klebsiella aerogenes atcc 13048 Search Results


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    Millipore cas no 13048
    Cas No 13048, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech 13048 1 ap
    13048 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech anti gpc4
    CD36 mediates proteasome-dependent ubiquitination of <t>GPC4.</t> a Protein levels of GPC4 in CRC cell lines with different CD36 expressions. b IF analysis of the co-localization of CD36 (green) and GPC4 (red) in RKO and CACO2 cell lines. c Co-immunoprecipitation (Co-IP) identified the interaction between CD36 and GPC4. d Time indicated cycloheximide (CHX, 20 μg/ml) treatment to compare the stability of GPC4 in indicated cell lines. Results are shown as mean ± SEM ( n = 3), * P < .05, ** P < .01, *** P < .001, based on two-way ANOVA. e MG132 (20 μM, 24 h) treatment on RKO and CACO2 cells with/without CD36 knockdown. f Co-IP determined endogenous ubiquitination and proteasome-dependent ubiquitination of GPC4. Source data are provided as a Source Data file
    Anti Gpc4, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Verlag GmbH 2017 wiley vch
    CD36 mediates proteasome-dependent ubiquitination of <t>GPC4.</t> a Protein levels of GPC4 in CRC cell lines with different CD36 expressions. b IF analysis of the co-localization of CD36 (green) and GPC4 (red) in RKO and CACO2 cell lines. c Co-immunoprecipitation (Co-IP) identified the interaction between CD36 and GPC4. d Time indicated cycloheximide (CHX, 20 μg/ml) treatment to compare the stability of GPC4 in indicated cell lines. Results are shown as mean ± SEM ( n = 3), * P < .05, ** P < .01, *** P < .001, based on two-way ANOVA. e MG132 (20 μM, 24 h) treatment on RKO and CACO2 cells with/without CD36 knockdown. f Co-IP determined endogenous ubiquitination and proteasome-dependent ubiquitination of GPC4. Source data are provided as a Source Data file
    2017 Wiley Vch, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nacalai ascorbic acid
    CD36 mediates proteasome-dependent ubiquitination of <t>GPC4.</t> a Protein levels of GPC4 in CRC cell lines with different CD36 expressions. b IF analysis of the co-localization of CD36 (green) and GPC4 (red) in RKO and CACO2 cell lines. c Co-immunoprecipitation (Co-IP) identified the interaction between CD36 and GPC4. d Time indicated cycloheximide (CHX, 20 μg/ml) treatment to compare the stability of GPC4 in indicated cell lines. Results are shown as mean ± SEM ( n = 3), * P < .05, ** P < .01, *** P < .001, based on two-way ANOVA. e MG132 (20 μM, 24 h) treatment on RKO and CACO2 cells with/without CD36 knockdown. f Co-IP determined endogenous ubiquitination and proteasome-dependent ubiquitination of GPC4. Source data are provided as a Source Data file
    Ascorbic Acid, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CD36 mediates proteasome-dependent ubiquitination of GPC4. a Protein levels of GPC4 in CRC cell lines with different CD36 expressions. b IF analysis of the co-localization of CD36 (green) and GPC4 (red) in RKO and CACO2 cell lines. c Co-immunoprecipitation (Co-IP) identified the interaction between CD36 and GPC4. d Time indicated cycloheximide (CHX, 20 μg/ml) treatment to compare the stability of GPC4 in indicated cell lines. Results are shown as mean ± SEM ( n = 3), * P < .05, ** P < .01, *** P < .001, based on two-way ANOVA. e MG132 (20 μM, 24 h) treatment on RKO and CACO2 cells with/without CD36 knockdown. f Co-IP determined endogenous ubiquitination and proteasome-dependent ubiquitination of GPC4. Source data are provided as a Source Data file

    Journal: Nature Communications

    Article Title: CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis

    doi: 10.1038/s41467-019-11662-3

    Figure Lengend Snippet: CD36 mediates proteasome-dependent ubiquitination of GPC4. a Protein levels of GPC4 in CRC cell lines with different CD36 expressions. b IF analysis of the co-localization of CD36 (green) and GPC4 (red) in RKO and CACO2 cell lines. c Co-immunoprecipitation (Co-IP) identified the interaction between CD36 and GPC4. d Time indicated cycloheximide (CHX, 20 μg/ml) treatment to compare the stability of GPC4 in indicated cell lines. Results are shown as mean ± SEM ( n = 3), * P < .05, ** P < .01, *** P < .001, based on two-way ANOVA. e MG132 (20 μM, 24 h) treatment on RKO and CACO2 cells with/without CD36 knockdown. f Co-IP determined endogenous ubiquitination and proteasome-dependent ubiquitination of GPC4. Source data are provided as a Source Data file

    Article Snippet: Cells were cultured in the confocal dish for 48–72 h and then washed with PBS for three times and fixed in 4% paraformaldehyde for 30 min, permeabilized with 0.5% Triton X-100, followed by blocking with goat serum, then cells were subjected to staining with anti-CD36 antibody (1:50, Santa Cruz), anti-β-catenin (1:100, Santa Cruz), anti-cmyc (1:100, GeneTex) or anti-GPC4 (1:100, Proteintech) antibody, followed by Alexa-488-conjugated goat anti-mouse secondary antibody (1:500, Bioss) or Alexa-647-conjugated goat anti-rabbit secondary antibody (1:500, Bioss) for imaging.

