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  • 94
    Thermo Fisher ksr xf
    Ksr Xf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ksr xf/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ksr xf - by Bioz Stars, 2021-04
    94/100 stars
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    99
    Thermo Fisher dulbecco s modified eagle s medium dmem ksr xf ksr xf knockout dmem medium
    Stage 1: induction of eye field from human embryonic stem cells (hESC). (A): Two days’ postseeding, SHEF1 hESC were treated with <t>Dulbecco's</t> modified <t>Eagle's</t> medium <t>KSR‐XF</t> alone (Control) or Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days (BMP4/7). Representative images showing immunocytochemistry for the pluripotency marker OCT4 and eye field markers LHX2, SOX11, PAX6, and MITF are shown. Dotted squares indicate the region that is magnified in the adjacent panels. Images are captured at ×10 magnification. (B): Quantitative polymerase chain reaction was carried out to measure expression of Mitf transcript in SHEF1 hESC treated with Induction Medium 1 alone (Control) or Induction Medium 1 supplemented with BMP4/7 (50 ng/ml, 100 ng/ml, 200 ng/ml; shown by triangle in order of ascending concentration); BMP4 (200 ng/ml); or BMP7 (200 ng/ml) ( n = 3, ±SD). Abbreviation: BMP, bone morphogenetic protein.
    Dulbecco S Modified Eagle S Medium Dmem Ksr Xf Ksr Xf Knockout Dmem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s modified eagle s medium dmem ksr xf ksr xf knockout dmem medium/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s modified eagle s medium dmem ksr xf ksr xf knockout dmem medium - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Stage 1: induction of eye field from human embryonic stem cells (hESC). (A): Two days’ postseeding, SHEF1 hESC were treated with Dulbecco's modified Eagle's medium KSR‐XF alone (Control) or Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days (BMP4/7). Representative images showing immunocytochemistry for the pluripotency marker OCT4 and eye field markers LHX2, SOX11, PAX6, and MITF are shown. Dotted squares indicate the region that is magnified in the adjacent panels. Images are captured at ×10 magnification. (B): Quantitative polymerase chain reaction was carried out to measure expression of Mitf transcript in SHEF1 hESC treated with Induction Medium 1 alone (Control) or Induction Medium 1 supplemented with BMP4/7 (50 ng/ml, 100 ng/ml, 200 ng/ml; shown by triangle in order of ascending concentration); BMP4 (200 ng/ml); or BMP7 (200 ng/ml) ( n = 3, ±SD). Abbreviation: BMP, bone morphogenetic protein.

    Journal: Stem Cells Translational Medicine

    Article Title: Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage

    doi: 10.5966/sctm.2016-0088

    Figure Lengend Snippet: Stage 1: induction of eye field from human embryonic stem cells (hESC). (A): Two days’ postseeding, SHEF1 hESC were treated with Dulbecco's modified Eagle's medium KSR‐XF alone (Control) or Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days (BMP4/7). Representative images showing immunocytochemistry for the pluripotency marker OCT4 and eye field markers LHX2, SOX11, PAX6, and MITF are shown. Dotted squares indicate the region that is magnified in the adjacent panels. Images are captured at ×10 magnification. (B): Quantitative polymerase chain reaction was carried out to measure expression of Mitf transcript in SHEF1 hESC treated with Induction Medium 1 alone (Control) or Induction Medium 1 supplemented with BMP4/7 (50 ng/ml, 100 ng/ml, 200 ng/ml; shown by triangle in order of ascending concentration); BMP4 (200 ng/ml); or BMP7 (200 ng/ml) ( n = 3, ±SD). Abbreviation: BMP, bone morphogenetic protein.

