kv1 1 antibody Search Results


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    Alomone Labs kv1 1
    KCNE4 inhibition of heteromeric Kv1 channels. ( A ) Current traces from oocytes expressing <t>Kv1.1/Kv1.2,</t> Kv1.1/Kv1.3, and Kv1.2/Kv1.3 channels alone or together with KCNE4. Oocytes were clamped at −80 mV for 3 s, current traces were elicited by 2-s voltage steps for potentials ranging from −80 mV to +40 mV in 20-mV increments, and tail currents were recorded at −30 mV. ( B ) Mean current values of oocytes expressing Kv1 channels alone or of oocytes expressing Kv1 channels together with KCNE4. Peak current values, measured at +40 mV, were as follows: 26.1 ± 1.9 μ A ( n = 24) for Kv1.1/Kv1.2; 12.7 ± 1.7 μ A ( n = 24) for Kv1.1/Kv1.2/KCNE4; 21.9 ± 2.1 μ A ( n = 8) for Kv1.1/1.3; 2.5 ± 1.6 μ A ( n = 4) for Kv1.1/Kv1.3/KCNE4; 6.70 ± 0.70 μ A ( n = 11) for Kv1.2/Kv1.3; and 1.45 ± 0.30 μ A ( n = 12) for Kv1.2/Kv1.3/KCNE4. ( C ) Dose-response curve of charybdotoxin (ChTX) on Kv1.2- and Kv1.1/Kv1.2-expressing oocytes. Currents were measured at +60 mV. All data points are an average of four recordings on independent oocytes. ( D ) Current level of Kv1.2 and Kv1.2/Kv1.1SYG expressing oocytes measured at +60 mV.
    Kv1 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Antibodies Inc kv1 1
    Summary of the role of <t>Kv1.1</t> channels in regulating early postnatal neurogenesis in SGZ. The membrane potentials in type 1 radial glia-like neural stem cells and type 2a transit-amplifying progenitor cells are relatively hyperpolarized. In type 2b neural progenitors, cells lacking the Kv1.1 channel are more depolarized than wild types, further stimulating the proliferation of type 2b cells via activating the TrkB signaling pathway. The mature granule cells become hyperpolarized again.
    Kv1 1, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NeuroMab kv1 1
    Kv1 antibodies target intracellular epitopes. (A) Twenty-seven of the remaining 62 patients with unknown VGKC complex antigenic targets precipitated either 125 I-αDTX-labelled <t>Kv1.1/Kv1.2/Kv1.6</t> co-transfected HEK cell extracts (red circles) or
    Kv1 1, supplied by NeuroMab, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NeuroMab mouse anti kv1 1
    Kv1 antibodies target intracellular epitopes. (A) Twenty-seven of the remaining 62 patients with unknown VGKC complex antigenic targets precipitated either 125 I-αDTX-labelled <t>Kv1.1/Kv1.2/Kv1.6</t> co-transfected HEK cell extracts (red circles) or
    Mouse Anti Kv1 1, supplied by NeuroMab, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    KCNE4 inhibition of heteromeric Kv1 channels. ( A ) Current traces from oocytes expressing Kv1.1/Kv1.2, Kv1.1/Kv1.3, and Kv1.2/Kv1.3 channels alone or together with KCNE4. Oocytes were clamped at −80 mV for 3 s, current traces were elicited by 2-s voltage steps for potentials ranging from −80 mV to +40 mV in 20-mV increments, and tail currents were recorded at −30 mV. ( B ) Mean current values of oocytes expressing Kv1 channels alone or of oocytes expressing Kv1 channels together with KCNE4. Peak current values, measured at +40 mV, were as follows: 26.1 ± 1.9 μ A ( n = 24) for Kv1.1/Kv1.2; 12.7 ± 1.7 μ A ( n = 24) for Kv1.1/Kv1.2/KCNE4; 21.9 ± 2.1 μ A ( n = 8) for Kv1.1/1.3; 2.5 ± 1.6 μ A ( n = 4) for Kv1.1/Kv1.3/KCNE4; 6.70 ± 0.70 μ A ( n = 11) for Kv1.2/Kv1.3; and 1.45 ± 0.30 μ A ( n = 12) for Kv1.2/Kv1.3/KCNE4. ( C ) Dose-response curve of charybdotoxin (ChTX) on Kv1.2- and Kv1.1/Kv1.2-expressing oocytes. Currents were measured at +60 mV. All data points are an average of four recordings on independent oocytes. ( D ) Current level of Kv1.2 and Kv1.2/Kv1.1SYG expressing oocytes measured at +60 mV.

