti kv7 2 antibody - by Bioz Stars, 2022-10
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    80
    Antibodies Inc mouse monoclonal anti kv7 2 primary antibodies
    Inhibition of AMPK activity restored acute stress-induced decrease in membrane fraction of Kv7.3 subunit A and B: Gel images ( A ) and quantification of band density normalized by GAPDH ( B ) show the total protein levels of AMPK in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) of GAPDH-normalized band density of membrane fraction of <t>Kv7.2</t> and Kv7.3 in the PVN in stressed rats treated with ICV injection of vehicle and dorsomorphin (100 nmol in 10 µl aCSF). E and F , Original gel images ( E ) and quantification ( F ) of GAPDH-normalized band density of cytosolic fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with vehicle and dorsomorphin. The molecular weight was indicated on the right side of the gel images. In all the protein assays, 4 samples (each sample contains PVN tissues of 3 rats) were used in each group. *p
    Mouse Monoclonal Anti Kv7 2 Primary Antibodies, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti kv7 2 primary antibodies/product/Antibodies Inc
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti kv7 2 primary antibodies - by Bioz Stars, 2022-10
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    94
    Alomone Labs kv7 2
    Alterations in ion channel expression accompany decline in unit detection. A) Example of putative unit and LFP snippet from microelectrode arrays, accompanied by the quantified data (# of units and LFP amplitude) obtained from bi-weekly recording sessions across subjects (with standard error bars). Average LFP amplitude and # of units plotted on bar graphs for each time point. B) Averaged data within 0-40um for intensity ratios are plotted. <t>Nav1.6/Kv7.2</t> intensity ratio appears to coincide closest with unit detection over the 6 week time course, whereas Nav1.6/Kv4.3 ratio appears to best correspond to LFP amplitude over 6 weeks. In contrast, Nav1.6/Kv1.1 does not appear to correspond to either signal metric.
    Kv7 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kv7 2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kv7 2 - by Bioz Stars, 2022-10
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    Image Search Results


    Hyper-SUMOylation of Kv1.1 and Kv7.2 in SENP2 fxN/fxN Neurons

    Journal: Neuron

    Article Title: Hyper-SUMOylation of the Kv7 Potassium Channel Diminishes the M-Current Leading to Seizures and Sudden Death

    doi: 10.1016/j.neuron.2014.07.042

    Figure Lengend Snippet: Hyper-SUMOylation of Kv1.1 and Kv7.2 in SENP2 fxN/fxN Neurons

    Article Snippet: Western blotting was done with primary anti-SENP2 (Santa Cruz), anti-SUMO-1 (Cell Signaling), anti-SUMO-2 (Abacm), and anti-Kv7.2 (Alomone labs) antibodies.

    Techniques:

    Inhibition of AMPK activity restored acute stress-induced decrease in membrane fraction of Kv7.3 subunit A and B: Gel images ( A ) and quantification of band density normalized by GAPDH ( B ) show the total protein levels of AMPK in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) of GAPDH-normalized band density of membrane fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with ICV injection of vehicle and dorsomorphin (100 nmol in 10 µl aCSF). E and F , Original gel images ( E ) and quantification ( F ) of GAPDH-normalized band density of cytosolic fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with vehicle and dorsomorphin. The molecular weight was indicated on the right side of the gel images. In all the protein assays, 4 samples (each sample contains PVN tissues of 3 rats) were used in each group. *p

    Journal: Neuropharmacology

    Article Title: Acute Stress Diminishes M-current Contributing to Elevated Activity of Hypothalamic-Pituitary-Adrenal Axis

    doi: 10.1016/j.neuropharm.2016.11.024

    Figure Lengend Snippet: Inhibition of AMPK activity restored acute stress-induced decrease in membrane fraction of Kv7.3 subunit A and B: Gel images ( A ) and quantification of band density normalized by GAPDH ( B ) show the total protein levels of AMPK in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) of GAPDH-normalized band density of membrane fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with ICV injection of vehicle and dorsomorphin (100 nmol in 10 µl aCSF). E and F , Original gel images ( E ) and quantification ( F ) of GAPDH-normalized band density of cytosolic fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with vehicle and dorsomorphin. The molecular weight was indicated on the right side of the gel images. In all the protein assays, 4 samples (each sample contains PVN tissues of 3 rats) were used in each group. *p

    Article Snippet: The immunoblots were probed with a rabbit anti-Kv7.2 antibody (1:1000, Alomone Labs) and rabbit anti-Kv7.3 antibody (1:1000, Alomone Labs).