    Techniques: Immunoprecipitation, Co-Immunoprecipitation Assay

    The functional role of CD36 is dependent on GPC4. a Western blot of indicated proteins with/without MG132 treatment. Lamin B is a nuclear marker, GAPDH was loaded as a cytoplasmic marker. b Western blots of indicated proteins in SW480 and LoVo cells (LV-RFP vs. LV-CD36) with/without ectopic expression of GPC4. c IF analysis of β-catenin (red) location after forced expression of GPC4. d CCK8 tests. e Colony formation assays. f ATP production (left), glucose consumption (middle) and lactate production (right). Each experiment was performed in at least triplicate and results are presented as mean ± SEM. Student’s t -test or two-way ANOVA was used to analyze the data (* P < .05, ** P < .01, *** P < .001, **** P < .0001). Source data are provided as a Source Data file

    Journal: Nature Communications

    Article Title: CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis

    doi: 10.1038/s41467-019-11662-3

    Figure Lengend Snippet: The functional role of CD36 is dependent on GPC4. a Western blot of indicated proteins with/without MG132 treatment. Lamin B is a nuclear marker, GAPDH was loaded as a cytoplasmic marker. b Western blots of indicated proteins in SW480 and LoVo cells (LV-RFP vs. LV-CD36) with/without ectopic expression of GPC4. c IF analysis of β-catenin (red) location after forced expression of GPC4. d CCK8 tests. e Colony formation assays. f ATP production (left), glucose consumption (middle) and lactate production (right). Each experiment was performed in at least triplicate and results are presented as mean ± SEM. Student’s t -test or two-way ANOVA was used to analyze the data (* P < .05, ** P < .01, *** P < .001, **** P < .0001). Source data are provided as a Source Data file

    Article Snippet: Cells were cultured in the confocal dish for 48–72 h and then washed with PBS for three times and fixed in 4% paraformaldehyde for 30 min, permeabilized with 0.5% Triton X-100, followed by blocking with goat serum, then cells were subjected to staining with anti-CD36 antibody (1:50, Santa Cruz), anti-β-catenin (1:100, Santa Cruz), anti-cmyc (1:100, GeneTex) or anti-GPC4 (1:100, Proteintech) antibody, followed by Alexa-488-conjugated goat anti-mouse secondary antibody (1:500, Bioss) or Alexa-647-conjugated goat anti-rabbit secondary antibody (1:500, Bioss) for imaging.

    Techniques: Functional Assay, Western Blot, Marker, Expressing

    CD36 plays tumor-suppressive roles in vivo. a Subcutaneous xenograft tumor growth in nude mice (6 per group) were measured and compared (left) in SW480 (LV-RFP vs. LV-CD36) cell lines. Cell proliferation index was determined by the proportion of nuclear Ki-67–positive cells (right). b Representative images of IHC staining of CD36, GPC4, β-catenin, c-myc, GLUT1 and LDHA on tumor sections. Scale bar, 20 μm (40×). c Macroscopic appearance of livers and spleens of mice with intrasplenic inoculation (7 per group). d Representative images of H&E staining of liver sections in CD36-overexpressed and control group, Scale bar, 200 μm (4×), 50 μm (20×). e Subcutaneous xenograft tumor formation with RKO cells (shNC vs. shCD36), followed by treatment with intraperitoneal injection of PBS or 3-BrPA (5 mg/kg) or WZB117(5 mg/kg). f Representative images of IHC staining of Ki-67, HK2, and GLUT1 on tumor sections. Scale bar, 20 μm (40×). Cell proliferation index was quantified as before. Statistical results are shown as mean ± SEM, * P < .05, *** P < .001, **** P < .001, based on two-way ANOVA or Student’s t -test. Source data are provided as a Source Data file