    Journal: Biophysical Journal

    Article Title: KCNE4 Is an Inhibitory Subunit to Kv1.1 and Kv1.3 Potassium Channels

    doi:

    Figure Lengend Snippet: KCNE4 inhibition of heteromeric Kv1 channels. ( A ) Current traces from oocytes expressing Kv1.1/Kv1.2, Kv1.1/Kv1.3, and Kv1.2/Kv1.3 channels alone or together with KCNE4. Oocytes were clamped at −80 mV for 3 s, current traces were elicited by 2-s voltage steps for potentials ranging from −80 mV to +40 mV in 20-mV increments, and tail currents were recorded at −30 mV. ( B ) Mean current values of oocytes expressing Kv1 channels alone or of oocytes expressing Kv1 channels together with KCNE4. Peak current values, measured at +40 mV, were as follows: 26.1 ± 1.9 μ A ( n = 24) for Kv1.1/Kv1.2; 12.7 ± 1.7 μ A ( n = 24) for Kv1.1/Kv1.2/KCNE4; 21.9 ± 2.1 μ A ( n = 8) for Kv1.1/1.3; 2.5 ± 1.6 μ A ( n = 4) for Kv1.1/Kv1.3/KCNE4; 6.70 ± 0.70 μ A ( n = 11) for Kv1.2/Kv1.3; and 1.45 ± 0.30 μ A ( n = 12) for Kv1.2/Kv1.3/KCNE4. ( C ) Dose-response curve of charybdotoxin (ChTX) on Kv1.2- and Kv1.1/Kv1.2-expressing oocytes. Currents were measured at +60 mV. All data points are an average of four recordings on independent oocytes. ( D ) Current level of Kv1.2 and Kv1.2/Kv1.1SYG expressing oocytes measured at +60 mV.

    Article Snippet: The cells were cotransfected with Kv1.1 or Kv1.1/KCNE4-c-myc, stained with antibodies raised against Kv1.1 channels and the c-myc epitop, and detected by confocal microscopy ( ).

    Techniques: Inhibition, Expressing

    KCNE4 inhibits Kv1.1 and Kv1.3 currents in HEK293 cells. ( A ) Current traces from whole-cell recordings ( left side ) and averaged IV-curves ( right side ) for HEK293 cells expressing Kv1.1 or Kv1.3 alone or together with KCNE4. Transfected cells were clamped at −80 mV for 3 s, current traces were elicited by 2-s voltage steps to potentials ranging from −100 mV to +60 mV in 20-mV increments, and tail currents were recorded at −30 mV (see inserted protocol). Cells expressing Kv1.1 and Kv1.3 channels responded to this voltage protocol with a fast-activating and a slow-inactivating current. For cells expressing KCNE4 together with Kv1.1 or Kv1.3 α -subunits, a significant reduction in the overall current level was observed. ( B ) Mean current values of HEK293 cells expressing either Kv1.1 or Kv1.3 alone or together with KCNE4. Peak current values were measured at +60 mV. For Kv1.1 and Kv1.1/KCNE4 an average current density of 195 ± 39 pA/pF ( n = 18) and 75 ± 17 pA/pF ( n = 19), respectively, was found. An even more pronounced reduction in the overall current level was observed for Kv1.3 and Kv1.3/KCNE4 with values of 91 ± 21 pA/pF ( n = 9) and 13 ± 4 pA/pF ( n = 12), respectively.