    Article Snippet: On day 2, cells were switched to Induction Medium 1: Dulbecco's modified Eagle's medium (DMEM) KSR‐XF (KnockOut DMEM medium [Thermo Fisher], supplemented with 20% KnockOut Serum Replacement Xeno‐Free [Thermo Fisher], 1% β‐Mercaptoethanol [Sigma‐Aldrich], 1% GlutaMax [Thermo Fisher], and 1% non‐essential amino acid solution [Thermo Fisher]), supplemented with two inhibitors (LDN/SB): 1 μM LDN‐193189 (Stemgent, Cambridge, MA, https://www.stemgent.com ) and 10 µM SB‐431542 (Sigma‐Aldrich).

    Techniques: Modification, Immunocytochemistry, Marker, Real-time Polymerase Chain Reaction, Expressing, Concentration Assay

    Stage 2: Generation of a mixed RPE population. (A): Two days’ postseeding, SHEF1 human embryonic stem cells were treated with Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days. At day 9, cells were replated in Dulbecco's modified Eagle's medium (DMEM) KSR‐XF alone (Control) or Induction Medium 3 (ActA) for 19 days. Quantitative polymerase chain reaction was used to measure expression of a panel of RPE markers. RPE generated by using spontaneous differentiation (Sp. RPE) were used for comparison ( n = 3, ±SD). (B): Representative images showing immunocytochemistry for CRALBP and MERTK in cells derived by directed differentiation and cultured as in panel A. Dotted squares indicate the region that is magnified in the panels below. RPE generated by spontaneous differentiation were used for comparison. Images are captured at ×10 magnification. (C): Quantification of CRALBP immunocytochemistry at day 28 in which cells during stage 2 were treated with DMEM KSR‐XF (Control) or Induction Medium 3 (ActA) for 3 days, 5 days, 10 days, or 19 days. For fewer than 19‐day ActA treatments, DMEM KSR‐XF was used for the remainder of the 19‐day period. RPE derived by using spontaneous differentiation (Sp. RPE) were used for comparison ( n = 3, ±SD). Abbreviations: ActA, Activin A; d, days; RPE, retinal pigment epithelium; Sp. RPE, spontaneous differentiation retinal pigment epithelium.

    Journal: Stem Cells Translational Medicine

    Article Title: Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage

    doi: 10.5966/sctm.2016-0088

    Figure Lengend Snippet: Stage 2: Generation of a mixed RPE population. (A): Two days’ postseeding, SHEF1 human embryonic stem cells were treated with Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days. At day 9, cells were replated in Dulbecco's modified Eagle's medium (DMEM) KSR‐XF alone (Control) or Induction Medium 3 (ActA) for 19 days. Quantitative polymerase chain reaction was used to measure expression of a panel of RPE markers. RPE generated by using spontaneous differentiation (Sp. RPE) were used for comparison ( n = 3, ±SD). (B): Representative images showing immunocytochemistry for CRALBP and MERTK in cells derived by directed differentiation and cultured as in panel A. Dotted squares indicate the region that is magnified in the panels below. RPE generated by spontaneous differentiation were used for comparison. Images are captured at ×10 magnification. (C): Quantification of CRALBP immunocytochemistry at day 28 in which cells during stage 2 were treated with DMEM KSR‐XF (Control) or Induction Medium 3 (ActA) for 3 days, 5 days, 10 days, or 19 days. For fewer than 19‐day ActA treatments, DMEM KSR‐XF was used for the remainder of the 19‐day period. RPE derived by using spontaneous differentiation (Sp. RPE) were used for comparison ( n = 3, ±SD). Abbreviations: ActA, Activin A; d, days; RPE, retinal pigment epithelium; Sp. RPE, spontaneous differentiation retinal pigment epithelium.

    Article Snippet: On day 2, cells were switched to Induction Medium 1: Dulbecco's modified Eagle's medium (DMEM) KSR‐XF (KnockOut DMEM medium [Thermo Fisher], supplemented with 20% KnockOut Serum Replacement Xeno‐Free [Thermo Fisher], 1% β‐Mercaptoethanol [Sigma‐Aldrich], 1% GlutaMax [Thermo Fisher], and 1% non‐essential amino acid solution [Thermo Fisher]), supplemented with two inhibitors (LDN/SB): 1 μM LDN‐193189 (Stemgent, Cambridge, MA, https://www.stemgent.com ) and 10 µM SB‐431542 (Sigma‐Aldrich).