    Journal: Nature Communications

    Article Title: CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis

    doi: 10.1038/s41467-019-11662-3

    Figure Lengend Snippet: CD36 plays tumor-suppressive roles in vivo. a Subcutaneous xenograft tumor growth in nude mice (6 per group) were measured and compared (left) in SW480 (LV-RFP vs. LV-CD36) cell lines. Cell proliferation index was determined by the proportion of nuclear Ki-67–positive cells (right). b Representative images of IHC staining of CD36, GPC4, β-catenin, c-myc, GLUT1 and LDHA on tumor sections. Scale bar, 20 μm (40×). c Macroscopic appearance of livers and spleens of mice with intrasplenic inoculation (7 per group). d Representative images of H&E staining of liver sections in CD36-overexpressed and control group, Scale bar, 200 μm (4×), 50 μm (20×). e Subcutaneous xenograft tumor formation with RKO cells (shNC vs. shCD36), followed by treatment with intraperitoneal injection of PBS or 3-BrPA (5 mg/kg) or WZB117(5 mg/kg). f Representative images of IHC staining of Ki-67, HK2, and GLUT1 on tumor sections. Scale bar, 20 μm (40×). Cell proliferation index was quantified as before. Statistical results are shown as mean ± SEM, * P < .05, *** P < .001, **** P < .001, based on two-way ANOVA or Student’s t -test. Source data are provided as a Source Data file

    Article Snippet: Cells were cultured in the confocal dish for 48–72 h and then washed with PBS for three times and fixed in 4% paraformaldehyde for 30 min, permeabilized with 0.5% Triton X-100, followed by blocking with goat serum, then cells were subjected to staining with anti-CD36 antibody (1:50, Santa Cruz), anti-β-catenin (1:100, Santa Cruz), anti-cmyc (1:100, GeneTex) or anti-GPC4 (1:100, Proteintech) antibody, followed by Alexa-488-conjugated goat anti-mouse secondary antibody (1:500, Bioss) or Alexa-647-conjugated goat anti-rabbit secondary antibody (1:500, Bioss) for imaging.

    Techniques: In Vivo, Immunohistochemistry, Staining, Injection

    AAV-mediated CD36 knockdown promotes CRC. a Macroscopic appearance of tumors in the large intestines of AOM-DSS-induced mice. Intestinal tumor numbers and tumor volumes were measured. b Representative images of IHC staining of indicated targets on tumor sections. Scale bar, 20 μm (40×). c Macroscopic appearance of tumors in the large intestine of Apc Min/+ mice, statistical analysis of tumor numbers and sizes in the colon and rectum. d Representative IHC staining of PCNA, cell proliferation index was calculated as before. All statistical results are shown as mean ± SEM, based on Student’s t -test, * P < .05, ** P < .01, *** P < .001. e Representative images of IHC staining of CD36, GPC4, β-catenin, c-myc, and downstream glycolytic genes GLUT1, HK2, PKM2 and LDHA on the tumor sections. Scale bar, 20 μm (40×). f Schematic diagram summarizing our working model, namely, CD36 can interact with and induce the proteasome-dependent ubiquitination of GPC4, thereby inhibiting β-catenin/c-myc signaling, followed by repressed glycolytic activity and colorectal tumor suppression. Source data are provided as a Source Data file

    Journal: Nature Communications

    Article Title: CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis

    doi: 10.1038/s41467-019-11662-3

    Figure Lengend Snippet: AAV-mediated CD36 knockdown promotes CRC. a Macroscopic appearance of tumors in the large intestines of AOM-DSS-induced mice. Intestinal tumor numbers and tumor volumes were measured. b Representative images of IHC staining of indicated targets on tumor sections. Scale bar, 20 μm (40×). c Macroscopic appearance of tumors in the large intestine of Apc Min/+ mice, statistical analysis of tumor numbers and sizes in the colon and rectum. d Representative IHC staining of PCNA, cell proliferation index was calculated as before. All statistical results are shown as mean ± SEM, based on Student’s t -test, * P < .05, ** P < .01, *** P < .001. e Representative images of IHC staining of CD36, GPC4, β-catenin, c-myc, and downstream glycolytic genes GLUT1, HK2, PKM2 and LDHA on the tumor sections. Scale bar, 20 μm (40×). f Schematic diagram summarizing our working model, namely, CD36 can interact with and induce the proteasome-dependent ubiquitination of GPC4, thereby inhibiting β-catenin/c-myc signaling, followed by repressed glycolytic activity and colorectal tumor suppression. Source data are provided as a Source Data file

    Article Snippet: Cells were cultured in the confocal dish for 48–72 h and then washed with PBS for three times and fixed in 4% paraformaldehyde for 30 min, permeabilized with 0.5% Triton X-100, followed by blocking with goat serum, then cells were subjected to staining with anti-CD36 antibody (1:50, Santa Cruz), anti-β-catenin (1:100, Santa Cruz), anti-cmyc (1:100, GeneTex) or anti-GPC4 (1:100, Proteintech) antibody, followed by Alexa-488-conjugated goat anti-mouse secondary antibody (1:500, Bioss) or Alexa-647-conjugated goat anti-rabbit secondary antibody (1:500, Bioss) for imaging.

    Techniques: Immunohistochemistry, Activity Assay