    Journal: Biophysical Journal

    Article Title: KCNE4 Is an Inhibitory Subunit to Kv1.1 and Kv1.3 Potassium Channels

    doi:

    Figure Lengend Snippet: KCNE4 inhibits Kv1.1 and Kv1.3 currents in HEK293 cells. ( A ) Current traces from whole-cell recordings ( left side ) and averaged IV-curves ( right side ) for HEK293 cells expressing Kv1.1 or Kv1.3 alone or together with KCNE4. Transfected cells were clamped at −80 mV for 3 s, current traces were elicited by 2-s voltage steps to potentials ranging from −100 mV to +60 mV in 20-mV increments, and tail currents were recorded at −30 mV (see inserted protocol). Cells expressing Kv1.1 and Kv1.3 channels responded to this voltage protocol with a fast-activating and a slow-inactivating current. For cells expressing KCNE4 together with Kv1.1 or Kv1.3 α -subunits, a significant reduction in the overall current level was observed. ( B ) Mean current values of HEK293 cells expressing either Kv1.1 or Kv1.3 alone or together with KCNE4. Peak current values were measured at +60 mV. For Kv1.1 and Kv1.1/KCNE4 an average current density of 195 ± 39 pA/pF ( n = 18) and 75 ± 17 pA/pF ( n = 19), respectively, was found. An even more pronounced reduction in the overall current level was observed for Kv1.3 and Kv1.3/KCNE4 with values of 91 ± 21 pA/pF ( n = 9) and 13 ± 4 pA/pF ( n = 12), respectively.

    Article Snippet: The cells were cotransfected with Kv1.1 or Kv1.1/KCNE4-c-myc, stained with antibodies raised against Kv1.1 channels and the c-myc epitop, and detected by confocal microscopy ( ).

    Techniques: Expressing, Transfection

    Kv current can be modified by delayed injection of KCNE4. ( A ) Oocytes injected with Kv1.3 cRNA were incubated 28 h, which resulted in a current level of 6.7 ± 0.7 μ A recorded at +60°mV. The oocytes were then separated in two pools and subsequently injected with either H 2 O ( filled squares ) or KCNE4 cRNA ( filled triangles ). Injection of KCNE4 resulted in a complete inhibition of the Kv1.3 current, whereas H 2 O-injected oocytes continued to express Kv1.3 current. Control oocytes injected with both Kv1.3 and KCNE4 cRNA at time 0 ( open squares ) did not at any time provide Kv1.3 specific current. ( B and C ) To obtain a higher time resolution for the inhibitory property of KCNE4, oocytes expressing either Kv1.1 or Kv1.3 channels were injected with KCNE4 cRNA 72 h after Kv injection (time 0 at graph). Expression levels of Kv current were subsequently followed every third hour. For both Kv1.1- and Kv1.3-expressing oocytes, a complete inhibition of the current could be observed within 24 h. All data points are an average of four recordings on independent oocytes.

    Journal: Biophysical Journal

    Article Title: KCNE4 Is an Inhibitory Subunit to Kv1.1 and Kv1.3 Potassium Channels

    doi:

    Figure Lengend Snippet: Kv current can be modified by delayed injection of KCNE4. ( A ) Oocytes injected with Kv1.3 cRNA were incubated 28 h, which resulted in a current level of 6.7 ± 0.7 μ A recorded at +60°mV. The oocytes were then separated in two pools and subsequently injected with either H 2 O ( filled squares ) or KCNE4 cRNA ( filled triangles ). Injection of KCNE4 resulted in a complete inhibition of the Kv1.3 current, whereas H 2 O-injected oocytes continued to express Kv1.3 current. Control oocytes injected with both Kv1.3 and KCNE4 cRNA at time 0 ( open squares ) did not at any time provide Kv1.3 specific current. ( B and C ) To obtain a higher time resolution for the inhibitory property of KCNE4, oocytes expressing either Kv1.1 or Kv1.3 channels were injected with KCNE4 cRNA 72 h after Kv injection (time 0 at graph). Expression levels of Kv current were subsequently followed every third hour. For both Kv1.1- and Kv1.3-expressing oocytes, a complete inhibition of the current could be observed within 24 h. All data points are an average of four recordings on independent oocytes.

