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  • 94
    Roche kapa hifi hotstart polymerase chain reaction pcr kits
    Kapa Hifi Hotstart Polymerase Chain Reaction Pcr Kits, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    kapa hifi hotstart polymerase chain reaction pcr kits - by Bioz Stars, 2020-08
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    89
    Kapa Biosystems kapa hifi hotstart pcr kit
    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
    Kapa Hifi Hotstart Pcr Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 89/100, based on 380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 380 article reviews
    Price from $9.99 to $1999.99
    kapa hifi hotstart pcr kit - by Bioz Stars, 2020-08
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    91
    Kapa Biosystems kapa hifi hotstart readymix pcr kit
    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
    Kapa Hifi Hotstart Readymix Pcr Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 91/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa hifi hotstart readymix pcr kit/product/Kapa Biosystems
    Average 91 stars, based on 357 article reviews
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    kapa hifi hotstart readymix pcr kit - by Bioz Stars, 2020-08
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    92
    Roche kapa hifi hotstart readymix pcr kit
    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
    Kapa Hifi Hotstart Readymix Pcr Kit, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa hifi hotstart readymix pcr kit/product/Roche
    Average 92 stars, based on 99 article reviews
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    kapa hifi hotstart readymix pcr kit - by Bioz Stars, 2020-08
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    92
    Thermo Fisher kapa hifi hotstart readymix pcr kit
    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
    Kapa Hifi Hotstart Readymix Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Clinisciences kapa hifi hotstart readymix pcr kit
    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
    Kapa Hifi Hotstart Readymix Pcr Kit, supplied by Clinisciences, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    kapa hifi hotstart readymix pcr kit - by Bioz Stars, 2020-08
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    91
    Kapa Biosystems kapa hifi hotstart readmix pcr kit
    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
    Kapa Hifi Hotstart Readmix Pcr Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa hifi hotstart readmix pcr kit/product/Kapa Biosystems
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    kapa hifi hotstart readmix pcr kit - by Bioz Stars, 2020-08
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    92
    Kapa Biosystems kapa hifi hotstart uracil readymix pcr kit
    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
    Kapa Hifi Hotstart Uracil Readymix Pcr Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 92/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa hifi hotstart uracil readymix pcr kit/product/Kapa Biosystems
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    Sopachem kapa hifi hotstart readymix pcr kit
    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
    Kapa Hifi Hotstart Readymix Pcr Kit, supplied by Sopachem, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa hifi hotstart readymix pcr kit/product/Sopachem
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    kapa hifi hotstart readymix pcr kit - by Bioz Stars, 2020-08
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    92
    Millipore kapa hifi hotstart pcr kit
    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
    Kapa Hifi Hotstart Pcr Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    kapa hifi hotstart pcr kit - by Bioz Stars, 2020-08
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    86
    Kapa Biosystems kapa hifi hotstart pcr reagent kit
    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
    Kapa Hifi Hotstart Pcr Reagent Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa hifi hotstart pcr reagent kit/product/Kapa Biosystems
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    93
    Nippon Genetics kapa hifi hotstart readymix pcr kit
    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
    Kapa Hifi Hotstart Readymix Pcr Kit, supplied by Nippon Genetics, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa hifi hotstart readymix pcr kit/product/Nippon Genetics
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    kapa hifi hotstart readymix pcr kit - by Bioz Stars, 2020-08
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    Roche kapa hifi 2x hotstart readymix pcr kit
    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
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    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
    Kapa Hifi Hotstart Readymix 2x Pcr Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. <t>PCR</t> amplification was completed with the <t>KAPA</t> <t>HiFi</t> <t>HotStart</t> PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).
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    Different DNA polymerases can be used during <t>PCR.</t> In addition to Taq DNA polymerase and <t>KAPA</t> <t>HiFi,</t> Vent (exo-) DNA polymerase (New England Biolabs) and NEBNext (New England Biolabs) can be used during PCR. Each enzyme results in slightly different signal intensities from the incorporated degenerate bases. However, while Taq has higher signal than KAPA HiFi, for example, it also has a higher level of background noise due to its higher base mis-incorporation rate.
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    Image Search Results


    Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. PCR amplification was completed with the KAPA HiFi HotStart PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).

    Journal: American Journal of Human Genetics

    Article Title: MCM9 Mutations Are Associated with Ovarian Failure, Short Stature, and Chromosomal Instability

    doi: 10.1016/j.ajhg.2014.11.002

    Figure Lengend Snippet: Pathogenic Homozygous Recessive Variants in MCM9 Were Identified in Two Consanguineous Kurdish Families from Turkey (A and B) The MCM9 genotype is provided below each individual. WT denotes the wild-type allele. MT (for mutation) indicates either (A) MCM9 c.1732+2T > C or (B) MCM9 c. 394C > T. PCR amplification was completed with the KAPA HiFi HotStart PCR Kit with dNTPs (Kapa Biosystems) according to the manufacturer’s instructions (primers for A are 5′-TCTAGGAGGTCCCGAGATGG-3′ and 5′-CAAAGGCAGAGTGATTGCCG-3′; primers for B are 5′-GCCTGAGAGGCAAGTGAATTTAG-3′ and 5′-TACCTAAAACCAAGGATGTGGGA-3′). PCR products were sequenced at Beckman Coulter Genomics. Results were analyzed with Sequencher (Gene Codes Corporation). Sample chromatograms are shown (5′ to 3′ direction in A; 3′ to 5′ direction in B). Arrows point to the nucleotide peak of interest. Samples utilized for whole-exome sequencing (WES) are indicated by an asterisk. (C) MCM9 is encoded on chr6: 119,252,903–119,134,612 (UCSC Genome Browser hg19). The MCM9 c.1732+2T > C splice variant lies in the splice donor site of exon 9 (red arrow). The c.394C > T mutation lies near the 5′ end of exon 2 (blue arrow). Exons are indicated as full boxes (black). Alternative splice forms of MCM9 either include or exclude exon 11 (white). Introns are indicated as lines. (D) MCM9 consists of an N-terminal DNA binding domain and AAA + core domain. The c.1732+2T > C mutation is predicted to disrupt normal splicing after amino acid 577. The c.394C > T mutation causes insertion of a stop codon in the place of an arginine at position 132 (p.Arg132 ∗ ). This most likely results in a truncated protein if one is formed. The yellow box represents the arginine finger domain (RF), the pink box represents the Walker A motif (WA), and the deep green box represents the Walker B motif (WB).

