kshv genomic dna Search Results


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  • 95
    Zymo Research viral dna
    Viral Dna, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Covaris covaris e220 focused ultrasonicator
    Covaris E220 Focused Ultrasonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen qiaamp dna blood mini kit
    Qiaamp Dna Blood Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 22255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dnase i
    Dnase I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8697 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen dneasy blood and tissue kit
    Dneasy Blood And Tissue Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 54202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega pgl3 basic
    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
    Pgl3 Basic, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 12565 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc illumina miseq platform
    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
    Illumina Miseq Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 41500 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher total rna
    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
    Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 471882 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega pgl3 basic vector
    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
    Pgl3 Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 27770 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC bc 2216
    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
    Bc 2216, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bcp 1  (ATCC)
    94
    ATCC bcp 1
    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
    Bcp 1, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher power sybr green master mix
    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
    Power Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14092 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher salmon sperm dna
    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
    Salmon Sperm Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4924 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher pcr2 1 vector
    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
    Pcr2 1 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10955 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Zymo Research quick dna viral kit
    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
    Quick Dna Viral Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher sybr green
    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
    Sybr Green, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 40213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pacific Biosciences 20 kb smrtbell template libraries
    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
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    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
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    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
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    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
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    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
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    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter <t>pGL3-B</t> (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P
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    Image Search Results


    Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter pGL3-B (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P

    Journal: Journal of Virology

    Article Title: Sp3 Transcription Factor Cooperates with the Kaposi’s Sarcoma-Associated Herpesvirus ORF50 Protein To Synergistically Activate Specific Viral and Cellular Gene Promoters

    doi: 10.1128/JVI.01143-20

    Figure Lengend Snippet: Mapping the transcriptional start sites of the ORF56 transcripts and defining the regulatory elements in the ORF56 promoter. (A) Diagram of the ORF56/ORF57 gene structure and the ORF56 core promoter region. The numbers in the diagram are the nucleotide positions of the KSHV genome (GenBank accession no. NC_009333 ). A noncanonical TATA box motif, TATT, is located upstream of the transcriptional start sites of the ORF56 gene. (B) Representative 5′-RACE analysis of ORF56 transcripts expressed in sodium butyrate-induced HH-B2 cells. (C) Diagram of reporter constructs containing a full-length ORF56 promoter (ORF56p-FL) and its 5′ deletions. Point mutations at the TATT box motif of the ORF56 promoter, from 5′-TATTAC to 5′-TATAAA (consensus TATA box), are shown in the diagram. (D) Responsiveness of the ORF56p reporter constructs to sodium butyrate (SB) in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection and treatment, cells were harvested, and luciferase assays were performed. Data are indicated as fold activation of the luciferase activity of the reporter construct in the presence of SB treatment over the activity in the absence of SB treatment. (E) Responsiveness of the ORF56p reporter constructs to ORF50 overexpression in HH-B2, BC3, and HKB5/B5 cells. Forty-eight hours after transfection, cells were harvested, and luciferase assays were performed. Data are presented as fold activation of the luciferase activity of the reporter construct in the presence of the ORF50 expression vector over the activity in the presence of the empty vector control. For HKB5/B5 cells, it should be noted that all ORF56p-driven reporter constructs did not show higher ORF50 responsiveness than the control reporter pGL3-B (white bar). (F) Transcriptional activation of the indicated ORF56p reporter constructs by ORF50 in HKB5/B5 cells. The reporter constructs, including ORF56p-FL-(TATA), ORF56p-DA-(TATA), and ORF56p-DB-(TATA), contain a consensus TATA box motif (5′-TATAAA). *, P

    Article Snippet: The ORF56 promoter from positions −842 to +430, corresponding to nt 78254 to 79525 of the KSHV genome (GenBank accession no. NC_009333 ), was amplified by PCR using total DNA of HH-B2 cells as a template and cloned into pGL3-Basic (Promega).

    Techniques: Construct, Transfection, Luciferase, Activation Assay, Activity Assay, Over Expression, Expressing, Plasmid Preparation