    Techniques: Modification, Real-time Polymerase Chain Reaction, Expressing, Generated, Immunocytochemistry, Derivative Assay, Cell Culture

    Stage 3: Generation of a homogenous and functional retinal pigment epithelium (RPE) population. (A): Two days’ postseeding, SHEF1 human embryonic stem cells were treated with Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days. At day 9, cells were replated in Induction Medium 3 for a period of 19 days. At day 28, cells were replated in Dulbecco's modified Eagle's medium KSR‐XF and cultured for a period of 14 days. Representative images showing immunocytochemistry for indicated RPE markers are shown. Images are captured at ×10 magnification. (B): Cells cultured as in panel A were further dissociated and replated on transwells and cultured for a period of 10 weeks. A representative image showing en face immunocytochemistry for ZO‐1 is shown. Images are captured at ×20 magnification. (C): Spent medium from transwells described in panel B was collected from the top and bottom chambers and quantified for vascular endothelial growth factor (VEGF) and pigment epithelium‐derived factor (PEDF) concentration. The ratio of [VEGF]:[PEDF] was quantified in media from the two compartments ( n = 3, ±SD). (D): Representative confocal images showing phagocytosis of fluorescent bead (red) by RPE. ZO‐1 immunocytochemistry (green) shows the cell edge, and the presence of bead within the cell boundary indicates internalization by phagocytosis. Images are captured at ×63 magnification. Abbreviations: PEDF, pigment epithelium‐derived factor; VEGF, vascular endothelial growth factor.

    Journal: Stem Cells Translational Medicine

    Article Title: Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage

    doi: 10.5966/sctm.2016-0088

    Figure Lengend Snippet: Stage 3: Generation of a homogenous and functional retinal pigment epithelium (RPE) population. (A): Two days’ postseeding, SHEF1 human embryonic stem cells were treated with Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days. At day 9, cells were replated in Induction Medium 3 for a period of 19 days. At day 28, cells were replated in Dulbecco's modified Eagle's medium KSR‐XF and cultured for a period of 14 days. Representative images showing immunocytochemistry for indicated RPE markers are shown. Images are captured at ×10 magnification. (B): Cells cultured as in panel A were further dissociated and replated on transwells and cultured for a period of 10 weeks. A representative image showing en face immunocytochemistry for ZO‐1 is shown. Images are captured at ×20 magnification. (C): Spent medium from transwells described in panel B was collected from the top and bottom chambers and quantified for vascular endothelial growth factor (VEGF) and pigment epithelium‐derived factor (PEDF) concentration. The ratio of [VEGF]:[PEDF] was quantified in media from the two compartments ( n = 3, ±SD). (D): Representative confocal images showing phagocytosis of fluorescent bead (red) by RPE. ZO‐1 immunocytochemistry (green) shows the cell edge, and the presence of bead within the cell boundary indicates internalization by phagocytosis. Images are captured at ×63 magnification. Abbreviations: PEDF, pigment epithelium‐derived factor; VEGF, vascular endothelial growth factor.

    Article Snippet: On day 2, cells were switched to Induction Medium 1: Dulbecco's modified Eagle's medium (DMEM) KSR‐XF (KnockOut DMEM medium [Thermo Fisher], supplemented with 20% KnockOut Serum Replacement Xeno‐Free [Thermo Fisher], 1% β‐Mercaptoethanol [Sigma‐Aldrich], 1% GlutaMax [Thermo Fisher], and 1% non‐essential amino acid solution [Thermo Fisher]), supplemented with two inhibitors (LDN/SB): 1 μM LDN‐193189 (Stemgent, Cambridge, MA, https://www.stemgent.com ) and 10 µM SB‐431542 (Sigma‐Aldrich).

    Techniques: Functional Assay, Modification, Cell Culture, Immunocytochemistry, Derivative Assay, Concentration Assay