Journal: BMC Research Notes

Article Title: Two promoters in the esx-3 gene cluster of Mycobacterium smegmatis respond inversely to different iron concentrations in vitro

doi: 10.1186/s13104-017-2752-0

Figure Lengend Snippet: Growth curves of the WT, ΔMycP 3 mutant and the two complementation strains, ΔMycP3 ms ::pr1MycP3 ms and ΔMycP3 ms ::pr2MycP3 ms under Fe-free 7H9 ( a ), Fe-free Sauton’s media ( b ). The growth curves were done in triplicate, error bars show standard deviation

Article Snippet: Fe-free 7H9 (omitting ferric ammonium citrate) and Fe-free Sauton’s medium (3.5 mM KH2 PO4 (Sigma-Aldrich, USA, Catalogue No. NIST200B), 25 mM l -asparagine (Sigma-Aldrich, USA, Catalogue No. A0884), 10 mM citric acid (Sigma-Aldrich, USA, Catalogue No. 791725), 4 mM MgSO4 ·6H2 O (Sigma-Aldrich, USA, Catalogue No. 746452), 5% (v/v) glycerol, and 0.05% (v/v) Tween-80) were also used to monitor bacterial growth under iron-limiting condition.

Techniques: Mutagenesis, Mass Spectrometry, Standard Deviation

Journal: BMC Research Notes

Article Title: Two promoters in the esx-3 gene cluster of Mycobacterium smegmatis respond inversely to different iron concentrations in vitro

doi: 10.1186/s13104-017-2752-0

Figure Lengend Snippet: Growth curves of the WT, ΔMycP 3 mutant and the two complementation strains, ΔMycP3 ms ::pr1MycP3 ms and ΔMycP3 ms ::pr2MycP3 ms under Fe-depleted 7H9 ( a ), and Fe-depleted Sauton’s media ( b ). The growth curves were done in triplicate, error bars show standard deviation

Article Snippet: Fe-free 7H9 (omitting ferric ammonium citrate) and Fe-free Sauton’s medium (3.5 mM KH2 PO4 (Sigma-Aldrich, USA, Catalogue No. NIST200B), 25 mM l -asparagine (Sigma-Aldrich, USA, Catalogue No. A0884), 10 mM citric acid (Sigma-Aldrich, USA, Catalogue No. 791725), 4 mM MgSO4 ·6H2 O (Sigma-Aldrich, USA, Catalogue No. 746452), 5% (v/v) glycerol, and 0.05% (v/v) Tween-80) were also used to monitor bacterial growth under iron-limiting condition.

Techniques: Mutagenesis, Mass Spectrometry, Standard Deviation