    Techniques: Inhibition, Activity Assay, Injection, Molecular Weight

    Acute stress diminished excitatory effect of M-current blocker and reduced M-current in PVN-CRH neurons ( A ): Confocal images show the distribution of Kv7.2 or Kv7.3 in the PVN-CRH neurons. B and C : Original recordings ( B ) and summary data ( C ) show the firing activity of eGFP-tagged PVN neurons in basal condition, the presence of Kv7 channel blocker XE-991 (3 µM), and washout in unstressed and acutely stressed rats (n = 20 neurons in unstressed and stressed rats, respectively). ( D) : Acute stress significantly reduced the increase in firing activity induced by XE-991. ( E) : Representative traces show deactivation tail currents in the absence (basal) or presence of Kv7 opener retigabine (10 µM) and blocker XE-991 (3 µM) in eGFP-tagged PVN neurons in stressed and unstressed rats. The deactivation tail currents were elicited by the voltage protocol shown in the insert. ( F ): summary data shows the basal and retigabine-induced M-currents, which were defined as XE-991-sensitive tail currents in basal and the presence of retigabine, were significantly diminished in the PVN-CRH neurons in stressed rats (n =15 neurons in 4 rats) compared with unstressed rats (n = 12 neurons in 4 rats).* p

    Journal: Neuropharmacology

    Article Title: Acute Stress Diminishes M-current Contributing to Elevated Activity of Hypothalamic-Pituitary-Adrenal Axis

    doi: 10.1016/j.neuropharm.2016.11.024

    Figure Lengend Snippet: Acute stress diminished excitatory effect of M-current blocker and reduced M-current in PVN-CRH neurons ( A ): Confocal images show the distribution of Kv7.2 or Kv7.3 in the PVN-CRH neurons. B and C : Original recordings ( B ) and summary data ( C ) show the firing activity of eGFP-tagged PVN neurons in basal condition, the presence of Kv7 channel blocker XE-991 (3 µM), and washout in unstressed and acutely stressed rats (n = 20 neurons in unstressed and stressed rats, respectively). ( D) : Acute stress significantly reduced the increase in firing activity induced by XE-991. ( E) : Representative traces show deactivation tail currents in the absence (basal) or presence of Kv7 opener retigabine (10 µM) and blocker XE-991 (3 µM) in eGFP-tagged PVN neurons in stressed and unstressed rats. The deactivation tail currents were elicited by the voltage protocol shown in the insert. ( F ): summary data shows the basal and retigabine-induced M-currents, which were defined as XE-991-sensitive tail currents in basal and the presence of retigabine, were significantly diminished in the PVN-CRH neurons in stressed rats (n =15 neurons in 4 rats) compared with unstressed rats (n = 12 neurons in 4 rats).* p

    Article Snippet: The immunoblots were probed with a rabbit anti-Kv7.2 antibody (1:1000, Alomone Labs) and rabbit anti-Kv7.3 antibody (1:1000, Alomone Labs).

    Techniques: Activity Assay

    Kv7.2 and Kv7.3 protein expression levels in the PVN in stressed and unstressed rats A and B , Representative blots ( A ) and quantification ( B ) show the total protein levels of Kv7.2 and Kv7.3 in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) show the membrane fraction protein levels of Kv7.2 and Kv7.3 in the PVN in unstressed and stressed rats. E and F , Original gel images ( E ) and quantification ( F ) show the cytosolic fraction protein levels of Kv7.2 and Kv7.3 in the PVN in unstressed and stressed rats (n = 4 samples, each sample contains PVN tissues of 3 rats) were used in each group. * p

    Journal: Neuropharmacology

    Article Title: Acute Stress Diminishes M-current Contributing to Elevated Activity of Hypothalamic-Pituitary-Adrenal Axis

    doi: 10.1016/j.neuropharm.2016.11.024

    Figure Lengend Snippet: Kv7.2 and Kv7.3 protein expression levels in the PVN in stressed and unstressed rats A and B , Representative blots ( A ) and quantification ( B ) show the total protein levels of Kv7.2 and Kv7.3 in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) show the membrane fraction protein levels of Kv7.2 and Kv7.3 in the PVN in unstressed and stressed rats. E and F , Original gel images ( E ) and quantification ( F ) show the cytosolic fraction protein levels of Kv7.2 and Kv7.3 in the PVN in unstressed and stressed rats (n = 4 samples, each sample contains PVN tissues of 3 rats) were used in each group. * p

    Article Snippet: The immunoblots were probed with a rabbit anti-Kv7.2 antibody (1:1000, Alomone Labs) and rabbit anti-Kv7.3 antibody (1:1000, Alomone Labs).