    Article Snippet: The cells were cotransfected with Kv1.1 or Kv1.1/KCNE4-c-myc, stained with antibodies raised against Kv1.1 channels and the c-myc epitop, and detected by confocal microscopy ( ).

    Techniques: Modification, Injection, Incubation, Inhibition, Expressing

    KCNE4 inhibits Kv1.1 and Kv1.3 currents in Xenopus laevis oocytes. ( A ) Current traces ( left side ) and averaged IV-curves ( right side ) obtained in oocytes expressing Kv1.1 or Kv1.3 alone or together with KCNE4. Oocytes were clamped at −80 mV for 3 s, current traces were elicited by 2-s voltage steps to potentials ranging from −100 mV to +60 mV in 20 mV increments, and tail currents were recorded at −30 mV (see inserted protocol). Oocytes expressing only Kv1.1 or Kv1.3 channels responded to this voltage protocol with a fast-activating and a slow-inactivating current. For oocytes coinjected with KCNE4, a drastic reduction in the overall current level was observed. ( B ) Mean current values of oocytes expressing either Kv1.1 or Kv1.3 alone or together with KCNE4. Peak current values were measured at +60 mV. For Kv1.1 and Kv1.1/KCNE4, an average current level of 28.1 ± 2.1 μ A ( n = 20) and 2.3 ± 0.5 μ A ( n = 16), respectively, was found. A similar drastic reduction in the overall current level was observed for Kv1.3 and Kv1.3/KCNE4 with values of 12.9 ± 1.2 μ A ( n = 50) and 0.4 ± 0.1 μ A ( n = 33), respectively.

    Journal: Biophysical Journal

    Article Title: KCNE4 Is an Inhibitory Subunit to Kv1.1 and Kv1.3 Potassium Channels

    doi:

    Figure Lengend Snippet: KCNE4 inhibits Kv1.1 and Kv1.3 currents in Xenopus laevis oocytes. ( A ) Current traces ( left side ) and averaged IV-curves ( right side ) obtained in oocytes expressing Kv1.1 or Kv1.3 alone or together with KCNE4. Oocytes were clamped at −80 mV for 3 s, current traces were elicited by 2-s voltage steps to potentials ranging from −100 mV to +60 mV in 20 mV increments, and tail currents were recorded at −30 mV (see inserted protocol). Oocytes expressing only Kv1.1 or Kv1.3 channels responded to this voltage protocol with a fast-activating and a slow-inactivating current. For oocytes coinjected with KCNE4, a drastic reduction in the overall current level was observed. ( B ) Mean current values of oocytes expressing either Kv1.1 or Kv1.3 alone or together with KCNE4. Peak current values were measured at +60 mV. For Kv1.1 and Kv1.1/KCNE4, an average current level of 28.1 ± 2.1 μ A ( n = 20) and 2.3 ± 0.5 μ A ( n = 16), respectively, was found. A similar drastic reduction in the overall current level was observed for Kv1.3 and Kv1.3/KCNE4 with values of 12.9 ± 1.2 μ A ( n = 50) and 0.4 ± 0.1 μ A ( n = 33), respectively.

    Article Snippet: The cells were cotransfected with Kv1.1 or Kv1.1/KCNE4-c-myc, stained with antibodies raised against Kv1.1 channels and the c-myc epitop, and detected by confocal microscopy ( ).

    Techniques: Expressing

    Kv1.1 and KCNE4 colocalize in the cell membrane. Confocal scans of a HEK293 cell transfected with Kv1.1/ KCNE4-c-cmyc ( A ) and Kv1.1 ( B ). After fixation the cells were labeled with anti-Kv1.1 antibody ( A 1 and B ) and anti-c-myc antibody ( A 2). ( A 3) represents an overlay of the two labelings. For both proteins, large amounts of intracellular labeling were observed. However, some labeling could be detected in what appeared to be the plasma membrane ( arrow ). In many cases the pattern of staining of the two proteins overlapped ( arrow ). Scale bar 8 μ m.

