Journal: PLoS ONE
Article Title: A tale of textiles: Genetic characterization of historical paper mulberry barkcloth from Oceania
Figure Lengend Snippet: Polymorphisms detected in the ITS-1 sequence of paper mulberry barkcloth textiles. Multiple alignment of 20 selected partial ITS sequences (50 bp) of textile samples. Image adapted from Bioedit 22.214.171.124. The reverse sequence (with primer ITS-C) is shown. The red arrow indicates the polymorphic nucleotide at the relative position 203 [ 21 ]. HM623778 corresponds to the ID of the B . papyrifera ITS region published in Genbank. M = double signal A/C at this position. Y = C or T; S = C or G; W = A or T. Bite Score (BS), Query Coverage (QC), E-values and identity percentage (Id) are indicated on the right. Extension names OT = sample amplified at the University of Otago using KAPA HiFi Hot Start DNA Polymerase; BM = sample amplified using GoTaq® G2 DNA Polymerase at the Faculty of Chemical and Pharmaceutical Sciences, University of Chile; MD = sample amplified using GoTaq® G2 Hot Start Flexi DNA Polymerase at the Faculty of Medicine, University of Chile, and P = sample amplified after extract purification.
Article Snippet: Analyses at the University of Otago were performed with KAPA HiFi Hot Start DNA Polymerase (Kapa Biosystems, Wilmington, MS, USA), while GoTaq® G2 Flexi DNA Polymerase and GoTaq® G2 Hot Start Polymerase (Promega, Madison, WI, USA) were used at the facilities in Chile.
Techniques: Sequencing, Amplification, Purification