    Techniques: Expressing

    Alterations in ion channel expression accompany decline in unit detection. A) Example of putative unit and LFP snippet from microelectrode arrays, accompanied by the quantified data (# of units and LFP amplitude) obtained from bi-weekly recording sessions across subjects (with standard error bars). Average LFP amplitude and # of units plotted on bar graphs for each time point. B) Averaged data within 0-40um for intensity ratios are plotted. Nav1.6/Kv7.2 intensity ratio appears to coincide closest with unit detection over the 6 week time course, whereas Nav1.6/Kv4.3 ratio appears to best correspond to LFP amplitude over 6 weeks. In contrast, Nav1.6/Kv1.1 does not appear to correspond to either signal metric.

    Journal: bioRxiv

    Article Title: Alterations in Ion Channel Expression Surrounding Implanted Microelectrode Arrays in the Brain

    doi: 10.1101/518811

    Figure Lengend Snippet: Alterations in ion channel expression accompany decline in unit detection. A) Example of putative unit and LFP snippet from microelectrode arrays, accompanied by the quantified data (# of units and LFP amplitude) obtained from bi-weekly recording sessions across subjects (with standard error bars). Average LFP amplitude and # of units plotted on bar graphs for each time point. B) Averaged data within 0-40um for intensity ratios are plotted. Nav1.6/Kv7.2 intensity ratio appears to coincide closest with unit detection over the 6 week time course, whereas Nav1.6/Kv4.3 ratio appears to best correspond to LFP amplitude over 6 weeks. In contrast, Nav1.6/Kv1.1 does not appear to correspond to either signal metric.

    Article Snippet: Primary antibodies included rabbit anti-Nav1.6, −Kv1.1, −Kv4.3, and −Kv7.2 (1:200, Alomone Labs, Jerusalem, Israel).

    Techniques: Expressing

    Preliminary observations suggest Kv7.2 knockdown impacts excitatory synapses in culture. A) In vivo results of vesicular glutamate transporter 1 (VGLUT1), using data from a previous report 41 , show an elevation in VGLUT1 at 3 and 7 days. B) In vitro, cortical neurons transfected with Kv7.2 siRNA show successful transient knockdown of Kv7.2, a similar trend in VGLUT elevation at 3 and 7 days compared to in vivo expression, and an impact on PSD95 in the form of a reduction at 7 days. Taken together, these data suggest that the transient downregulation of Kv7.2 at 1 day in vivo ( Fig. 2A ) may be a mechanism for the upregulation of VGLUT1 at 3 and 7 days in vivo. Two biological replicates were performed for the preliminary in vitro data.

    Journal: bioRxiv

    Article Title: Alterations in Ion Channel Expression Surrounding Implanted Microelectrode Arrays in the Brain

    doi: 10.1101/518811

    Figure Lengend Snippet: Preliminary observations suggest Kv7.2 knockdown impacts excitatory synapses in culture. A) In vivo results of vesicular glutamate transporter 1 (VGLUT1), using data from a previous report 41 , show an elevation in VGLUT1 at 3 and 7 days. B) In vitro, cortical neurons transfected with Kv7.2 siRNA show successful transient knockdown of Kv7.2, a similar trend in VGLUT elevation at 3 and 7 days compared to in vivo expression, and an impact on PSD95 in the form of a reduction at 7 days. Taken together, these data suggest that the transient downregulation of Kv7.2 at 1 day in vivo ( Fig. 2A ) may be a mechanism for the upregulation of VGLUT1 at 3 and 7 days in vivo. Two biological replicates were performed for the preliminary in vitro data.

    Article Snippet: Primary antibodies included rabbit anti-Nav1.6, −Kv1.1, −Kv4.3, and −Kv7.2 (1:200, Alomone Labs, Jerusalem, Israel).

    Techniques: In Vivo, In Vitro, Transfection, Expressing