    Journal: Biophysical Journal

    Article Title: KCNE4 Is an Inhibitory Subunit to Kv1.1 and Kv1.3 Potassium Channels

    doi:

    Figure Lengend Snippet: Kv1.1 and KCNE4 colocalize in the cell membrane. Confocal scans of a HEK293 cell transfected with Kv1.1/ KCNE4-c-cmyc ( A ) and Kv1.1 ( B ). After fixation the cells were labeled with anti-Kv1.1 antibody ( A 1 and B ) and anti-c-myc antibody ( A 2). ( A 3) represents an overlay of the two labelings. For both proteins, large amounts of intracellular labeling were observed. However, some labeling could be detected in what appeared to be the plasma membrane ( arrow ). In many cases the pattern of staining of the two proteins overlapped ( arrow ). Scale bar 8 μ m.

    Article Snippet: The cells were cotransfected with Kv1.1 or Kv1.1/KCNE4-c-myc, stained with antibodies raised against Kv1.1 channels and the c-myc epitop, and detected by confocal microscopy ( ).

    Techniques: Transfection, Labeling, Staining

    Summary of the role of Kv1.1 channels in regulating early postnatal neurogenesis in SGZ. The membrane potentials in type 1 radial glia-like neural stem cells and type 2a transit-amplifying progenitor cells are relatively hyperpolarized. In type 2b neural progenitors, cells lacking the Kv1.1 channel are more depolarized than wild types, further stimulating the proliferation of type 2b cells via activating the TrkB signaling pathway. The mature granule cells become hyperpolarized again.

    Journal: eLife

    Article Title: Kv1.1 channels regulate early postnatal neurogenesis in mouse hippocampus via the TrkB signaling pathway

    doi: 10.7554/eLife.58779

    Figure Lengend Snippet: Summary of the role of Kv1.1 channels in regulating early postnatal neurogenesis in SGZ. The membrane potentials in type 1 radial glia-like neural stem cells and type 2a transit-amplifying progenitor cells are relatively hyperpolarized. In type 2b neural progenitors, cells lacking the Kv1.1 channel are more depolarized than wild types, further stimulating the proliferation of type 2b cells via activating the TrkB signaling pathway. The mature granule cells become hyperpolarized again.

    Article Snippet: Furthermore, immunolabeling of Kv1.1 minus mice (IHC) is missing.

    Techniques:

    Functional expression of Kv1.1 channel in SGZ neural progenitor cells expressing Fezf2-GFP at postnatal 2 weeks. ( A ) In situ hybridization results showed that Kcna1 mRNA is expressed in Fezf2-GFP-positive neural progenitor cells. Inset is displayed at a higher magnification. ( B ) Kcna1 mRNA was not detected in the Kv1.1KO mouse, which served as a negative control. ( C ) Kv1.1 protein was expressed in the doublecortin (DCX)-expressing late-stage neural progenitor cells (arrows) but not Sox2-positive early-stage neural progenitor cells. Kv1.1 protein was highly expressed in the inhibitory interneurons (yellow arrows). ( D–J ) Pharmacological isolation of Kv1 currents in Fezf2-GFP-positive cells. Kv1.1 currents were elicited by trains of voltage steps from -80 mV to +40 mV in 10 mV increments from the holding potential of -80 mV in the absence ( D and G ) or presence of the Kv1-specific blocker dendrotoxin-k (DTX-K; 100 nM) ( E and H ). The DTX-K-sensitive currents were considered Kv1-mediated potassium currents ( F, I, and J ), which were much reduced in the Kv1.1KO mice. n = 4 cells from each phenotype. Scale bar = 20 μm in ( C ). Data are presented as mean ± SEM.