    Article Snippet: Using the KAPA HiFi HotStart PCR Kit with dNTPs (Kapa Biosystems) and primers to amplify exons 7–12 (c.1151_1996), we expected to observe two previously reported wild-type products: an 846 bp product and a 700 bp product resulting from exon 11 skipping ( A; B, S3C, and A).

    Techniques: Mutagenesis, Polymerase Chain Reaction, Amplification, Sequencing, Variant Assay, Binding Assay, Western Blot

    Different DNA polymerases can be used during PCR. In addition to Taq DNA polymerase and KAPA HiFi, Vent (exo-) DNA polymerase (New England Biolabs) and NEBNext (New England Biolabs) can be used during PCR. Each enzyme results in slightly different signal intensities from the incorporated degenerate bases. However, while Taq has higher signal than KAPA HiFi, for example, it also has a higher level of background noise due to its higher base mis-incorporation rate.

    Journal: bioRxiv

    Article Title: NickSeq for genome-wide strand-specific identification of DNA single-strand break sites with single nucleotide resolution

    doi: 10.1101/867937

    Figure Lengend Snippet: Different DNA polymerases can be used during PCR. In addition to Taq DNA polymerase and KAPA HiFi, Vent (exo-) DNA polymerase (New England Biolabs) and NEBNext (New England Biolabs) can be used during PCR. Each enzyme results in slightly different signal intensities from the incorporated degenerate bases. However, while Taq has higher signal than KAPA HiFi, for example, it also has a higher level of background noise due to its higher base mis-incorporation rate.

    Article Snippet: 1 μL of DNA (with beads unless elution was performed) was the substrate for a 10 μL qPCR reaction using either Taq DNA polymerase or KAPA HiFi (KAPA HiFi HotStart PCR kit, Kapa Biosystems) according to the manufacturer’s protocol with barcoded P5 and P7 sequencing primers at 0.5 mM and with 1x EvaGreen dye (Biotium) and 1x ROX reference dye (Invitrogen) used to monitor DNA amplification.

    Techniques: Polymerase Chain Reaction

    Effect of degenerate nucleotides during PCR. a) Schematic of the oligonucleotides used to test the mutational outcome when dPTP and dKTP are incorporated into DNA and amplified by PCR. Four different oligonucleotides were generated, each with a nick directly 5’ of a different one of the four native dNTPs. The strand with the nick also contained a 5’ biotin-TEG modification to allow for purification of just that strand prior to PCR. PCR primers were designed to specifically amplify the region of the oligonucleotides containing the nicks and were tailed with 5’ sequences compatible with secondary PCR using barcoded P5 and P7 sequencing primers. b and c) Sequencing result of the four oligonucleotides following consecutive nick translations with dPTP plus dKTP and then with standard dNTPs, purification, and PCR with Taq DNA polymerase (b) or KAPA HiFi DNA polymerase (c) . The black triangles represent the locations of the nicks for each sample.

    Journal: bioRxiv

    Article Title: NickSeq for genome-wide strand-specific identification of DNA single-strand break sites with single nucleotide resolution

    doi: 10.1101/867937

    Figure Lengend Snippet: Effect of degenerate nucleotides during PCR. a) Schematic of the oligonucleotides used to test the mutational outcome when dPTP and dKTP are incorporated into DNA and amplified by PCR. Four different oligonucleotides were generated, each with a nick directly 5’ of a different one of the four native dNTPs. The strand with the nick also contained a 5’ biotin-TEG modification to allow for purification of just that strand prior to PCR. PCR primers were designed to specifically amplify the region of the oligonucleotides containing the nicks and were tailed with 5’ sequences compatible with secondary PCR using barcoded P5 and P7 sequencing primers. b and c) Sequencing result of the four oligonucleotides following consecutive nick translations with dPTP plus dKTP and then with standard dNTPs, purification, and PCR with Taq DNA polymerase (b) or KAPA HiFi DNA polymerase (c) . The black triangles represent the locations of the nicks for each sample.

    Article Snippet: 1 μL of DNA (with beads unless elution was performed) was the substrate for a 10 μL qPCR reaction using either Taq DNA polymerase or KAPA HiFi (KAPA HiFi HotStart PCR kit, Kapa Biosystems) according to the manufacturer’s protocol with barcoded P5 and P7 sequencing primers at 0.5 mM and with 1x EvaGreen dye (Biotium) and 1x ROX reference dye (Invitrogen) used to monitor DNA amplification.

    Techniques: Polymerase Chain Reaction, Amplification, Generated, Modification, Purification, Sequencing