    Journal: eLife

    Article Title: Kv1.1 channels regulate early postnatal neurogenesis in mouse hippocampus via the TrkB signaling pathway

    doi: 10.7554/eLife.58779

    Figure Lengend Snippet: Functional expression of Kv1.1 channel in SGZ neural progenitor cells expressing Fezf2-GFP at postnatal 2 weeks. ( A ) In situ hybridization results showed that Kcna1 mRNA is expressed in Fezf2-GFP-positive neural progenitor cells. Inset is displayed at a higher magnification. ( B ) Kcna1 mRNA was not detected in the Kv1.1KO mouse, which served as a negative control. ( C ) Kv1.1 protein was expressed in the doublecortin (DCX)-expressing late-stage neural progenitor cells (arrows) but not Sox2-positive early-stage neural progenitor cells. Kv1.1 protein was highly expressed in the inhibitory interneurons (yellow arrows). ( D–J ) Pharmacological isolation of Kv1 currents in Fezf2-GFP-positive cells. Kv1.1 currents were elicited by trains of voltage steps from -80 mV to +40 mV in 10 mV increments from the holding potential of -80 mV in the absence ( D and G ) or presence of the Kv1-specific blocker dendrotoxin-k (DTX-K; 100 nM) ( E and H ). The DTX-K-sensitive currents were considered Kv1-mediated potassium currents ( F, I, and J ), which were much reduced in the Kv1.1KO mice. n = 4 cells from each phenotype. Scale bar = 20 μm in ( C ). Data are presented as mean ± SEM.

    Article Snippet: Furthermore, immunolabeling of Kv1.1 minus mice (IHC) is missing.

    Techniques: Functional Assay, Expressing, In Situ Hybridization, Negative Control, Isolation, Mouse Assay

    Kv1.1 channels do not affect embryonic hippocampal neurogenesis. Immunostaining indicated that there was no difference in dentate gyrus (DG) between wild-type and Kv1.1KO mice, in terms of Sox2+ progenitors ( A, D ) (p=0.4807, t -test), Ctip2+ postmitotic marker ( B, E ) (p=0.5365, t -test), or NeuN+ neurons ( C, F ) (p=0.4606, t -test) at E16.5. n = 5–6 for each genotype. Scale bar = 100 µm. Data are presented as mean ± SEM. n.s., no significant difference.

    Journal: eLife

    Article Title: Kv1.1 channels regulate early postnatal neurogenesis in mouse hippocampus via the TrkB signaling pathway

    doi: 10.7554/eLife.58779

    Figure Lengend Snippet: Kv1.1 channels do not affect embryonic hippocampal neurogenesis. Immunostaining indicated that there was no difference in dentate gyrus (DG) between wild-type and Kv1.1KO mice, in terms of Sox2+ progenitors ( A, D ) (p=0.4807, t -test), Ctip2+ postmitotic marker ( B, E ) (p=0.5365, t -test), or NeuN+ neurons ( C, F ) (p=0.4606, t -test) at E16.5. n = 5–6 for each genotype. Scale bar = 100 µm. Data are presented as mean ± SEM. n.s., no significant difference.

    Article Snippet: Furthermore, immunolabeling of Kv1.1 minus mice (IHC) is missing.

    Techniques: Immunostaining, Mouse Assay, Marker

    Loss of Kv1.1 channels depolarizes type 2b neural progenitor cells in SGZ at postnatal 2 weeks. ( A ) Based on the expression of the cell-fate markers, the neural progenitor cells in the sub-granular zone (SGZ) can be categorized into several developmental stages (see Figure 4 for detailed images). ( B ) Among all the Fezf2-GFP-positive cells in the SGZ, radial glia-like type 1 neural progenitor cells expressed predominantly Sox2, type 2a neural progenitor cells expressed both Sox2 and Tbr2 (white arrows), and type 2b progenitor cells expressed Tbr2. Post-mitotic immature neurons expressed doublecortin (DCX) and could also be identified as POMC-GFP-positive cells. ( C ) Among these cell types, type 2b cells were significantly increased in adult Kv1.1KO dentate gyrus compared to wild type (WT), according to the numbers of Tbr2-positive Fezf2-GFP-positive cells (n = 9 for each group; two-way ANOVA followed by Sidak's multiple comparisons test; p=0.0009, for type 2b cells). Scale bar = 20 μm.

    Journal: eLife

    Article Title: Kv1.1 channels regulate early postnatal neurogenesis in mouse hippocampus via the TrkB signaling pathway

    doi: 10.7554/eLife.58779

    Figure Lengend Snippet: Loss of Kv1.1 channels depolarizes type 2b neural progenitor cells in SGZ at postnatal 2 weeks. ( A ) Based on the expression of the cell-fate markers, the neural progenitor cells in the sub-granular zone (SGZ) can be categorized into several developmental stages (see Figure 4 for detailed images). ( B ) Among all the Fezf2-GFP-positive cells in the SGZ, radial glia-like type 1 neural progenitor cells expressed predominantly Sox2, type 2a neural progenitor cells expressed both Sox2 and Tbr2 (white arrows), and type 2b progenitor cells expressed Tbr2. Post-mitotic immature neurons expressed doublecortin (DCX) and could also be identified as POMC-GFP-positive cells. ( C ) Among these cell types, type 2b cells were significantly increased in adult Kv1.1KO dentate gyrus compared to wild type (WT), according to the numbers of Tbr2-positive Fezf2-GFP-positive cells (n = 9 for each group; two-way ANOVA followed by Sidak's multiple comparisons test; p=0.0009, for type 2b cells). Scale bar = 20 μm.

    Article Snippet: Furthermore, immunolabeling of Kv1.1 minus mice (IHC) is missing.

    Techniques: Expressing

    Classification of Fezf2-GFP progenitor cells in SGZ. Based on the expression of the cell-fate markers, neural progenitor cells in the sub-granular zone (SGZ) can be categorized into several developmental stages. Among all the Fezf2-GFP-positive cells ( A–F ), Sox2-positive type 1 cells ( A1–F1 ) are radial glia-like cells ( A–A4, C–C4 ) and can give rise to Sox2+/Tbr2+ type 2a transit-amplifying progenitor cells ( B–B4 , D–D4 ). Type 2a cells further differentiate into type 2b neuroblasts that are positive for Tbr2 only ( C–C4, F–F4 ). Type 1 ( A3 and D3 ) and type 2a ( B3 and E3 ) cells formed extensive syncytial connections with other cells from the same developmental stage, as indicated by the gap-junction-permeable avidin-neurobiotin ( A–B ) staining. Scale bar = 20 μm. ( G ) Type 1 and type 2a Fezf2-GFP-positive neural progenitor cells were hyperpolarized; by contrast, type 2b cells lacking Kv1.1 channels were significantly more depolarized than the wild-type (WT) cells (n = 14, 14, 9, 10, 9 (WT) and 28, 14, 10, 11, 8 (Kv1.1KO) for type 1 cells (Fezf2-GFP+/Sox2+), type 2a cells (Fezf2-GFP+/Sox2+/Tbr2+), type 2b cells (Fezf2-GFP+/Tbr2+), immature neurons (POMC-GFP+), and label-free mature neurons; (two-way ANOVA followed by Sidak's multiple comparisons test; p=0.02, for type 2b cells)).

    Journal: eLife

    Article Title: Kv1.1 channels regulate early postnatal neurogenesis in mouse hippocampus via the TrkB signaling pathway

    doi: 10.7554/eLife.58779

    Figure Lengend Snippet: Classification of Fezf2-GFP progenitor cells in SGZ. Based on the expression of the cell-fate markers, neural progenitor cells in the sub-granular zone (SGZ) can be categorized into several developmental stages. Among all the Fezf2-GFP-positive cells ( A–F ), Sox2-positive type 1 cells ( A1–F1 ) are radial glia-like cells ( A–A4, C–C4 ) and can give rise to Sox2+/Tbr2+ type 2a transit-amplifying progenitor cells ( B–B4 , D–D4 ). Type 2a cells further differentiate into type 2b neuroblasts that are positive for Tbr2 only ( C–C4, F–F4 ). Type 1 ( A3 and D3 ) and type 2a ( B3 and E3 ) cells formed extensive syncytial connections with other cells from the same developmental stage, as indicated by the gap-junction-permeable avidin-neurobiotin ( A–B ) staining. Scale bar = 20 μm. ( G ) Type 1 and type 2a Fezf2-GFP-positive neural progenitor cells were hyperpolarized; by contrast, type 2b cells lacking Kv1.1 channels were significantly more depolarized than the wild-type (WT) cells (n = 14, 14, 9, 10, 9 (WT) and 28, 14, 10, 11, 8 (Kv1.1KO) for type 1 cells (Fezf2-GFP+/Sox2+), type 2a cells (Fezf2-GFP+/Sox2+/Tbr2+), type 2b cells (Fezf2-GFP+/Tbr2+), immature neurons (POMC-GFP+), and label-free mature neurons; (two-way ANOVA followed by Sidak's multiple comparisons test; p=0.02, for type 2b cells)).

    Article Snippet: Furthermore, immunolabeling of Kv1.1 minus mice (IHC) is missing.

    Techniques: Expressing, Avidin-Biotin Assay, Staining

    Multinomial logistic regression for cell-type prediction. ( A ) Acute brain slice recording from the DG of an Fezf2-GFP mouse. ( B and C ) A multinomial logistic regression model was constructed using the biophysical characteristics of cells that were identified previously (same cohort of cells from Figure 3D ) as the training dataset. Two individual regression models were generated for wild-type ( B ) and Kv1.1KO ( C ) cells. The cell types of unknown cells were later classified according to the resting membrane potential, input resistance, and membrane capacitance. Heatmaps show the estimated probabilities for each cell type against membrane potential ( D ), input resistance ( E ), and membrane capacitance ( D ). The cell types were determined based on maximum likelihood. ( G ) Resting membrane potentials of wild-type and Kv1.1KO progenitor cells. The predicted type 2b cells lacking Kv1.1 channels were significantly more depolarized (two-way ANOVA, followed by Sidak's multiple comparisons test; p

    Journal: eLife

    Article Title: Kv1.1 channels regulate early postnatal neurogenesis in mouse hippocampus via the TrkB signaling pathway

    doi: 10.7554/eLife.58779

    Figure Lengend Snippet: Multinomial logistic regression for cell-type prediction. ( A ) Acute brain slice recording from the DG of an Fezf2-GFP mouse. ( B and C ) A multinomial logistic regression model was constructed using the biophysical characteristics of cells that were identified previously (same cohort of cells from Figure 3D ) as the training dataset. Two individual regression models were generated for wild-type ( B ) and Kv1.1KO ( C ) cells. The cell types of unknown cells were later classified according to the resting membrane potential, input resistance, and membrane capacitance. Heatmaps show the estimated probabilities for each cell type against membrane potential ( D ), input resistance ( E ), and membrane capacitance ( D ). The cell types were determined based on maximum likelihood. ( G ) Resting membrane potentials of wild-type and Kv1.1KO progenitor cells. The predicted type 2b cells lacking Kv1.1 channels were significantly more depolarized (two-way ANOVA, followed by Sidak's multiple comparisons test; p

    Article Snippet: Furthermore, immunolabeling of Kv1.1 minus mice (IHC) is missing.

    Techniques: Slice Preparation, Construct, Generated

    Kv1 antibodies target intracellular epitopes. (A) Twenty-seven of the remaining 62 patients with unknown VGKC complex antigenic targets precipitated either 125 I-αDTX-labelled Kv1.1/Kv1.2/Kv1.6 co-transfected HEK cell extracts (red circles) or

    Journal: Journal of Neurology, Neurosurgery, and Psychiatry

    Article Title: Intracellular and non-neuronal targets of voltage-gated potassium channel complex antibodies

    doi: 10.1136/jnnp-2016-314758

    Figure Lengend Snippet: Kv1 antibodies target intracellular epitopes. (A) Twenty-seven of the remaining 62 patients with unknown VGKC complex antigenic targets precipitated either 125 I-αDTX-labelled Kv1.1/Kv1.2/Kv1.6 co-transfected HEK cell extracts (red circles) or

    Article Snippet: 125 I-αDTX surface-binding studies on live Kv1-transfected HEK cells (see online S1C) and commercial antibodies to the extracellular domain of Kv1.1 ( B) confirmed adequate surface expression of Kv1.1, Kv1.2 and Kv1.6.

    Techniques: Transfection